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Citations for Bio-Rad :
1001 - 1050 of 1114 citations for Recombinant Mouse Tnfrsf10b Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: Total RNA was extracted from mouse tissues using the Aurum Total RNA Fatty and Fibrous Tissue Extraction kit (Biorad: #732-6870). RNA from cells was extracted using Zymo Research R1055 Quick-RNA MiniPrep Kit ...
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bioRxiv - Immunology 2024Quote: ... Membranes were washed then incubated for 1 hour at room temperature with secondary antibody (Goat Anti-Mouse Starbright Blue 520 and Goat Anti-Rabbit Starbright Blue 700, each at 1:2500, Bio-Rad). Blots were analyzed (ChemiDocTM MP Imaging System ...
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bioRxiv - Immunology 2024Quote: ... tissue sections were blocked in 10% normal goat serum for 1 h at room temperature and incubated in rat anti-mouse CD4 (1:200; MCA2691; Bio-Rad) or CD8a (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... Bovine CD4+ T cells were isolated from PBMCs by magnetic-activated cell sorting (MACS) using a mouse anti-bovine CD4 antibody (clone CC8, Bio-Rad), anti-mouse IgG MicroBeads (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2023Quote: ... the membrane was incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (goat anti mouse #1721011; Bio-Rad or goat anti rabbit #1706515; BioRad) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatants were collected from human or mouse brain homogenates after centrifugation and the protein concentration was measured by Bradford Assay (Bio-Rad). Equal amounts of proteins were run on a 4-12% gradient SDS-PAGE gel (Invitrogen ...
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bioRxiv - Developmental Biology 2023Quote: ... The secondary incubation was performed with goat anti-rabbit IgG-HRP conjugate (1:3000) or goat anti-mouse IgG-HRP conjugate (1:3000) (Bio-Rad) for 1 hr at room temperature ...
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bioRxiv - Plant Biology 2023Quote: ... anti-rabbit IgG (H + L) secondary antibody and goat peroxidase-conjugated anti-mouse IgG (H + L) secondary antibody were purchased from Bio-Rad.
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bioRxiv - Cell Biology 2023Quote: ... were used; and as secondary antibody HRP-conjugated anti-rabbit or anti-mouse (both 1:10 000; anti-rabbit (1706515; Bio-Rad); anti-mouse (170-6516 ...
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bioRxiv - Genetics 2023Quote: ... primary antibodies – anti-TY1 and rabbit anti-HA (GTX115044) – and fluorescent secondary antibodies – anti-mouse Starbright Blue 520 (Bio-Rad 12005867) and anti-rabbit Starbright Blue 700 (Bio-Rad 12004162 ...
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bioRxiv - Cell Biology 2023Quote: ... Peroxidase-conjugated goat anti-mouse (170-6516) and peroxidase-conjugated goat anti-rabbit (1706515) secondary antibodies were purchased from Bio-Rad.
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bioRxiv - Bioengineering 2023Quote: ... followed by goat anti-mouse IgG (H+L)-horseradish peroxidase (HRP)-linked secondary antibody (1:1000, 1721011, Bio-Rad, RRID: AB_11125936). Detection of the horseradish peroxidase signal was carried out using Clarity Western enhanced chemiluminescence (ECL ...
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bioRxiv - Immunology 2023Quote: ... and 100 μL of the secondary antibody solution - Goat Anti-Mouse IgG (H+L)-HRP Conjugate (Bio-Rad, Hercules, CA, USA) diluted in a 1:2,000 ratio in the antibody diluent - was then added and the plates were incubated for 1 hour in the dark at RT ...
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bioRxiv - Immunology 2023Quote: ... plates were washed thrice before addition of horseradish peroxidase (HRP)-conjugated anti-mouse IgG (170-6516; Bio-rad, Hercules, California, USA), anti-mouse IgG1 (ab97240 ...
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bioRxiv - Physiology 2023Quote: ... The secondary antibodies were goat anti-mouse IgG-HRP and goat anti-rabbit IgG-HRP conjugate (Bio-Rad, Hercules, CA, USA) for western blotting ...
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bioRxiv - Microbiology 2023Quote: ... mouse cDNA was analyzed in a 10-μl reaction mix consisting of 1x iQ SYBR® Green Supermix (Bio-Rad, USA), 0.2 μM of each primer and 2 μl of template cDNA on a CFX Connect Real-Time System (Bio-Rad) ...
