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Citations for Bio-Rad :
1001 - 1039 of 1039 citations for glass bottom plate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and cDNA template were loaded onto a 384-well plate and run through 40 cycles on a CFX384 real time cycler (Bio-Rad Laboratories, Inc). The data were analyzed using the manufacturer’s CFX manager software ...
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bioRxiv - Microbiology 2023Quote: ... PCR amplification reactions were conducted in 96-well optical plates on a Bio-Rad CFX Connect using 2x iTaq Universal SYBR Green Supermix (Bio-Rad, California, USA), 0.25 µM of each primer ...
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bioRxiv - Immunology 2022Quote: ... and 1 µl of respective primer pairs (200 nM final concentration) were mixed and distributed into 96-well qPCR plate (Bio-Rad; cat. #12001925). Droplets were generated in an Automated Droplet Generator (Bio-Rad) ...
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bioRxiv - Synthetic Biology 2022Quote: ... the plates were analyzed for luminescence signals using a ChemiDoc Imaging System and Image Lab 6.0.1 software (Bio-Rad Laboratories Inc., Hercules, USA). Exposure time was set to 5 sec ...
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bioRxiv - Immunology 2024Quote: ... The plate was analyzed on LUMINEX 200 (Luminex Corporation, Austin, TX, USA) and Bio-Plex Manager 6.1 (Bio-Rad Laboratories, Hercules, CA, USA).
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bioRxiv - Developmental Biology 2024Quote: ... and cDNA template were loaded onto a 384-well plate and run through 40 cycles on a CFX384 real time cycler (Bio-Rad Laboratories, Inc). The data were analyzed using the manufacturer’s CFX manager software ...
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bioRxiv - Biochemistry 2024Quote: ... and the plates were read at 415 nm using a Bio-Rad iMark™ Microplate Absorbance Reader (Bio-Rad Laboratories, Hercules, CA, USA).
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bioRxiv - Bioengineering 2021Quote: ... The plate was sealed with a pierceable seal (4titude; Wotton, UK) for 3 s at 180°C by using a plate sealer (BioRad PX-1; CA, USA) and maintained at 4°C during the LC-MS measurement ...
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bioRxiv - Biochemistry 2022Quote: ... 10μL of each time point were mixed with RNA loading dye and heated to 95°C before loading on a 1.0 cm well PAGE casting plate (Bio-Rad Laboratories, Mississauga, ON, Canada). Samples were run on a 7.5% Urea PAGE gel for 30 minutes at 300V in 0.5X TBE buffer ...
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bioRxiv - Plant Biology 2021Quote: ... were placed on 0.8% water-agar plates and transformed using biolistic bombardment with the PDS-1000/He™ system (Bio-Rad, Hercules, California, USA) as described above ...
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bioRxiv - Plant Biology 2021Quote: ... three primary leaves of 7 d old wildtype barley plants (cultivar Golden Promise) were placed on 0.8% water-agar plates and transformed using the PDS-1000/He™ system (Bio-Rad, Hercules, California, USA). For one transformation reaction ...
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bioRxiv - Biochemistry 2020Quote: DSF measurements were performed on a Bio-Rad CFX96 Touch Real-Time PCR Detection System in 96-well plates (Bio-Rad Hard-Shell, #HSP9601) sealed with transparent adhesive film (Bio-Rad Microseal ‘B’ Adhessive Sealer ...
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bioRxiv - Cancer Biology 2021Quote: ... XP2OS cells were transfected with 200 ng of plasmid containing the XPA VUS of interest or wild-type XPA as well as the FM-HCR reporter plasmids using the Gene Pulser MXCell Plate Electroporation System (Bio-Rad Laboratories #165-2670). Plate electroporation was performed at 260 V ...
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bioRxiv - Bioengineering 2023Quote: ... The plate was sealed with a pierceable seal (4titude; Wotton, UK) for 3 seconds at 180°C by using a plate sealer (BioRad PX-1; CA, USA) and maintained at 10°C during the LC-MS measurements ...
