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Citations for Bio-Rad :
1401 - 1450 of 7803 citations for Rat Indoleamine 2 3 Dioxygenase 1 IDO1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... Detergent was removed with four rounds of incubation with SM-2 beads (Bio-Rad) at 80mg beads per 1 mL of liposome suspension (2 hrs at 23°C twice ...
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bioRxiv - Biophysics 2020Quote: ... Triton X-100 was then removed by adding BioBeads SM-2 absorbent beads (BioRad) at a Bio-Beads/Triton X-100 ratio of 10 (wt/wt ...
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bioRxiv - Biochemistry 2020Quote: ... 2 to 15 µl of samples were dotted onto a nitrocellulose membrane (Bio-RAD). We used a concentration of 6E10 antibody of 1:2000 (Biolegend® ...
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bioRxiv - Bioengineering 2022Quote: ... using the primers listed in Supplementary Table 2 using SsoFast EvaGreen Supermix (Bio-Rad). The expression of the respective gene of interest was normalized to the samples’ respective GAPDH expression using the ΔCt or ΔΔCt method as indicated in the figures ...
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bioRxiv - Biochemistry 2021Quote: ... pH 7.5 and loaded onto a 2 ml CHT2-I hydroxyapatite column (Bio-Rad) equilibrated in 20 mM HEPES ...
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bioRxiv - Microbiology 2021Quote: ... cell lysates and immunoprecipitates on beads were resuspended in 2× Laemmli buffer (Bio-Rad) and subjected to SDS-PAGE and western blot analysis.
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bioRxiv - Bioengineering 2021Quote: ... 2% milk solution was prepared by dissolving nonfat dry milk powder (Bio-rad, UK) in distilled water and this solution was mixed with equal volume of 2% ...
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bioRxiv - Immunology 2022Quote: ... for 2 hours at 4 °C using Mini-trans blot wet tank transfer (BioRad). After transfer ...
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bioRxiv - Neuroscience 2019Quote: ... Bound proteins were eluted in 2 × Laemmli sample buffer (Bio-Rad, Cat# 161-0737) containing 5% 2-mercaptoethanol by heating at 95 °C for 5 minutes ...
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bioRxiv - Cancer Biology 2019Quote: ... sections were blocked with 2% milk powder (blotting grade; Bio-Rad Laboratories, Hercules, USA) in PBS for 30 minutes ...
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bioRxiv - Genomics 2021Quote: These reactions were undertaken on a DNA Engine Tetrad 2 Thermal Cycler (Bio-Rad), with MyTaq HS DNA polymerase ...
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bioRxiv - Plant Biology 2022Quote: ... Luminescence intensities mm-2 were evaluated using the Image Lab software (BioRad, Feldkirchen, Germany).
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bioRxiv - Microbiology 2022Quote: ... The cells:DNA mix was transferred to a 2 mm electroporation cuvette (Bio-Rad #1652082) and electroporated at 2500 kV ...
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bioRxiv - Molecular Biology 2022Quote: ... The gel was dried for 2 h on a Gel Dryer 583 (Bio-Rad) and visualized after appropriate exposure on a Phosphorimager (FLA-3000 Series ...
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bioRxiv - Immunology 2022Quote: ... and then blotted for 2 hours onto 0.45 μm supported nitrocellulose membranes (Bio-Rad). Membranes were blocked for one hour using 5% Bovine Serum Albumin (BSA ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... membranes were incubated for 2 minutes with Clarity Western ECL Blotting Substrate (Bio-Rad) and proteins visualized on ChemiDoc (Bio-Rad) ...
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bioRxiv - Cancer Biology 2023Quote: ... then 2 ul of normalized RNA were mixed with iTaq Universal SYBR (Bio-Rad) and primers for c-Myc (F ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and sheep polyclonal against Trans-Golgi network integral membrane protein 2 (TGN46; Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2023Quote: ... and re-suspended in 2x Laemmli sample buffer containing 2% SDS (Biorad, catalog #: 1610737). Protein concentration was measured by MicroBCA assay (Thermo Scientific ...
