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Citations for Bio-Rad :
1001 - 1040 of 1040 citations for EGF Mouse His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: Serum samples collected from mice were tested in duplicate in a multiplex immunoassay using magnetic beads (Mouse Th1-Th2 8-plex Bio-Rad, Hercules, CA) using the Luminex MAGPIX® system) ...
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bioRxiv - Cell Biology 2023Quote: ... mouse monoclonal anti-MF20 (DSHB, Cat# MF 20, dilution 1:20) rabbit polyclonal anti-Calcitonin Receptor (Bio-RAD, Cat# AHP635, dilution 1:2000), chick anti-GFP (abcam ...
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bioRxiv - Immunology 2023Quote: Extracted whole-kidney protein was diluted in PBS to 900 µg/mL and subjected to a Bio-Plex Mouse Cytokine 23-plex Assay (#M60009RDPD, Bio-Rad, Hercules, CA), according to the manufacturer instructions ...
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bioRxiv - Physiology 2023Quote: ... Twenty µg (islet sample) or 2 µg (mouse liver sample: positive control) was mixed with 10uL of Laemmli Sample Buffer (Bio-Rad, Hercules, CA) and denatured at 95°C for 10 min ...
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bioRxiv - Physiology 2023Quote: ... Cytokine concentrations in BAL fluid were measured using the multiplex Bio-Plex 200 System and Bio-Plex Pro Mouse Cytokine/Chemokine Immunoassays (Bio-Rad, Hercules, CA).
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bioRxiv - Molecular Biology 2023Quote: ... primary antibodies and proteins were detected by incubation at a 1:5000 dilution with secondary horseradish peroxidase (HRP)- conjugated antibodies (#170-6516, goat anti-mouse and 170-6515; goat anti-rabbit from Bio-Rad, Hercules, USA). The reactive protein bands were visualized using the WesternBright ECL HRP substrate (Advansta ...
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bioRxiv - Molecular Biology 2023Quote: ... and optic density of the bands corresponding to mouse PRM1 and PRM2 was quantified using Quantity One 1-D analysis software (BioRad, Hercules, CA, USA) to calculate the PRM1/PRM2 ratio ...
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bioRxiv - Immunology 2023Quote: ... Primary antibody staining was performed overnight at 4&C using rabbit anti-mouse Iba-1 (1:500; Wako Chemical Cat #019-19741) and CD68 (1:200; Bio-Rad Cat #MCA1957) with secondary staining performed for 45 minutes RT using Alexa Flour 594 donkey anti-rabbit (1:400 ...
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bioRxiv - Molecular Biology 2023Quote: ... and incubated for 1 hour with fluorescent-tagged secondary antibody diluted in PBST (Goat anti-Rabbit IgG StarBright™ Blue 700 or Goat anti-Mouse IgG DyLight™ 800, Bio-Rad). Finally ...
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bioRxiv - Cell Biology 2024Quote: Proteins were detected by chemiluminescence following Western blotting using HRP conjugated secondary antibodies of anti-mouse (Bio-Rad # 170–6516; dilution 1:10,000), anti-rabbit (GE Healthcare # NA9340 ...
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bioRxiv - Microbiology 2024Quote: ... membranes were incubated in an appropriate secondary antibody: anti-Rabbit IgG-HRP Conjugate #1706515 and anti-Mouse IgG (H + L)-HRP Conjugate #1706516 (Bio-Rad, Hercules, CA). Image Quant TL Toolbox v8.1 software (Amersham Bioscience ...
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bioRxiv - Neuroscience 2024Quote: ... The following primary antibodies were used (see also Supplementary Table 1 for details): mouse monoclonal IgG1 anti-βCYA (Bio-Rad, clone 4C2, RRID:AB_2571580), mouse monoclonal IgG2b anti-γCYA (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... Non-diluted supernatant of tissue homogenate (50 μl) was applied to Bio-plex pro mouse cytokine & chemokine assay kit (Bio-Rad, #M60-009RDPD).
