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Citations for Bio-Rad :
1401 - 1450 of 1592 citations for Family With Sequence Similarity 46 Member B FAM46B Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Lysates of WT OSC were incubated with 1μg of Rabbit anti-Piwi polyclonal antibody (18) and 30 μl Surebeads Protein A Magnetic beads (Bio-Rad, 1614013). Immunoprecipitation was performed at 4°C overnight ...
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bioRxiv - Molecular Biology 2020Quote: Western blot detection was performed by enhanced chemiluminescence using HRP-coupled secondary antibodies and the Clarity or Clarity Max substrates (Bio-Rad). The signal was digitized using Amersham 600RGB imager as 16-bit grayscale TIFF files ...
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bioRxiv - Microbiology 2021Quote: ... for 1 h at room temperature and subsequently probed with appropriate secondary antibodies coupled to Horseradish peroxidase for 1 h at room temperature followed by chemiluminescent detection with ECL reagent (Bio-Rad).
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Activation of innate immune cGAS-STING pathway contributes to Alzheimer’s pathogenesis in 5×FAD micebioRxiv - Neuroscience 2022Quote: ... The membrane was incubated with anti-mouse or anti-rabbit HRP-conjugated secondary antibodies in 5% milk and bands were developed with Chemi-Doc XRS imaging (Bio-Rad). The primary antibodies used are listed in Supplementary Table 3.
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bioRxiv - Bioengineering 2022Quote: ... Antibody in the hybridoma supernatant was purified by passing through a column of DEAE Affigel blue (Bio-Rad, Sydney, NSW, Australia), dialysed into a Tris-saline buffer (Tris 10 mM ...
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bioRxiv - Physiology 2022Quote: ... Secondary antibodies were applied at room temperature for one hour and the blot was visualized using a ChemiDoc MP System (Bio-Rad). RBM20 bands were quantified with Image Lab software and normalized to the γ-tubulin loading control.
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies were detected by using horseradish peroxidase (HRP)-conjugated secondary antibodies (goat anti-mouse IgG (H+L)-HRP conjugate (1:10,000, Bio-Rad #1706516), goat anti-rabbit IgG (H+L)-HPR conjugate (1:10,000 ...
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bioRxiv - Immunology 2022Quote: ... were coated with 50 µL/well of 2 µg/mL of mouse anti-bovine IFN-γ mAb (CC330 Bio-Rad Antibodies) diluted in carbonate coating buffer (pH 9.6 ...
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bioRxiv - Plant Biology 2022Quote: ... and then incubated with 1:5000 dilution of horseradish peroxidase-conjugated goat anti-rabbit or anti-mouse secondary antibodies (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2022Quote: ... Membrane was washed for 5 min 3x with TBS-T and incubated with secondary goat anti-rabbit-HRP antibody (Bio-Rad) at a 1:10000 ...
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bioRxiv - Physiology 2022Quote: ... diluted 1:2500 in PBS-Tween 5% milk was used to detect primary antibodies bound to the blot by BIO-RAD ChemiDoc imaging system ...
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bioRxiv - Neuroscience 2023Quote: ... the membranes were washed and then incubated with the secondary antibody (Goat Anti-Rabbit IgG – HRP conjugate, Bio-Rad cat# 1706515) for one hour at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by incubation with appropriate horseradish peroxidase-conjugated secondary antibodies and detection using Clarity Western enhanced chemiluminescence substrate (Bio-Rad Laboratories).
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bioRxiv - Molecular Biology 2022Quote: ... the membranes were incubated with anti-mouse or –rabbit secondary antibodies tagged with horseradish peroxidase (1/10 000, Bio-Rad Laboratories) for one hour at room temperature and visualized by chemiluminescence (Amersham ECL prime western blotting detection reagent ...
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bioRxiv - Immunology 2022Quote: ... Blocking buffer was removed and 100 µL/well of samples and IFN-γ standards (recombinant bovine IFN-γ; Bio-Rad Antibodies) starting at 300 ng/mL followed by two-fold dilutions added ...
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bioRxiv - Immunology 2023Quote: ... The role of Mincle was investigated by incubating the cells with custom-made HuCAL anti-bovine Mincle CRD antibodies (AbD31621 and AbD31662) and HuCAL Fab-dHLX-FH Negative control (Bio-Rad) at 20 µg mL-1 for 15 min prior to addition of GFP-BCG.
