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Citations for Bio-Rad :
1101 - 1150 of 8850 citations for 2 5 Sulfanyl 4H 1 2 4 triazol 3 yl phenol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... Then 2 μl of 10 times diluted cDNA was used for CFX96 Touch Real-Time PCR Detection System (Bio-Rad) for Syber green based quantitative PCR analysis ...
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bioRxiv - Zoology 2022Quote: ... and folded by heating to 95°C for 2 min and slowly cooling down at 0.1 °C per second using a thermocycler (Bio-Rad). DsRNA concentrations were adjusted to 3 or 14 μg / μl using SpeedVac Concentrator (Thermo Scientific ...
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bioRxiv - Microbiology 2022Quote: ... The resulting cDNA was diluted 1:10 and 2 µL was used for qPCR amplification of SARS-CoV-2 spike using iTaq Universal SYBR Green Supermix according to the manufacturer’s protocol (Bio-Rad). Copy number was calculated in reference to a standard curve of known copy number (102 – 107 copies spike) ...
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bioRxiv - Synthetic Biology 2022Quote: ... the mixture was transferred to ice-cold electroporation cuvettes with a 2 mm gap (Bio-Rad Laboratories GmbH, Germany, #1652086). Electroporation was performed using the MicroPulser Electroporator (Bio-Rad Laboratories GmbH ...
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bioRxiv - Synthetic Biology 2023Quote: ... and incubated for 24 h in a thermal gradient using a DNA Engine Tetrad 2 Peltier Thermal Cycler (Bio-Rad) with the lid set to 50°C ...
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bioRxiv - Microbiology 2022Quote: ... competent cells were mixed with 500 ng of DNA in electroporation cuvettes (2 mm gap) and the reactions were electroporated (2.5 kV) using a MicroPulser electroporator (Bio-Rad). Electroporated cells were recovered in 1 mL LB (unless otherwise stated ...
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bioRxiv - Immunology 2024Quote: ... mixed with 25 μl 1.5 mM CaCl2 and 42 μl ddH2O using 2 mm gap cuvettes with 3.5 msec/500 volt in BIORAD Gene Pulser Xcell Electroporation System (BioRad, USA). Transfected cells were immediately transferred into 5 mL prewarmed medium in 25 cm2 flasks and left to recover overnight at 26 °C ...
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bioRxiv - Microbiology 2023Quote: ... cells and plasmids in 10% glycerol were first heat-shocked at 56°C for 2 min followed by immediate electroporation in 0.1-cm cuvette (Bio-Rad) using electroporation program of 2.5 kV and time constant of 2.3-2.5 ms on the Bio-Rad Micropulser ...
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bioRxiv - Microbiology 2024Quote: ... 0.2 μM of each primer and 2 μl of template cDNA on a CFX Connect Real-Time System (Bio-Rad). Samples and plasmid standards were amplified using the following protocol ...
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bioRxiv - Biophysics 2023Quote: ... and yeast cell growth was monitored by measuring the OD600 at 2 h increments in a microplate reader (Benchmark Plus, BioRad). The remainder of the 10 ml cultures was used to assess Pgp expression by Western blot analysis of microsomal membrane preparations using the monoclonal C219 anti-Pgp antibody.
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bioRxiv - Microbiology 2023Quote: ... PCR products were run on 2% TBE agarose gel and post-stained with ViSafe Red Gel Stain (Vivantis Technologies) and visualised using GelDoc (BioRad). The relative intensity of the bands was quantified using ImageJ ...
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bioRxiv - Genomics 2023Quote: ... Gels were run in 1X tris-acetate SDS running buffer for 2 hr and transferred to a 0.45 μm PVDF membrane using the Turbo Trans-Blot transfer system (Bio-Rad) for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... 1.0 µg purified plasmid DNA was added to 0.2 ml aliquot of the above-prepared fusobacterial cells in a pre-cooled corvette (0.1 cm electrode gap, Bio-Rad) and left on ice for 10 minutes ...
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bioRxiv - Biophysics 2022Quote: Detergent removal and generation of proteoliposomes was achieved using polystyrene beads (Bio-Beads in SM-2 Adsorbent Media, BioRad, Inc.). The protocol was adapted and refined from 20 ...