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bioRxiv - Biophysics 2023Quote: ... Afterwards the samples were incubated with Bio-Beads SM-2 sorbent (Bio-Rad Laboratories) overnight on the rolling bank at 4°C to remove the detergent (Rigaud et al ...
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bioRxiv - Biochemistry 2023Quote: ... detergent was removed by overnight incubation with activated Bio-Beads SM-2 (Bio-Rad). For purification of PhuR-containing nanodiscs ...
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bioRxiv - Plant Biology 2023Quote: ... Each reaction contained 5 μL of 2 x iQ SYBR Green supermix (Bio-Rad), 2 μL of diluted cDNA (10 times dilution for shoots and roots ...
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bioRxiv - Microbiology 2024Quote: ... the beads and total lysate were resuspended in 2× Laemmli sample buffer (Bio-Rad) supplemented with 5% 2-mercaptoethanol (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2024Quote: ... This mixture was transferred to a 2 mm ice cold electroporation cuvette (Bio-Rad). Transformation of WT P ...
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bioRxiv - Microbiology 2024Quote: ... The assays were incubated for 2 hours at 37 °C in a thermocycler (Biorad). Samples were then filtered through 3 kDa filter plates and derivatized with 4,5-dimethoxy-1,2-phenylenediamine hydrochloride (DMB) ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins bound to Dynabeads were eluted with 2 x Laemmli sample buffer (Bio-Rad) and subjected to immunoblot analysis ...
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bioRxiv - Microbiology 2023Quote: ... Xrn-1 digest samples were diluted 1:1 in 2X RNA loading dye (BioRad) and RNA was denatured at 80°C for 90 seconds ...
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bioRxiv - Immunology 2023Quote: ... The second kit used was the Bio-Plex Pro Serology Reagent Kit (Bio-Rad, Cat No. 12014777), with human IgA positive and negative controls (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... Excess antibody was washed away with 3 x TBST rinses before the membrane was incubated with anti-mouse secondary antibody (Bio-rad) conjugated to horseradish peroxidase at a 1:15000 dilution in TBST at 25°C for 1 hour ...
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bioRxiv - Molecular Biology 2020Quote: ... which is pre-equilibrated by Buffer A before use with rotation at 3 hr in 4°C in Econo-Pac Chromatography Columns (20 mL, Bio-Rad). After the unbounded fraction was discarded ...
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bioRxiv - Cell Biology 2020Quote: ... to pH 8.88 first for 5 min at 95 °C followed by 60 °C for 3 h on a T100 Thermal Cycler (Bio-Rad) with the heated lid set to 105 °C ...
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bioRxiv - Cell Biology 2020Quote: ... were added to this for a further 4 h before samples were centrifuged at 10,000 x g for 3 minutes and washed three times with RIPA buffer and re-suspended in laemmli buffer (Bio-Rad), boiled for 5 minutes ...
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bioRxiv - Microbiology 2020Quote: ... tuberculosis Rv1630 and Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) housekeeping gene was performed using 200 ng RNA in a T100 Thermal Cycler (Bio-Rad). The reaction was carried out with Reverse Transcription Reagents (Applied Biosystems ...
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bioRxiv - Genetics 2021Quote: ... separated using SDS-PAGE (4 to 12 % gradient gel at 150 V for 2.5-3 h) and transferred to a nitrocellulose membrane overnight (∼16 h) at 20 V using the Bio-Rad Transblot (Bio-Rad). Membranes were blocked in 5 % fat-free milk in 20 mmol/l Tris ...
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bioRxiv - Physiology 2022Quote: ... membranes were rinsed 3 times with 1X TBST and the blots were incubated in Clarity ECL western blot substrate solution (Bio-Rad) for 5 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... The resulting products were analyzed on a 3% agarose gel stained with ethidium bromide and visualized using ChemiDoc XRS+ (Bio-Rad). The proportion of each splice isoform was quantified using the ImageJ software.
