Labshake search
Citations for Bio-Rad :
951 - 1000 of 1047 citations for Recombinant Mouse Folh1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2024Quote: ... blots were washed three times in TBST for 5 minutes each and incubated with an anti-mouse HRP secondary (Bio-Rad) at 1:2000 dilution for 1 hour at room temperature ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... we used the multiplex bead array system Bio-Plex Pro Mouse Chemokine Assay Panel 31-Plex (#12009159, Bio-Rad Laboratories GmbH), according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2024Quote: Total RNA was extracted from mouse tissues using the Aurum Total RNA Fatty and Fibrous Tissue Extraction kit (Biorad: #732-6870). RNA from cells was extracted using Zymo Research R1055 Quick-RNA MiniPrep Kit ...
-
bioRxiv - Neuroscience 2023Quote: ... Blots were washed thrice and incubated with a secondary antibody (Goat anti-Mouse IgG-HRP, 1706516, Bio-Rad Laboratories, 1:10K), also diluted in 2.5% milk in TBST ...
-
bioRxiv - Cell Biology 2024Quote: ... Membranes were washed three times in PBS + 0.05% Tween-20 (PBS-T) and incubated in goat anti-mouse or goat anti-rabbit horseradish peroxidase (HRP)-conjugated secondary antibody (Bio-rad) diluted 1:5000 in blocking solution ...
-
bioRxiv - Immunology 2024Quote: ... Membranes were washed then incubated for 1 hour at room temperature with secondary antibody (Goat Anti-Mouse Starbright Blue 520 and Goat Anti-Rabbit Starbright Blue 700, each at 1:2500, Bio-Rad). Blots were analyzed (ChemiDocTM MP Imaging System ...
-
bioRxiv - Immunology 2024Quote: ... tissue sections were blocked in 10% normal goat serum for 1 h at room temperature and incubated in rat anti-mouse CD4 (1:200; MCA2691; Bio-Rad) or CD8a (Biolegend ...
-
bioRxiv - Immunology 2021Quote: ... The monolayer was fixed with 4% paraformaldehyde and permeabilized with Triton-X100 and stained with mouse anti-NP IgG1 (clone AA5H, Bio-Rad Antibodies) followed by goat anti-mouse HRP (DAKO ...
-
bioRxiv - Molecular Biology 2019Quote: ... The blot was washed 4 times with TBS-T and subsequently incubated with a goat anti-mouse-HRP antibody (Bio-Rad #1706516) for 30 minutes ...
-
bioRxiv - Developmental Biology 2019Quote: ... Subsequent analysis was conducted on a 12% SDS-PAGE gel and blots were probed with either mouse α-V5 (SV5-Pk1, 1:1000, Bio-Rad), mouse α-HA (HA.11 ...
-
bioRxiv - Neuroscience 2021Quote: ... The blotted membranes were rinsed three times with 0.05% Tween 20 in Tris-HCl-buffered saline (TBST) and incubated with horseradish peroxidase-conjugated goat polyclonal antibody against mouse IgG (Bio-Rad Laboratories) or guinea pig IgG (Bioss Inc. ...
-
bioRxiv - Cancer Biology 2021Quote: ... membranes were washed 3x with TBS-T and incubated with either Goat Anti-Mouse IgG (H+L)-HRP Conjugate (Bio-Rad, #1721011) or Goat Anti-Rabbit IgG (H+L)-HRP Conjugate (Bio-Rad ...
-
bioRxiv - Cell Biology 2021Quote: ... The membrane was washed three times for 10 minutes with 1x PBS and incubated with Goat Anti-Mouse IgG (H+L)-HRP Conjugate (BioRad, #172-1011). Chemiluminescence was induced by incubation with SuperSignal West Femto Maximum Sensitivity Substrate (ThermoFisher ...
-
bioRxiv - Microbiology 2021Quote: Bio-Plex assays for cytokines and chemokines were carried out in Bio-Plex 200 system using Bio-Plex Pro Mouse Cytokine Grp I Panel 23-Plex assay kit (Bio-Rad, M60009RDPD) following manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2020Quote: ... Secondary antibodies used for Western blotting were: Donkey anti-mouse- and anti-rabbit-HRP (#172-1011 and #170-6515, Bio-Rad, USA). Secondary antibodies used for immunofluorescence were ...
-
bioRxiv - Microbiology 2022Quote: ... rabbit polyclonal anti-nsP1 (both validated in our laboratory) [11] and mouse monoclonal anti-GAPDH (Cat. # VMA00046, Bio-Rad, Hercules, CA, USA). Then ...
-
bioRxiv - Bioengineering 2022Quote: ... The PVDF membrane was then washed 3 × 10 min with TBST and incubated with horseradish peroxidase (HRP)-conjugated goat anti-mouse (diluted 1:20,000; Bio-Rad, Hercules, CA) secondary antibody for one hour at room temperature followed by 6 × 10 min washes with TBST and detection using the Radiance Plus chemiluminescence substrate (Azure Biosystems ...