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bioRxiv - Systems Biology 2023Quote: ... and total protein abundance was normalized to 2 μg using the Detergentcompatible Protein Assay (Biorad, Hercules, CA, USA, Cat. # 5000111). 2 μg α-RelA antibody (Cell Signaling ...
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bioRxiv - Microbiology 2023Quote: ... 1.0 mg purified plasmid DNA was added to 0.2 ml aliquot of the above-prepared fusobacterial cells in a pre-cooled corvette (0.1 cm electrode gap, Bio-Rad) and left on ice for 10 minutes ...
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bioRxiv - Genomics 2022Quote: ... Proteins were transferred to a 0.2 µm or a low-fluorescence 0.45 µm polyvinylidene difluoride (PVDF) transfer membrane using a Trans-Blot Turbo (Bio-Rad). Following antibody incubation ...
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bioRxiv - Genomics 2023Quote: ... Gels were run in 1X tris-acetate SDS running buffer for 2 hour and transferred to a 0.45 μm PVDF membrane using the Turbo Trans-Blot transfer system (Bio-Rad) for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA was generated from ∼2 ng of total RNA from each sample using iScript Reverse Transcription Supermix for qRT-PCR (170-8891; BioRad) according to manufacturer’s protocols ...
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bioRxiv - Microbiology 2023Quote: ... 390 μl of cells were mixed with 10 μg of in vitro transcribed RNA and added to a 2 mm electroporation cuvette (BioRad). BHK-21 cells were electroporated with 1 pulse at 1.2 kV ...
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bioRxiv - Microbiology 2023Quote: ... 0.2 μM of each primer and 2 μl of template cDNA on a CFX Connect Real-Time System (Bio-Rad). Duplicates of samples and plasmid standards were amplified by using the following protocol ...
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bioRxiv - Cell Biology 2023Quote: ... then the pellet was resuspended in 212 µL of Laemmli sample buffer with 2-mercaptoethanol added (Bio-Rad, Hercules, CA). To neutralize the residual TCA ...
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bioRxiv - Microbiology 2023Quote: ... Relative expression was determined using the 2−ΔΔCT method129 as part of the CFX Maestro for Mac 1.0 software package (Bio-Rad).
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bioRxiv - Microbiology 2023Quote: ... The mixture was allowed to stand at room temperature in the dark for 30 min before the detergent was removed by successive addition of BioBeads SM-2 (BioRad). Nanodiscs were concentrated using Vivaspin® 500 centrifugal filters (10 kDa MWCO PES membrane).
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bioRxiv - Cell Biology 2023Quote: ... Cell pellets were resuspended by addition of ice-cold PBS supplemented with 2% FBS and counted using a TC-20 cell counter (BioRad). 2.5-5x105 cells were harvested ...
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bioRxiv - Cell Biology 2024Quote: ... 500μL of sample was dialyzed (20 kDa cutoff) in 250 mL Buffer A with 0.5 g Biobeads SM-2 (Bio-Rad) for 16-18 hours at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... The ddPCR reaction mixture consisted of 12.5 µl of a 2× ddPCR Supermix for Probes no dUTP (1863024; Bio-Rad), 1.25 µl of each 20× primer-probe mix (Table 2) ...
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bioRxiv - Immunology 2024Quote: ... incubated in Radiance ECL HRP substrate (Azure Biosystems) for 2 min and visualized using the chemiluminescent setting on a ChemiDoc XRS+ (Biorad).
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bioRxiv - Immunology 2024Quote: ... and dialyzed overnight at 4 °C into 4 L of Experimental Buffer (25 mM Tris, 100 mM NaCl, pH 7.4) with 2 g/L Chelex 100 resin (Bio-Rad) to chelate residual transition metal ions ...
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bioRxiv - Microbiology 2024Quote: ... qPCR was performed using 2 µL of genomic DNA in SsoAdvancedTM Universal SYBR® Green Supermix (Bio-Rad, 172-5271) and primers for a 529-base pair repetitive element of T ...
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bioRxiv - Molecular Biology 2024Quote: ... Gene-specific cDNA synthesis was carried out with 2 µg of mtRNA using the iScript Select cDNA Synthesis Kit (BioRad) and oligo 2278 (see Supplementary Table ST2) ...
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bioRxiv - Immunology 2024Quote: ... and incubated in Radiance ECL HRP substrate (Azure Biosystems) for 2 min before visualization using the chemiluminescent setting on the ChemiDoc XRS+ (Biorad).
