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Citations for Bio-Rad :
1201 - 1250 of 1382 citations for 6 FLUORO 2 TRIFLUOROMETHYLQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... The PCR products were run on a 2% agarose gel and images were taken on a Chemidoc MP imaging system (BIO-RAD). Image J was used to analyze the pixel intensity of 3R (288 bp ...
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bioRxiv - Biochemistry 2023Quote: ... for 2 h (for the ERGIC-53 ΔΗ34 mutant) The beads were transferred into a gravity flow column (Econo-column, Bio-Rad) and washed with 20 CV of buffer A supplemented with 0.02% (w/v ...
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bioRxiv - Cancer Biology 2023Quote: ... Sodium-dodecyl-sulfate polyacrylamide gel electrophoresis and transfers of proteins to .45uM nitrocellulose membranes were performed using the Protean 2 or 3 systems (Bio-Rad) and protocols provided by the manufacturer ...
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bioRxiv - Immunology 2023Quote: ... was used for positive signals and the chemiluminescence was detected using a Light Capture AE-6971/2 device (ATTO) or ChemiDoc Touch Imaging System (Bio-Rad).
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bioRxiv - Cell Biology 2023Quote: ... before being re-incubated with the secondary antibodies at room temperatures for 2 h and then subjected to the imaging of immunoblots by Bio-Rad instrument ...
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bioRxiv - Cell Biology 2023Quote: ... Protein content was confirmed by mixing 2 μL of each elution step with 200 μL Bradford solution (Protein assay dye reagent (Bio-Rad) diluted 1:5 in H2O) ...
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bioRxiv - Genomics 2023Quote: ... 10ul of PCR product of each replicate for each gene was loaded in separate wells in 2% agarose gels and imaged using a ChemiDocTM MP Imaging system (BioRad, USA). All replicates for each gene were loaded on the same gel ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were resuspended in 2 ml of 10% sorbitol and 40 μl per transformation mixed with up to 5 μl of plasmids were electroporated in 2 mm cuvettes (Gene Pulser Xcell BIO-RAD). For autoactivation controls ...
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bioRxiv - Biochemistry 2022Quote: An equivalent volume of ∼150 µg FBS was first diluted 1:2 with loading buffer (consisting of 2X Laemmli Sample Buffer (#1610737, Bio-Rad) plus a 5% (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... 2 μg of RNA template was converted to cDNA using iScript™ cDNA Synthesis Kit (1708891, Bio-Rad, Hercules, CA, USA), according to the manufactures’ instructions ...
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bioRxiv - Microbiology 2023Quote: ... The reaction mixtures contained 100 ng RNA template per sample diluted 2-fold with qPCR solution (iTaq Universal SYBR Green One-Step Kit, Bio-Rad) containing the primers D2S and D2C ...
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bioRxiv - Biochemistry 2023Quote: ... in a 100 μL reaction for 2 hours at 37°C and purified using Micro-Bio P-30 spin columns (Bio-Rad). The radiolabeled probe was heated to 90°C for 2 minutes and resuspended in 10 mL of ULTRAhyb Oligo hybridization buffer (Thermo Fisher) ...
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bioRxiv - Microbiology 2023Quote: ... This panel had been tested negative for SARS-CoV-2 nucleocapsid protein expression using Bio-Plex Pro Human IgG SARS-CoV-2 N/RBD/S1/S2 4-Plex Panel (Bio-rad). The second panel consisted of 20 sera from individuals who were previously infected by SARS-CoV-2 vaccinated with 2-4 doses of parental mRNA vaccine ...
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bioRxiv - Plant Biology 2023Quote: ... Reverse transcription reaction was conducted using 0.5 µg of total RNA and 2 µl of iScript™ Reverse Transcription Supermix (Bio-Rad) in 10 µl reaction volume ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Specific antigens were detected after incubating 2 h at room temperature goat anti-rabbit IgG horseradish peroxidase conjugate (BioRad Laboratories, Inc.). Immunoreactivity was visualized by electro-chemiluminescence in a Chemi-Doc MP Imaging System (Bio Rad).
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bioRxiv - Cancer Biology 2023Quote: ... Reverse transcription (RT) was performed from 2 to 5 µg of RNA with iScript Adv cDNA synthesis kit (Bio-Rad #1725038). The cDNA was diluted 5 times and 1 μL of this was used for each reaction in real-time PCR ...
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bioRxiv - Cancer Biology 2023Quote: ... 30 μg of each sample was boiled with 4x Laemmli Sample Buffer containing 10% 2-mercaptoethanol (#1610747, BioRad, Hercules, CA, USA) and run on a Mini-PROTEAN® TGX™ Precast Gel (#4561083 ...
