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Citations for Bio-Rad :
51 - 100 of 231 citations for SARS Coronavirus Spike Glycoprotein S1 Mosaic N Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... coli Dh5α using IProof DNA polymerase from BioRad and restriction enzymes from New English Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... coli Gene Pulser® (Biorad, Hercules, CA, USA), as recommended by the supplier ...
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bioRxiv - Biophysics 2023Quote: ... coli was purchased from Bio-Rad (Hercules, CA) and received as a lyophilized powder ...
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bioRxiv - Cancer Biology 2022Quote: ... qPCR was performed using human-specific primers (Table S1) and iQ SYBR Green Supermix (Bio-Rad), with annealing temperature optimized by gradient PCR ...
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bioRxiv - Biochemistry 2022Quote: ... stock solution of 40% neutral acrylamide and N,N’-methylenebisacrylamide in a 19:1 ratio (Bio-Rad) were mixed with a stock solution of 40% positively charged (3-acrylamidopropyl)-trimethylammonium chloride (75% wt ...
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bioRxiv - Biophysics 2021Quote: ... coli lysate was purchased from Bio-Rad (Hercules, CA) and received as a lyophilized powder ...
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bioRxiv - Microbiology 2021Quote: ... coli SM10 λpir by electroporation (MicroPulser Electroporator, Bio-Rad). The parental E ...
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bioRxiv - Cell Biology 2021Quote: ... coli antibodies (1:100, Bio-Rad Antibodies, 4329-4906), followed by DyLight 650-conjugated donkey anti-rabbit IgG antibodies (1:1000 ...
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Multimodal tubulin binding by the yeast kinesin-8, Kip3, underlies its motility and depolymerizationbioRxiv - Biophysics 2021Quote: ... The protein eluates were adjusted to 250 mM NaCl and loaded onto a Uno S1 (Bio-rad) ion exchange column ...
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bioRxiv - Cell Biology 2021Quote: ... Target gene cDNA was amplified using primers (Table S1) and Sso Advanced Universal SYBR Green Supermix (BioRad) on a CFX384 Touch Real-Time PCR Detection system (BioRad) ...
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bioRxiv - Molecular Biology 2020Quote: ... qRT PCR was performed with gene-specific primers (Table S1) using iTaq universal SYBR Green Supermix (BioRad) for 45 cycles on CFX96 Real time system (BioRad) ...
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bioRxiv - Developmental Biology 2021Quote: ... 4 hpf (n=40) or 48 hpf (n=25) embryos using the Aurum Total RNA Mini Kit (Bio-Rad). cDNA was then prepared with the RevertAid reverse transcriptase (Thermo Fisher) ...
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bioRxiv - Neuroscience 2020Quote: ... The post-nuclear S1 fraction (supernatant) was collected and protein concentration measured using the BCA protein assay (Biorad).
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bioRxiv - Synthetic Biology 2022Quote: ... coli recipient cells via electroporation with a MicroPulser (Bio-Rad).
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bioRxiv - Cell Biology 2023Quote: ... coli antibodies (Bio-Rad, Bio-Rad, 4329-4096, 1:100), and goat anti-Rabbit HRP (ThermoFisher ...
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bioRxiv - Cell Biology 2023Quote: ... coli antibodies (Bio-Rad, Bio-Rad, 4329-4096, 1:100), and goat anti-Rabbit HRP (ThermoFisher ...
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bioRxiv - Microbiology 2024Quote: ... coli TOP10 cells with the Gene Pulser electroporation system (BioRad) set at 200 Ω ...
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bioRxiv - Biophysics 2023Quote: ... extracted agarose gel parts were divided by cutting several times and purified via Freeze N′ Squeeze columns (Freeze N′ Squeeze, 7326165, BioRad) according to the manufacturer’s instructions using a benchtop centrifuge (Biofuge fresco ...
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bioRxiv - Biophysics 2019Quote: ... N-methylene-bis-acrylamide (Bio-Rad, Herculers, USA) are added ...