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bioRxiv - Neuroscience 2019Quote: ... The membrane was incubated using appropriate HRP secondary antibodies (donkey anti-rabbit 711-035-152, donkey anti-mouse 715-035-150, Jackson Immunoresearch Laboratory and Biorad ChemiDoc XRS System) or fluorescent secondary antibodies (IRDye 800 CW ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Then ran the assay in accordance with the specification (Bio-Plex Pro Mouse Cytokine Grp, Catlog NO. #M60009RDPD, LOT. 64212941, Bio-Rad Laboratories, Inc., USA). Read the plate at Bio-Plex MAGPIX System (Bio-Rad Laboratories ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... while insulin levels were assayed using the quantitative Bio-Plex Pro™ Mouse Diabetes 8-Plex immunoassay (#171F7001M; Bio-Rad Laboratories, Hercules, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The plate was washed 3 times prior to adding 50 μl of 1:1000 diluted Goat anti-Mouse IgG H+L-HRP antibody (Bio-Rad, Cat# 172-1011) to the plate and incubated for 1 h at RT ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were then washed three times for 5 min with 1x TBST and incubated with either an anti-mouse IgG- HRP-conjugate (1:5000, Bio-Rad Cat#172-1011) or an anti-rabbit IgG-HRP-conjugate (1:5000 ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were then incubated for 1 h with either anti-mouse or anti-rabbit secondary antibodies conjugated to horseradish peroxidase (Bio-Rad Laboratories, Hercules, CA). Bands were visualized and subjected to densitometric analysis using an Odyssey Fc system (LI-COR Biotechnology ...
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bioRxiv - Cell Biology 2021Quote: ... or mRNA isolated from cell lines or primary mouse cells were proceeded to RT-qPCR using iTaq universal SYBR green one step kit (Bio-Rad, Cat. No. 1725150). DNA obtained from ChIP was proceed to RT-qPCR using iQ SYBR green supermix (Bio-Rad ...
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bioRxiv - Immunology 2020Quote: Staining of 2×105 cells per well was performed with mouse anti-human CD79α (clone HM57, Bio-Rad AbD Serotec, Puchheim, Germany, 1:100) for identification of B cells and Alexa Fluor 647-conjugated rat anti-human CD3∊ (clone CD3-12 ...
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bioRxiv - Immunology 2023Quote: Vaginal mucosa cells and splenocytes were analyzed by flow cytometry using the following antibodies: mouse anti-guinea pig CD8 (clone MCA752F, Bio-Rad Laboratories, Hercules, CA), mouse anti-guinea pig CD4 (clone MCA749PE ...
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bioRxiv - Microbiology 2024Quote: ... FLAG-tagged proteins were detected using anti-DYKDDDDK tag primary antibodies (Wako, 1E6, 1:5,000 dilution) combined with anti-mouse-HRP secondary antibodies (Bio-Rad, #1706516, 1:5,000 dilution). SigA proteins were detected using anti-SigA85 (1:10,000 dilution ...
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bioRxiv - Microbiology 2023Quote: ... Revelation was performed using a second antibody coupled to the HRP (anti-rabbit or anti-mouse IgG conjugated to horseradish peroxidase, Bio-Rad, 1:5,000 dilution) and detected using the Clarity Western enhanced-chemiluminescence (ECL ...
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bioRxiv - Biochemistry 2023Quote: ... Then the membrane was incubated with mouse monoclonal antibodies against PAR for 60 min in PBS-T/5% milk followed by incubation with the secondary anti-mouse antibodies conjugated with horseradish peroxidase (Bio-Rad, Hercules, CA, USA) for 60 min in the PBS-T/5% milk solution ...
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bioRxiv - Plant Biology 2023Quote: ... followed by addition of primary antibodies at 4 °C overnight with rotation (mouse anti-ϕ3-actin [Proteintech, 66009-1-Ig], 1:1000, and rat anti-χξ-tubulin [YOL1/34, MCA78G, Bio-Rad], 1:1000). The specimen was washed three times with PBST and incubated with secondary antibodies (anti-mouse ...
