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Citations for Bio-Rad :
51 - 100 of 1639 citations for Ubiquitin Conjugating Enzyme E2 K UBE2K Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... and rabbit F(ab’)2 anti-rat IgG conjugated to FITC (STAR17B, BioRad).
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bioRxiv - Cancer Biology 2022Quote: ... the cells were stained with Annexin-V-FITC and PI (Bio-Rad, ANNEX20F) following FACS analysis using Gallios flow cytometer (Beckman Coulter ...
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bioRxiv - Microbiology 2023Quote: ... GM testing with the Platelia enzyme-linked immunosorbent assay (ELISA) (Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA extracted from tail tips using proteinase K digestion and Chelex 100 Resin (Biorad 1432832) was amplified for 30 cycles at 95 °C ...
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bioRxiv - Bioengineering 2020Quote: ... Detection of the test articles was performed using a rabbit anti-FITC IgG (Biorad) followed by anti-rabbit IgG Alexa FluorTM 488 (Invitrogen) ...
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bioRxiv - Biochemistry 2020Quote: ... Enzyme concentrations were determined using a Bio-Rad Protein Assay Kit (Bio-Rad). Mutations in Hs ProXp-ala and Cc ProXp-Ala were introduced using either QuikChange Site-Directed Mutagenesis (Agilent ...
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bioRxiv - Microbiology 2022Quote: ... The gel was exposed to a phosphor screen (Molecular Imager FX Imaging screen-K, Bio-Rad) for 24 hr and imaged using the Amersham Typhoon 5.
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bioRxiv - Plant Biology 2020Quote: The protein content in the enzyme extracts was determined by using Bradford assay (BioRad) (Bradford ...
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bioRxiv - Biochemistry 2020Quote: ... The detergent/lipid/enzyme solution was mixed with SM-2 bio-beads (Bio-Rad) for 2 h at 4 0C to remove the detergent ...
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bioRxiv - Molecular Biology 2021Quote: ... a storage phosphor screen (K-Screen; Kodak) and Molecular Imager FX in combination with QuantityOne software (BioRad) were used.
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bioRxiv - Microbiology 2023Quote: ... Samples were then stained for the intracellular expression of FITC-anti-CD3 (CD3-12; MCA1477F BioRad), R718-anti-Ki-67 (B56 ...
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bioRxiv - Synthetic Biology 2020Quote: ... 0.5×106 cells per plug: plugs have been treated with Proteinase K (CHEF genomic DNA plug kit, Biorad) and restriction-digested by BamHI (NEB ...
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bioRxiv - Biophysics 2020Quote: ... Oligo duplexes were ligated in equimolar concentrations using T4 enzyme (Bio-rad, Hercules, CA, USA) for 2 h ...
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bioRxiv - Molecular Biology 2020Quote: ... 10 min for enzyme deactivation in a thermal cycler (T100, Bio-Rad, Pleasanton, CA, USA) with the temperature ramp rate of 2 °C/s ...
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bioRxiv - Microbiology 2023Quote: Values of the enzyme assay curves were obtained with CFX Maestro (Bio-Rad Laboratories, Inc.), and were illustrated and analyzed using GraphPad Prism (GraphPad Software ...
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bioRxiv - Bioengineering 2023Quote: ... For cDNA synthesis, an AccuPower RocketScript Cycle RT PreMix (K-2202, Bioneer) and a T100 Thermal Cycler (Bio-Rad) were used ...
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bioRxiv - Neuroscience 2022Quote: ... GFP+ cells were captured into single tube with 100 μl lysis buffer containing Proteinase K and DNase (Bio-Rad). The sorted cells were then incubated for 10 min ...
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bioRxiv - Genetics 2024Quote: ... Storage phosphor screens (K-Screen, Kodak) and Molecular Imager FX were used to quantify spot intensities with QuantityOne (BioRad).
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bioRxiv - Evolutionary Biology 2020Quote: ... buffer and enzyme were pipetted into the Hard-Shell PCR 96-well plates (Bio-Rad, USA) and then covered by Microseal ‘B’ seal (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... Purification steps were followed by SDS-PAGE and purified enzymes quantified by Bradford method (Bio-Rad).
