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Citations for Bio-Rad :
1001 - 1050 of 1484 citations for 5 Alpha dihydroprogesterone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Samples were boiled at 95 °C for 5 minutes and separated on precast 4-20% Criterion TGX gels (Bio-Rad). The competition of IA-rhodamine was analyzed by in-gel fluorescent signal using a ChemiDoc MP (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... Detection of proteins was achieved following 5 min incubation with Enhanced Chemiluminescence (ECL) reagent and exposed using a ChemiDoc imaging system (BioRad). Loading control was performed by normalizing the target protein to the stain-free images of total protein ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were boiled at 95 °C for 5 min before being loaded onto a polyacrylamide gel (Cat# 4561094, Bio-Rad). Proteins were blotted onto a PVDF membrane (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was diluted 1:4 and 4.3 µL of cDNA was mixed with 5 µL iTaq Universal SYBR Green Supermix (BioRad 1725124) and 0.7 µL of 5 µM forward and reverse qPCR primers targeting the gene of interest (333 nM final primer concentration ...
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bioRxiv - Neuroscience 2023Quote: ... The samples adjusted to contain equal amounts of protein (10 μg or 12 μg) were next denatured (5 min at 95°C) in loading buffer (#1610747, BioRad) and run on 10% TGX (#4561034 ...
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bioRxiv - Plant Biology 2024Quote: ... Blots were washed four times with PBS-T for 5 minutes and chemiluminescent signals were detected using Clarity Western ECL Substrate (BioRad,), and visualised using the ImageQuant® LAS-4000 imager (GE Healthcare ...
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bioRxiv - Neuroscience 2024Quote: ... boiled for 5 min at 95 °C and then loaded on 4–15% Mini-PROTEAN TGX Precast Gels (Bio-Rad). Gels were transferred onto polyvinylidene fluoride membranes using the Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: ... and ∼140-150nt DNA fragments were then size-selected by gel electrophoresis with 5% TBE gels (Bio-Rad, Cat #4565015) following NEB library prep kit protocol ...
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bioRxiv - Cell Biology 2024Quote: ... before heating at 95°C for 5 minutes and finally loaded onto 4-20% polyacrylamide gel (Bio-Rad, Cat # 4561094). Samples were then transferred to a PVDF membrane using the Trans-Blot® Turbo™ Transfer System (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... 60 mM EDTA pH 7.0 containing 5% β-mercaptoethanol and 1 mg/ml zymolyase 100T) at 40 °C and aliquoted to plug molds (BioRad) to prepare 20 agarose plugs ...
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bioRxiv - Immunology 2024Quote: ... Stained cells were resuspended in FACS buffer and acquired with a 5-laser Bio-Rad ZE5 flow cytometer (Bio-Rad). FACS data were analyzed using FlowJo version 10.9 software (BD Biosciences ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples (25 µl total volume) were heated 5 minutes at 95 °C and loaded onto an AnyKD precast MiniProtean TGX Stain Free gel (Biorad) or an 15% SDS-PAGE gel ...
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bioRxiv - Pathology 2024Quote: ... Total reaction volume was 10 µl and contained 5 µl of iTaq Universal SYBR green one-step kit (Bio-Rad), 300 nM of forward and reverse primers (Table S5 ...
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bioRxiv - Genetics 2024Quote: cDNA was generated using 5 ng of total RNA using iScript Reverse Transcriptase supermix (cat#1708841) and C1000 Touch Thermal Cycler (Biorad) as per manufacturer guidelines ...
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bioRxiv - Cell Biology 2024Quote: ... 10 µg of protein was boiled for 5 min at 95°C and then loaded on a 4-15% SDS gel (Biorad).
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were washed 3 times for 5 min with 1X TBS 0.1% Tween 20 and unsaturated fluorescent signal was acquired using Chemidoc MP imager (Bio-Rad) with identical settings for samples to be compared within an experiment.
