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Citations for Bio-Rad :
1001 - 1050 of 8842 citations for 3 Hydrazino 5 methyl 4H 1 2 4 triazol 4 ylamine hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... and 0.25µM of each 5’ 6-FAM/ZEN/3’ IBFQ or 5’ HEX/ZEN/3’ IBFQ probe with the QX200 Droplet Digital PCR System (Biorad). Reactions were performed using the following PCR cycle settings ...
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bioRxiv - Cell Biology 2021Quote: ... the membranes were washed 3 times for 5 min and incubated with the secondary antibodies (1:10000, IgG-HRP, Bio-Rad) for 45 min at RT ...
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bioRxiv - Immunology 2022Quote: ... then blocked for 1 to 3 hr at RT with 50 μL/well of blocking buffer (HN: 5% Blotting Grade Blocker, Bio-Rad cat ...
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bioRxiv - Neuroscience 2021Quote: ... of each sample was loaded on an 8-16% (for TSPO, 3b-HSD, and StAR) or 4-20% (for mERα, caveolin-1, and PKA) SDS precast gels (Bio-Rad) and separated by electrophoresis ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were incubated with primary antibodies (Supplementary Table 1) overnight at 4°C and visualised with HRP-conjugated anti-IgG secondary antibodies and enhanced chemiluminescence substrates (Bio-Rad). The expression of PDHX or calnexin was used as a loading control.
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bioRxiv - Microbiology 2020Quote: ... Clarified lysates were incubated with 2 ml of glutathione resin and rotated for 1 h at 4°C in a 25 ml disposable drip column (Bio-Rad). Settled resin was washed with 25 column volumes (CV ...
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bioRxiv - Bioengineering 2020Quote: Organoids fixed in 4% w/v PFA and immunostained were embedded in 1% w/v low melting agarose (Bio-Rad Laboratories) solution and placed in an Eppendorf tube ...
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bioRxiv - Biophysics 2020Quote: ... supplemented with DTT (0.1 M endconcentration) and then subjected to SDS-PAGE on 12% or 4–20% gradient gels (mini-PROTEAN TGX; Bio-Rad). Proteins were transferred to PVDF membranes (TransBlot® TurboTM LF PVDF ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was incubated for 1 h at 4°C with 0.5 mL Profinity IMAC resin (Bio-Rad Laboratories, Hercules, CA) in a buffer containing 20 mM MOPS-Na (pH 7.4) ...
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bioRxiv - Biochemistry 2021Quote: ... and increasing concentrations of NONO(53-312) were prepared and incubated at 4°C for 1 hour before loaded onto a Dot Blot apparatus (Bio-rad) containing a nitrocellulose membrane (top ...
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bioRxiv - Microbiology 2020Quote: The supernatants from the different lysis buffer extractions described above were diluted 1:4 in 4x Laemmli sample buffer (Bio-Rad) supplemented with beta-mercaptoethanol following manufacture’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Quantitative PCR (qPCR) was carried out using 4 μl cDNA (diluted 1:20) with iQ SYBR Green Supermix (BioRad, CA, USA) and specific primers (Biolegio ...
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bioRxiv - Molecular Biology 2023Quote: RT-qPCR was employed to find the levels of quantification of mucin 1 and 4 mRNA transcripts according to protocol of the Real-Time PCR system (BioRad./ USA)[39] ...
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bioRxiv - Molecular Biology 2024Quote: ... The membrane was then incubated overnight at 4°C with a 1:1000 concentration of anti-6×His tagged monoclonal antibody produced in mouse (ABD Serotec Biorad, UK). Then ...
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bioRxiv - Neuroscience 2024Quote: ... via wet transfer at 30V for 1 hour at 4 °C in 10% Methanol Tris/Glycine buffer using a Criterion™ Blotter (BioRad). Blots were all blocked for 1 hour at room temperature using Intercept (TBS ...
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bioRxiv - Bioengineering 2023Quote: ... Equal amounts of the proteins (50 µg) were electrophoresed (125 volts, 1 h) on a 4-20% Mini protean TGX stain-free protein gel (Bio-Rad) and transferred onto nitrocellulose membranes (125 volts ...
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bioRxiv - Physiology 2023Quote: ... Samples were run 16 h at 9 °C at 4 V with a 1–10 running ratio in TBE buffer using CHEF-DRII system (Bio-Rad). After the run ...
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bioRxiv - Microbiology 2024Quote: ... Each well was then blocked with 150 µL of blocking buffer consisting of 1×PBS + 4% (w/v) nonfat milk (Bio-Rad). The plates were blocked for 1 h at room temperature and then washed five times with PBST ...
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bioRxiv - Physiology 2024Quote: ... were diluted in 2% BSA and membrane were incubated for overnight at 4°C followed by HRP-conjugated secondary anti-rabbit antibody (1:5000, cat# 1706515, Biorad, USA) incubation for 1hr at room temperature ...
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bioRxiv - Genetics 2024Quote: ... cDNA was diluted 1:4 with water and 2ul used as template for qPCR using 250nM primers using the SsoFast EvaGreen Supermix (BioRad 1725200) on an Applied Biosystems ViiA 7 Real Time PCR System ...
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bioRxiv - Microbiology 2024Quote: ... Approximately 2-5 µg of the non-denatured protein samples were diluted 1:1 with the native sample buffer (BIO-RAD #161-0738) and loaded into the polymerized gel ...
