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Citations for Bio-Rad :
1001 - 1050 of 5608 citations for Mouse Leucine Rich Pentatricopeptide Repeat Containing LRPPRC ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Protein samples were loaded for electrophoresis in 5% or 15% polyacrylamide gels containing sodium dodecyl sulfate (SDS‒PAGE) and transferred onto nitrocellulose membranes using the Trans-Blot Turbo system (Bio-Rad). The membranes were incubated with the primary antibodies listed in Supplemental Table S1 at 4 °C overnight after blocking with Blocking One reagent (Nacalai Tesque) ...
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bioRxiv - Neuroscience 2023Quote: ... Protein transfer to the nitrocellulose membrane was carried out by rapid transfer: the transfer sandwich containing the gel and membrane was placed into a Trans-Blot Turbo Transfer System (Biorad, USA), which was then run at 25 V for 9 minutes ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 50 µg of the membrane protein eluate fraction quantified by BSA were diluted to 32 μl in lysis buffer containing loading Laemmli buffer (Bio-Rad) supplemented with 10% 2-mercaptoethanol ...
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bioRxiv - Immunology 2024Quote: ... cDNA was amplified in a 10 μL reaction mixture containing 5 μL of iTaq SYBR Green Supermix (Bio-Rad, Hercules, CA) and 5 μM each of gene-specific forward and reverse primers ...
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bioRxiv - Synthetic Biology 2023Quote: ... Membranes were then washed 3 times with 1X TBST for 10 minutes each before being transferred to a solution containing enhanced chemiluminescence (ECL) substrate to allow visualization of His-tagged sfGFP (Bio-Rad). The blotted proteins were visualized in a ChemiDoc Imaging System (Bio-Rad ...
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bioRxiv - Pathology 2023Quote: ... and DNA fragment (amplification products containing homologous DNA fragments) in a 4-mm Gene Pulser and electrotransformed by Gene Pulser Xcell system (Bio-Rad). Transgenic parasites were selected with pyrimidine (1μM ...
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bioRxiv - Pathology 2023Quote: ... Each individual and multiplexed TaqMan reaction was performed in a final volume of 10 µL containing 5 µL of Sso Advanced Universal Probes Supermix (Bio-Rad), 600 nM of each primer ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 µl of diluted cDNAs were added to each PCR reaction containing the SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, 1725271) and primers at a final concentration of 300 nM each ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were denatured by boiling for 10 minutes in the DTT containing sample buffer and separated by 4-20% Mini-PROTEIN TGX gels (BIO-RAD) and then transferred to the 0.2 μm PVDF membrane (BIO-RAD) ...
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bioRxiv - Microbiology 2023Quote: ... filtered HEK293F supernatants containing the expressed proteins were flowed (0.5 -1.0 mL/min) over Econo-Column chromatography columns (Bio-Rad, CA, USA) containing 1-2 mL of agarose bound GNL (Vector Laboratories) ...
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bioRxiv - Genomics 2023Quote: ... RT-PCR was performed in triplicates and carried out in 20 µl of reaction mixture containing 10 µl of Fast SYBR® Green Master Mix (Biorad), 0.2 µl (10 pmol/µl ...
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bioRxiv - Biochemistry 2023Quote: ... and the fractions containing the recombinant vaccine candidates were automatically collected using BioFrac™ Fraction Collector (Bio-Rad Laboratories, Hercules, USA) when the 280 nm absorbance was greater than 0.05 ...
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bioRxiv - Genetics 2023Quote: ... The tube containing the mixture was later placed in a CFX96 Real-Time PCR system with a C1000 Thermal Cycler module (Bio-rad) for fluorescence measurements for every 30 seconds per cycle for 60 cycles.
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bioRxiv - Cell Biology 2023Quote: ... The proteins were denatured by boiling for 10 min in the DTT-containing sample buffer and separated by 4–20% Mini-PROTEIN TGX gels (Bio-Rad) and then transferred to the 0.2 µm polyvinylidene difluoride (PVDF ...