-
bioRxiv - Cancer Biology 2022Quote: ... slides were washed twice with PBS and incubated with secondary antibodies (Desmin and MYOD1: anti-rabbit-HRP, BioRad 170-6515; Myogenin: anti-mouse HRP, BioRad 170-6516) 1:500 in PBS-1% BSA ...
-
bioRxiv - Microbiology 2022Quote: ... cells were heat-fixated in 96-well plates and a sandwich assay with NP-specific monoclonal antibody H16-L10-4R5 (www.atcc.org) and a peroxidase-coupled goat anti-mouse IgG HRP polyclonal antibody (Bio-Rad Laboratories GmbH, Germany) was carried out ...
-
bioRxiv - Microbiology 2021Quote: ... The purity of the eluted fractions was assessed by SDS-PAGE and Western blot using a mouse anti-histidine antibody (BioRad, AbD Serotec).
-
bioRxiv - Cancer Biology 2022Quote: ... Blots were incubated in secondary antibody for one hour at room temperature (anti-rabbit; Amersham, #NA934) (anti-mouse; Amersham, #NA931) and visualized with Clarity Western ECL Substrate (Bio-Rad, #1705061) on a ChemiDoc Imaging System (Bio-Rad).
-
bioRxiv - Developmental Biology 2021Quote: ... Five hundred ng of total RNA (from 21EM15 cells or mouse lenses) or 10 ng of total RNA (from explants) were reverse transcribed using the iScript cDNa Synthesis Kit (Bio-Rad, CA) per the manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... equal protein amounts of the lysates (25 μg) were applied to the Bio-Plex Mouse Cytokine 23-plex Assay Kit (Bio-Rad Laboratories) and measured with the BioPlex200 system and BioPlex-Manager 6.2 according to manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... Secondary antibodies used for Western blotting were: Goat anti-mouse- and anti-rabbit-HRP (#172-1011 and #170-6515, Bio-Rad, USA).
-
bioRxiv - Cancer Biology 2022Quote: ... membranes were incubated with complementary HRP conjugated secondary antibody (sheep anti-mouse IgG-HRP, 1:10000, GE Healthcare, NA931; goat anti-rabbit IgG-HRP, 1:5000, Bio-Rad, 1706515 ...
-
bioRxiv - Immunology 2022Quote: ... To each well 50 µL of 2 µg/mL of biotin-conjugated mouse anti-bovine IFN-γ mAb (CC302, Bio-Rad Antibodies) diluted in PBS was added and plates incubated for 2 hours at RT ...
-
bioRxiv - Microbiology 2022Quote: ... Membranes were rinsed 3x for 15 minutes in 1X TBST and incubated in the proper secondary antibody (Goat anti-mouse BioRad #170-6516 or Goat anti-rabbit BioRad #170-6515) for 1 hour ...
-
bioRxiv - Physiology 2022Quote: Maternal plasma and SI IEL proteins were assayed for cytokine levels according to manufacturer’s instructions using the Bio-Plex Pro Mouse Cytokine 23-Plex Assay (Bio-Rad, Mississauga, Canada) and the Luminex system (Bio-Rad ...
-
bioRxiv - Immunology 2023Quote: ... Cytokines were assayed through using Bio-Plex Pro-Mouse Group I Cytokine 23-plex assay kit (M60009RDPD, Bio-Rad, Hercules, California, USA) and the Bio-Plex® 200 System ...
-
bioRxiv - Immunology 2023Quote: ... Supernatants were collected following centrifugation of the homogenate at 18,000 gravity for 20 minutes at 4°C and then stored at -80°C until analysis by Bio-Plex Pro Mouse Cytokine 23-plex Assay (Bio-Rad Laboratories) following the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2022Quote: ... Secondary antibodies used for Western blotting were: Goat anti-mouse- and anti-rabbit-HRP (#172-1011 and #170-6515, Bio-Rad, USA).
-
bioRxiv - Cell Biology 2022Quote: ... The proteins were detected using chemiluminescence using HRP conjugated secondary antibodies of either anti-mouse (Bio-Rad # 170-6516, dilution 1:10,000) or anti-rabbit (GE Healthcare # NA9340 ...
-
bioRxiv - Microbiology 2023Quote: ... Heart homogenates were analyzed for cytokine and chemokine levels using a Bio-Plex Pro Mouse Cytokine 23-plex Assay kit (Bio-Rad, #m60009rdpd) and ProcartaPlex mouse IFN-α/IFN-β 2-plex (ThermoFisher ...
-
bioRxiv - Neuroscience 2023Quote: ... The blot was washed 3 times with TBST for 5 minutes and was incubated with 10 mL of 3% BSA/TBST (w/v) with 1 µL anti-mouse-HRP secondary antibody (1:10,000; Bio-Rad, 170-6516) for 30 minutes at room temperature ...
