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Citations for Bio-Rad :
1001 - 1050 of 5911 citations for 5M Betaine Solution PCR Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... DNA was purified by standard phenol chloroform extraction protocol and assayed by quantitative PCR on a CFX96 real-time PCR system (Bio-Rad) or LC96 (Roche ...
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bioRxiv - Microbiology 2021Quote: ... PCRs were performed on a CFX96 Touch Real-Time PCR Detection System using white Hard Shell 96-well PCR plates and Microseal ‘C’ Adhesive seals (Bio-Rad) or 0.2 mL white 8-tube PCR strips and optical caps (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: ... Samples were assayed by Taqman multiplex real-time PCR using TaqMan Universal Master Mix II and a CFX384 Touch™ Real Time PCR detection system (BioRad). A standard curve was obtained using an in vitro transcript derived from a synthetic plasmid that contained all PCR targets ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative RT-PCR was performed as described previously (Han et al., 2018) with a CFX96 real-time PCR detection system (Bio-Rad) using iTaq SYBR Green Supermix with ROX (Bio-Rad) ...
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bioRxiv - Molecular Biology 2021Quote: ... and subsequent quantitative PCR (qPCR) with SYBR green I using a MyiQ single-color real-time PCR detection system (Bio-Rad). Analysis of the reverse transcription (RT)-qPCR data was performed using the 2-ΔΔCT method (Livak and Schmittgen ...
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bioRxiv - Neuroscience 2020Quote: ... Total RNA was used for cDNA synthesis using iScript kit and real-time qRT-PCR (quantitative real-time PCR) were done in triplicate with AB PowerSYBR green mix on an iCycler (Bio-Rad). The primers used for qRT-PCR are listed in Table 1.
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bioRxiv - Microbiology 2023Quote: ... and then diluted and subjected to quantitative PCR using TransStart Green qPCR SuperMix (TransGen Biotch) in a CFX96 Touch Real-Time PCR Detection System (Bio-Rad). Primers used appear in Table S1.
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bioRxiv - Microbiology 2023Quote: ... Droplet digital PCR reactions were performed using the QX200 Droplet Digital PCR system with ddPCR SuperMix for Probes (no dUTP) (Bio-Rad). The RS ratio was calculated from each duplexed reaction by the QX200 Droplet Digital PCR system software (QuantaSoft AP ...
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bioRxiv - Molecular Biology 2022Quote: Transcript abundance was quantified for each sample relative to a normalization transcript using the PerfeCTa SYBR Green FastMix (Quantabio) in 96-well PCR plates (Hard-Shell 480 PCR Plates, Bio-Rad) measured in a LightCycler 480 (Roche) ...
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bioRxiv - Bioengineering 2023Quote: ... qRT-PCR was performed using SYBR Green Realtime PCR Master Mix (QPK-201, TOYOBO) on a CFX Connect Real-Time PCR Detection System (Bio-Rad). All samples were run in two technical replicates and normalized with respect to GAPDH levels using the 2-ΔΔCt method ...
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bioRxiv - Microbiology 2023Quote: ... PCR was performed in a 96-well standard (0.2mL) plate on a CFX96 Real-Time PCR Detection System (Bio-Rad Laboratories). Expression patterns were revealed using the delta-delta Ct method with SmsR1491 or SmsR847 as the normalization gene.
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bioRxiv - Developmental Biology 2023Quote: Gene expression was measured via quantitative real-time PCR (qRT-PCR) using the iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories) and the ΔCt method ...
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bioRxiv - Cell Biology 2023Quote: ... The total cDNA obtained was diluted 1:10 and 2 µl was used for qRT-PCR by using gene specific primer and SYBR green PCR mix (BIO-RAD) on BIO-RAD CFX96 real time PCR machine ...
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bioRxiv - Neuroscience 2022Quote: ... PCR of each target was performed in triplicate using the CFX384 Touch Real-Time PCR Detection System (Bio-Rad, Hercules, CA) with the following thermocycling conditions ...
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bioRxiv - Neuroscience 2022Quote: ... To verify the identified multiexonic deletion of SLC7A5 we performed a quantitative PCR (qPCR) using CFX ConnectTM Real-Time PCR Detection System (Bio-Rad) with primers spanning the deleted region ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative reverse transcriptase PCR (qRT-PCR) was performed for siRNA-mediated Brca1 and Brca2 depletion on qPCR CFX 96 Thermocycler (Bio-Rad) using gene-specific primers (Table EV 3) ...