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bioRxiv - Microbiology 2024Quote: ... 1 % 2-Mercaptoethanol (Biorad)) and bead beat for 2 minutes at maximum speed (Biospec Mini Beadbeater) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 5’-TAA TCA GAC AAG GAA CTG ATT A-3’ // Reverse: 5’-CGA AGG TGT GAC TTC CAT G-3’) and CFX96 Touch Real-Time PCR Detection System (Bio-Rad).
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bioRxiv - Microbiology 2022Quote: ... 300 nM concentration of each primer targeting the viral DNA substrate (5’- AGCGTGGGCGGGAAAATCTC-3’) and the indicated target DNA (table 1) and 1X iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories). The qPCR cycling conditions for quantifying INS activity included an initial incubation at 95°C for 3 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... and 30 μg were loaded onto a 4-15% gradient gel (Bio-Rad) for SDS-PAGE ...
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bioRxiv - Molecular Biology 2020Quote: Proteins samples were run on 4–20% Criterion TGX pre-cast gels (Biorad) in SDS/Tris-glycine running buffer and transferred to PVDF membranes by semi-dry trans-Blot Turbo system (Biorad) ...
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bioRxiv - Cell Biology 2020Quote: ... The resulting supernatants were separated on 4-15% SDS-PAGE gels (Bio-Rad) and then electro-blotted to nitrocellulose membrane using a Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: ... + βME and separated on 4–15% gradient gels (Mini-PROTEAN TGX; Bio-Rad) then transferred to nitrocellulose membrane (0.2 μm ...
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bioRxiv - Neuroscience 2021Quote: ... Concentrated protein samples (10-20μg) were separated on 7.5% or 4-15% (BioRad) SDS-PAGE gels ...
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bioRxiv - Plant Biology 2022Quote: ... 5µl was loaded into 4–20% Mini-PROTEAN® TGX Gels (BioRad, 4561096), followed by SDS-PAGE (20mA/each gel ...
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Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... Samples were loaded into precast 4-20% Tris-HCl polyacrylamide gels (Bio-Rad) with Tris/Glycine/SDS buffer (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins were resolved in a 4-15% Mini-PROTEAN TGX Precast Gels (BioRad), and transferred to a PVDF membrane using Trans-Blot Turbo Mini PVDF Transfer Packs (BioRad ...
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bioRxiv - Molecular Biology 2020Quote: ... on a 4-15% Tris-Glycine extended Stain-Free gel (Bio-Rad 5678085) at 100 V for 1.5 hrs using 1X Tris-Glycine SDS buffer (Bio-Rad 161-074) ...
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bioRxiv - Microbiology 2020Quote: ... 40 µg tissue lysate was run on a 4-20% acrylamide gel (Biorad).
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bioRxiv - Biochemistry 2020Quote: ... Samples were loaded onto a gradient 4-20% native PAGE gel (Bio-Rad), electrophoresed at 180V for ∼1hr in 0°C native running buffer (25 mM Tris ...
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bioRxiv - Immunology 2020Quote: ... Proteins were resolved on a 4-20% Mini Protean TGX gradient gel (BioRad), and transferred onto PVDF membrane ...
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bioRxiv - Plant Biology 2021Quote: ... Samples were loaded and run on a 4 - 20 % precast polyacrylamide gel (BioRad, 4561094 ...
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bioRxiv - Plant Biology 2020Quote: ... The gels ran at 4 °C in a Mini-PROTEAN Tetra System (BioRad) at 35 mA per gel for 60 min ...
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bioRxiv - Plant Biology 2020Quote: ... Electrophoresis was performed at 4 °C in a Mini-PROTEAN Tetra System (BioRad) at 100 V for 30 min with Blue Cathode Buffer (15 mM BisTris-HCl ...
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bioRxiv - Plant Biology 2020Quote: ... RT-qPCR reaction was performed in a Chromo 4™ system cycler (Biorad). Four technical replicates were produced per plant by repeating the cDNA synthesis step separately twice and performing two RT-qPCR reactions per cDNA sample ...
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bioRxiv - Neuroscience 2020Quote: ... or 4-20% Criterion™ TGX Stain-Free™ Protein gels (Bio-Rad) and transferred to 0.2 μm nitrocellulose membranes (10600002 ...
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bioRxiv - Bioengineering 2021Quote: ... were loaded onto 4-20% Mini-PROTEAN TGX Precast Protein Gels (BioRad, 4561093). The separated proteins were transferred to a PVDF membrane (BioRad ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were loaded on a 4-to-20% SDS polyacrylamide gel (Bio-Rad). Membranes were blocked for 3 hr in 5% milk in TBS (50 mM Trizma base and 150 mM NaCl ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lysates were run in 4-20% gradient SDS-PAGE gels (Bio-Rad) (5×106 cells/ lane ...
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bioRxiv - Cell Biology 2021Quote: Proteins were resolved on 4–20% Criterion TGX precast gels (Biorad, cat #5671095) running at 200V for 40 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatants were loaded onto a 4-20% protein gel (Bio-Rad #4568096) for SDS-PAGE analysis ...
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bioRxiv - Biochemistry 2021Quote: ... Samples were run twice on a Criterion XT 4-12% polyacrylamide gel (Biorad) in MES XT buffer (Biorad ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were separated by SDS-PAGE in 4 – 15 % gradient gels (TGX, Biorad) and 32P-derived signals were visualized by exposing the gel to an X-ray film (SuperRX ...
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bioRxiv - Plant Biology 2020Quote: ... for 16 h at 4°C using a wet transfer system from BioRad (transfer buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... before being electrophoresed through a 4-12% NuPAGE Bis-Tris polyacrylamide gel (BioRad). Protein bands were then transferred from the gels onto 0.2 μm polyvinylidene fluoride (PVDF ...