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Citations for Bio-Rad :
1301 - 1350 of 2599 citations for 5 Bromobenzothiazole 2 sulfonic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... incubated 5 min at 95 °C and separated on gradient gels (BioRad 4-12% TGX pre-cast gels). The modified proteins could be detected with an infrared imager (Odyssey ...
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bioRxiv - Immunology 2020Quote: ... and transferred onto a PVDF membrane (Thermo Fisher Scientific).The membranes were blocked by 5% skim milk in TBST (Tris-buffered saline /0.1% Tween 20, Bio-Rad) and then incubated with antibodies to collagen a1 (IV ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were separated along with 5 µl Precision Plus Protein All Blue Prestained Protein Standards (Bio-Rad, 1610373) at 80-120 V in SDS-PAGE running buffer containing 25 mM Tris ...
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bioRxiv - Microbiology 2023Quote: ... the membrane was washed three times for 5 min each with TBST and incubated with StarBright B520 (BioRad) as the secondary antibody at 1:2,500 in 5% nonfat dry milk in TBST 1 h at room temperature ...
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bioRxiv - Physiology 2023Quote: ... membranes were housed in clear blotting boxes and blocked in 5% blocking buffer in TBS-T (BIORAD 1706404) for 40 min at +25°C ...
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bioRxiv - Molecular Biology 2023Quote: ... heated at 95°C for 5 min then run on a 4-12% Bis-Tris gel (BioRad 3450125). The input sample is identical to the initial solution containing protein in 60 μL 1x selection buffer prior to capture with 20 passages over the ME200 tip ...
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bioRxiv - Cell Biology 2023Quote: ... and resolved by SDS-PAGE using Criterion XT Bis-Tris precast gels (4-12%; BioRad, 3450123/4/5) and XT-MES1X as running buffer (stock 10X ...
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bioRxiv - Neuroscience 2022Quote: ... then washed 6 x 5 minutes with TBS-T before developing with Clarity Western ECL Substrate (Bio-rad).
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bioRxiv - Microbiology 2022Quote: ... 0.02% bromophenol) and 5 µL of each sample were loaded onto an AnykD precast polyacrylamide gel (Bio-Rad). The gel was run in 25 mM Tris ...
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bioRxiv - Cell Biology 2023Quote: ... heated to 60 LJC for 5 min and electrophoresed into a 12% Tris-HCl Criterion gel from BioRad at 50 V for 20 min or until the dye front moved just below the bottom of the sample wells ...
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bioRxiv - Neuroscience 2023Quote: ... with 5% β-Mercaptoethanol then loaded on 4-20% Criterion TGX Precast Midi protein gels (Bio-Rad #5671094). The gels were run in TGS buffer (1x ...
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bioRxiv - Neuroscience 2023Quote: ... Blocking was performed at room temperature for 60 min with blocking buffer: 5% nonfat dry milk (Bio-Rad) in TBST (150 mM NaCl ...
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bioRxiv - Plant Biology 2023Quote: ... and 5 μL of diluted cDNA was implemented for real-time quantitative PCR using the SYBG reagent (Biorad). The PCR reaction was performed in the thermocycler (C1000 TouchTM Thermal Cycler ...
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bioRxiv - Bioengineering 2023Quote: 5 uL of each sample was pipetted into a Hard-Shell 384-well clear microplate (Bio-Rad #HSP3805), and fluorescence and absorbance readings were taken by a Synergy H1 microplate reader (BioTek) ...
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bioRxiv - Cell Biology 2023Quote: ... and 5-10 μg aliquots fractionated by SDS-PAGE then transferred to nitrocellulose (0.45 μm) membrane (Bio-Rad). Membranes were blocked for 1 h at room temperature in 5% BSA in 1x Tris-buffered saline + 0.1% Tween 20 (TBST) ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked for 1 h at room temperature with 5% non-fat dry milk (Bio-Rad Laboratories) in Tris-buffered saline (TBS ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Proteins were denatured for 5 min at 95 °C and cooled to RT before acrylamide (#1610140, Bio-Rad) was added to a final concentration of 4% (w/v ...
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Emergence of RNA-guided transcription factors via domestication of transposon-encoded TnpB nucleasesbioRxiv - Genetics 2023Quote: ... Quantitative PCR was performed in 10 µl reaction containing 5 µl SsoAdvanced™ Universal SYBR Green Supermix (BioRad), 1 µl H20 ...
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bioRxiv - Physiology 2024Quote: ... Membranes were blocked with Tris-buffered saline with 0.05% Tween-20 (TBST) containing 5% dried nonfat milk (Biorad). Primary antibodies were diluted in TBS-T containing 5% dry nonfat milk (Biorad) ...
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bioRxiv - Microbiology 2022Quote: ... Membrane was washed 3x for 5 min with TBS-T and developed with Clarity Western ECL (Bio-Rad) per manufacturers guidelines ...
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bioRxiv - Immunology 2022Quote: ... 42 °C for 50 min and 85 °C for 5 min in a thermal cycler (Bio-Rad, USA). Synthesized cDNA was kept at −80 °C till further use ...
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bioRxiv - Plant Biology 2022Quote: ... The DNA was resuspended with Tris-EDTA and 5% Chelex-100 chelating resin (Bio-Rad Laboratories, Hercules, CA).
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bioRxiv - Microbiology 2022Quote: Each 10 μl PCR reaction consisted of 5 μl of SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, Germany), 250 nM of forward and reverse primers ...
