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Citations for Bio-Rad :
951 - 1000 of 4663 citations for 7 BROMO 1 METHYL 1H QUINOXALIN 2 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: One hundred nanograms of total RNA was reverse-transcribed (RT) and amplified using the iScript One-Step RT-PCR kit for probes (Bio-Rad, Hercules, CA). Real-time qPCR was performed with the Bio-Rad CFX96 sequence detection system using predesigned primer/probe sets against CPT1a ...
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bioRxiv - Microbiology 2023Quote: ... RNA reverse transcription and PCR amplification were performed in a single reaction using the One-Step RT-ddPCR Advanced Kit for Probes (BioRad, Hercules, CA, USA) per the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... to allow detection of both in one reaction droplet ddPCR was performed with a BioRad QX100 ddPCR machine using the recommended protocols and reagents (BioRad Labatories, Hercules, CA). ddPCR was performed on biological quadruplicates of males and females ...
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bioRxiv - Microbiology 2024Quote: ... followed by real-time RT-PCR targeting highly conserved regions of the IAV matrix (M) (10) using iTaq Universal Probes One-Step Kit (Bio-Rad, 1725141, USA) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were electrophoresed in a Bio-Rad Criterion TM Vertical Electrophoresis Cell at 90-120V for 1-2 hours and then transferred to a PVDF Transfer Membrane (#88518, Bio-Rad, Hercules, CA, United States) using the Bio-Rad Trans-Blot Turbo Transfer System ...
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bioRxiv - Plant Biology 2021Quote: ... the Agrobacterium-infiltrated leaves were visually inspected at 2-5 days post infiltration and/or the fluorescence of the infiltrated leaves were monitored at 1-2 d post infiltration under the Pro-Q Emerald 488 in the ChemiDoc™ Gel Imaging System (Bio-Rad, Hercules, CA, USA) as described previously (Yoon and Rikkerink 2020).
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bioRxiv - Neuroscience 2021Quote: ... the membranes were incubated for 2 h at room temperature in HRP conjugated IgG anti-rabbit secondary antibody (Bio-Rad 170-65-15, 1:3000) or Cy5 conjugated IgG anti-mouse secondary antibody (Jackson ImmunoResearch Laboratories Europe Ltd. ...
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bioRxiv - Microbiology 2019Quote: ... for 5 min in 2 ml screw-cap vials with 2 g of glass beads (2mm) and 200 μl of buffer containing 1 × TE buffer and Chelex®-100 (Bio-Rad Laboratories, CA, USA). Samples were then incubated overnight at 56 °C with 10 μl of Proteinase K (20mg/ml ...
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bioRxiv - Neuroscience 2023Quote: ... sections were blocked in serum and then incubated in primary antibody for 2 hr at RT (CD-68: 1:500, Bio-Rad clone FA-11, MCA1957GA), and then processed for secondary and HRP reagent using a Vectastain ABC kit according to the manufacturer’s instructions (Rat IgG ...
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bioRxiv - Microbiology 2021Quote: ... was used to detect and quantify SARS-CoV-2 RNA using the SARS-CoV-2 Droplet Digital PCR Kit (Bio-Rad), which contains a triplex assay of primers/probes aligned to the CDC markers for SARS-CoV-2 N1 and N2 genes and human RPP30 gene ...
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bioRxiv - Biochemistry 2019Quote: ... was transferred to a new tube with 50 μL 2 M HCl and boiled for 2 hours at 95°C in a C1000 thermocycler (Bio-Rad) to hydrolyze all glucans to glucose ...
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bioRxiv - Microbiology 2022Quote: ... 2×2 mm squares were cut out and placed into double-sided sticky 9×9 mm frame seals (Bio-Rad SLF0201) on a glass slide ...
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Targeting MOG to skin macrophages prevents EAE in macaques through TGFβ-induced peripheral tolerancebioRxiv - Immunology 2019Quote: ... TGFβ2 and TGFβ3), as 2 separate dosages, following supplier (Merckmillipore, Burlington, MA, USA) guidelines using a Bioplex 200 (BioRad, Hercules, CA, USA). The quantification of all samples was performed all together.
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bioRxiv - Bioengineering 2022Quote: ... pH 8.3 at 100 V for 2 h at 2-8 °C using PowerPac Basic setup (Bio-Rad Laboratories Inc., Hercules, CA). Post-electrophoresis ...