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bioRxiv - Cancer Biology 2024Quote: ... and 70°C to denature the enzyme using DNA Engine Dyad thermal cycler (Bio-Rad Laboratories). The synthesized cDNA was stored at -20°C for further use.
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bioRxiv - Immunology 2022Quote: ... samples were stained with 100 μl permeabilization buffer containing α-CD3e-FITC (CD3-12, Bio-Rad, Hercules, CA) for 30 minutes at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... The concentrated eluate was buffer-exchanged into 20 mM K-phosphate (pH 7.0) using a pre-washed PD10 size-exclusion column (Bio-Rad) using gravity flow ...
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bioRxiv - Systems Biology 2020Quote: ... The concentration of proteins was measured using an enzyme-linked immunosorbent assay reader (BioRad, Hercules, CA, USA) at 570 nm using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... We used FITC-conjugated mouse anti-pig CD4 alpha (clone MIL17, Bio-Rad AbD Serotec, Puchheim, Germany, 1:20) and Alexa Fluor 647-conjugated mouse anti-pig CD8α (clone 76-2-11 ...
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bioRxiv - Plant Biology 2022Quote: ... The chemiluminescent signals were developed by WesternBright ECL kit (LPS Cat# K-12045-D20) and documented on the ChemiDoc MP system (Bio-rad).
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bioRxiv - Plant Biology 2022Quote: ... WesternBright ECL kit was used to detect the chemiluminescent signals (LPS Cat# K-12045-D20) and ChemiDoc MP system (Bio-rad) was used to capture the image.
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bioRxiv - Plant Biology 2021Quote: ... membrane was incubated 1 min in 1 ml enzyme solution (Clarity™ Western ECL, BIORAD, Les-Ulis, France) and 1 ml Luminol/enhancer solution (Clarity™ Western ECL ...
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bioRxiv - Immunology 2023Quote: ... and 5 h later cells were stained with mAbs against CD4-PerCP-Cy5.5 (as in Table S2) and CD8β-FITC (clone PPT23, Bio-Rad). The cells were fixed and permeabilized with BD Cytofix/Cytoperm (BD Biosciences ...
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bioRxiv - Molecular Biology 2021Quote: ... purified DNA was quantified by qPCR using SYBR Green (Bioneer; K-6251) and the CFX Connect Real-Time PCR system (Bio-Rad; 1855201). We confirmed a subset of strongly bound cohesin sites overlapping with the binding sites of condensin (Dowen et al ...
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bioRxiv - Cell Biology 2020Quote: ... after which the plates were air-dried for 30 min and developed using a K-screen and FX-Imager (Bio-Rad Laboratories).
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bioRxiv - Biochemistry 2024Quote: ... The enzyme was purified using a 5-mL Ni-charged EconoFit Nuvia IMAC Column (Bio-Rad, Hercules, CA, USA) and a linear gradient of 10 - 250 mM imidazole in binding buffer (100 mL in total at 2.5 mL/min flow rate) ...
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bioRxiv - Molecular Biology 2021Quote: ... pH 9.0 running buffer and visualized using the FITC channel set at 100 µM resolution in low intensity mode on a GelDoc Pharos imager (BioRad Laboratories). ImageQuant software was used to analyse the fraction of probe bound in each lane and GraphPad Prism version 8 software was used to calculate the Kd ...
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bioRxiv - Cell Biology 2023Quote: ... and cell death was performed using Annexin V-FITC/PI apoptosis detection kit following the protocol described by the manufacturer (Bio-Rad).
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bioRxiv - Biochemistry 2020Quote: ... the cleaved His6 tag was removed and the enzymes were buffer exchanged into 200 mM ammonium acetate (pH 7.5) using Micro Bio-Spin 6 columns (Bio-Rad). His6-affinity tagged HalA2 was likewise digested with thrombin to remove the His6 tag ...
