Labshake search
Citations for Bio-Rad :
951 - 1000 of 9152 citations for 6 Methyl 5 4 phenyl 1 3 thiazol 2 yl 2 trifluoromethyl nicotinic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2022Quote: ... Digestion was confirmed by running 5 µL of digested or diluted samples on a 4-20% TGX Mini-Protean gradient gel (Bio-Rad), followed by staining with Gelcode Blue (Thermo Scientific ...
-
bioRxiv - Microbiology 2022Quote: ... Samples were boiled for 5 min at 95°C before separation on a gradient 4 to 20% sodium dodecyl sulfate (SDS)-polyacrylamide gel (Bio-Rad). Samples were then transferred to nitrocellulose membrane using standard methods for semidry transfer (Bio-Rad) ...
-
bioRxiv - Neuroscience 2022Quote: ... at either 95°C for 5 minutes or at 70°C for 10 minutes and resolved in a 4-15% Precast Gels (Bio-Rad, 12-well ...
-
bioRxiv - Cancer Biology 2023Quote: ... and the samples were boiled for 5 minutes before being loaded on the gel (4-15% Criterion TGX Precast Midi Protein Gel, Bio-Rad). Proteins were separated by SDS-PAGE and blotted into a polyvinylidene fluoride (PVDF ...
-
bioRxiv - Biochemistry 2023Quote: ... The samples were incubated at 95°C for 5 min and 10 μL of the sample was resolved on a 4%–15% Mini-PROTEAN® TGX Precast Gel (Biorad) and stained with Coomassie Blue.
-
bioRxiv - Biochemistry 2023Quote: ... The samples were incubated at 95°C for 5 min and 10 μL of the sample was resolved on a 4%–15% Mini-PROTEAN® TGX Precast Gel (Biorad) and stained with Coomassie blue dye.
-
bioRxiv - Plant Biology 2023Quote: ... and 10 (V/V) % beta-mercaptoethanol for 5 minutes before being loaded into a 4-20% gradient SDS-PAGE gel (Biorad: 4561096) and run according the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2024Quote: ... The membranes were then washed 4 times with PBST and then incubated in the dark for 5 min with Clarity Western ECL substrate (1705060, Bio-Rad), and imaged using the Amersham Imager 600 (GE Industries ...
-
bioRxiv - Cell Biology 2024Quote: ... boiled at 950C for 5 min and loaded into each lane of a 4–15% Mini-PROTEAN TGX Stain-Free precast gel (Bio-Rad). Colour Prestained Protein ladder was loaded in some wells in order to determine the molecular mass of proteins in the experimental samples ...
-
bioRxiv - Molecular Biology 2024Quote: ... Samples were boiled at 95°C for 5 min and proteins were separated on 4–15% Mini-Protean TGX precast protein gel (Bio-Rad) and transferred onto hydrophobic polyvinylidene fluoride (0.45 μm PVDF ...
-
bioRxiv - Immunology 2024Quote: ... were washed with 500 μl ice-cold acetone to remove TCA at 16,000g for 5 min at 4 °C and resuspended in 100 μl 1x XT sample buffer (Bio-Rad #1610791), boiled at 95 °C for 10 min ...
-
bioRxiv - Cell Biology 2024Quote: ... The samples were boiled for 5-10 minutes and then separated using SDS-PAGE with 4-12% Criterion XT Bis-Tris Gels (Bio-Rad). The proteins were transferred to PVDF membranes (Merck Millipore) ...
-
bioRxiv - Plant Biology 2024Quote: ... heat-denatured at 95°C for 5 min and separated by SDS-PAGE on home-made gels or 4-15% precast polyacrylamide gel (Bio-rad).
-
bioRxiv - Neuroscience 2024Quote: ... sample protein (5-8 µg) and molecular weight ladders were loaded onto a 4–15% Criterion TGX precast gels (Bio-Rad) for gel electrophoresis separation ...
-
bioRxiv - Neuroscience 2024Quote: ... 50 μg protein/sample was denatured at 95 °C for 5 min in Laemmli buffer with 2.5% ß-mercaptoethanol and separated with a 4–20% Mini-PROTEAN (Bio-Rad) polyacrylamide gel in 25 mM Tris ...