-
bioRxiv - Microbiology 2023Quote: ... slides were incubated for 30 min at room temperature with primary mouse monoclonal anti-feline coronavirus antibody at 1:400 (BioRad, MCA 2194). The EnVision anti-mouse system was used for visualization according to the manufacturer’s instructions (Agilent).
-
bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies used for Western blotting were goat anti-mouse- and anti-rabbit-HRP (#172-1011 and #170-6515, Bio-Rad, USA).
-
bioRxiv - Genetics 2023Quote: ... 5µL of urine per mouse was loaded on a 4–15% Mini-PROTEAN TGX Stain- Free Gel (Bio-Rad, Hercules, CA, USA) before Coomassie staining with QC Colloidal Coomassie (Bio-Rad ...
-
bioRxiv - Molecular Biology 2024Quote: ... Membranes were washed prior to incubation with a goat anti-mouse StarBright™ Blue 700 secondary (Catalogue #: 12004158; Bio-Rad, Hercules, CA). Gag expression levels were normalized relative to GAPDH levels ...
-
bioRxiv - Microbiology 2024Quote: ... the samples were prepared as instructed by the manufacturer and read using Bio-Plex Pro Mouse Cytokine 23-Plex (Bio-Rad, Hercules). Samples were run in technical duplicates ...
-
bioRxiv - Cell Biology 2024Quote: ... The proteins were detected using chemiluminescence using horseradish peroxidase–conjugated secondary antibodies of anti-mouse (Bio-Rad # 170-6516, dilution 1:10,000) origin and enhanced chemiluminescence substrate (Thermo Scientific # 32106) ...
-
bioRxiv - Cell Biology 2020Quote: ... followed by 1◻h incubation at 4◻°C with HRP-conjugated goat anti-mouse (1:5000 dilution in 1% YE in PBS-T, BioRad #170–6516) secondary antibody ...
-
bioRxiv - Neuroscience 2021Quote: ... PAI-1 and insulin) from plasma samples were analyzed by duplicate using the Bio-PlexPro mouse Diabetes group from Bio-Rad (Ref. 171F7001M) by Luminex® 200TM technology as previously described (Ortega Moreno et al. ...
-
bioRxiv - Plant Biology 2021Quote: ... The following day it was probed with 1:2000 anti-Myc primary antibody (rabbit polyclonal, Santa-Cruz, A14) and with 1:10000 mouse anti-rabbit HRP linked secondary antibody (Bio-Rad, 170-6515). Amersham ECL prime HRP substrate (GE-Lifesciences ...
-
bioRxiv - Neuroscience 2020Quote: ... Diabetes-associated markers and cytokine levels in the plasma were assessed using the Bio-Plex Pro Mouse Diabetes 8-Plex Assay (Cat# 171F7001M; Bio-Rad, Carlsbad, California) and the Bio-Plex Pro Mouse Cytokine 23-Plex Assay (Cat# M60009RDPD ...
-
bioRxiv - Developmental Biology 2019Quote: ... and incubated overnight at 4° with primary antibodies diluted in blocking solution as follows: mouse α-V5 (1:1000, SV5-Pk1, Bio-Rad #MCA1360); mouse α-SP56 (1:200 ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: The level of proinflammatory mediators in plasma (50 μL) was measured with the help of Bio-Plex Pro Mouse Cytokine Standard 23-Plex (Cat# 64209360, Bio-Rad, Hercules, CA) as per manufacturer’s protocol ...
-
bioRxiv - Microbiology 2021Quote: ... 1:5000; # HAF017, R&D Systems, goat anti-rabbit, 1:5000; #170-6515, Bio Rad, goat anti-mouse, 1:5000; #6516, Bio-Rad). To ensure that equal amounts of proteins were loaded ...
-
bioRxiv - Developmental Biology 2019Quote: Protein isolated from placental homogenates were assayed for cytokine levels according to manufacturer’s instructions using the Bio-Plex Pro Mouse Cytokine 23-Plex Assay (Bio-Rad, Hercules, CA, USA) and the Luminex system (Bio-Rad ...
-
bioRxiv - Physiology 2020Quote: ... The following antibodies were selected and tested on half of each cornea: anti-CD31 mouse monoclonal IgG1 targeting vascular endothelial cells (BioRad, Kidlington, Oxford, England), anti-Neutrophil rabbit polyclonal IgG targeting neutrophils (LifeSpan BioSciences ...
-
bioRxiv - Cell Biology 2020Quote: ... followed by a 1.5 h incubation with the respective secondary antibodies: StarBright700 anti-rabbit (10000068187, 1:5000) or StarBright700 anti-mouse (10000068185, 1:2500) both from BioRad (Hercules, CA, USA). Four washes with TBS-T were performed after incubation with secondary antibodies ...