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bioRxiv - Cell Biology 2024Quote: ... Anti-rabbit or anti-mouse (Li-Cor BioSciences; 1:10000) or anti-Rabbit IgG (H+L)-HRP Conjugate (Bio-Rad; 170-6515; 1:5000) secondary antibodies were used for visualization and quantification ...
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bioRxiv - Cell Biology 2022Quote: ... in PBS and incubated overnight at 4°C in primary antibodies: anti-human actin-β primary monoclonal antibody produced in mouse (1:100; Bio-Rad Laboratories Inc. MCA57766A) and non-muscle myosin II produced in rabbit (1:100 ...
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bioRxiv - Immunology 2022Quote: ... Cytokine production was measured in lung homogenates via Bioplex using the Luminex™ Magpix™ multiplexing platform with the Bio- Plex Pro Mouse Cytokine 23-plex assay (Bio-Rad, Hercules, CA) as directed ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were incubated with secondary antibodies for 1 hour at room temperature (anti-rabbit or anti-mouse, 1:10.000, Bio-Rad, anti-goat, 1:7500, Pierce). Antibody binding was detected by chemiluminescence(Clarity kit ...
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bioRxiv - Developmental Biology 2022Quote: Genomic DNA extracted from mouse tail tips (H. Liu et al., 2019) was subjected to PCR performed in a thermal cycler (T100, Bio-Rad Laboratories, Hercules, CA, USA). The WT and Ssh2 KO alleles were assayed by primers ...
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bioRxiv - Developmental Biology 2024Quote: ... Horseradish peroxidase (HRP)-conjugated secondary antibodies used were goat anti-rabbit IgG or anti-mouse IgG (Bio-Rad, 170-6515 or 170-6516, 1:3,000). To prevent signal overlap from IgG heavy chain with eRF1 ...
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bioRxiv - Immunology 2023Quote: Plasma from control and infected mice was used at a 1:2 and 1:4 dilution with the Bio-Plex Pro Mouse Cytokine 23 Plex Immunoassay (Bio-Rad Laboratories, Inc., CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant was collected and the level of the cytokines and chemokines was estimated by using Bio-Plex ProTM Mouse Cytokine standard 23-Plex (Bio-Rad, USA, Cat. no. 10014905). The kit was inbuilt and supplemented with 23 analytes ...
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bioRxiv - Cell Biology 2024Quote: ... Relative expression of target gene levels were calculated according to the ΔΔCT method normalized to mGapdh levels and then wild-type mouse cohort using Bio-Rad CXF Maestro 2.3 software (Bio-Rad Laboratories Inc., Hercules, CA, U.S.).
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Glucagon-like peptide 1 (GLP-1) and Adiponectin were measured using quantitative Bio-Plex Pro™ Mouse Diabetes 8-Plex immunoassay (#171F7001M; Bio-Rad Laboratories, Hercules, CA, USA) and Bio-Plex Pro Mouse Diabetes Adiponectin assay #171F7002M (Bio-Rad Laboratories ...
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bioRxiv - Bioengineering 2024Quote: ... the membrane was incubated with HRP-conjugated anti-mouse secondary antibody (1:1000) and streptactin antibody (specific to the standard protein marker – Bio-Rad, Cat# 1610376; 1:1000 dilution) for 1 h at 25°C before washing it six more times ...
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bioRxiv - Bioengineering 2023Quote: ... The membrane was then washed thrice for 5 mins in PBS-Tween 20 before incubation with an HRP-conjugated anti-mouse secondary antibody (1:1000) and streptactin antibody (specific to the standard protein marker – Bio-Rad, Cat# 1610376; 1:1000 dilution) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... Secondary horseradish peroxidase-coupled antibodies (1:3000, Pierce, α-mouse #1858413, α-rabbit #1858415) were applied prior to ECL-based chemiluminescence (Biorad #1705061 and ThermoFisher #34094) detected using a Fusion Fx instrument (Vilber Lourmat) ...
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bioRxiv - Immunology 2020Quote: ... a total of 6 × 107 cells was incubated in staining buffer with mouse anti-pig CD4 alpha (clone MIL17, Bio-Rad AbD Serotec, Puchheim, Germany, 1:50) at 4°C for 20 min ...