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bioRxiv - Microbiology 2023Quote: Dilution series containing equal amounts of protein were prepared and separated on 12% Mini-PROTEAN TGX gels (BioRad, Hercules, CA, USA) by SDS-PAGE and then transferred onto 0.2 µM polyvinylidene difluoride (PVDF ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of protein was denatured at 95°C for 5 min in 1x Laemmli buffer containing 5% β-mercaptoethanol and separated in 4-15% mini-PROTEAN TGX Precast Protein Gels (Biorad) by electrophoresis and blotted onto Nitrocellulose membrane (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... PVDF membranes were washed three times with tween 20-containing tris buffered saline before incubation with HRP-conjugated secondary antibodies (Bio-Rad). Images were detected using a ChemiDoc MP Imaging System (Bio-Rad).
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bioRxiv - Cancer Biology 2023Quote: ... 30 μg of each sample was boiled with 4x Laemmli Sample Buffer containing 10% 2-mercaptoethanol (#1610747, BioRad, Hercules, CA, USA) and run on a Mini-PROTEAN® TGX™ Precast Gel (#4561083 ...
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bioRxiv - Cell Biology 2023Quote: ... 3 μl ten-fold diluted cDNA preparation was mixed in a final volume of 10 μl containing 5 μl iQ SYBR Green Supermix (Bio-Rad) and 4 pmol of each primer (Fw ...
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bioRxiv - Cell Biology 2023Quote: ... PCR products were run at 135V on a 1% agarose gel containing ethidium bromide and bands were observed using a ChemiDoc XRS+ (Bio-Rad). Sequencing of PCR products was performed by Psomagen and resulting sequencing files were analyzed with Geneious software using NCBI gene data.
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bioRxiv - Biochemistry 2024Quote: ... Fractions containing protein were pooled and desalted using the Econo-Pac® 10-DG Desalting Columns (Bio-Rad, Cat#732-2010). To this end ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was then eluted in 3 mL buffer A containing 300 mM imidazole before applying the eluate onto Econo-Pac 10DG column (Bio-Rad) equilibrated with HBS to remove imidazole ...
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bioRxiv - Microbiology 2024Quote: ... 2 µL of each diluted RT and NRT sample was added to a 22 µL reaction mixture containing 11 µL of QX200TM ddPCR TM Evagreen Supermix (Bio-Rad) and 1.1 µL of each 2 µM ddPCR primers (Table S6) ...
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bioRxiv - Immunology 2024Quote: ... was performed with 100 ng of cDNA in a 20-μL reaction mixture containing SsoAdvanced universal SYBR green supermix (#1725274; Bio-Rad) using phagocytosis PrimePCR plates (#10047255 ...
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bioRxiv - Immunology 2023Quote: ... The second kit used was the Bio-Plex Pro Serology Reagent Kit (Bio-Rad, Cat No. 12014777), with human IgA positive and negative controls (Bio-Rad ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were detected using secondary HRP-conjugated α-mouse antibody and enhanced chemiluminesence reagent (Bio-Rad).
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bioRxiv - Molecular Biology 2021Quote: ... and incubated with goat anti-mouse or anti-rabbit IgG conjugated with horseradish peroxide (Bio-Rad). After washing of the filter ...
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bioRxiv - Molecular Biology 2021Quote: ... The blots were then placed in secondary antibodies (goat anti mouse or goat anti-rabbit, BioRad), and detected using enhanced chemiluminescence (Perkin Elmer Life Sciences ...
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bioRxiv - Molecular Biology 2020Quote: ... We used horseradish peroxidase coupled secondary antibodies (α-mouse and α-rabbit, Bio-Rad, 1:2000). Blots were developed using an enhanced chemiluminescence kit (Amersham ...
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bioRxiv - Immunology 2021Quote: ... followed by detection with mouse anti-human secondary antibody conjugated to HRP (1721050, Bio-Rad Laboratories). HRP binding was determined based on reaction with TMB substrate (34028 ...
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bioRxiv - Biochemistry 2019Quote: ... Goat anti-rabbit (170-6515) and anti-mouse (626520) Ig secondary Abs were purchased from BioRad and Life Technologies respectively ...
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bioRxiv - Immunology 2019Quote: ... and a primary rat anti-mouse CD68 antibody (activated microglia/macrophages, 1:200 dilution, MCA1957, Biorad) with secondary goat anti-rat Alexa-Fluor 555 (1:200 dilution ...
