Labshake search
Citations for Bio-Rad :
951 - 1000 of 2250 citations for Mouse Anti Nipah Virus Glycoprotein G JB3 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: Purified substrates and enzymes were centrifuged (16,000 g, 5 min, 4°C) before protein concentration was determined by Bradford assay (Bio-Rad) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2020Quote: ... 10μg/μl of protein was loaded into each lane of 4–15% (mg/100 mL) Tris·HCl polyacryamide gels (Bio-Rad). Protein was transferred to Immobilon-P PVDF membranes (Millipore) ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... The suspension was centrifuged for 30 min at 24,000 g and the supernatant was loaded onto a column packed with 1g of dry hydroxyapatite (Bio-Rad). After elution with extraction buffer the fractions collected containing proteins were diluted in 20 mM MES buffer ...
-
bioRxiv - Immunology 2020Quote: ... first Protein A/G agarose was loaded on Micro Bio-Spin™ Chromatography Columns (Bio-Rad Laboratories, Hercules, CA, USA). After gravity flow ...
-
bioRxiv - Cell Biology 2021Quote: ... Samples were centrifuged at 13 000 g for 10 min and resolved using SDS-PAGE gels and transferred onto Polyvinylidene difluoride membranes (PVDF; BioRad). The membranes were blocked with 5% milk in TBST and probed with the indicated primary antibodies in 5% BSA/TBST and a 1:10000 dilution of anti-rabbit or anti-mouse horseradish peroxidase-conjugated secondary antibodies in 5% BSA/TBST ...
-
bioRxiv - Immunology 2022Quote: ... Lysates containing 40 μg of proteins were analyzed by SDS-PAGE on 9-13% gels and proteins were transferred onto nitrocellulose membranes (BioRad). The blots were blocked in non-fat milk diluted in tris-buffered saline (TBS ...
-
bioRxiv - Molecular Biology 2022Quote: ... 5 μg of ssDNA were added to 95 μl of VSV-G-EVs in trehalose buffer and EVs then transferred to a 1 mm electroporation cuvette (BioRad) placed on ice ...
-
bioRxiv - Genetics 2022Quote: ... the antibodies were prepared by mixing 3 ug of antibody HNRNPK and IgG (as a negative control in a separate tube) with 650 ul of Dilution Buffer and 50 ul of Surebeads Protein G magnetic Beads (BioRad) and left in rotation for 30 minutes at 4 °C ...
-
bioRxiv - Neuroscience 2022Quote: ... equal amounts of protein (2.5 or 10 µg) were separated by SDS-PAGE on 4-12% Bis-Tris or 3-8% Tris acetate gels (Criterion XT, BioRad) and transferred to nitrocellulose using TransBlot Turbo (BioRad) ...
-
bioRxiv - Neuroscience 2022Quote: ... Regional lysates were centrifuged at 21,000 x g for 20 minutes at 4 °C and the supernatant was removed and suspended in 4X laemmli buffer (BioRad) with 2-mercaptaethanol (BioRad ...
-
bioRxiv - Immunology 2023Quote: ... 15 to 30 µg of protein sample was processed for separation through SDS PAGE on 4–20% Criterion TGX precast gradient gels (BioRad). Gel run consisted of 15 min run at 70V followed by 45 min run at 100V ...
-
bioRxiv - Cancer Biology 2023Quote: ... Lysates were cleared by centrifugation at 14,000 g for 15 min at 4°C and protein concentration was determined using the Bradford assay (Bio-Rad). Sodium dodecyl sulfate (SDS ...
-
bioRxiv - Cancer Biology 2023Quote: ... 30-50 µg of nuclear proteins were loaded onto a 10% SDS-PAGE and transferred on nitrocellulose membrane using TransBlot apparatus (BioRad). Membranes were subsequently incubated with TBS Tween-20® 0.01% ...
-
bioRxiv - Genetics 2023Quote: ... Total protein (75 μg per lane) was loaded onto pre-cast 4-20% gradient Mini-PROTEAN TGX Stain-Free SDS PAGE gels (BioRad). Proteins were transferred onto nitrocellulose membrane (0.2 μm pore size ...
-
bioRxiv - Physiology 2023Quote: ... and left ventricle of wild-type C57 Bl6 mice was incubated with PC2 antibody (10 ug, Alomone laboratories) overnight complexed to magnetic protein G beads (Biorad). Following 3 rounds of washing ...