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bioRxiv - Developmental Biology 2021Quote: ... qPCR analysis was performed using the primers in Table S1 on CFX384 Touch Real-Time PCR Detection System (BioRad). Data were analysed using the CFX Maestro Software (BioRad) ...
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bioRxiv - Microbiology 2021Quote: ... S1 nuclease digestion analyzed with PFGE was performed using a CHEF Mapper XA apparatus (Bio-Rad Laboratories, Hercules, CA). Briefly ...
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bioRxiv - Biophysics 2022Quote: ... 2 μL of cDNA was mixed with 2 μL of forward and reverse primers (2.5 μM each, Table S1,) and 1x SYBR Green Supermix (BioRad) for a final reaction volume of 20 μL ...
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bioRxiv - Plant Biology 2023Quote: ... with 7.5 ng cDNA and 200 nM primers (Table S1) on a CFX Opus 384 Real-time PCR System (BioRad). Mean PCR reaction efficiencies were calculated for each primer pair with LinRegPCR (Ruijter et al. ...
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bioRxiv - Genetics 2023Quote: ... For pulse-field gelectrophoresis of the S1- treated samples a CHEF mapper XA Pulse Field Electrophoresis System (Bio-Rad Laboratories Inc. ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 specific genome levels were measured by qPCR using SsoFast EvaGreen supermix (Bio-Rad) according to manufacturer’s protocol.
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bioRxiv - Microbiology 2022Quote: ... The relative abundance of Spike transcripts was quantified by quantitative real-time PCR (qPCR) using CFX384 machine (BioRad) with a fluorescence reporter (Thermo Fisher ...
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bioRxiv - Microbiology 2020Quote: ... coli DH10B (53) with a MicroPulser electroporator (Bio-Rad -Hercules, USA). The resulting libraries were analyzed for the percentage of plasmids carrying genomic DNA and the average size of the insert they contained.
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bioRxiv - Bioengineering 2022Quote: ... 0.2 μM each of the forward and reverse primers (Supplementary Table S1) and iTaq Universal SYBR Green Supermix (Bio-Rad). All biological replicates were measured in technical duplicates.
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bioRxiv - Plant Biology 2021Quote: ... 10 µl PCR reactions were performed in technical duplicate with SensiFAST SYBR No-ROX (Meridian Bioscience) with 4 ng cDNA and 300 nM primers (Table S1) on a CFX96 Real-time PCR System (BioRad). Mean PCR reaction efficiencies were calculated for each primer pair with LinRegPCR (Ruijter et al. ...
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bioRxiv - Microbiology 2020Quote: ... Real-time PCR was performed using SYBR Green (Applied Biosystem – 4309155) and primers listed in Table S1 at an annealing temperature of 60°C on a CFX96 thermal cycler (Biorad). Generated data were analysed using the CFX Manager Software (Biorad).
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bioRxiv - Bioengineering 2019Quote: ... and the selected primers (Table S1, Supplementary Information) to perform qPCR using CFX Connect™ Real-Time System (Bio-Rad) under the following conditions ...
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bioRxiv - Cell Biology 2021Quote: ... Primary and secondary antibodies are listed in Table S1 and were detected using appropriate fluorescent channels of chemiluminescent channels of a Chemidoc MP (BioRad), using Clarity Max Western ECL Substrate (BioRad).
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bioRxiv - Cell Biology 2023Quote: ... Quantitative PCRs were performed with specific oligonucleotide pairs as indicated in Supplemental Table S1 using the iTaq Universal SYBR Green Supermix (BioRad) and a Rotor-Gene 6000 instrument (Corbett) ...
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bioRxiv - Bioengineering 2019Quote: ... by means of O/N wet transfer (Bio-Rad) 4 °C at 30 V ...