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bioRxiv - Developmental Biology 2019Quote: ... Reverse primer: 5’-GCAGAAGAAGCAGACACAGC-3’) were PCR amplified and monitored using a CFX96 Touch Real-Time PCR detection system (Bio-Rad). Relative expression of PHETA1 transcripts was normalized to the expression of POLR2A and analyzed using standard delta delta Ct method ...
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bioRxiv - Bioengineering 2019Quote: Proliferation of MSCs was evaluated by determining the total cell count per well (n=3 wells/treatment/time-point) using a TC20 Automated Cell Counter (Bio-Rad).
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bioRxiv - Cell Biology 2019Quote: Home-made gels: The home-made Laemmli resolving gel (10 ml) contains 3 ml of 40% Acrylamide/Bis (cat#: 1610148, Bio-Rad), 2.5 ml of 1.5M Tris-HCl pH 8.8 (cat# ...
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bioRxiv - Cell Biology 2019Quote: ... Intact cells were divided into 100-μl aliquots and heated individually at different temperatures for 3 minutes in a PCR machine (Thermal cycler, BIO-RAD), followed by cooling for 2 minutes at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... containing the crude synaptosomes was resuspended and processed for western blotting in NP-40 lysis buffer as above with the following modifications: equal amounts of protein per sample (unboiled) were separated on 3-8% Tris-Acetate XT gel (Bio-Rad) in XT Tricine running buffer (Bio-Rad ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were washed again 3 times for 20 minutes and then developed using the BioRad imaging system (BioRad, Hercules, CA, USA).
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bioRxiv - Plant Biology 2021Quote: ... Quantitative RT-PCR was performed with gl3 specific primers (Supplemental Table 3) and iQ™ SYBR® Green Supermix (BioRad, USA) and conducted on a BioRad CFX with 96-well reaction blocks under the following PCR conditions ...
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bioRxiv - Microbiology 2020Quote: ... followed by ExtrAvidin alkaline phosphatase conjugate and alkaline phosphatase substrate: p-nitro blue tetrazolium chloride enhanced 5-bromo-4chloro-3-indolyl phosphate (Bio-Rad). The membranes with the transferred rgp120 were incubated with the HRP-conjugated V5 antibody (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... 5’-ATG GGT TCG CAT GGT CCT AAT GAC ACA C-3’) (Cousineau et al., 2022) and the GAPDH loading control (PrimePCR Probe assay with HEX probe, Bio-Rad). Each 20 µL reaction contained 500 ng of total RNA ...
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bioRxiv - Genetics 2022Quote: ... Membranes were washed 3 times for 10 min in PBST before detection using Clarity Western ECL Substrate (Bio-Rad 170-5061) and imaging on a ChemiDoc MP (Bio-Rad 17001402).
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bioRxiv - Bioengineering 2022Quote: Samples were washed in PBS and fixed in 3% paraformaldehyde (PFA) for 15 minutes at room temperature (RT) and permeabilized by 0.5% Triton x-100 (Bio-Rad, USA) for 10 minutes ...
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bioRxiv - Immunology 2020Quote: ... Samples were heated at 95°C for 3 minutes and run on a 8 – 16% or 4 – 20% TGX-Criterion gel (Bio-Rad). The separated proteins were transferred to PVDF membranes and blocked in PBS containing 0.2% fish skin gelatin (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... Specific primers against the genes of interest (Table 3) were designed and qPCR was performed to amplify the genes of interest using the CF96X Real Time System (BioRad, USA). The qPCR data were analyzed using the ΔΔCt method as described previously150 ...
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bioRxiv - Cell Biology 2021Quote: ... 1 ml of supernatant was diluted with 3 ml of distilled H2O and applied to freshly prepared Dowex columns (AG1-X8; Bio-Rad). Columns were washed twice with distilled H2O ...