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bioRxiv - Molecular Biology 2024Quote: ... Real-time PCR was performed with SYBR Green mix (Genstar, Cat# A301-01) on a CFX96 real-time PCR system (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... followed by qPCR with PowerUp SYBR green PCR master mix on a CFX Connect Real-Time PCR Detection System (Bio-Rad). All reagents ...
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bioRxiv - Plant Biology 2024Quote: ... The PCRs were executed using the C1000 Touch PCR with the CFX96 Real-Time Optics Module qPCR System (BIO-RAD, USA). The qPCR primers were designed using Quant prime online tool ...
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bioRxiv - Genomics 2024Quote: ... KLF14 was evaluated using real-time PCR on CFX96 Real-Time system with SYBR Green Fast qRT-PCR mix from Bio-Rad. The reaction protocol involved priming at 25°C for 5min ...
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bioRxiv - Genomics 2024Quote: ... 10 ng of cDNA was used per PCR reaction with Perfecta SYBR Green Fastmix (Quanta BioSciences, 95072) using the CFX96 Real-Time PCR Detection System (Bio-Rad). All amplicon products were verified by melting curve analysis ...
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bioRxiv - Cell Biology 2024Quote: ... Quantitative real-time PCR reactions were carried out using the SYBR Green PCR master mix and ROX Passive Reference Dye (Bio-Rad) with Micro-Amp Optical 384-well plates ...
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bioRxiv - Molecular Biology 2023Quote: Quantitative PCR of the target mRNAs and miRNAs was carried out on a CFX96 real-time PCR detection system (Bio-Rad) using SYBR Green Premix Pro Taq HS qPCR kit (Accurate Biology ...
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bioRxiv - Microbiology 2023Quote: A volume of 5 μL of cDNA was used for quantitative PCR (qPCR) on a CFX Connect Real- Time PCR Detection System (Bio-Rad). TaqMan and SYBR Green qPCRs were performed as described previously (Benschop et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... The relative levels of gene transcripts compared with the control gene 18S were determined by quantitative real-time PCR using SYBR Green PCR Supermix (Bio-Rad) and specific primers on a 7,500 fast real-time PCR system (Applied Biosystems ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative real-time PCR assay was carried out using gene-specific fluorescently labelled probes and using CFX96 PCR system by Bio-Rad. The nucleotide sequences used for real-time qPCR analysis are showed in Table 1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Droplets were transferred to a 96-well PCR plate and heat-sealed using the PX1 PCR plate sealer (Bio-Rad Laboratories). Droplets were amplified using the following cycling conditions ...
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bioRxiv - Cell Biology 2022Quote: ... Real-time PCR analyses were performed on a MyiQ Single-Color Real-Time PCR detection system (Bio-Rad, Veenendaal, the Netherlands), employing SYBR Green chemistry (SensiMix™ SYBR® & Fluorescein Kit ...
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bioRxiv - Immunology 2022Quote: ... Droplet digital PCR was performed in quadruplicates with QX200 ddPCR EvaGreen Supermix and QXDx AutoDG Droplet Digital PCR System (Bio-Rad). Copy numbers of GAPDH transcripts were used for normalization.
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bioRxiv - Neuroscience 2023Quote: ... Quantitative real-time PCR was performed using Corbett Research RG-6000 Real Time PCR Thermocycler with SYBR Green Supermix (Bio-Rad). The following primer sequences were used ...
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bioRxiv - Immunology 2023Quote: ... China) and analyzed using SYBR Green I based real-time quantitative PCR method on a CFX96 RT-PCR system (Bio-Rad). Expression levels were determined using the 2-Δ Δ Ct method and ActB or Krt5 as internal standards ...
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bioRxiv - Genomics 2023Quote: ... 2.0 μL of cDNA was used per PCR reaction with Perfecta SYBR Green Fastmix (Quanta BioSciences, 95072) using the CFX96 Real-Time PCR Detection System (Bio-Rad). All primers were designed to be highly specific using NCBI’s primer blast tool and amplicon products were verified by melt curve analysis ...
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STAT3 protects HSCs from intrinsic interferon signaling and loss of long-term blood-forming activitybioRxiv - Immunology 2023Quote: ... Stat3 deletion or differential gene expression between experimental groups was confirmed by performing qRT-PCR in a CFX real-time PCR machine (Bio-Rad) using 2X SYBR Select Master Mix (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2023Quote: ... The qRT-PCR experiments were performed in quadruplicates using SYGREEN BLUE qPCR MIX (PCRBIO) with Chromo4 Real-Time PCR Detection System (Bio-Rad). Target gene expression level was quantified by the 2−ΔΔct method with UBIQUITIN10 as internal control ...