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bioRxiv - Biochemistry 2023Quote: ... Amplification and detection were performed in 96-well PCR plates using CFX connect Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules, CA, USA) in technical duplicate using the following protocol ...
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bioRxiv - Biophysics 2023Quote: ... Formazan crystals formed after 3 h in each well were dissolved in 150 μL of DMSO and the plates were read immediately in a microplate reader (BIO-RAD microplate reader-550) at 570 nm ...
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bioRxiv - Molecular Biology 2024Quote: qPCR was performed on a Bio-Rad CFX96 apparatus in Hard-Shell® 96-well PCR low-profile plates (Bio-Rad, cat. no: HSP9601) with Optical Microseal ‘B’ PCR Plate Sealing Film (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... United States) with adjusted concentrations of divalent cations Ca2+ and Mg2+ or on Mueller-Hinton Agar (MHA) plates (Bio-Rad, Marnes-la-Coquette, France). In conjugation experiments ...
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bioRxiv - Bioengineering 2023Quote: ... The plate was sealed with a pierceable seal (4titude; Wotton, UK) for 3 s at 180 °C using a plate sealer (PX-1; Bio-Rad, Hercules, CA, USA) and maintained at 10 °C during the LC-MS measurements ...
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bioRxiv - Cell Biology 2022Quote: ... Transcript analysis by qRT-PCR were performed using 2× ChamQ Universal SYBR qPCR Master Mix (Viazyme Biotech Co., Ltd., Nanjing, China) and a CFX96-C1000 96-well plate thermocycler (Bio-Rad, Hercules, CA, USA), the 18S rRNA was used as a reference gene25 ...
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bioRxiv - Biochemistry 2022Quote: ... We mixed 10 μL of each fraction with 2 μL of denaturing RNA loading dye and loaded it into a 1.0 cm well PAGE casting plate (Bio-Rad Laboratories, Mississauga, ON, Canada). Urea-PAGE (7.5% ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was diluted at a concentration of 5 ng/µl and 2 µl were loaded in duplicate in a 96-well qPCR plate with the reaction mix comprising 5 µl of Sybr Green master mix (Bio-Rad, Hercules, CA, USA), 2 µl of water and 1 µl of primer mix containing forward and reverse primers for STAT3 and P53 ...
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bioRxiv - Physiology 2024Quote: ... 30 μl of homogenate was transferred in duplicate to a 96-well plate and heat treated at 70°C for 10 min (Bio-Rad T100 Thermal Cycler). Standard curve was prepared using glycerol standard (Sigma ...
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bioRxiv - Systems Biology 2024Quote: ... The cartridges were then washed with 50 µL of 80% MeCN/0.1%TFA and the phosphorylated peptides eluted with 20 µL of 1% NH4OH directly into a 96-well plate (Bio-Rad, Cat. no HSP9601) containing 2.5 µL of 100% formic acid ...
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Gonads or body? Differences in gonadal and somatic photoperiodic growth response in two vole speciesbioRxiv - Neuroscience 2020Quote: ... reactions were carried out in duplo for each sample by using 96-well plates in a Fast Real-Time PCR System (CFX96, Bio-Rad, Hercules, California, United States). Primers for genes of interest were designed using Primer-BLAST (NCBI ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentration was determined using a commercial kit (Pierce™ BCA Protein Assay Kit, Thermo Fischer) and measured at 595 nm with a spectophotometer (BioRad iMarkTM Micro Plate Reader). Each sample was prepared using 30 μg of total proteins combined with the appropriate amount of 5X loading buffer (10% β-Mercaptoethanol ...
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bioRxiv - Microbiology 2020Quote: The pure cultures were grown in TSAYE plates for 24 h and DNA was extracted using 2% Chelex solution (Chelex 100 resin, Bio-Rad laboratory, Hercules, California, USA). The colonies (2-3 mm ...
