Labshake search
Citations for Bio-Rad :
1351 - 1400 of 4760 citations for 5 Nitro 1H benzo de isoquinoline 1 3 2H dione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2022Quote: Each 10 μl PCR reaction consisted of 5 μl of SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, Germany), 250 nM of forward and reverse primers ...
-
bioRxiv - Cell Biology 2022Quote: ... Total protein (5-10 ug) was loaded in 15 well pre-casted gradient gel (456-8086, Bio-Rad). After electrophoresis ...
-
bioRxiv - Cell Biology 2023Quote: ... 20 μg of protein extracts were loaded and run on 5-20% gradient pre-cast gels (Bio-Rad) before being transferred onto a 0.45 μM nitrocellulose membrane (Amersham ...
-
bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked with Tris-buffered saline with 0.05% Tween-20 (TBST) containing 5% dried nonfat milk (Biorad). Primary antibodies were diluted in TBST containing 5% bovine serum albumin and 0.02% NaN3 ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were stained with 5 μM MitoSOX for 20 minutes and analyzed using ZE5 Cell Analyzer (Bio-rad).
-
bioRxiv - Immunology 2023Quote: ... Lysates were heated at 95 °C for 5 min together with Laemmli sample buffer (Bio-Rad, catalog #1610747). Lysates were resolved using a 4% to 15% Criterion TGX Stain-Free Precast gel (Bio-Rad ...
-
bioRxiv - Microbiology 2023Quote: ... folic acid 500 μg/L) or on Columbia agar solid support enriched with 5% horse blood (COH) (Biorad). Anoxic conditions were generated in Gaspack (BD ...
-
bioRxiv - Cell Biology 2023Quote: ... according to the manufacturer’s instructions and an iQ™5 real-time PCR System (Bio-Rad Laboratories, USA). All reactions were pre-denaturation at 95°C for 3 min ...
-
bioRxiv - Developmental Biology 2023Quote: ... for 5 minutes each and then blocked for one hour in 0.5% Blotting-Grade Blocker (Bio-Rad, 1706404) diluted in TBST at room temperature ...
-
bioRxiv - Microbiology 2024Quote: ... Real-time quantitative PCR was performed using the MyiQ™5 Real-Time PCR Detection System (Bio-Rad) using the following thermal cycle parameters ...
-
bioRxiv - Cell Biology 2024Quote: ... cDNA was diluted 5-fold and qRT-PCR was performed using iTaq Universal SYBR Green Supermix (Bio-Rad) in CFX Connect Real-time System (Bio-Rad) ...
-
bioRxiv - Immunology 2024Quote: ... 5 ng of cDNA was then subjected to qPCR analysis using iQ SYBR Green super-mix reagent (BioRad). Gene expression levels were normalized to a housekeeping gene (TATA-binding protein or HPRT) ...
-
bioRxiv - Physiology 2024Quote: ... Protein (5-10 µg) was separated by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) (Bio-Rad, USA) in 26-well 4–15% Criterion™ TGX Stain-Free™ Protein Gels (Bio-Rad ...
-
bioRxiv - Molecular Biology 2024Quote: ... The formazan crystals formed by the cells were dissolved by adding 100 µL of 5% SDS (Bio-Rad) in 1N HCl (Thermo Fisher ...
-
bioRxiv - Biochemistry 2024Quote: ... Integrity of the annealed oligonucleotides was determined by application to Mini-PROTEAN native 5% polyacrylamide TBE gel (Biorad), staining with SYBR safe DNA gel stain (Thermo Fischer Scientific ...
-
bioRxiv - Biochemistry 2024Quote: ... Pooled peak fractions were then loaded onto a 5 ml Bio-Scale Mini CHT Type I Cartridge (Biorad) in 10 mM NaH2PO4 Buffer and eluted sequentially with 2CV 40 mM NaH2PO4 Buffer ...
-
bioRxiv - Genetics 2024Quote: ... Electroporation was performed at 2.5 kV for 5 ms in a MicroPulser electroporator (Bio-Rad, Hercules, California, USA) using 0.2 cm cuvettes ...