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bioRxiv - Immunology 2024Quote: ... Cells were then resuspended in RPMI complete media at 1×106 cells/ml and stimulated with 5 µg/ml of anti-Igκ plus anti-Igλ F(ab’)2 antibodies (BioRad, Table 2) for the indicated time at 37°C ...
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bioRxiv - Biophysics 2022Quote: ... Experiments were performed in triplicate and densitometry was carried out using a DNA standard curve and Quantity One® software (Bio-Rad, Hercules, CA). The values reported in Figure 2 were calculated by dividing the portion of Oct4-nucleosome complex by the value of starting free nucleosome (control) ...
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bioRxiv - Molecular Biology 2020Quote: ... Chemiluminescence was detected with an ImageQuant Las 4000 instrument (Cytiva, Little Chalfont, UK) and quantified using Bio-Rad Quantity One software (Bio-Rad, Hercules, CA, USA). For a reference standard we used an in-house produced monoclonal antibody of known concentration.
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bioRxiv - Developmental Biology 2020Quote: ... and one-dimensional electrophoresis was carried out for 4 hours at 75-100 volts in a Tris-glycine-SDS (Bio-Rad, catalog 161-0732) running buffer ...
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bioRxiv - Microbiology 2021Quote: The 20 μl RT-dPCR reaction mixture consisted of 5 μl One-Step RT-dPCR Advanced Supermix for Probes (Bio-Rad, Laboratories, Munich, Germany), 2 µl of Reverse Transcriptase (Bio-Rad ...
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bioRxiv - Cancer Biology 2020Quote: ... One μg RNA was reverse-transcribed using oligo(dT)18 and iScript Advanced cDNA synthesis kit for RT-qPCR (Bio-Rad Laboratories; Hercules, CA). Il1r1/IL1R1 and Gapdh/GAPDH qPCR was performed using specific primers and Lightcycler 480 Sybr Green I Master (Roche ...
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bioRxiv - Microbiology 2021Quote: ... The amount of viral RNA expressed as TCID50 equivalent per insert (TCID50e/insert) was quantified by RT-qPCR using iTaq universal probes one-step kit (Bio-Rad, catalogue no. 1725141), and a commercial mix of primers for N gene (forward primer 5’-GACCCCAAAATCAGCGAAAT-3’ ...
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bioRxiv - Biochemistry 2022Quote: ... the HRP signals were visualized by using Chemi-Lumi One Super (02230-30, Nacalai Tesque) and a ChemiDocTM Imaging System (Bio-Rad Laboratories, Hercules, CA). The primary antibodies used in this study were anti-β-actin antibody (M177-3 ...
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bioRxiv - Microbiology 2020Quote: ... IL-22 and TNF-α was analyzed by using quantitative RT-PCR (qRT-PCR) according to the Biorad iScript™ One-Step RT-PCR Kit in a CFX96 apparatus (Bio-Rad, Hercules, CA) with the following primers ...
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bioRxiv - Cell Biology 2021Quote: ... or mRNA isolated from cell lines or primary mouse cells were proceeded to RT-qPCR using iTaq universal SYBR green one step kit (Bio-Rad, Cat. No. 1725150). DNA obtained from ChIP was proceed to RT-qPCR using iQ SYBR green supermix (Bio-Rad ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... ECL substrate was added according to manufacturer’s instructions after which chemiluminescence was analyzed in a Molecular Imager ChemiDoc XRS by Quantity One Software (Bio-Rad Laboratories, Hercules, CA, USA).
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bioRxiv - Cancer Biology 2020Quote: The ddPCRs assay comprised a total volume of 20 μL containing on the one hand the droplet Supermix (Bio-Rad Laboratories, Richmond, CA, USA) at final concentration of 500 nM of forward and reverse primers and 250 nM of VIC- and FAM-labeled probes and in the other hand the DNA input containing 8 µL of eluted cfDNA (mean ...
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bioRxiv - Physiology 2019Quote: ... Defined amounts of mRNAs at 10-fold dilutions were amplified via real-time PCR using iTaq™ Universal SYBR® Green One-Step kit (Bio-Rad, Italy) using the following RT-PCR conditions ...
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bioRxiv - Immunology 2021Quote: ... Reverse transcription followed by qPCR was performed in the same reaction using the Universal Probes One-Step PCR kit (Bio-Rad Laboratories, Hercules, CA) and the Taqman primers Cftr (Mm00445197_m1) ...