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bioRxiv - Bioengineering 2023Quote: ... probed with a goat anti-mouse or goat anti-rabbit IgG H+ L-HRP conjugated secondary antibody (1:5000-1:10000) (Bio-Rad) for 1 hour ...
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bioRxiv - Neuroscience 2024Quote: ... The following day after washes with TBS-T and incubation with secondary antibodies (Goat Anti-Rabbit or Goat Anti-Mouse IgG (BioRad 1706516) 1:5000 dilution in 10% milk ...
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bioRxiv - Immunology 2021Quote: ... The monolayer was fixed with 4% paraformaldehyde and permeabilized with Triton-X100 and stained with mouse anti-NP IgG1 (clone AA5H, Bio-Rad Antibodies) followed by goat anti-mouse HRP (DAKO ...
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bioRxiv - Molecular Biology 2019Quote: ... The blot was washed 4 times with TBS-T and subsequently incubated with a goat anti-mouse-HRP antibody (Bio-Rad #1706516) for 30 minutes ...
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bioRxiv - Developmental Biology 2019Quote: ... Subsequent analysis was conducted on a 12% SDS-PAGE gel and blots were probed with either mouse α-V5 (SV5-Pk1, 1:1000, Bio-Rad), mouse α-HA (HA.11 ...
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bioRxiv - Neuroscience 2021Quote: ... The blotted membranes were rinsed three times with 0.05% Tween 20 in Tris-HCl-buffered saline (TBST) and incubated with horseradish peroxidase-conjugated goat polyclonal antibody against mouse IgG (Bio-Rad Laboratories) or guinea pig IgG (Bioss Inc. ...
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bioRxiv - Cancer Biology 2021Quote: ... membranes were washed 3x with TBS-T and incubated with either Goat Anti-Mouse IgG (H+L)-HRP Conjugate (Bio-Rad, #1721011) or Goat Anti-Rabbit IgG (H+L)-HRP Conjugate (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... The membrane was washed three times for 10 minutes with 1x PBS and incubated with Goat Anti-Mouse IgG (H+L)-HRP Conjugate (BioRad, #172-1011). Chemiluminescence was induced by incubation with SuperSignal West Femto Maximum Sensitivity Substrate (ThermoFisher ...
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bioRxiv - Microbiology 2021Quote: Bio-Plex assays for cytokines and chemokines were carried out in Bio-Plex 200 system using Bio-Plex Pro Mouse Cytokine Grp I Panel 23-Plex assay kit (Bio-Rad, M60009RDPD) following manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... Secondary antibodies used for Western blotting were: Donkey anti-mouse- and anti-rabbit-HRP (#172-1011 and #170-6515, Bio-Rad, USA). Secondary antibodies used for immunofluorescence were ...
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bioRxiv - Microbiology 2022Quote: ... rabbit polyclonal anti-nsP1 (both validated in our laboratory) [11] and mouse monoclonal anti-GAPDH (Cat. # VMA00046, Bio-Rad, Hercules, CA, USA). Then ...
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bioRxiv - Bioengineering 2022Quote: ... The PVDF membrane was then washed 3 × 10 min with TBST and incubated with horseradish peroxidase (HRP)-conjugated goat anti-mouse (diluted 1:20,000; Bio-Rad, Hercules, CA) secondary antibody for one hour at room temperature followed by 6 × 10 min washes with TBST and detection using the Radiance Plus chemiluminescence substrate (Azure Biosystems ...
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bioRxiv - Cancer Biology 2022Quote: ... slides were washed twice with PBS and incubated with secondary antibodies (Desmin and MYOD1: anti-rabbit-HRP, BioRad 170-6515; Myogenin: anti-mouse HRP, BioRad 170-6516) 1:500 in PBS-1% BSA ...
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bioRxiv - Microbiology 2022Quote: ... cells were heat-fixated in 96-well plates and a sandwich assay with NP-specific monoclonal antibody H16-L10-4R5 (www.atcc.org) and a peroxidase-coupled goat anti-mouse IgG HRP polyclonal antibody (Bio-Rad Laboratories GmbH, Germany) was carried out ...
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bioRxiv - Microbiology 2021Quote: ... The purity of the eluted fractions was assessed by SDS-PAGE and Western blot using a mouse anti-histidine antibody (BioRad, AbD Serotec).
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bioRxiv - Cancer Biology 2022Quote: ... Blots were incubated in secondary antibody for one hour at room temperature (anti-rabbit; Amersham, #NA934) (anti-mouse; Amersham, #NA931) and visualized with Clarity Western ECL Substrate (Bio-Rad, #1705061) on a ChemiDoc Imaging System (Bio-Rad).