-
bioRxiv - Cancer Biology 2024Quote: ... at 95 degrees Celsius for 5 minutes before loading onto 10% Mini-PROTEAN TGX precast protein gel (BioRad). PageRuler Plus Pre-stained Protein Ladder (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2024Quote: ... and 5 µg total protein was loaded on each lane of a Mini Protean TGX Gel (BioRad, #4561093) together with a standard (WesternC ...
-
bioRxiv - Biochemistry 2024Quote: ... boiled for 5 min and then resolved on 4-20% (w/v) gradient SDS-PAGE gels (Bio-Rad) with Tris-glycine buffer ...
-
bioRxiv - Physiology 2021Quote: ... and mixed 1:1 with 2x Laemmli buffer (Bio-Rad) plus 5% β-mercaptoethanol ...
-
bioRxiv - Microbiology 2021Quote: ... The blot was stained with 1:1 enhancer:substrate reagents (BioRad) for 5 min ...
-
bioRxiv - Neuroscience 2021Quote: ... Each sample diluted 1:1 with Laemmli Sample Buffer (BioRad) mixed with 350 mM DTT ...
-
bioRxiv - Neuroscience 2023Quote: ... Protein was denatured with 1:1 Laemlli loading buffer (BIORAD) by boiling for 10 minutes on a dry bath (Thermolyne Dri-Bath ...
-
bioRxiv - Neuroscience 2022Quote: ... Each sample diluted 1:1 with Laemmli Sample Buffer (BioRad) mixed with 350 mM DTT ...
-
bioRxiv - Neuroscience 2022Quote: ... Each sample diluted 1:1 with Laemmli Sample Buffer (BioRad) mixed with 350 mM DTT ...
-
bioRxiv - Genetics 2023Quote: ... 1:500 to 1:1000 anti-rabbit (BioRad, 172-1019). Signal was detected using Pierce ECL Western Blotting Substrate (Thermo Scientific ...
-
bioRxiv - Biochemistry 2023Quote: ... The collected cells were mixed 1:1 with TrypanBlue (BioRad) and counted.
-
bioRxiv - Immunology 2021Quote: ... (BioRad Cl:A3-1) and goat anti-rat IgG cross-absorbed Alexa Fluor® 488 secondary antibody (ThermoFisher ...
-
bioRxiv - Cancer Biology 2024Quote: ... 1 (BioRad Laboratories) and normalized the loading control.
-
bioRxiv - Systems Biology 2021Quote: Differential expression of ten selected transcripts in response to virus at 3-L1 stage was examined using Droplet Digital PCR (ddPCR) (Bio-Rad, Hercules, CA). Because of the difficulty in obtaining a large amount of gut RNA samples and the limited sensitivity of conventional qPCR technique to detect gene expression at a low level ...
-
bioRxiv - Neuroscience 2022Quote: ... The embryoid media was aspirated and 15 to 20 embryoid bodies were plated per well in 6-well plates and cultured in 3 mL hematopoetic medium (2 mM GlutaMax, 1x Anti-Anti, 55 mM 2-mercaptoethanol (BioRad; Cat. No. 1610710), 100 ng/mL M-CSF ...
-
bioRxiv - Cell Biology 2021Quote: ... Band density was normalized according to the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) content and analyzed using Image Lab software (Bio-Rad, California, USA) (29).
-
bioRxiv - Cell Biology 2020Quote: ... fixed in 4% paraformaldehyde for 2 h at 4 °C and embedded in PBS containing 3% low-melting point agarose (Bio-Rad, California, USA). The agarose gels containing the fixed organoids were processed in a standard automated tissue histology processor ...
-
bioRxiv - Plant Biology 2021Quote: ... Membranes were washed 3 x 10min in TBS + Tween 20 before proteins were detected by Immuno-Star luminol-peroxide (Bio-Rad, #170-5070). Bio-Rad Chemi-doc was used to visualize and quantify proteins with densitometry.