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bioRxiv - Neuroscience 2023Quote: ... SARS-CoV-2 RT-qPCR reactions (10 µL) using the iTaq Universal Probe One-Step Kit (BioRad) and SARS-CoV-2 N1 primers/probe mix (Stock# 10006713 ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 μl of sequin spike (https://www.sequinstandards.com) was added to 14 μl of RNA for cDNA synthesis with SsoAdvanced iScript (Bio-Rad), followed by RT-qPCR using SsoAdvanced master mix (Bio-Rad) ...
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bioRxiv - Microbiology 2022Quote: ... Lysates for quantitative Spike western blotting were loaded onto TGX Stain-Free 4-20% gradient gels (Bio-Rad, 4568094) (Fig ...
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bioRxiv - Microbiology 2022Quote: ... coli cells were transformed using the Pulser XCell system (Bio-Rad, USA), with the following conditions ...
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bioRxiv - Molecular Biology 2021Quote: ... and 6m (Table S1) were constructed through a two-step PCR (Fig. 2A) using a S1000™ thermal cycler (Bio-Rad). The first PCR amplification was performed in 20 µL reaction mixture ...
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bioRxiv - Molecular Biology 2022Quote: ... ∼500bp fragments of sRNA factors were amplified from cDNA of ISE6 cells using primers listed in Table S1 using iProof High- Fidelity Master Mix (Bio-Rad) and the amplicons were treated with XhoI to insert them in the XhoI site of pLitmus28i (NEB) ...
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bioRxiv - Plant Biology 2022Quote: Expression analysis on a set of different genes involved in UPR/ERQC/ERAD/PCD (see Supplementary Table S1) was performed with the SYBR Green Supermix (Bio-Rad) on StepOnePlus Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Plant Biology 2019Quote: ... introns and exons as well as a large part of promoters were amplified using appropriate primers (Table S1) and the iProof enzyme (Bio-Rad), digested with appropriate restriction enzymes and fused in frame with HA or YFP tags in home-made binary vectors derived from pGREEN0229 ...
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bioRxiv - Immunology 2020Quote: ... with 18S (Hs99999901_s1) as housekeeping control were carried out on RNA reverse transcribed to cDNA with an iScript cDNA synthesis kit (Bio-Rad), using Taqman chemistry and gene-specific probes purchased from Applied Biosystems ...
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bioRxiv - Plant Biology 2022Quote: ... the genomic region spanning the gRNA target site was PCR-amplified (primers are listed in Table S1) from WT and gene-edited tree genomic DNA using iProof GC master mix (Bio-Rad) and sequenced ...
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bioRxiv - Cell Biology 2023Quote: Samples were loaded onto e-PAGEL gel (ATTO) or TGX FastCast gel (Bio-Rad), electrophoresed ...
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bioRxiv - Microbiology 2022Quote: ... anti-Metapneumovirus N mouse monoclonal (1:25) (MCA4674, Bio-Rad), or anti-Influenza A nucleoprotein (NP ...
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bioRxiv - Immunology 2021Quote: ... Luminex beads were coupled to the Spike protein as previously described (63) and added to a Bio-Plex plate (BioRad). Beads were washed with PBS 0.05% tween using a magnetic plate washer (MAG2x program ...
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bioRxiv - Cancer Biology 2023Quote: ... KM12 or HCT116 cells (10 million) along with the spike-in control (20%) were carefully resuspended in cell suspension buffer (BioRad). The resultant homogeneous single-cell suspensions were embedded in agarose and transferred into plug molds (Bio-Rad CHEF Mammalian Genomic DNA plug kit) ...
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bioRxiv - Plant Biology 2019Quote: ... see Table S1 for primer sequences) and was performed using a CFX Connect Real-Time PCR Detection System (Bio-Rad, Watford, UK) with 40 cycles of 95°C-10s ...
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bioRxiv - Immunology 2022Quote: The levels of 48 cytokines were measured in the PF fluid using a multiplex suspension bead immunoassay (BioRad, CA, USA; Table S1) as previously described 20 ...