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bioRxiv - Developmental Biology 2021Quote: ... Five hundred ng of total RNA (from 21EM15 cells or mouse lenses) or 10 ng of total RNA (from explants) were reverse transcribed using the iScript cDNa Synthesis Kit (Bio-Rad, CA) per the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... equal protein amounts of the lysates (25 μg) were applied to the Bio-Plex Mouse Cytokine 23-plex Assay Kit (Bio-Rad Laboratories) and measured with the BioPlex200 system and BioPlex-Manager 6.2 according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary antibodies used for Western blotting were: Goat anti-mouse- and anti-rabbit-HRP (#172-1011 and #170-6515, Bio-Rad, USA).
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bioRxiv - Cancer Biology 2022Quote: ... membranes were incubated with complementary HRP conjugated secondary antibody (sheep anti-mouse IgG-HRP, 1:10000, GE Healthcare, NA931; goat anti-rabbit IgG-HRP, 1:5000, Bio-Rad, 1706515 ...
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bioRxiv - Immunology 2022Quote: ... To each well 50 µL of 2 µg/mL of biotin-conjugated mouse anti-bovine IFN-γ mAb (CC302, Bio-Rad Antibodies) diluted in PBS was added and plates incubated for 2 hours at RT ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were rinsed 3x for 15 minutes in 1X TBST and incubated in the proper secondary antibody (Goat anti-mouse BioRad #170-6516 or Goat anti-rabbit BioRad #170-6515) for 1 hour ...
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bioRxiv - Physiology 2022Quote: Maternal plasma and SI IEL proteins were assayed for cytokine levels according to manufacturer’s instructions using the Bio-Plex Pro Mouse Cytokine 23-Plex Assay (Bio-Rad, Mississauga, Canada) and the Luminex system (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were washed prior to incubation with a goat anti-mouse StarBright™ Blue 700 secondary (Catalogue #: 12004158; Bio-Rad, Hercules, CA). Gag expression levels were normalized relative to GAPDH levels ...
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bioRxiv - Microbiology 2024Quote: ... the samples were prepared as instructed by the manufacturer and read using Bio-Plex Pro Mouse Cytokine 23-Plex (Bio-Rad, Hercules). Samples were run in technical duplicates ...
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bioRxiv - Cell Biology 2024Quote: ... The proteins were detected using chemiluminescence using horseradish peroxidase–conjugated secondary antibodies of anti-mouse (Bio-Rad # 170-6516, dilution 1:10,000) origin and enhanced chemiluminescence substrate (Thermo Scientific # 32106) ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies used for Western blotting were goat anti-mouse- and anti-rabbit-HRP (#172-1011 and #170-6515, Bio-Rad, USA).
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bioRxiv - Genetics 2023Quote: ... 5µL of urine per mouse was loaded on a 4–15% Mini-PROTEAN TGX Stain- Free Gel (Bio-Rad, Hercules, CA, USA) before Coomassie staining with QC Colloidal Coomassie (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: ... Cytokines were assayed through using Bio-Plex Pro-Mouse Group I Cytokine 23-plex assay kit (M60009RDPD, Bio-Rad, Hercules, California, USA) and the Bio-Plex® 200 System ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were collected following centrifugation of the homogenate at 18,000 gravity for 20 minutes at 4°C and then stored at -80°C until analysis by Bio-Plex Pro Mouse Cytokine 23-plex Assay (Bio-Rad Laboratories) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary antibodies used for Western blotting were: Goat anti-mouse- and anti-rabbit-HRP (#172-1011 and #170-6515, Bio-Rad, USA).
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bioRxiv - Cell Biology 2022Quote: ... The proteins were detected using chemiluminescence using HRP conjugated secondary antibodies of either anti-mouse (Bio-Rad # 170-6516, dilution 1:10,000) or anti-rabbit (GE Healthcare # NA9340 ...
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bioRxiv - Microbiology 2023Quote: ... Heart homogenates were analyzed for cytokine and chemokine levels using a Bio-Plex Pro Mouse Cytokine 23-plex Assay kit (Bio-Rad, #m60009rdpd) and ProcartaPlex mouse IFN-α/IFN-β 2-plex (ThermoFisher ...
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bioRxiv - Microbiology 2023Quote: ... slides were incubated for 30 min at room temperature with primary mouse monoclonal anti-feline coronavirus antibody at 1:400 (BioRad, MCA 2194). The EnVision anti-mouse system was used for visualization according to the manufacturer’s instructions (Agilent).