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bioRxiv - Cell Biology 2023Quote: ... followed by washes at room temperature (1 x 15 min, 2 x 5 min) and incubation with HRP-conjugated secondary antibodies (Bio-Rad) for 1 h ...
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bioRxiv - Cancer Biology 2023Quote: ... before solubilisation in 2% SDS (Severn Biotech, Worcs, UK) and subsequent OD595 measurements were obtained using an iMark microplate reader (Bio-Rad). Data were normalised by dividing raw OCR/ECAR measurement by OD595 for each well of the culture plate ...
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bioRxiv - Biochemistry 2023Quote: ... This mixture was then incubated at 4°C for 2 h with gentle agitation before reconstitution was initiated by removing detergent by incubating with Bio-beads (Bio-Rad) at 4°C overnight with constant rotation ...
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bioRxiv - Molecular Biology 2023Quote: ... Reaction products were visualized on a 2 % agarose gel with 0.05% (v/v) Ethidium Bromide and visualized with a Gel Doc XR+ imager (Bio-Rad Laboratories). PCR amplicons were column purified with the Monarch® PCR & DNA Cleanup Kit and eluted in 25 µL of Monarch® DNA Elution Buffer.
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bioRxiv - Biophysics 2024Quote: ... then diluted below the CMC of C12E8 with reconstitution buffer and added to 0.5 g methanol-activated Biobeads SM-2 (Bio-Rad; 1523920). The mixture was rotated end over end for 18 hours and then subject to room temperature size exclusion chromatography on a Superose 6 10/300 GL column ...
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bioRxiv - Neuroscience 2024Quote: ... Viability of magnetically-captured cells (eluted into a new tube away from the magnet) was assessed by trypan blue staining (1:2 dilution sample in trypan blue; BioRad 1450013). Cell viability was quantified as the proportion of cells that did not take up the dye ...
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bioRxiv - Genetics 2024Quote: ... Membranes were were in contact with the luminol-H2O2 solution for 2 minutes and then exposed for imaging using the Chemidoc imaging system (Bio-Rad).
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bioRxiv - Cell Biology 2024Quote: ... clarified at 20,000 g for 2 minutes and 15 µL was loaded onto a 4-20% tris-glycine SDS-PAGE gel (Biorad #5671094). Proteins were then transferred to nitrocellulose (Sigma #10600001 ...
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bioRxiv - Microbiology 2024Quote: ... 10 µg Capped mRNA transcripts were electroporated into BHK-21 cells in a 0.2 cm cuvette (25 μF, 1.5 kV, and 200 Ω) with the help of Gene Pulser Xcell Electroporation system (Bio-Rad) and resuspended in antibiotic-free DMEM supplemented with 10% FBS ...
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bioRxiv - Microbiology 2019Quote: ... Each PCR was performed in a total volume of 10 µL containing 5 µL of 2 × iQSYBR Green supermix (Bio-Rad, USA), 200 nM of each primer ...
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bioRxiv - Molecular Biology 2021Quote: Amplification was carried out using PCR mixture-2 blue (AmpliSens, Russia) containing Taq-polymerase on a T100 Thermal Cycler (Bio-Rad, USA). Next ...
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bioRxiv - Molecular Biology 2021Quote: ... and 2% β-mercaptoethanol) and separated on a 10% gel by SDS-PAGE (TGX Stain-Free™ FastCast™ Acrylamide Solutions; BIORAD) or 4-15% gel (Mini-PROTEAN® TGX Stain-Free™ ...
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bioRxiv - Genomics 2022Quote: ... Each sample was tested by PCR systems (threshold – 0,050) to the presence of SARS-CoV-2 using a CFX Connect Real-Time PCR System (BioRad, Hercules, California, USA). RT-PCR were implemented in 40 μl of final volumes ...
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bioRxiv - Neuroscience 2019Quote: The membrane was washed in 1x Tris buffered saline with 0.1% Tween-20 (TBS-T) and blocked in TBS-T with 2% (w/v) blotting grade blocker (Bio-Rad, Cat#: 1706404). Membranes were incubated in primary antibodies (Table 2 ...
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bioRxiv - Biochemistry 2021Quote: ... the reactant was mixed with the 2 × SDS-PAGE sample buffer containing 100 mM β-mercaptoethanol and run on 12% Mini-PROTEAN TGX Precast Gel (Bio-Rad) without a boiling step ...
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bioRxiv - Microbiology 2021Quote: ... The amplified genomic DNA from the PPA strains was resolved using 2% agarose gel and visualized in the Gel DocTM XR+ documentation system (Bio-Rad, USA).
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bioRxiv - Microbiology 2021Quote: ... aeruginosa isolates were separated in 2% agarose gel and visualized their banding patterns in the Gel DocTM XR+ documentation system (Bio-Rad, USA).