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bioRxiv - Microbiology 2024Quote: ... The PCR reaction mixture (25 μL total) consisted of 12.5 μL of 2× SYBR Green PCR Master Mix (Bio-Rad), 300 nmol each of forward and reverse primers ...
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bioRxiv - Microbiology 2024Quote: ... The was filtered through a 0.45 μm filter and His-MSI-2 was purified with HisTrapTM FF column (Cytiva) using the Econo Gradient Pump (BIORAD) according to the manufacturer’s protocol and overnight dialysis ...
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bioRxiv - Microbiology 2024Quote: ... DD-HA-BFD2Δeif1.2 were subjected to electrophoresis using 10% polyacrylamide SDS-PAGE gels and transferred onto 0.45-µm nitrocellulose membranes (Bio-Rad) with wet/tank transfer system (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... and gene specific primers were used to amplify cDNA and products were resolved using a 2% agarose gel and imaged via the ChemiDoc Touch Imaging system (BioRad). Primer sequences for both qPCR and PCR can be found in Table 4 and Table 5 ...
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bioRxiv - Microbiology 2024Quote: ... qPCR was performed using 2 µL of genomic DNA in SsoAdvancedTM Universal SYBR® Green Supermix (Bio-Rad, 172-5271) and primers for a 529-base pair repetitive element of T ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were transferred on nitrocellulose membrane at 100V for 2 h using the Mini trans-blot cell (Bio-Rad, #1703930). Membranes were blocked with 5% skim milk diluted in PBST (PBS + 0.1% Tween 20 ...
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bioRxiv - Biochemistry 2024Quote: ... Size Exclusion chromatography was performed using a column packed with Bio-Gel P-2 fine resins (Bio-Rad, Hercules, CA). HPLC analysis of compounds was performed on a Shimadzu LC-20A with a Waters XBridge BEH ...
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bioRxiv - Developmental Biology 2024Quote: ... the membrane was stained with Clarity Western ECL Substrate and visualized after a 2-minute incubation using the ChemiDoc MP Imaging System (BioRad).
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bioRxiv - Evolutionary Biology 2024Quote: ... PCR products were mixed with TriTrack loading loaded on 2 % agarose gels containing 2 µg /100 ml Midori Green and PCR amplicons were visualised using a ChemiDoc XRS+ Molecular Imager (Biorad).
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bioRxiv - Cell Biology 2024Quote: ... the solution was boiled for 2 x 7 min at 98°C and filtered through a 0.2 pm cellulose acetate membrane (Bio-Rad) with a 96-well vacuum Bio-Dot Apparatus (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... Nonspecific binding was blocked for 1 hr at RT with 5% BLOTTO (Bio-Rad) in Tris-buffered saline with 0.1% Tween (TBST) ...
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bioRxiv - Neuroscience 2020Quote: ... After 1 hour blocking in 5% non-fat milk solution (Bio-Rad 170-6404) at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Lysates were boiled for 5 min in 1 x Laemmli Sample Buffer (Bio-Rad) containing 5% β-mercaptoethanol ...
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bioRxiv - Microbiology 2020Quote: ... boiled and electrophoresed on 12% vol vol−1 and 4% vol vol−1 acrylamide SDS-PAGE gels (Bio-Rad Laboratories, Canada) on MiniProtean® camera (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2021Quote: ... All slides were subsequently blocked with 4% BSA and 1% Triton X-100 in PBS and incubated with rat anti-CD68 (1:500; Bio-Rad) and mouse-anti-GFP conjugated to Alexa Fluor 488 (1:250 ...
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bioRxiv - Neuroscience 2024Quote: ... at 1-4 °C and 100 volts for 1 h using Criterion™ blotter with wire electrodes (BioRad, Cat#1704071, USA). Blots were rinsed with phosphate-buffered saline (PBS) ...
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bioRxiv - Neuroscience 2024Quote: ... Reverse transcription reactions were performed using 4 µL of iScript at room temperature with 1 µg-1 pg of RNA template (Bio-Rad) in a final volume of 20 μL ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were denatured for 5 min at 95°C and gel was run using a 4-20% Mini-PROTEAN ® TGX precast protein gels (Bio-Rad, No. 4561094). Next ...