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bioRxiv - Physiology 2024Quote: ... Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) was performed using a CFX96 Real-Time PCR Detection System (Bio-Rad) with Power SYBR Green PCR Master Mix (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... The synthesized cDNAs were then subjected to PCR amplification using ChamQ SYBR qPCR Master Mix (Vazyme) in CFX Real-Time PCR system (Bio-Rad). The relative mRNA levels of DDHD2 were quantified using the 2−ΔΔCT method with normalization to GAPDH ...
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bioRxiv - Cell Biology 2024Quote: ... Real-time quantitative PCR (RT-qPCR) was performed using SYBR green in a CFX Duet Real-time PCR machine (Bio-Rad). Gene expression was quantified by (ΔΔCq ...
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bioRxiv - Microbiology 2024Quote: ... followed by qPCR with PowerUp SYBR green PCR master mix on a CFX Connect Real-Time PCR Detection System (Bio-Rad). qPCR was performed using 1 μL of eluted DNA per reaction ...
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bioRxiv - Microbiology 2024Quote: ... Transcript levels of selected genes were quantified by real-time quantitative PCR (qPCR) using a CFX Connect Real-Time PCR Detection System (Bio-Rad), using appropriate oligonucleotides detailed in Table S2 ...
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bioRxiv - Molecular Biology 2024Quote: ... and expression of the indicated genes was analyzed by quantitative RT-PCR using the CFX Opus 96 Real-Time PCR System (Bio-Rad) (see Table S1 for primer sequences).
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bioRxiv - Plant Biology 2024Quote: ... graminicola genomic DNA was quantified by quantitative PCR using Biozym Blue S’Green qPCR Kit (Biozym Scientific, Germany) in a CFX RT-PCR detection system (Bio-Rad, USA) and specific primers for the histone 3 (CgH3 ...
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bioRxiv - Plant Biology 2024Quote: ... higginsianum genomic DNA quantitation was performed by quantitative PCR using Biozym Blue S’Green qPCR Kit (Biozym Scientific, Germany) in a CFX RT-PCR detection system (Bio-Rad, USA) and specific primers for ChTrpC gene (Supplementary Table S1) ...
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bioRxiv - Biochemistry 2024Quote: ... Differential scanning fluorimetry melting curves were measured in 96-well plates using a CFX96 Touch RT-PCR System or a CFX Opus 96 RT-PCR System (Bio-Rad), all samples were heated over a temperature gradient from 15 °C to 95 °C at increments of 0.5 °C every 30 seconds ...
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bioRxiv - Microbiology 2024Quote: ... Quantitative RT-PCRs (qRT-PCRs) were carried out in triplicate with three technical replicates with the SsoAdvanced Universal SYBR Green kit (Bio-Rad) on a CFX384 Touch Real-Time PCR Detection System (BioRad) ...
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bioRxiv - Immunology 2024Quote: ... Amplification by real-time PCR was performed on a CFX Connect Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules, CA) using SYBR Green PCR Master Mix Kit (Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2024Quote: Transcript abundance was quantified for each sample relative to a normalization transcript using the PerfeCTa SYBR Green FastMix (Quantabio) in 96-well PCR plates (Hard-Shell 480 PCR Plates, Bio-Rad) measured in a LightCycler 480 (Roche) ...
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bioRxiv - Zoology 2024Quote: Quantitative real-time PCR was performed on a C1000 Touch Thermal Cycler with a CFX96 Touch Real-Time PCR Detection System (Bio-Rad) using SsoAdvanced™ Universal SYBR® Green Supermix (Bio-Rad) ...
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bioRxiv - Neuroscience 2024Quote: ... Expression levels of qRT-PCR samples was analyzed using the CFX Real-Time PCR Detection System and CFX Manager Software (Bio-Rad). Relative enrichment of marker genes was evaluated using the comparative CT method ...
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bioRxiv - Neuroscience 2024Quote: Real-time quantitative PCR reactions were conducted in a 96-well plate using a CFX96 Touch Real-Time PCR System (Bio-Rad). Each reaction was performed in a 10 μL volume containing 1× Luna Universal One-Step Reaction Mix plus 1× Luna WarmStart® RT Enzyme Mix (Cat ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Gene expression was detected using cDNA as a template in a SYBR Green real-time PCR reaction on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad; primer sequences are provided in Table S1) ...