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bioRxiv - Immunology 2020Quote: ... Volumes of 2 µL of the appropriate cDNA template and 18 µL of the reaction mixture were added to the wells of iQ 96-well semi-skirted PCR Plate (Bio-Rad. Cat. No. 223-9441). Amplification was achieved using Bio-Rad iQ5 Real-time PCR detection System Relative changes in gene expression was determined using delta/delta cycle threshold (2-ΔΔCT ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... aliquots of the samples were organized in a 96-well PCR plate and reverse transcribed to cDNA using the iScript™ Reverse Transcription Supermix kit and protocol (Bio-Rad Laboratories, Hercules, CA). The resulting 96-well plate with cDNA was used as source quantitative real-time polymerase chain reaction (qRT-PCR ...
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bioRxiv - Systems Biology 2022Quote: ... shortly centrifuged (2,000xg, 1 min) and the plate heated at 95°C for 60 min in a thermal cycler (Biorad’s S1000 with 384-well reaction module) at a constant lid temperature of 110°C ...
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bioRxiv - Plant Biology 2024Quote: ... Cycling conditions were optimized by performing 10 µl reactions containing 5.0 µl assay mastermix and 5.0 µl of parental DNA and two non-template water controls (NTCs) in 384-well plate format on a CFX384 qPCR thermocycler (Bio-Rad Laboratories, Hercules, CA, USA). Cycle number was optimized by assessing every two cycles between 26-36x cycles post touchdown of 10 cycles (61–55°C ...
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bioRxiv - Immunology 2023Quote: ... Plasma was thawed on ice and manufacturers instructions were followed to prepare a 96-well plate with samples performed in duplicates and read on a Bio-Plex® 200 System (Bio-Rad Laboratories, Hercules, CA). Results were calculated using Bio-Plex Manager™ Software v6.2 (Bio-Rad ...
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bioRxiv - Genetics 2024Quote: Quantitative PCR (qPCR) was performed in a 96-well plate using a CFX96 Touch Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules, CA, US) with Bio-Rad EvaGreen qPCR Supermix assay ...
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bioRxiv - Plant Biology 2024Quote: ... Tungsten beads were coated with 5 µg of plasmid shot on the plates using the PDS-100/He system (Bio-Rad,Hercules, CA, USA; 1672257) with 1550 psi rupture disks ...
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Induction of autotetraploidy and microbiome associations mediate differential responses to pathogensbioRxiv - Plant Biology 2021Quote: Absolute bacterial abundance was estimated by performing digital droplet PBR (ddPCR) on homogenized whole plant samples randomized within plate columns using the BIO-RAD QX 200 Droplet Reader (Bio-Rad Laboratories, Inc., Hercules, CA, USA) and custom primers to specifically target and amplify Pseudomonas syringae pv ...
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bioRxiv - Molecular Biology 2021Quote: ... All qPCRs assays were run in duplicate in 96 wells plate on a CFX96 Touch™ RealTime PCR Detection System (Bio-Rad Laboratories, Inc., Hercules, CA). qPCR cycle started with an incubation at 95 °C for 10 min followed by 45 cycles of denaturation at 95 °C for 10 sec and an annealing/extension step at 60 °C for 20 sec before a final melt curve from 65-95 °C (0.5 °C increments) ...
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bioRxiv - Microbiology 2023Quote: ... The generated droplet emulsion was transferred to a new 96-well PCR plate and amplified in a C1000 Touch Thermal Cycler (Bio-Rad Laboratories Inc., Pleasanton, CA, USA). The amplification protocol was as follows ...
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bioRxiv - Bioengineering 2021Quote: ... which were gently transferred using a multi-channel pipette into a semi-skirted 96-well plate before heat-sealing (Bio-Rad PX1™ PCR Heat Sealer, 1814000). Target amplification within each droplet was conducted in the C1000 Touch™ Thermal Cycler with 96-Deep Well Reaction Module (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... was collected from each sample and transferred to 96-well ELISA plates for the measurement of the absorbance at 595 nm by using the iMarkTM Microplate Absorbance Reader (Bio-Rad, Alfred Nobel Drive. Hercules, CA, USA).