-
bioRxiv - Neuroscience 2024Quote: ... heated at 95 °C for 5 min and loaded on stain-free 4–15% gradient sodium dodecyl sulfate polyacrylamide gel electrophoresis gels (Bio-Rad). Separated proteins were transferred onto a Polyvinylidene difluoride (PVDF ...
-
bioRxiv - Genomics 2024Quote: ... the samples were treated with 5% 2-mercaptoethanol in Laemmli buffer and heated at 95°C for 5 minutes prior to loading into a 4-15% gradient SDS gel (Cat# 4568085, Bio-Rad) and run at 160v for 50-55 minutes ...
-
bioRxiv - Cell Biology 2024Quote: ... Samples were boiled at +90 °C for 5 min prior to protein separation using precast SDS- PAGE gradient gels (4–20% Mini-PROTEAN TGX, Bio-Rad) and transferred onto nitrocellulose membranes with the semi-dry Trans-Blot Turbo Transfer System (Bio-Rad) ...
-
bioRxiv - Molecular Biology 2022Quote: ... and incubated for 1 h with 5% blotting grade blocker (Bio-Rad). Primary antibodies were incubated overnight at 4℃ and secondary HRP-conjugated antibodies were incubated for 1 h at room temperature ...
-
bioRxiv - Microbiology 2022Quote: ... After 1 h blocking (5% non-fat milk, Bio-Rad 170–6404) at room temperature (RT) ...
-
bioRxiv - Microbiology 2024Quote: ... Diluted reaction products (1:5) were run on a 2.5% agarose (BioRad) gel in 1x TAE buffer (ThermoScientific ...
-
bioRxiv - Cell Biology 2020Quote: ... at 100V for 1 hour and blocked for 1 hour with 5% non-fat milk (Bio-Rad) before incubation at 4 C overnight with appropriate primary antibodies ...
-
bioRxiv - Immunology 2024Quote: ... blocked with 5% FBS for 1 hour and incubated with anti TGN46 antibody (#AHP500GT, BioRad, 1:2000) at 4°C overnight ...
-
bioRxiv - Biophysics 2022Quote: ... and 1 mM EDTA in 99.9% deuterium oxide using a spin desalting column (Micro Bio-Spin 6, BioRad). 15N-HSQC spectra were measured every 40 min for 106 hours at 25 °C on Bruker 600 MHz NMR spectrometer ...
-
bioRxiv - Neuroscience 2024Quote: ... The blot was washed 3 times with TBST for 5 minutes and was incubated with 10 mL of 3% BSA/TBST (w/v) with 1 µL anti-mouse-HRP secondary antibody (1:10,000; 170-6516, Bio-Rad) for 30 minutes at room temperature ...
-
bioRxiv - Cell Biology 2020Quote: ... A reaction solution of 20 Δl comprising 10 μl 2× Sso Advanced universal SYBR Green Supermix (Bio-Rad), 1 μl cDNA template (25 ng) ...
-
bioRxiv - Molecular Biology 2020Quote: ... After 2 weeks cells were stained by the sulforhodamine B and photographed by E-Gel Imager (Bio-Rad). The cloning numbers in each group were counted to calculate the inhibition rate.
-
bioRxiv - Molecular Biology 2021Quote: ... The qPCR experiments were performed according to an absolute quantification design on the Opticon 2 PCR machine (Biorad). Β2Microglobulin (B2M ...
-
bioRxiv - Bioengineering 2021Quote: ... First strand cDNA was synthesized utilizing 2 µg of total RNA and iScriptTM cDNA Synthesis Kit (Bio-Rad). A fraction (0.75 μg ...
-
bioRxiv - Bioengineering 2021Quote: ... 2 uL of bacterial culture was mixed with 10 uL 2X Sso Fast Evagreen qPCR mixture (BioRad USA). The primers were mixed into an equal forward/reverse (F/R ...
-
bioRxiv - Cancer Biology 2021Quote: ... 2 μg of total RNA was used for cDNA preparation using the iScript cDNA synthesis kit (Bio-Rad). A 1/10 dilution of cDNA was used for qRT-PCR analysis using the FastStart Universal SYBR Green Master Mix (Roche) ...
-
bioRxiv - Neuroscience 2021Quote: ... Proteins were transferred to a nitrocellulose membrane for 2 hours in a wet blot tank system (Bio-Rad), and membranes were blocked with 5% skim milk for 30 min at room temperature before incubating with primary antibody ...
