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Citations for Bio-Rad :
1201 - 1250 of 2255 citations for Rabbit Anti Human IgG gamma chain Alexa Fluor 405 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... an assay detecting the human ribonuclease P/MRP subunit p30 RPP30 gene (Bio-Rad Assay ID dHsaCP2500350) was used as reference (probe ...
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bioRxiv - Cell Biology 2022Quote: One segment of each explanted human IPF lung was filled with 1.5% Low Melt Agarose (Bio-Rad) at 37°C and allowed to cool on ice for 30 mins ...
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bioRxiv - Cancer Biology 2021Quote: ... For the quantification of metabolic hormones Bio-Plex Pro Human Diabetes 10-plex Assay (BioRad, USA; 171A7001M) was used ...
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bioRxiv - Immunology 2019Quote: ... cells were incubated at 370C for a further 24 h with human oxLDL (#5685-3557, Bio-Rad) at a concentration of 0 µg/ml and 25µg/ml ...
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bioRxiv - Cell Biology 2019Quote: One segment of each explanted human IPF lung was filled with 1.5% Low Melt Agarose (Bio-Rad) at 37°C and allowed to cool on ice for 30 min ...
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bioRxiv - Immunology 2021Quote: ... IL-18 and IL-6 concentrations were measured using the Bio-Plex Pro Human Cytokine Assay (BioRad).
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bioRxiv - Immunology 2022Quote: ... Cytokines and chemokines were measured using the Bio-Plex Pro™ Human Assay (Bio-Rad, Hercules, USA): cytokine screening panel plus ICAM-1 and VCAM-1 (12007283 ...
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bioRxiv - Pathology 2024Quote: ... and cytokines were detected using the Bio-Plex Pro Human Cytokine 17-plex Assay (Bio-Rad, #M5000031YV), including IL-1β ...
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bioRxiv - Cancer Biology 2024Quote: ... diluted 1:4 and analyzed using Bio-Plex ProTM Human Chemokine Panel 40-plex Assay (Bio-rad). Acquisitions and analyses were performed on a Bio-Plex 200 system with Manager 6.1 Software (Bio-rad) ...
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bioRxiv - Immunology 2023Quote: ... Human AAVS constructs were transfected into CHO cells using the Gene Pulser MXcell Electroporation System (Bio-Rad). Transfected cells were selected for with 10µg/mL puromycin or 1mg/mL neomycin ...
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bioRxiv - Molecular Biology 2021Quote: ... mouse-HRP and rabbit-HRP secondary antibodies were purchased from BioRad Laboratories (Hercules ...
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bioRxiv - Microbiology 2020Quote: ... probed with goat α-rabbit HRP-conjugated secondary antibody (Bio-Rad) at a 1:2000 dilution in TBS-T for 45 min at room temperature with shaking ...
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bioRxiv - Microbiology 2023Quote: ... pneumoniae strains previously incubated in 25% of baby rabbit complement (BioRad) at 37°C for 30 mins were added to fresh human neutrophils extracted from blood from healthy volunteers using MACSxpress kit (Miltenyi Biotec ...
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bioRxiv - Microbiology 2023Quote: ... HRP-labeled α-rabbit secondary 1:10,000 (BioRAD Catalog #170-6515); and/or HRP-labeled α-mouse secondary (Cell Signaling Technology Catalog #7076S) ...
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bioRxiv - Microbiology 2024Quote: ... HRP-labeled α-rabbit secondary 1:10,000 (BioRAD Catalog #170-6515); HRP-labeled α-mouse secondary (Cell Signaling Technology Catalog #7076S) ...
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bioRxiv - Microbiology 2024Quote: ... probed with goat α-rabbit HRP-conjugated secondary antibody (Bio-Rad) at a 1:2000 dilution in TBS-T for 45 min at room temperature with shaking ...
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bioRxiv - Cell Biology 2024Quote: ... StarBright Blue 700 mouse and rabbit (Bio-Rad, #12004158 and #12004161), Peroxidase AffiniPure™ Goat Anti-Mouse IgG (H+L ...