-
bioRxiv - Biochemistry 2023Quote: ... actin monomers in G-buffer were incubated with AG1-X2 100–200 mesh anion exchange resin (Dowex; 1401241; Bio-Rad) at 4 °C for 5 min to remove ATP ...
-
bioRxiv - Biochemistry 2023Quote: ... The lysates were pelleted at 10,000 g for 10 min and the supernatants were used to determine the protein concentration using Bradford assay (Bio-Rad). After a preclearing step with Protein G Sepharose (Sigma) ...
-
bioRxiv - Microbiology 2023Quote: ... 10 min), centrifuged (1,000 x g, 2 min) and loaded on 10% or 4–20% SDS-PAGE gel (Bio-Rad). Following electrophoresis (60 min ...
-
bioRxiv - Microbiology 2023Quote: ... followed by immunoprecipitation with 5 µg appropriate antibodies or corresponding IgG control using magnetic protein A/G beads (BIO-RAD). After several rounds of washing with low-salt wash buffer (20 mM Tris-HCl ...
-
bioRxiv - Microbiology 2023Quote: Protein samples were centrifuged (150,000 × g for 10 min at 4°C) and loaded onto an Enrich SEC 650 10 × 300 column (Bio-Rad) using the AKTA explore system (GE Healthcare ...
-
bioRxiv - Cell Biology 2023Quote: ... actin monomers in G-buffer were incubated with AG1-X2 100–200 mesh anion exchange resin (Dowex; 1401241; Bio-Rad) at 4 °C for 5 min to remove ATP ...
-
bioRxiv - Biochemistry 2023Quote: ... It was first clarified by centrifugation at 10,000 g for 30 min and loaded onto a 10-mL self-assembled Affi-Gel Blue gravity column (Bio-Rad). The column was sequentially washed with 50 mL resuspension buffer containing 0.3 M NaCl ...
-
bioRxiv - Bioengineering 2024Quote: ... The gels were run for 55-minutes at 150V and stained with G-250 Coomassie stain (Bio-Rad, Cat# 1610803). Final quantified protein yield was determined by gel band intensity measurements using Fiji (https://fiji.sc/).
-
bioRxiv - Molecular Biology 2021Quote: ... mouse-HRP and rabbit-HRP secondary antibodies were purchased from BioRad Laboratories (Hercules ...
-
bioRxiv - Genomics 2020Quote: ... and Goat α-mouse secondary (Bio-Rad Laboratories, Hercules, CA, 1706516) at a 1:5,000 dilution were used within milk as blocking buffer ...
-
bioRxiv - Developmental Biology 2019Quote: ... mouse α-V5 (SV5-Pk1, 1:1000, Bio-Rad, Hercules, CA), mouse α-SP56 (1:200 ...
-
bioRxiv - Cancer Biology 2019Quote: ... Mouse cytokine 23-plex immunoassay (Bio-Plex®; Bio-Rad Laboratories) and chromogenic sandwich enzyme-linked immunosorbent assay (ELISA ...
-
bioRxiv - Immunology 2020Quote: ... or Pro-Mouse cytokine BioPlex® 200 multiplex (Bio-Rad Laboratories) according to the manufacturer’s instructions.
-
bioRxiv - Molecular Biology 2021Quote: ... FAM; dMmuCPE5121630) and Hsp90ab1 (Mouse; PrimePCR ddPCR Expression Probe Assay (BioRad); HEX ...
-
bioRxiv - Neuroscience 2022Quote: ... Secondary antibodies were goat α-mouse IgG-HRP (Biorad, 1706516; RRID:AB_11125547), goat α-mouse IgG2b-HRP (Invitrogen ...
-
bioRxiv - Pathology 2023Quote: ... or mouse monoclonal antibodies against human C3d (Bio-Rad Laboratories, CA) and C4d (Santa Cruz ...
-
bioRxiv - Pathology 2023Quote: ... Bio-Plex Pro Mouse Cytokine Panel 33-Plex (Bio-Rad, USA) was used to measure the levels of chemokines and cytokines ...
-
bioRxiv - Neuroscience 2024Quote: ... Primers/probes to detect mouse Pcbp2 (BioRad Custom Assay, Hercules, CA) were used as an endogenous reference in mouse samples ...
-
bioRxiv - Cell Biology 2020Quote: ... were added to this for a further 4 h before samples were centrifuged at 10,000 x g for 3 minutes and washed three times with RIPA buffer and re-suspended in laemmli buffer (Bio-Rad), boiled for 5 minutes ...