-
bioRxiv - Microbiology 2021Quote: ... using primers specific for fathead minnow β-actin (Table 2) with iTaq universal SYBR green supermix (Bio-Rad).
-
bioRxiv - Molecular Biology 2020Quote: ... IPG strips were run upon SDS PAGE (12.5% acrylamide) using the Protean® II 2-D Cell (BioRad). Gels were then Coomassie blue stained (Phastgel Blue R ...
-
bioRxiv - Bioengineering 2022Quote: ... ddPCR reactions were set up as follows: 11 μL 2×ddPCR™ supermix for probes (no dUTP) (BioRad), 0.2 μL FWD primer (100 μM) ...
-
bioRxiv - Biochemistry 2022Quote: ... PCR products were confirmed by 2% agarose gel electrophoresis stained with GelRed using a 50 bp marker (BioRad). PCR products were normalized using Mag-bind Total Pure NGS ...
-
bioRxiv - Cancer Biology 2022Quote: ... 2×106 cells (extracted tissues or cell culture) were suspended in 50 μL RIPA (Radio ImmunoPrecipitaion Assay; BioRad) lysis buffer containing 200 mM PMSF (Alpha-Phenyl Methyl Sulfonyl Fluoride ...
-
bioRxiv - Genomics 2022Quote: ... These products were analyzed on a 2% agarose gel and was imaged with Gel Doc imaging system (BioRad). The observation of multiple bands implied successful targeting of the sequences by the sgRNA.
-
bioRxiv - Microbiology 2022Quote: ... Quantitative PCR was performed on 2 μl with the SsoAdvanced™ Universal SYBR® Green Supermix (Bio-Rad) and the following primer pairs ...
-
bioRxiv - Developmental Biology 2020Quote: ... Smit1/2 and TonEBP were measured by real time qPCR using SYBR Green I (Bio-Rad, Mississauga ON) and 5 μM of forward and reverse primer mix (Bio-Rad CFX384 ...
-
bioRxiv - Genetics 2021Quote: ... The enriched proteins were dissociated by the addition of 2 x Laemmli sample buffer (161-0737, Bio-Rad) and boiled at 95 °C for 10 min ...
-
bioRxiv - Biochemistry 2021Quote: ... Gels were dried for 2 h at 80°C and analysed on a Personal Molecular Imager (Bio-Rad). For RNA stability assays ...
-
bioRxiv - Molecular Biology 2021Quote: ... 10 μl of RNA were mixed with 2 μl of 50 μM oligo dT primer (Bio-Rad, 1725038) and 1 μl of 25 mM dNTP mix (NEB ...
-
bioRxiv - Immunology 2022Quote: ... 200 μL of this transformation mix was then aliquoted into pre-chilled 2 mm electroporation cuvettes (Bio-Rad) and electroporated at 1500 V with an average time constant of ∼4.5 ms using a Gene Pulser Xcell Electroporation System (Bio-Rad) ...
-
bioRxiv - Physiology 2023Quote: ... Protein samples were suspended in Laemmli Sample Buffer supplemented with 2-Mercaptoethanol (β-mercaptoethanol) (BioRad Hercules, California – USA). Then ...
-
bioRxiv - Cancer Biology 2023Quote: ... qPCR was performed using the Promega 2-step kit on a CFX96 Real-Time PCR System (Bio-Rad) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... heated to 50°C for 2 min before addition of 40 µl molten CleanCut agarose (Bio-Rad 1703594), vortexing vigorously for 5 s before pipetting in plug mould (Bio-Rad 1703713 ...
-
bioRxiv - Plant Biology 2023Quote: ... 2 ug RNA was used to synthesize cDNA with the iScript™ cDNA Synthesis Kit (Bio-Rad 1708890). The SsoAdvanced™ Universal SYBR® Green Supermix (Bio-Rad 1725271 ...
-
bioRxiv - Physiology 2023Quote: ... and samples of four larvae each were homogenized in 100 μl 2× SDS sample buffer (Bio-Rad #1610737) containing 5% (355 mM ...
-
bioRxiv - Cell Biology 2023Quote: ... Three hundred micrograms of protein was precipitated using a ReadyPrep 2-D Cleanup Kit (Bio-Rad Laboratories, USA) at −20 °C overnight ...