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bioRxiv - Cell Biology 2022Quote: ... Total protein (5-10 ug) was loaded in 15 well pre-casted gradient gel (456-8086, Bio-Rad). After electrophoresis ...
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bioRxiv - Cell Biology 2023Quote: ... 20 μg of protein extracts were loaded and run on 5-20% gradient pre-cast gels (Bio-Rad) before being transferred onto a 0.45 μM nitrocellulose membrane (Amersham ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked with Tris-buffered saline with 0.05% Tween-20 (TBST) containing 5% dried nonfat milk (Biorad). Primary antibodies were diluted in TBST containing 5% bovine serum albumin and 0.02% NaN3 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were stained with 5 μM MitoSOX for 20 minutes and analyzed using ZE5 Cell Analyzer (Bio-rad).
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bioRxiv - Immunology 2023Quote: ... Lysates were heated at 95 °C for 5 min together with Laemmli sample buffer (Bio-Rad, catalog #1610747). Lysates were resolved using a 4% to 15% Criterion TGX Stain-Free Precast gel (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... according to the manufacturer’s instructions and an iQ™5 real-time PCR System (Bio-Rad Laboratories, USA). All reactions were pre-denaturation at 95°C for 3 min ...
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bioRxiv - Developmental Biology 2023Quote: ... for 5 minutes each and then blocked for one hour in 0.5% Blotting-Grade Blocker (Bio-Rad, 1706404) diluted in TBST at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... Real-time quantitative PCR was performed using the MyiQ™5 Real-Time PCR Detection System (Bio-Rad) using the following thermal cycle parameters ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was diluted 5-fold and qRT-PCR was performed using iTaq Universal SYBR Green Supermix (Bio-Rad) in CFX Connect Real-time System (Bio-Rad) ...
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bioRxiv - Immunology 2024Quote: ... 5 ng of cDNA was then subjected to qPCR analysis using iQ SYBR Green super-mix reagent (BioRad). Gene expression levels were normalized to a housekeeping gene (TATA-binding protein or HPRT) ...
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bioRxiv - Physiology 2024Quote: ... Protein (5-10 µg) was separated by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) (Bio-Rad, USA) in 26-well 4–15% Criterion™ TGX Stain-Free™ Protein Gels (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... The formazan crystals formed by the cells were dissolved by adding 100 µL of 5% SDS (Bio-Rad) in 1N HCl (Thermo Fisher ...
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bioRxiv - Biochemistry 2024Quote: ... Integrity of the annealed oligonucleotides was determined by application to Mini-PROTEAN native 5% polyacrylamide TBE gel (Biorad), staining with SYBR safe DNA gel stain (Thermo Fischer Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were blocked at room temperature for 1 hour in 5% nonfat dry milk (blocking grade, Bio-Rad) dissolved in Tris-buffered saline containing 0.1% Tween 20 (TBST) ...
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bioRxiv - Biochemistry 2024Quote: ... Pooled peak fractions were then loaded onto a 5 ml Bio-Scale Mini CHT Type I Cartridge (Biorad) in 10 mM NaH2PO4 Buffer and eluted sequentially with 2CV 40 mM NaH2PO4 Buffer ...
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bioRxiv - Genetics 2024Quote: ... Electroporation was performed at 2.5 kV for 5 ms in a MicroPulser electroporator (Bio-Rad, Hercules, California, USA) using 0.2 cm cuvettes ...
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bioRxiv - Cancer Biology 2024Quote: ... at 95 degrees Celsius for 5 minutes before loading onto 10% Mini-PROTEAN TGX precast protein gel (BioRad). PageRuler Plus Pre-stained Protein Ladder (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... and 5 µg total protein was loaded on each lane of a Mini Protean TGX Gel (BioRad, #4561093) together with a standard (WesternC ...
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bioRxiv - Biochemistry 2024Quote: ... boiled for 5 min and then resolved on 4-20% (w/v) gradient SDS-PAGE gels (Bio-Rad) with Tris-glycine buffer ...
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bioRxiv - Cell Biology 2020Quote: ... destained for 2 h at room temp (Coomassie Brilliant Blue R-250 staining kit, BIO-RAD), and imaged on a ChemiDoc MP (BIO-RAD).
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 specific genome levels were measured by qPCR using SsoFast EvaGreen supermix (Bio-Rad) according to manufacturer’s protocol.
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bioRxiv - Biochemistry 2020Quote: ... Biotinylated small RNAs were separated from HPDP-biotin and pyridine-2-thione using spin columns (BioRad) in ultra-pure water.
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bioRxiv - Developmental Biology 2020Quote: ... We ran the qPCR reactions using 1-2 µl cDNA in SYBR Green Master Mix (Biorad) totaling 20 µl in a Biorad CFX96 machine using 60°C as the annealing temperature ...
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bioRxiv - Bioengineering 2021Quote: ... and the selected primers (Table 2) using a CFX Connect™ Real-Time System (Bio-Rad) under the following conditions ...
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bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies were used: anti–α-tubulin (MCA77G YL1/2, rat monoclonal; Bio-Rad), anti-GAPDH (mouse ...
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bioRxiv - Cell Biology 2021Quote: Samples for electrophoresis were prepared by mixing with 2× Laemmli sample buffer (Bio-rad, Hercules, California) and denatured at 55°C for 5 min ...
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bioRxiv - Cell Biology 2021Quote: ... 2-3 μg of total protein were loaded onto 10% SDS-polyacrylamide (29:1 Bio-Rad) gels with 10 μM phostag reagent (FUJIFILM Wako Chemicals ...