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bioRxiv - Pathology 2019Quote: ... Bands corresponding to EPCR and the housekeeping protein β-actin (mouse IgG #NB600-501, Novus, at 1:40,000) were detected by the chemiluminescence method (Clarity Western ECL Bio-Rad) and finally densitometrically measured by ImageJ 1.6.0 software.
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bioRxiv - Cell Biology 2019Quote: ... The secondary antibodies used for Western blotting were goat ⍰ -mouse IgG HRP conjugate (170-6516 from Bio-Rad; 1:2000) and goat ⍰ -rabbit IgG HRP conjugate (170-6515 from Bio-Rad ...
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bioRxiv - Plant Biology 2020Quote: ... was used at a dilution of 1:2,000 and it was visualized by incubation with goat anti-mouse IgG secondary antibodies conjugated to horseradish peroxidase (1:2,000) followed by a chemiluminescence reaction (Clarity Western ECL Substrate; Bio-rad). For the SS-TOM lines analysis ...
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bioRxiv - Cell Biology 2020Quote: ... Goat α-mouse secondary IgG-HRP antibody was used at a 1:5,000 dilution (BioRad Laboratories, Hercules, CA, 170-6516). Phospho-MAPK antibodies were incubated in 1X TBST (10 mM TRIS-HCl pH 8 ...
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bioRxiv - Microbiology 2019Quote: ... The lysate was used to bind to 200ul of IgG Sepharose beads (Amersham Biosciences) for 2h at 4°C using a disposable chromatography column (BioRad). The IgG-Sepharose column was washed with 35 ml of protein A binding buffer (10 mM Tris–HCl ...
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bioRxiv - Bioengineering 2024Quote: ... Each column was calibrated using 660 kDa to 1.35 kDa molecular weight protein standards (thyroglobulin, IgG, ovalbumin, myoglobin, and vitamin B12) (Bio-Rad). Following peak analysis by SDS-PAGE ...
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bioRxiv - Biochemistry 2023Quote: ... and Mouse IgG HRP-linked whole Ab (Cytiva), then imaged using (ECL Select, Cytiva) with a ChemiDoc MP (Bio-Rad). For gel quantification ...
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bioRxiv - Microbiology 2023Quote: ... followed by immunoprecipitation with 5 µg appropriate antibodies or corresponding IgG control using magnetic protein A/G beads (BIO-RAD). After several rounds of washing with low-salt wash buffer (20 mM Tris-HCl ...
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bioRxiv - Microbiology 2020Quote: ... Analyses were performed with the Extended Bio-Plex Pro Human Cytokine 48-Plex Screening Panel (Biorad, Munich, Germany) as described previously 72 and using a 2-laser reader allowing simultaneous quantification of cytokines and chemokines ...
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bioRxiv - Immunology 2019Quote: ... fatty acid free BSA Efflux was measured after a 4-hour incubation period with 0µg/ml and 50µg/ml of human HDL (#5685-2004, BioRad). Supernatants were collected and the cells lysed with 1% (w/v ...
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bioRxiv - Neuroscience 2023Quote: ... and Bio-Plex Pro Human Inflammation Panel 1 (37-plex) (Cat. No. 171AL001M) from Bio-Rad (Hercules, CA).
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bioRxiv - Neuroscience 2021Quote: ... followed by secondary peroxidase-conjugated antibodies produced in rabbit (1:10,000; Biorad). Immunoreactivity was detected by enhanced chemiluminescence (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... HRP-conjugated antibodies against mouse and rabbit were purchased from Bio-Rad Laboratories (1:5000)
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bioRxiv - Genomics 2024Quote: ... anti-Pk (BioRad), anti-Pol II antibody CTD4H8 (Abcam) ...
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bioRxiv - Cell Biology 2021Quote: ... the membranes were washed 3 times for 5 min and incubated with the secondary antibodies (1:10000, IgG-HRP, Bio-Rad) for 45 min at RT ...
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bioRxiv - Immunology 2022Quote: ... and RBD-specified single memory B cells were gated by IgG+RBD+ followed by single-cell sorting into 96-well PCR plates (free of DNase and RNase, Bio-Rad) containing 10μl lysis buffer (1X reverse transcriptase reaction buffer containing RNAse inhibitor) ...