-
bioRxiv - Developmental Biology 2020Quote: ... 1 µg of total RNA and oligo (dT) primers were reverse transcribed using the iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad). RT-PCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad ...
-
bioRxiv - Developmental Biology 2021Quote: ... 15µg of each sample was loaded per lane of a MIDI 4-15% Criterion Precast Midi SDS page gel (BioRad #5671085) and electrophoresis was performed at 120mV for 75 minutes ...
-
bioRxiv - Molecular Biology 2021Quote: ... and the equivalent of 50 μg protein input (elution) were analyzed on a 4%-12% gradient XT precast Criterion gel using XT-MOPS buffer (Bio-Rad).
-
bioRxiv - Biophysics 2022Quote: ... 0.5 μg of total RNA sample (100 ng/μl) was retrotranscribed in stable cDNA with iScriptTM Advanced cDNA Synthesis Kit (Bio-Rad). Genes of interest were amplified with Itaq UniversSYBR Green (Bio-Rad) ...
-
bioRxiv - Molecular Biology 2021Quote: ... The concentration of purified proteins was determined using the Bradford assay and purity was verified by electrophoresis in sodium dodecyl sulfate (SDS)-polyacrylamide gel which was subsequently stained using Bio-Safe Coomassie G 250 (Bio-Rad).
-
bioRxiv - Molecular Biology 2019Quote: ... 5-15 µg of protein was resolved by SDS/PAGE and transferred to nitrocellulose membranes using the Trans-Blot Turbo System (Bio-Rad). Membranes were blocked in 5% milk in phosphate buffered saline with 0.1% Tween (PBS-T ...
-
bioRxiv - Biophysics 2021Quote: ... The mixture was diluted to ~30 mL with sample buffer and stirred at 4°C for 30 min prior removal of C12E8 by adding 4 g of Bio-Beads SM-2 (Bio-Rad) in stages of 0.5 ...
-
bioRxiv - Biochemistry 2021Quote: ... 10 μg of total protein was run on a 4–15% SDS-PAGE gel (Mini-PROTEAN TGX Precast Protein Gel; Bio-Rad). Proteins were then transferred onto a PVDF membrane using a Trans-Blot® SD Semi-Dry Transfer Cell (Bio-Rad ...
-
bioRxiv - Pathology 2021Quote: ... maximum power with 30 second intervals and the lysate obtained by ultracentrifugation at 100,000 g for 60 minutes, 4°C (CS 100FNX, HITACHI) resuspended in PBS and the concentration was measured by Bio-Rad protein assay dye reagent concentrate (BioRad) ...
-
bioRxiv - Cancer Biology 2021Quote: ... were run on a 1 mm thick 10% SDS-PAGE gel and stained with CBB G-250 staining solution (Bio-Rad) at RT for 1.5 h ...
-
bioRxiv - Cancer Biology 2020Quote: ... 30 μg of protein was loaded into each lane of a TGX-stain-free pre-cast 4–20% SDS gel (Biorad, 4568094), total protein was quantified upon stain-free gel imaging ...
-
bioRxiv - Cell Biology 2021Quote: ... The supernatant was collected after centrifugation at 15,000 ×g for 15 min at 4°C and applied to a 20 mL open column (Poly-Prep Chromatography Columns; BIO-RAD) containing 500 µL of Ni-NTA agarose (Qiagen ...
-
bioRxiv - Cell Biology 2020Quote: ... Triton-insoluble material was then pelleted at 15890 x g for 10 min and protein concentration measured with the DC assay (Bio-rad). For immunoblot analysis ...
-
bioRxiv - Cell Biology 2022Quote: ... lysates were then clarified through centrifugation (14’000 g, 20 minutes, 4°C) and then quantified using Bradford Reagent (Bio-Rad Laboratories) according to manufacturer’s instructions.
-
bioRxiv - Plant Biology 2022Quote: ... The extract was centrifuged at 18,000 g for 10 minutes at 4 °C and the protein content of the supernatant determined using the Bradford method (Bio-Rad) in a spectrophotometer measuring the absorbance at 595 nm and then eluted in Laemmli 2X buffer with 8 % β-mercaptoethanol (95 °C for 5 minutes) ...
-
bioRxiv - Neuroscience 2022Quote: ... Used nest building material was removed from the cages and replaced by 11 g compressed extra-thick blot filter paper (Bio-rad). Every 24h for 5 days ...