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bioRxiv - Neuroscience 2021Quote: ... Samples were then loaded onto 4-15% Criterion™ TGX™ Precast Midi Protein Gels (12 + 2 wells, 45 μl) (Bio-Rad) and subjected to SDS-PAGE at 200 V for ~45 min in 10× Tris/Glycine/SDS running buffer (Bio-Rad) ...
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bioRxiv - Neuroscience 2021Quote: ... Master mixes containing primer pairs against FIBCD1 or B2M (Beta-2-microglobulin, housekeeping gene) were prepared with iTaq Universal SYBR Green Supermix (Bio-Rad #1725122). The mastermixes were dispensed into each well containing cDNA and incubated on ice for 15 minutes to allow the cDNA to dissolve ...
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bioRxiv - Plant Biology 2020Quote: ... Reactions were performed with 2 × Taq Master Mix (Vazyme, Nanjing, China) on a Bio-Rad Thermal Cycler (Bio-Rad, Hercules, CA, USA) using the following procedure ...
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bioRxiv - Genomics 2021Quote: ... PCR products were resolved using a 2% agarose tris-acetate-EDTA gel and a 177 bp band was visualized using the ChemiDoc™ imaging system (Bio-Rad).
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bioRxiv - Microbiology 2021Quote: ... 100 ng plasmid DNA and 100 μL of electrocompetent mycobacteria were mixed and transferred to a 2 mm electroporation cuvette (Bio-Rad #1652082). Where necessary ...
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bioRxiv - Neuroscience 2020Quote: ... and followed by incubation with HRP-conjugated secondary antibodies for 2 hours at room temperature: goat anti-rabbit IgG (BioRad, #170-6515) or goat antimouse IgG (BioRad ...
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bioRxiv - Plant Biology 2020Quote: ... or 1 μl (for the rest) of a 1/2 dilution of the cDNA was added to a reaction containing 1X of SsoFast EvaGreen (Bio-Rad, USA), 0.5 μM Forward and Reverse primers (Table 2) ...
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bioRxiv - Microbiology 2020Quote: ... which subsequently performed 2-DE using a 7 cm immobilized pH gradient (IPG) strips (pH range of 3—10, Ready strip, Bio-Rad, USA).
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bioRxiv - Cancer Biology 2021Quote: ... 60 of High Capacity NeutrAvidin slurry (Thermo PI29204) were washed three times with 1 mL 2X RIPA/1mM EDTA buffer in 2-mL chromatography columns (Bio-Rad 7326008) attached to a vacuum manifold (Promega A7231) ...
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bioRxiv - Cell Biology 2021Quote: ... Selected siRNAs were spotted onto a gridded MatTek dish (P35G-2-14-C-GRID) using a contact spotter (ChipWriter Pro-Bio-Rad Laboratories) resulting in a layout of 4 × 8 spots 40 ...
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bioRxiv - Microbiology 2022Quote: SARS-CoV-2 viral RNA loads were determined using Droplet Digital PCR (ddPCR) supermix for probes without dUTP (Bio-Rad Laboratories Ltd) and the QX200 Droplet Digital PCR System Workflow (Bio-Rad Laboratories Ltd) ...
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bioRxiv - Neuroscience 2020Quote: Brain tissues were homogenized in lysis buffer and then combined with 2 x Laemelli buffer (Bio-Rad Laboratories, Inc., Hercules, CA, USA). Protein samples were then separated on 7.5 to 12.5% v/v sodium dodecyl sulfate (SDS ...
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bioRxiv - Developmental Biology 2021Quote: ... following by 25 cycles of preamplification using mix of 47 pairs for MTE primers, each at final 50nM concentration (Supplementary information, Supplementary Table 2) and SsoAdvanced™ PreAmp Supermix (Cat# 1725160, Bio-Rad). After amplification ...
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bioRxiv - Biochemistry 2021Quote: ... The purified proteins were running in 2-D gel electrophoresis and images were taken and analyzed in gel dock(Bio-Rad, USA). The details of protein purification protocols like TCA/Acetone ...
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bioRxiv - Plant Biology 2021Quote: ... The pelleted nuclei were re-suspended in 200 µl of NIB and mixed with a 140 µl aliquot of melted 2% LMA agarose (Bio-Rad, 1703594) at 43°C ...
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bioRxiv - Microbiology 2020Quote: ... Non-human primate samples were inactivated with γ-radiation (2 MRad) according to standard operating procedures and assayed on a Bio-Plex 200 instrument (Bio-Rad) using the Non-Human Primate Cytokine MILLIPLEX map 23-plex kit (Millipore ...