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Citations for Bio-Rad :
51 - 94 of 94 citations for QT Nickel Plating Process Agent For Removing Copper Impurity since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were then transferred to a nitrocellulose membrane (GE Water and Process Technologies) using a wet transfer protocol and a Mini Trans-Blot Cell (Bio-Rad) transfer apparatus at 4C ...
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bioRxiv - Biophysics 2022Quote: The purified proteins of V2HeR3 were reconstituted into a mixture of POPE and POPG membranes (molar ratio = 3:1) with a protein-to-lipid molar ratio of 1:20 by removing DDM using Bio-Beads (SM-2; Bio-Rad, CA, USA). The reconstituted samples were washed three times with 1 mM NaCl and 2 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Biochemistry 2020Quote: ... and the supernatant then batch bound overnight at 4 °C to nickel-charged IMAC resin (Profinity, BioRad) which had been equilibrated with LC Lysis Buffer ...
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bioRxiv - Cell Biology 2023Quote: ... intermediate and soluble pooled fractions were mixed with Laemmli sample buffer without reducing agent (BioRad). After boiling 5 min at 95°C ...
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bioRxiv - Cell Biology 2023Quote: ... The protein concentration was determined applying the copper-based DC Protein Assay (Bio-Rad, Munich, Germany) according to the manufactureŕs protocol ...
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bioRxiv - Neuroscience 2023Quote: ... The protein concentration was monitored periodically during the process using Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad, Hercules, California, USA) and concentrated to approximately 100-200 μM.
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bioRxiv - Microbiology 2022Quote: ... and analysed according to ISO 11290-1 (63) with selective enrichment in half-Fraser and Fraser broth (Oxoid) and final plating on RAPID’L.mono agar (Bio-Rad).
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bioRxiv - Cell Biology 2024Quote: ... we established co-cultures by plating specific numbers of different cell types measured by a cell counter (TC10, BioRad). After 3 hours ...
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bioRxiv - Microbiology 2024Quote: ... Supernatants of cell lysates were passed through a nickel bead column (Profinity IMAC Ni-Charged Resin, Bio-Rad) to allow binding of lm_ApbE-6xHis ...
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bioRxiv - Microbiology 2023Quote: ... His-tagged proteins in the cleared lysate were purified using Profinity™ IMAC Nickel Charged Resin (Bio-Rad) and eluted in 50 mM sodium phosphate buffer with 300 mM NaCl pH 8 and 300 mM imidazole (Fischer Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... and detected by the addition of chemiluminescent agent ClarityTM Western ECL Substrate (Cat# 1705060, Bio-rad).
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bioRxiv - Biochemistry 2020Quote: ... and the supernatant was mixed with urea (0.5 M final concentration) before batch binding overnight at room temperature (RT) to nickel-charged IMAC resin (Profinity, BioRad) that had been previously equilibrated with the DARET Lysis Buffer ...
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bioRxiv - Microbiology 2020Quote: ... Reducing agent was added and samples were run on a 4 - 15% SDS gradient gel (Bio-Rad) at 60 V for 40 min followed by 110 V for 45 min ...
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bioRxiv - Neuroscience 2024Quote: ... was used for double-stranded DNA intercalating agents and SsoFastTM Probes Supermix (172-5231, BioRad, Hercules, USA) for TaqMan assay ...
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bioRxiv - Pathology 2023Quote: Protein samples were denatured using buffer containing Tris-carboxyethyl phosphine hydrochloride (20x XT-Reducing Agent, BioRad-1610792) and separated based on molecular weight by electrophoresis in 4-12% gradient gels (Criterion™ ...
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bioRxiv - Immunology 2022Quote: The purity and quality of the proteins were characterized at all stages of the purification process using 4%-20% SDS–PAGE (Bio-Rad, Catalog number 4561096) and 3%-12% Bis-Tris gel (ThermoFisher ...
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bioRxiv - Microbiology 2020Quote: ... concentration of total aerobic GNB and of MDR-GNB were determined by plating serial dilutions (pure, 10−2, 10−4) of initial faeces or EA sample onto Drigalski agar (Bio-Rad) with or without 1mg/L cefotaxime ...
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bioRxiv - Bioengineering 2024Quote: ... Cell density and viability were measured prior to cell plating using trypan blue and an automated cell counter (TC20, Bio-Rad). Cells were tested monthly for mycoplasma by MycoStrip (Invivogen ...
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bioRxiv - Neuroscience 2020Quote: ... Blots were exposed to developing agent (Supersignal Femto Dura, Pierce) before analysis with a ChemiDoc MP System (BioRad).
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bioRxiv - Microbiology 2020Quote: ... Colonization of SEΔΔΔ was quantified by dilution plating and counting colony forming units (CFU) on SASelect plates supplemented with 100 μg/mL D-alanine (BioRad, Hercules, CA). All experiments were done in triplicate.