-
bioRxiv - Cancer Biology 2020Quote: ... incubated with 3% H2O2 for 15 min at room temperature and then incubated with goat anti-rabbit IgG-HRP (Bio-Rad 170-6515) secondary antibody diluted 1:300 in the blocking buffer for 1 hour at room temperature ...
-
bioRxiv - Molecular Biology 2023Quote: ... Samples were denatured at 95 °C for 3 minutes prior to loading onto a 16.5% Mini-PROTEAN® Tris-Tricine Gel (Biorad Cat. No. 4563066). Gels were transferred to a nitrocellulose membrane (Millipore Sigma Cat ...
-
bioRxiv - Cancer Biology 2023Quote: ... The cell suspension then was divided equally and heated for 3 min at indicated temperatures on T100™ Thermal Cycler (Bio-Rad Laboratories). Cells were snap-frozen and thawed twice and centrifuged at 17.000 g for 15 min at 4°C ...
-
bioRxiv - Genomics 2024Quote: ... 4.5 µl of sample were used to generate single cell RNA-Seq libraries by following the Illumina Bio-Rad SureCell WTA 3’ Library Prep Guide (Illumina, San Diego CA and Bio-Rad, Hercules, CA) and using the ddSEQ Isolator for Single Cell Sequencing (Bio-Rad ...
-
bioRxiv - Biophysics 2023Quote: ... Migration of the DNA in the gel was visualized via the fluorescent Cy-3 label (ChemiDoc™ MP Imaging System, BIO-RAD, USA). Protein-DNA complexes were transferred from the gel to the PVDF membrane (Immun-Blot® PVDF Membrane ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... for DpnII digested DNA or primers 3 and 4 (table S3) for NlaIII digested DNA on a thermal cycler (Bio-Rad C1000 Touch) with the block settings shown in table 1 ...
-
bioRxiv - Microbiology 2023Quote: ... for the selected clinical isolates using the indicated concentrations of antibiotics via an alamar blue reduction assay completed as described but without shaking.8 After 3 days of incubation with the alamar blue reagent (BioRad, Hercules, CA, USA), the MIC was identified as the lowest concentration of drug that inhibited the reagent color change as determined visually.
-
bioRxiv - Microbiology 2024Quote: ... and R-tot (5′-CTGCTGCCTCCCGTAGGAGT-3′) in the Rotor Gene 6000 thermocycler (Corbett Research, Sydney, Australia) with the iTaq Universal SYBR Green Supermix (Bio-Rad, Reinach, Switzerland).
-
bioRxiv - Biophysics 2024Quote: ... His-tagged protein was bound to 3 mL HisPur™ Ni-NTA Resin (Thermo 88221) equilibrated with lysis buffer in a glass Econo-Column® (Bio-Rad) by flowing through the clarified lysate ...
-
bioRxiv - Molecular Biology 2024Quote: ... 3-10 μg total protein was loaded and run on AnykD™ Criterion™ TGX™ Precast acrylamide protein gels (Biorad cat#5671125) then transferred to nitrocellulose (Biorad cat#1704159) ...
-
bioRxiv - Biochemistry 2021Quote: ... Equal amounts (1 mg) of protein were diluted 1:1 in sample loading buffer (2x, Bio-Rad, UK) for each extraction method and loaded onto a 12% Tris-glycine pre-cast SDS gel (Invitrogen ...
-
bioRxiv - Neuroscience 2021Quote: ... secondary antibodies (1:10000; 1:50000 for anti-H3 antibody, BioRad) in 5% milk/PBST at RT ...
-
bioRxiv - Cell Biology 2020Quote: ... and 1:500 to 1:1000 anti-rabbit (BioRad, 172–1019). Signal was detected using Pierce ECL Western Blotting Substrate (Fisher ...
-
bioRxiv - Cancer Biology 2022Quote: ... and F4/80 (dilution 1:200, clone A3-1, Bio-Rad); ER2 EDTA for CD3 (dilution 1:100 ...
-
bioRxiv - Neuroscience 2023Quote: ... secondary antibodies (1:10000; 1:50000 for anti-Gapdh antibody, BioRad) in 5% milk/PBST at RT ...