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bioRxiv - Biochemistry 2022Quote: ... Input and MOV10/IgG IP-ed samples were resolved on a 4–12% Criterion XT Bis-Tris Protein Gel (Cat. No. 3450125, Bio-Rad) and analysed through Western blot using specific antibodies.
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bioRxiv - Biochemistry 2022Quote: ... MOV10/IgG IP-ed samples were eluted from the beads in 30 µL of XT buffer (Cat. No. 1610791, Bio-Rad) through heating at 95 °C for 10 min ...
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bioRxiv - Neuroscience 2019Quote: ... The concentration of IgG present after dialysis was determined using a modified Lowry assay (DC protein assay, BioRad, Hemel Hempstead, UK) and adjusted by dilution with Hartmann’s or by dialysis against a sucrose solution (Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2019Quote: ... was used at a dilution of 1:1.000 and it was visualized by incubation with goat anti-mouse IgG secondary antibodies conjugated to horseradish peroxidase (1:10.000) followed by a chemiluminescence reaction (Clarity ™ Western ECL Substrate, BIO-RAD).
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bioRxiv - Plant Biology 2021Quote: ... membranes were incubated with HRP-conjugated secondary antibodies (rat (IgM)/LM20 and LM19 mouse (IgG)/2F4 (in 5% milk PBS) and after washing chemiluminescence induced with Clarity Western ECL Substrate (Biorad, #1705061) and detected with ChemiDoc imaging system (Biorad).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... were then generated using the piggyBac transposon system as described.12 Protein expression was induced with doxycycline and secreted fusion protein was separated from expression media using IgG Sepharose 6 Fast Flow resin (GE-Healthcare) in a 10 mL Poly-Prep chromatography column (Bio-Rad). Resin was washed with 50 column volumes of wash buffer (10 mM Tris pH 7.5 ...
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bioRxiv - Synthetic Biology 2020Quote: ... 560 µl of FBP-sepharose were mixed with 8.4 mL of 20 nM CAL Fluor Red 610-labelled RNAs in a Econo-Column Chromatography Column (BioRad, Hercules,CA), and the column washed with CB until no fluorescence change was detected in the flow-through ...
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bioRxiv - Biochemistry 2021Quote: ... followed by in-gel fluorescence scanning of Alexa 647 (ChemiDoc™ MP device, Bio-Rad Laboratories GmbH, München, Germany) and silver staining (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: Cytokine and growth factor secretion was measured with the Bio-Plex Pro Human Cytokine Screening Panel (Bio-Rad #M500KCAF0Y), a 27-plex assay ...
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bioRxiv - Neuroscience 2021Quote: ... The isolated placental CD34+ single cell suspension was incubated with human CD34- phycoerythrin (PE) (Bio-Rad; dilution 1:25), human CD45-FITC (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... Mono-Mac-6 effector cells previously stimulated with recombinant human IFN-ɣ (Bio-Rad, Hercules, CA, USA; cat. PHP050) at 0.2 µg ml-1 for 3h at 37 °C ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The cytokine levels were measured using the magnetic bead-based Bio-Plex Pro Human cytokine 27-Plex (Bio-Rad) according to manufacturer’s instructions and measured the cytokines using the Bio-Plex 200 instrument ...
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bioRxiv - Microbiology 2023Quote: ... The samples were analyzed in duplicates using the Bio-Plex Pro™ Human Cytokine panel (Bio-Rad, Hercules, CA), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: Cytokines were quantified in media isolated from MN cultures using the Bio-Plex Pro Human Cytokine kit (Biorad, USA) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... anti-infliximab (HCA213G) and anti-adalimumab (HCA205) were ordered from BioRad. Similarly ...
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bioRxiv - Immunology 2024Quote: ... Anti-F4/80 (rat anti-mouse MCA497A647, Biorad, dilution 1:500) and anti-Parp14 (sc377150 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... A solution of rabbit αFITC antibody (1 μg/mL, Bio-Rad 4510-7804) in 2% Milk-PBS was added to each well (100 μL/well ...