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bioRxiv - Cell Biology 2019Quote: ... Secondary antibodies used for the immunoblotting are Goat anti-Mouse IgG-HRP (Bio-Rad, 170-6516) and goat anti-Rabbit IgG-HRP (Dako ...
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bioRxiv - Microbiology 2020Quote: ... Supernatant fractions were then assayed using the BioPlex Pro Mouse Cytokine 23-Plex (Bio-Rad Laboratories) for the presence of IL-1α ...
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bioRxiv - Bioengineering 2020Quote: ... cells were washed in PBS and were treated with anti-mouse SR-A1 (Bio-Rad Laboratories). For flow cytometric analysis of single cell suspensions prepared from harvested organs (mouse studies) ...
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bioRxiv - Cell Biology 2021Quote: ... Secondary goat anti-mouse or anti-rabbit horseradish peroxidase-conjugated antibodies (Bio-Rad, Hercules, United States) were used at a dilution of 1:10’000 ...
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bioRxiv - Cell Biology 2021Quote: ... Horseradish peroxidase-conjugated goat anti-rabbit and anti-mouse secondary antibodies were obtained from Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2020Quote: ... and goat α-mouse or α-rabbit IgG-AP conjugate antibodies (Bio-Rad Laboratories, #1706520, #1706518). MG132 (Enzo Life Sciences ...
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bioRxiv - Neuroscience 2020Quote: ... and the Bio-Plex Pro Mouse Cytokine 23-Plex Assay (Cat# M60009RDPD; Bio-Rad, Carlsbad, California), respectively ...
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bioRxiv - Molecular Biology 2021Quote: ... Secondary antibodies fused to HRP were used for detection (Goat anti-mouse HRP 1:3000, BioRad; Goat anti-rat HRP 1:3000 ...
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bioRxiv - Microbiology 2020Quote: ... and 1: 25000 diluted secondary HRP conjugated Goat anti-mouse IgG (H+L) (BioRad, Mississauga, Canada). The blot was developed using Clarity Western ECL substrate (BioRad ...
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bioRxiv - Microbiology 2020Quote: ... The plates were washed and incubated with mouse anti-His (Bio-Rad MCA-1396; 1:1000) followed by horseradish peroxidase (HRP)-conjugated goat anti-mouse secondary antibody (MerckMillipore AP124P ...
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bioRxiv - Microbiology 2021Quote: ... followed by secondary antibodies (1:4000 donkey anti-goat, Abcam; 1:4000 goat anti-mouse, Biorad). Immunoblots were developed using SuperSignal West Pico PLUS Chemiluminescent Substrate (ThermoFisher).
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bioRxiv - Biochemistry 2020Quote: ... followed by a incubation with a second Goat Anti-Mouse IgG-HRP conjugate antibody (Bio-Rad) diluted to 1 ...
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bioRxiv - Cell Biology 2019Quote: Sections of mice peritoneum were stained with primary rat anti-mouse CD68 antibody (MCA1957B, Bio-Rad), rabbit anti-mouse CD38 antibody (bs-0979R ...
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bioRxiv - Developmental Biology 2020Quote: ... The following primary antibodies and concentrations were used: mouse anti-CD44 (1:500, Bio-Rad MCA5762), mouse anti-Laminin (1:500 ...
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bioRxiv - Cancer Biology 2021Quote: ... Secondary anti-rabbit (#170-6515) and anti-mouse (#170-6516) antibodies were purchased from BIO-RAD.
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bioRxiv - Cancer Biology 2020Quote: ... Secondary anti-mouse and anti-rabbit antibodies were coupled to horseradish peroxidase (Biorad, Hercules, CA, USA).
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bioRxiv - Genetics 2021Quote: ... tissue sections were stained with F4/80 rat anti mouse antibody clone A3-1 (Bio-Rad Laboratories ...
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bioRxiv - Immunology 2020Quote: ... Plasma cytokine levels were analyzed by 23-plex mouse cytokine assay according to manufacturer’s instructions (Biorad). Plasma IFNα (all sub-types ...