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bioRxiv - Systems Biology 2024Quote: ... beads were resuspended in 1 mL of washing buffer (50 mM HEPES pH 8.0, 150 mM NaCl, 5 mM EDTA) and transferred to BioSpin membrane columns (Bio-Rad). Beads were washed 3x with 1 mL washing buffer ...
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bioRxiv - Biochemistry 2024Quote: ... at a 20-90°C gradient (increment: 0.3 °C, hold for 5 s before read) on a Real-time PCR system (Bio-Rad).
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bioRxiv - Cell Biology 2024Quote: ... Blots were washed 3x with 1x TBST then incubated with ECL Prime (Amersham) for 5 minutes and imaged using a Chemidoc Imaging system (BioRad).
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bioRxiv - Neuroscience 2024Quote: ... The samples were then run on 10 % SDS-PAGE gel for 5 cm long in separation gel and stained using Bio-Safe Coomassie Stain (BioRad).
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bioRxiv - Neuroscience 2024Quote: ... Samples were then denatured for 5 min at 95°C with 50 μl of 2X sample buffer (BioRad, cat#1610737EDU) with β- mercaptoethanol ...
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bioRxiv - Neuroscience 2024Quote: ... A preliminary qPCR was then performed with a fraction of the reaction product to determine the optimal number of amplification cycles needed for a second round of amplification without saturation: to this end 5 µL of iQ SYBR Green Supermix (Biorad) were added to 5 µL preamplified DNA and used for qPCR (98°C 30 s denaturation) ...
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bioRxiv - Immunology 2024Quote: ... Membranes were washed with TBS-T (3 x 5 minutes) and incubated in horseradish peroxidase (HRP)-conjugated secondary antibody (1:1000) (BioRad) in blocking solution for 1 hour ...
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bioRxiv - Microbiology 2024Quote: ... 3-5 µL of cell suspension was then deposited on a 1.5% (w/v) low melting agarose (LMA; Bio-Rad) pad prepared using a gene frame positioned on a glass slide ...
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bioRxiv - Microbiology 2024Quote: ... boiled for 5 min at 95°C and the proteins were separated on 10–20% gradient polyacrylamide gels (Criterion precast gel, Biorad). The virus proteins were visualised by staining with Coomassie Brilliant Blue.
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bioRxiv - Cancer Biology 2024Quote: ... and RT-qPCR performed on the resulting cDNA (diluted 5-fold) using the iQ SYBR Green Supermix (Bio-Rad, #1708882) and following the manufactures instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The blots were washed again after secondary antibody incubation and developed using either nitroblue tetrazolium (NBT)–5-bromo-4-chloro-3-indolylphosphate (BCIP) for AP-conjugated secondary antibodies or the ECL substrate (BioRad) for the HRP labelled secondary antibody after incubating for about 10 to 15 mins at room temperature with gentle shaking ...
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bioRxiv - Molecular Biology 2024Quote: ... USA) in 5% (w/v) DifcoTM skim milk and developed using the ChemiDoc XRS+ imager (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Microbiology 2024Quote: ... Blots were then treated with either 1:10,000 dilutions in 5% milk/TBS-T of goat anti-mouse IgG StarBright700 (BioRad, #12004158) or goat anti-rabbit IgG IRDye800CW (Fischer ...
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bioRxiv - Microbiology 2024Quote: ... and 0.25µM of each 5’ 6-FAM/ZEN/3’ IBFQ or 5’ HEX/ZEN/3’ IBFQ probe with the QX200 Droplet Digital PCR System (Biorad). Reactions were performed using the following PCR cycle settings ...
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bioRxiv - Immunology 2024Quote: ... Membranes were then blocked with 5% non-fat milk for one hour at room temperature (Bio-Rad, Hercules, CA, USA). After blocking ...
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bioRxiv - Biochemistry 2024Quote: ... and then buffer exchanged into 150 mM ammonium acetate pH 7.5 by two consecutive passes over centrifugal gel filtration columns (Micro Bio-Spin P-6, 6-kDa cut off; BioRad). Protein concentration was verified by A280 ...
