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Citations for Bio-Rad :
51 - 100 of 163 citations for Recombinant E. coli LT IIa His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... Imaging was done using Hi-sensitivity chemiluminescence settings under ChemiDoc XRS+ (BioRad).
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bioRxiv - Microbiology 2020Quote: ... T7-tagged templates were generated with the iProof High-Fidelity PCR Kit (Bio-Rad) using sequences cloned into plasmids pIB (LacZ) ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli recipient cells via electroporation with a MicroPulser (Bio-Rad).
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bioRxiv - Cell Biology 2023Quote: ... coli antibodies (Bio-Rad, Bio-Rad, 4329-4096, 1:100), and goat anti-Rabbit HRP (ThermoFisher ...
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bioRxiv - Cell Biology 2023Quote: ... coli antibodies (Bio-Rad, Bio-Rad, 4329-4096, 1:100), and goat anti-Rabbit HRP (ThermoFisher ...
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bioRxiv - Microbiology 2024Quote: ... coli TOP10 cells with the Gene Pulser electroporation system (BioRad) set at 200 Ω ...
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bioRxiv - Microbiology 2020Quote: ... coli DH10B (53) with a MicroPulser electroporator (Bio-Rad -Hercules, USA). The resulting libraries were analyzed for the percentage of plasmids carrying genomic DNA and the average size of the insert they contained.
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bioRxiv - Biochemistry 2021Quote: ... Recombinant XRCC4-6xHis was immobilised on a GLC chip (BioRad Laboratories) in the vertical orientation ...
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bioRxiv - Immunology 2019Quote: Lung homogenates and a pre-stained recombinant protein ladder (Bio-Rad) were run in a 12% SDS-polyacrylamide gel and were transferred to a nitrocellulose membrane (Bio-Rad) ...
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bioRxiv - Immunology 2023Quote: ... Serially diluted recombinant human IgG1 or human IgG4 (BioRad, Hercules, CA) with unrelated specificities were used for quantification in separate wells on the same plate.
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bioRxiv - Neuroscience 2023Quote: ... the His-FUS proteins were mixed with Profinity IMAC Ni2+-charged resin (Bio-Rad) for 30 min at 20 °C ...
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bioRxiv - Biophysics 2022Quote: ... His6-tagged proteins were purified using an Ni-NTA affinity column (Profinity IMAC Ni-Charged, BioRad).
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bioRxiv - Microbiology 2022Quote: ... coli cells were transformed using the Pulser XCell system (Bio-Rad, USA), with the following conditions ...
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bioRxiv - Microbiology 2019Quote: ... were coated with 1 μg/ml recombinant YFV NS1 (Biorad, cat # PIP052A) in 50 mM carbonate buffer (pH ...
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bioRxiv - Neuroscience 2021Quote: ... using known concentrations of recombinant mouse IFN-γ (Bio-Rad, Oxford, UK) and a capturing of anti- IFN-γ mAb ...
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bioRxiv - Immunology 2020Quote: ... These recombinant proteins were incubated with Affi-Prep Polymyxin Media (Bio-Rad) for 4 h at 4 °C to remove endotoxins and endotoxin-bound proteins ...
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bioRxiv - Physiology 2023Quote: Human recombinant monoclonal antibodies to mouse ERFE were produced by Bio-Rad using HuCAL technology ...
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bioRxiv - Physiology 2023Quote: Human recombinant monoclonal antibodies to mouse ERFE were produced by Bio-rad using the HuCAL technology ...
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bioRxiv - Plant Biology 2023Quote: ... and then evaluated by immune-decoration analysis using a monoclonal His-antibody (AbHis, Bio-rad). Pichia transformants expressing FHS-CELLOX was already available (Scortica et al. ...
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bioRxiv - Immunology 2024Quote: ... The membrane was then incubated with mouse anti-His antibody (catalog number #6200203, Bio-Rad), diluted 1:1,000 in the blocking solution and incubated overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: Samples were loaded onto e-PAGEL gel (ATTO) or TGX FastCast gel (Bio-Rad), electrophoresed ...
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bioRxiv - Bioengineering 2020Quote: ... 0.4% β-mercaptoethanol) per lane or 1 μL of strep-tagged unstained protein standards (Bio-Rad # 1610363) and run at 100 V for 2 h in Tris-HCl-Glycine-SDS running buffer ...
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bioRxiv - Microbiology 2023Quote: ... coli DH5α using a Bio-Rad Gene Pulser II Electroporation system (Bio-Rad) with the following parameters ...
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bioRxiv - Biochemistry 2021Quote: ... Immunoblots were visualized using horseradish peroxidase–tagged secondary antibody and chemiluminescence (Clarity Western ECL Substrate; BioRad, Hercules, CA). The antibodies used included those directed to lamin A/C ...
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bioRxiv - Neuroscience 2021Quote: ... His6-tagged proteins were then captured from the lysate by Ni2+-IDA chromatography (Profinity IMAC resin, Bio-Rad) under gravity flow and washed with 35mM Imidazole ...
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bioRxiv - Cell Biology 2022Quote: Purified γ-TuRC preparations (GCP3- and GCP6-tagged) were resuspended in 20 μL of Laemmli Sample buffer (Biorad) and were loaded on a 4%–12% gradient Criterion XT Bis-Tris precast gel (Biorad) ...