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bioRxiv - Immunology 2023Quote: ... Bovine CD4+ T cells were isolated from PBMCs by magnetic-activated cell sorting (MACS) using a mouse anti-bovine CD4 antibody (clone CC8, Bio-Rad), anti-mouse IgG MicroBeads (Miltenyi Biotec) ...
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bioRxiv - Molecular Biology 2023Quote: ... blots were incubated with secondary antibodies for 1 h and were consequently developed using Clarity Western ECL and Clarity Max Western ECL substrates (Bio-Rad). Protein bands were visualized on the ChemidocTM MP imaging system (Bio-Rad).
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bioRxiv - Molecular Biology 2023Quote: ... Appropriate secondary HRP-linked antibody was incubated for 1 hour at room temperature and developed using Clarity Western ECL blotting substrate (BioRad, 1705061).
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were washed a further 3 times in TBS-T and bound antibodies were visualised using the Clarity Western ECL substrate (BioRad; #1705061) with a ChemiDoc MP system (BioRad) ...
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bioRxiv - Cell Biology 2023Quote: ... the membrane was incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (goat anti mouse #1721011; Bio-Rad or goat anti rabbit #1706515; BioRad) for 1 h at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... The membranes were subsequently probed with a primary antibody against β-ACTIN as a loading control or stained with Bio-Safe Coomassie stain (Biorad), dried ...
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bioRxiv - Molecular Biology 2023Quote: ... The HRP-linked secondary antibodies (CST: 7076S) were used by 1:4000 dilution and the blots were visualized with the ECL reagents (Bio-Rad).
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bioRxiv - Microbiology 2023Quote: ... Revelation was performed using a second antibody coupled to the HRP and the Clarity Western enhanced-chemiluminescence (ECL) substrate as describes by the manufacturer (Bio-Rad). Images were acquired and analyzed with the ImageQuant Las 4000 luminescent image analyzer.
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bioRxiv - Microbiology 2023Quote: Membrane was then washed three times with TBS-T and incubated with secondary antibody (Goat Anti-Rabbit IgG StarBright Blue 700 by Bio-Rad) diluted 1:3,000 in blocking solution for 45 min ...
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bioRxiv - Cell Biology 2023Quote: ... were used; and as secondary antibody HRP-conjugated anti-rabbit or anti-mouse (both 1:10 000; anti-rabbit (1706515; Bio-Rad); anti-mouse (170-6516 ...
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bioRxiv - Cancer Biology 2023Quote: ... After blocking with 5% (w/v) BSA or milk the membrane was incubated with the corresponding primary and secondary antibodies (Bio-Rad). The specific bands were analyzed using Thyphoon or ChemiDoc Imaging Systems (Bio-Rad).
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bioRxiv - Cell Biology 2023Quote: ... before being re-incubated with the secondary antibodies at room temperatures for 2 h and then subjected to the imaging of immunoblots by Bio-Rad instrument ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue morphology was visualized using fluorescent antibodies specific to lymphocyte and neuron specific molecular markers (anti-CD45 [Novus], anti-CD3 [Primary, Bio-Rad] ...
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bioRxiv - Cell Biology 2023Quote: ... Peroxidase-conjugated goat anti-mouse (170-6516) and peroxidase-conjugated goat anti-rabbit (1706515) secondary antibodies were purchased from Bio-Rad.
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bioRxiv - Bioengineering 2023Quote: ... followed by goat anti-mouse IgG (H+L)-horseradish peroxidase (HRP)-linked secondary antibody (1:1000, 1721011, Bio-Rad, RRID: AB_11125936). Detection of the horseradish peroxidase signal was carried out using Clarity Western enhanced chemiluminescence (ECL ...
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bioRxiv - Neuroscience 2023Quote: ... Equal amounts of proteins (20–100μg as optimal for each antibody) for each sample was loaded into 4-15% Mini-PROTEAN TGX Stain-Free Protein Gels (BioRad; #4568084) for electrophoresis ...
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bioRxiv - Immunology 2023Quote: ... and 100 μL of the secondary antibody solution - Goat Anti-Mouse IgG (H+L)-HRP Conjugate (Bio-Rad, Hercules, CA, USA) diluted in a 1:2,000 ratio in the antibody diluent - was then added and the plates were incubated for 1 hour in the dark at RT ...