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bioRxiv - Immunology 2024Quote: ... as detailed in “RNA/DNA extraction and sequencing.” Kars RNA expression was measured in each tumor using the iTaq Universal SYBR Green One-Step Kit (Bio-Rad Cat. No. 1725150) and the Quantstudio 6 Flex System (Applied Biosystems) ...
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bioRxiv - Cell Biology 2023Quote: ... They were then washed thrice with TBST (5 minutes/wash) at room temperature and signal developed for one minute using Clarity™ Western ECL substrate (Bio-Rad, Cat #: 1705060) or Clarity Max Western ECL Substrate (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... The proteins were stained with coomassie blue R250 and PMMoV-I coat protein was quantified using a densitometer and the quantity one software (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Genomics 2022Quote: ... All PCRs described above were carried out using KOD One Master Mix (#KMM-101, TOYOBO, Osaka, Japan) with a C1000 Touch thermal cycler (Bio-Rad, Hercules, CA, USA). Primer sequences are listed in Table S3.
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bioRxiv - Immunology 2023Quote: ... The viral genome of the H7N1 virus was quantified using qRT-PCR that was conducted with the Bio-Rad iTaq™ Universal SYBR ® Green One-Step Kit (BioRad, California, USA) using primers designed for the detection of the M gene as published elsewhere 60.
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bioRxiv - Plant Biology 2019Quote: ... Bio-Beads SM-2 absorbents (152-8920, Bio-Rad) that had been washed in methanol (3 x 10 min) ...
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bioRxiv - Biophysics 2021Quote: ... 1.5 ml of BioBeads SM-2 slurry (Bio-Rad) was added to remove the detergents ...
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bioRxiv - Biophysics 2021Quote: ... After several incubation rounds with biobeads SM-2 (Biorad), the proteoliposomes were harvested the next day ...
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bioRxiv - Neuroscience 2021Quote: ... 2 million N2A cells were transfected using TransFectin (Biorad) with either CFP-ctrl. ...
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bioRxiv - Immunology 2019Quote: ... Fixed HEp-2-coated microscope slides (Kallestad®, BioRad) were blocked ...
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bioRxiv - Biochemistry 2019Quote: ... Reactions were run on an Optocon 2 thermocyler (Biorad). Primers used are listed in Table 2.
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bioRxiv - Biophysics 2021Quote: ... 37.5 μl 2% Bis-solution (161-0142, Bio-Rad), 2 μl (0.4% ...
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bioRxiv - Bioengineering 2021Quote: ... Synthetic SARS-CoV-2 RNA was purchased from BIORAD (SARS-CoV-2 Standard #COV019 and SARS-CoV-2 Negative #COV000).
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bioRxiv - Biochemistry 2020Quote: ... followed by 4 incubations with BioBeads SM-2 (BioRad) to remove the detergent ...
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bioRxiv - Cancer Biology 2020Quote: ... and supplemented with 5% 2-mercaptoethanol (Bio-Rad Laboratories) for 8 minutes at 100 °C ...
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bioRxiv - Neuroscience 2021Quote: ... treated with 2 X Laemmli sample buffer (Bio-Rad) to elute/denature protein before SDS-PAGE and immunoblotting for DAT as above ...
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bioRxiv - Cell Biology 2021Quote: ... 12.5 μl 2× ddPCR Supermix for probes (Bio-Rad), 1.25 μl R636S+SM FAM probe and primer premixture ...
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bioRxiv - Biophysics 2020Quote: ... An equal volume of Biobeads SM-2 (Bio-Rad) was added to the nanodisc mixture for detergent removal ...
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bioRxiv - Developmental Biology 2022Quote: ... Laemmli sample buffer (Bio-Rad #1610747, 5% 2-mercaptoethanol) was added to 20 µg protein and samples were boiled at 95°C for 5 minutes before being loaded onto a 10% SDS-polyacrylamide gel (Bio-Rad #4568034) ...
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bioRxiv - Molecular Biology 2022Quote: ... in a 2 mm Gene Pulser cuvette (Bio-Rad) and was subjected to electroporation at 2.3 kV ...
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bioRxiv - Molecular Biology 2021Quote: ... and 100 mM 2-Mercaptoethanol (Bio-Rad Cat#1610710), and nucleosides (Millipore Cat#ES-008-D).
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bioRxiv - Molecular Biology 2020Quote: ... 2) Chelex 100 Resin (Bio-Rad, Hercules, CA, USA); and 3 ...