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bioRxiv - Cell Biology 2024Quote: ... Secondary antibodies were diluted in skim milk 5% in TBS-T: goat anti-mouse HRP (1:10.000, 1721011 Bio-Rad).
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bioRxiv - Cancer Biology 2024Quote: ... The calibration curve for each cytokine was analysis with 5-parametric logistic curve regression using Bio-Plex manager software (ver. 5.0, Bio-Rad). ELISAs were performed with quantification of absorbance using a spectrophotometer (BioTek) ...
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bioRxiv - Cell Biology 2024Quote: ... Digested DNA was purified and 2–5 μg of DNA was run on a 1% PFGE agarose gel (Bio-Rad) in 0.5 × TBE buffer using the CHEF-DRII system (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were washed with TBST 3 times for 5 minutes each before applying secondary fluorescent antibody (1:2,500, StarBright Blue 520 Bio-Rad) for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... Gels were run for 5 min at 130 V followed by 55min min at 180 V (PowerPac Basic Power Supply (Biorad)) ...
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bioRxiv - Cell Biology 2024Quote: ... and 5-10 μg aliquots were fractionated by SDS-PAGE and then transferred to nitrocellulose 0.45 μm membrane (Bio-Rad). Membranes were blocked for 1 h at room temperature in 5% BSA in 1x Tris-buffered saline + 0.1% Tween 20 (TBST ...
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bioRxiv - Microbiology 2020Quote: ... One or two micrograms of the concentrated viruses were heated at 95 °C for 5 min before being resolved on 4-20% SDS-PAGE (Bio-Rad) using the Novex™ Sharp Pre-stained Protein Standard (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Real time quantitative PCR (qRT-PCR) was performed using an iQ™5 Multicolor Real-Time PCR Detection System (BIO-RAD) with qPCRBIO SyGreen Mix fluorescein (PCR BIOSYSTEMS) ...
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bioRxiv - Cancer Biology 2021Quote: ... diluted in nuclease-free water and the primer(s) were incubated at 65°C for 5 minutes in the DNA Engine Dyad®Peltier Thermal Cycler (BioRad). Afterward ...
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bioRxiv - Cell Biology 2020Quote: ... to pH 8.88 first for 5 min at 95 °C followed by 60 °C for 3 h on a T100 Thermal Cycler (Bio-Rad) with the heated lid set to 105 °C ...
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bioRxiv - Cell Biology 2020Quote: ... The blots were washed four times with TBST for 5 minutes each before developing with Clarity Western ECL Blotting Substrates (Bio-Rad) and imaging ...
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bioRxiv - Immunology 2021Quote: ... They were incubated at the room temperature for 5-10 min and they were placed inside a 4-mm cuvette (Bio-Rad). The cuvettes were pulsed using the BTX electroporator (300 V ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Membranes were washed three times with TBST for 5 minutes and incubated with Goat Anti-Rabbit IgG (Bio-Rad, 170-6515) for 1 hour at RT ...
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bioRxiv - Evolutionary Biology 2021Quote: ... to 10 μL RNA and incubating at 65°C for 5 min on a C1000 Touch Thermal Cycler (Bio-Rad, USA) followed by chilling on ice ...
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bioRxiv - Developmental Biology 2020Quote: ... 50 seconds at 72°C and a final extension of 5 minutes at 72 °C in a thermal cycler (T100TM Thermal Cycler, Bio-Rad). In order to standardize the expression pattern of NvDsx ...
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bioRxiv - Biochemistry 2022Quote: ... and heated to 100 °C for 5 min and resolved on 4-20% (w/v) gradient SDS-PAGE gels (Bio-Rad) with Tris-glycine buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were then diluted 1:10 and 2 µL of diluted cDNA were used for RT-qPCR along with 5 µL of iTaq universal SYBR mix (Bio-Rad), 1.5 µL of primers (1.5 µM mix of forward and reverse ...
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bioRxiv - Molecular Biology 2021Quote: ... the supernatant was mixed with 5× RT Reaction Buffer and 10× RT Enzyme Mix and transferred to a thermal cycler (Bio-Rad). Cycling conditions were as follows ...