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Comprehensive mutational analysis of the checkpoint signaling function of Rpa1/Ssb1 in fission yeastbioRxiv - Genetics 2023Quote: ... the membrane was blotted with the Chk1-pS345 antibody at the 1:3000 dilution for 3 h to detect the phosphorylated Chk1-Ser345 in ChemiDoc (Bio-Rad). The membrane was stripped ...
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bioRxiv - Microbiology 2023Quote: ... Signal detection was conducted with a goat anti-rabbit-HRP secondary antibody followed by development with Clarity ECL Substrate (Bio-Rad). Blots were imaged on Chemidoc XRS Imaging System (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... the membranes were washed three times (10 min each) and probed by incubation for 1 h with the appropriate secondary antibodies conjugated with horseradish peroxidase (Bio-Rad). The membranes were then washed three times (15 min each at room temperature) ...
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bioRxiv - Cell Biology 2023Quote: ... HRP-conjugated secondary antibodies associated signal was detected with ECL system and an enhanced chemiluminescence system (ChemiDoc Touch System, Bio-Rad). Quantification of the corresponding signal obtained was done using Image lab software.
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bioRxiv - Neuroscience 2023Quote: ... The secondary antibody was washed away with PBS-T and stained proteins were detected by chemiluminescence (Clarity western ECL, Bio-rad). The western blot signal was acquired using Amersham Image Quant8000.
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bioRxiv - Pathology 2023Quote: ... HRP- conjugated secondary antibody and ECL chemical luminescence reagents were used to detect immunoblot signal in the Gel-doc system (Bio-Rad).
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bioRxiv - Neuroscience 2023Quote: ... The antigen–antibody complexes were detected with SuperSignal West Pico PLUS Chemiluminescent Substrate (ThermoScientific) using the ChemiDoc XRS+ Imager System (Bio-Rad). Blots were exposed for 3 seconds ...
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bioRxiv - Physiology 2023Quote: ... The secondary antibodies were goat anti-mouse IgG-HRP and goat anti-rabbit IgG-HRP conjugate (Bio-Rad, Hercules, CA, USA) for western blotting ...
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bioRxiv - Physiology 2023Quote: ... Blotted bands were detected using HRP conjugated secondary antibodies goat anti-rabbit conjugated with HRP (#1706515, Bio-Rad, 1:15, 000). ImageJ was used to calculate the fluorescence density of each band ...
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bioRxiv - Cancer Biology 2023Quote: ... PVDF membranes were washed three times with tween 20-containing tris buffered saline before incubation with HRP-conjugated secondary antibodies (Bio-Rad). Images were detected using a ChemiDoc MP Imaging System (Bio-Rad).
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bioRxiv - Cancer Biology 2023Quote: ... membranes were incubated with HRP-linked secondary antibodies and developed with Clarity or ClarityMax chemiluminescent substrates using a ChemiDoc XRS+ imager (Bio-Rad). (See Supplementary Methods Table 3 for antibody information).
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bioRxiv - Bioengineering 2023Quote: ... probed with a goat anti-mouse or goat anti-rabbit IgG H+ L-HRP conjugated secondary antibody (1:5000-1:10000) (Bio-Rad) for 1 hour ...
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bioRxiv - Physiology 2024Quote: ... was used to wash the membranes before incubating with goat anti-rabbit horseradish peroxidase conjugated secondary antibody at 1:5000 dilution (1706515, Bio-Rad) for 90 min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... the membrane was incubated with primary antibodies at 4°C overnight and subsequently incubated with HRP-conjugated secondary antibodies at room temperature for 1 h and all images were acquired using a ChemiDoc XRS+ system (Bio-Rad). Mouse anti-GAPDH antibody was used at a 1:2000 dilution with TBST containing 3% BSA (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were visualized using horseradish-peroxidase-conjugated secondary antibodies followed by chemiluminescence detection with ECL (Clarity Western ECL substrate, Bio-Rad).
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bioRxiv - Cell Biology 2023Quote: ... followed by washes at room temperature (1 x 15 min, 2 x 5 min) and incubation with HRP-conjugated secondary antibodies (Bio-Rad) for 1 h ...
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bioRxiv - Microbiology 2024Quote: ... GFP-tagged proteins were detected using anti-GFP (Wako, mFX75, 1:5 000 dilution) antibodies combined with Goat Anti-Mouse IgG (H+L) HRP Conjugate (Bio-Rad). Images were acquired using Amersham Imager 600 (GE Healthcare ...