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bioRxiv - Systems Biology 2021Quote: ... Quantitative analyses of blotted hCDKL5 and His-Neuropinilin were carried out with Image Lab software (BioRad) and the volumetric yield was derived considering the final biomass concentration (OD600 ...
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bioRxiv - Microbiology 2020Quote: ... The plates were washed and incubated with mouse anti-His (Bio-Rad MCA-1396; 1:1000) followed by horseradish peroxidase (HRP)-conjugated goat anti-mouse secondary antibody (MerckMillipore AP124P ...
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bioRxiv - Microbiology 2023Quote: ... Binding of VHHs was detected using a mouse anti-HIS-tag antibody (Biorad, MCA1396, 1/2000) and an AF647 conjugated donkey anti mouse IgG antibody (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... coli strain S17-1 using a Gene Pulser Xcell™ (Bio-Rad, CA, USA) with the conditions of 2.5kV ...
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bioRxiv - Microbiology 2022Quote: ... coli CA434 cells by electroporation using a Gene Pulser Xcell Electroporation System (Bio-Rad Laboratories Ltd ...
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bioRxiv - Microbiology 2021Quote: Recombinant proteins (10 μg) were applied to 4-20% gradient polyacrylamide gels (Bio-Rad) after heating them for 20 minutes at 95°C in 2x Laemmli buffer with 2% β-mercaptoethanol (BME) ...
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bioRxiv - Cancer Biology 2023Quote: ... fresh 1ng/ml recombinant human fibroblast growth factor (FGF) basic (BioRad, Veenendaal, The Netherlands) and 0.1mM L-ascorbic acid (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... fresh 1ng/ml recombinant human fibroblast growth factor (FGF) basic (BioRad, Veenendaal, The Netherlands) and 0.1mM L-ascorbic acid (Sigma Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... Final recombinant protein concentrations were determined using Quick Start Bradford Protein Assays (Bio-Rad) with bovine serum albumin as the reference protein for standard curves ...
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bioRxiv - Cell Biology 2023Quote: ... The recombinant protein was purified using the Ni-NTA (Biorad IMAC Ni-Charged Resins) affinity chromatography under denaturing conditions ...
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bioRxiv - Developmental Biology 2024Quote: The concentration of recombinant proteins was determined through a Bradford Assay (Bio-Rad, #5000006) using a NanoDrop One Spectrophotometer (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... and then incubated for at least 16 hours at 4°C with either mouse monoclonal anti-His (1:2,000; AD1.1.10, Biorad) or mouse anti-RNA pol (1:5,000 ...
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bioRxiv - Biochemistry 2022Quote: ... and the protein was passed through Hi-Q anion exchange column (Bio-Rad Laboratories, Hercules, CA, USA) to remove minor nucleic acid contamination ...
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bioRxiv - Plant Biology 2021Quote: Purified His and GST fusion proteins or GST alone (500 ng) were blotted onto nitrocellulose membrane (BioRad). The nitrocellulose membrane was rinsed with TBS-T buffer (10 mM Tris-HCl at pH 7.4 ...
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bioRxiv - Microbiology 2020Quote: ... Eluents were collected and incubated with 50μL of packed Ni-NTA resin (Bio-Rad His-Pur 88222) at room temperature with nutating for 30 min ...
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bioRxiv - Molecular Biology 2022Quote: ... and cultures were induced with IPTG and His-MBP-ORC2 was purified on Ni-NTA beads (BioRad). Purified protein was injected into rabbits for serum generation and collection (Cocalico Biologicals) ...
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bioRxiv - Biochemistry 2022Quote: ... blots were imaged using a Protein Simple FluorChem E with Clarity Western ECL Substrate (Bio-Rad). Band intensity was measured using ImageJ (NIH).
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bioRxiv - Biochemistry 2022Quote: ... Protein was detected using a Protein Simple FluorChem E with Clarity Western ECL Substrate (Bio-Rad). Band intensity was measured using ImageJ (NIH).
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bioRxiv - Microbiology 2020Quote: ... coli isolates was performed by macrorestriction with XbaI followed by PFGE (Chef DRII, Bio-Rad), according to the PulseNet protocol with pulses oscillating from 2.16 to 63.8 s for 21.5 hours (Ribot et al. ...
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bioRxiv - Biochemistry 2021Quote: ... coli cells and purified using immobilized metal affinity chromatography and a Profinia Purification System (BioRad) and size exclusion chromatography via an ÄKTA fast protein liquid chromatography system (GE Healthcare) ...
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bioRxiv - Microbiology 2022Quote: ... His6 tagged proteins were detected with anti-His6 primary antibody (Pierce; 1:6,000) and antimouse secondary antibody (Biorad; 1:10,000). GroEL was used as cytoplasmic control and detected as above ...
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bioRxiv - Plant Biology 2024Quote: ... in 0.5 % milk in TBS-Tween 0.1 % to reveal HA-tagged NIN proteins following chemiluminescence revelation using the Clarity Western ECL Substrate (Bio-Rad) and the ChemiDoc Touch imaging system (Bio-Rad).
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bioRxiv - Biochemistry 2024Quote: ... the membranes were incubated with fluorescent tagged secondary antibodies for 45 minutes at RT followed by imaging on a fluorescence scanner imaging system (Biorad).
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bioRxiv - Microbiology 2020Quote: ... was used to purify the C-terminal 6X-His EfgA with 1 mL Ni-NTA columns (Bio-Rad). Columns were equilibrated with 10 mL of Buffer A at 1 mL/min prior to loading lysates ...