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bioRxiv - Microbiology 2020Quote: ... 10 U of HindIII restriction enzyme and 1.1 μL of a 20X primers-probes mix (ddPCR mutant assay) provided by Bio-Rad (concentrations and sequences of probes are not provided by the manufacturer but they guaranty the validation by Minimum Information for Publication of Quantitative Real-Time PCR Experiments [MIQE] guidelines) ...
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bioRxiv - Cell Biology 2021Quote: Purified substrates and enzymes were centrifuged (16,000 g, 5 min, 4°C) before protein concentration was determined by Bradford assay (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... the integrity of bacmid DNA was analyzed by digestion with restriction enzyme XhoI and separation in 0.8% PFGE agarose (Bio-Rad) gels and 0.5×TBE buffer by pulsed-field gel electrophoresis at 6 V/cm ...
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bioRxiv - Microbiology 2024Quote: ... 20 μg of plasmid DNA was linearized by restriction enzyme treatment (pXS2 and pXS6 plasmids: MluI; pZJM plasmids: NotI) and electroporated in 4 mm cuvettes (BioRad GenePulser Xcell ...
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bioRxiv - Microbiology 2024Quote: ... the integrity of bacmid DNA was analysed by digestion with restriction enzyme XhoI and separation in 0.8% PFGE agarose (Bio-Rad) gels and 0.5×TBE buffer by pulsed-field gel electrophoresis at 6 V/cm ...
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bioRxiv - Molecular Biology 2021Quote: ... the supernatant was mixed with 5× RT Reaction Buffer and 10× RT Enzyme Mix and transferred to a thermal cycler (Bio-Rad). Cycling conditions were as follows ...
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Heterologous expression of Dehalobacter spp. respiratory reductive dehalogenases in Escherichia colibioRxiv - Microbiology 2021Quote: ... the purity of the final enriched enzyme was estimated by band density using Image Lab v6.1 Software (Bio-Rad Laboratories, Inc.); the SDS-PAGE of TmrA and lane density for purity analysis is shown in Figure S2 and protein yield is in Table S6 of SI Text 3.
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bioRxiv - Microbiology 2021Quote: ... Then the samples were treated at 60oC for 1 h and then enzyme deactivated 95oC for 15 min in a thermocycler (Bio-rad). After the treatment ...
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bioRxiv - Microbiology 2020Quote: ... Washed cells were embedded in 2 % Low melt agarose plugs and cells were lysed (1 hr at 37°C) with lysozyme enzyme (100 μl) using CHEF Bacterial Genomic DNA Plug Kit (Bio-Rad). DNA containing plugs were washed twice with nuclease free water then treated with Proteinase K (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... the integrity of bacmid DNA was analyzed by digestion with restriction enzyme XhoI and separation in 1% PFGE agarose (Bio-Rad) gels and 0.5×TBE buffer by pulsed-field gel electrophoresis at 6 V/cm ...
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bioRxiv - Biochemistry 2022Quote: ... Enzyme purity and molecular weight were estimated by 12 % SDS-PAGE using mini-PROTEAN 3 system (BioRad, Hercules, California, United States). Gels were stained using PageBlue Protein Staining Solution (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... Immunofluorescence labelling was performed to analyze the DC markers using FITC labeled mouse-anti-chicken MHC-II in combination with mouse anti-chicken CD40 (Bio-Rad, Hercules, CA), mouse anti-chicken CD11c (8F2 ...
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bioRxiv - Cell Biology 2021Quote: ... coli antibodies (1:100, Bio-Rad Antibodies, 4329-4906), followed by DyLight 650-conjugated donkey anti-rabbit IgG antibodies (1:1000 ...
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bioRxiv - Developmental Biology 2020Quote: Levels of PAI1 in human plasma were measured using a Bio-Plex multiplex enzyme immunoassay (Customized Human Cancer Biomarker Assay, Bio-Rad Laboratories). Plasma was diluted 1:4 in assay diluent prior to performing the assay ...
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bioRxiv - Neuroscience 2021Quote: ... and the Clarity Western enhanced chemiluminescence (ECL) system served as the substrate for the peroxidase enzyme (Cat# 1705060, Bio-Rad, Hercules, CA). A list of antibodies and reagents used for the dot blot experiments ...