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bioRxiv - Immunology 2021Quote: ... The clear supernatant was supplemented with 0.5nM nickel sulphate before being loaded onto the Bio-Scale Mini Profinity IMAC Cartridge (Bio-Rad, UK). The elution was carried out at a flow rate of 2.5 ml/min with a gradient elution of 0.05-0.25M imidazole over 60 min.
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bioRxiv - Biophysics 2023Quote: ... Solubilized protein was cleared by ultracentrifugation at 46,400 g and purified by metal affinity chromatography using a Nickel-charged EconoFit Nuvia IMAC column (Bio-Rad, USA). Protein was eluted in 20 mM HEPES ...
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bioRxiv - Biochemistry 2024Quote: ... The 6xHis-tagged G3BP1-GFP protein was purified by loading the supernatant into a nickel-nitrilotriacetic acid (Ni-NTA) column (BioRad, India) pre-equilibrated with buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were quantified by Pierce Reducing Agent Compatible BCA Protein Assay and run on 4-20% TGX gels (Biorad). Membranes were probed with anti-FLAG M2 antibody (Sigma ...
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bioRxiv - Immunology 2023Quote: ... Plates were washed (previously described) and 100 μL of diluted mouse serum (PBST wash buffer, 0.1% Biorad Blocking Agent) was added in duplicate and incubated for one hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: Spatial and temporal control of delivery of pharmacological agents was achieved using agarose beads (100-200 mesh, Bio-Rad)8,21 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The clarified lysate was loaded with a peristaltic pump (Hei-FLOW 06, Heidolph, Germany) on prepacked nickel-NTA columns (5 mL Nuvia IMAC Ni-Charged, Biorad, USA-CA) that were equilibrated before with buffer A ...
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bioRxiv - Cell Biology 2024Quote: ... each cell lysate was incubated with 15 µl of a nickel-charged resin suspension (Profinity™ IMAC Ni-Charged Resin, cat. no. 156-0131, Bio-Rad) for 2 hours and 20 minutes at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Fbxo42 was purified by His-tag using a resin containing nickel [Profinity™ IMAC (Immobilized-Metal Affinity Chromatography) Ni-Charged Resin (cat. no. 156-0131, Bio-Rad)] ...
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bioRxiv - Genetics 2021Quote: ... AUF1 and TP53 samples were prepared in a 1x Bio-Rad XT sample buffer with a 1x XT reducing agent (Biorad). Samples were boiled at 95 degrees Celcius for 5 minutes before loading on SDS-page gel.
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bioRxiv - Biophysics 2021Quote: MDCK cells stably expressing QUEEN-37C were obtained by transfecting MDCK II cells with pT2MP.EF-QUE37C vector together with pCS-TP transposase vector (Kawakami et al, 2007) using TransFectin Lipid Agent (Bio-RAD). The transfected cells were cultured under 1 μg/ml puromycin condition ...
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bioRxiv - Biophysics 2021Quote: ... folding was quenched by taking 10 μL of sample and adding it to 10 μL of 2x Laemmli SDS gel-loading buffer without reducing agent (BioRad), vortexed and stored at 4°C until analysis ...
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bioRxiv - Biophysics 2023Quote: ... Then the sample was cleared by centrifuge and the supernatant was mixed with 2x SDS-loading buffer (without any reducing agents) and analyzed by SDS-PAGE and in-gel fluorescence (Chemidoc, BioRad).
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bioRxiv - Molecular Biology 2020Quote: ... Samples were heated with LDS buffer and reducing agent before running on an SDS-PAGE gel and transferred onto a PVDF membrane (Bio-Rad). The membranes were blocked and then probed using target-specific antibodies ...
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bioRxiv - Plant Biology 2020Quote: ... Liposomes were eluted from the beads by boiling at 95°C for five minutes after the addition of 10x sample reducing agent (Nupage, NP0009) and 4x Laemmli sample buffer (Biorad, #1610747).
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bioRxiv - Microbiology 2020Quote: ... The lysates were boiled for 5 min at 95°C in 1x XT sample buffer containing 1x XT reducing agent (both from Bio-Rad) and resolved on 4-12% Bis-Tris XT precast gels (Bio-Rad) ...
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bioRxiv - Biochemistry 2020Quote: ... After 24 h incubation at 4 °C the chelating agent was removed using a desalting column (Econo-Pac 10DG from Bio-Rad), pre-equilibrated with 20 mM Hepes buffer ...
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bioRxiv - Microbiology 2023Quote: ... before destaining with Tris buffered saline Tween-20 (TBST) and incubation for 1 hour with blocking buffer (TBST with 50 g/L blocking agent (Bio-Rad, 1706404)) ...
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bioRxiv - Neuroscience 2020Quote: ... 20 μg samples were boiled in gel loading buffer containing β-mercaptoethanol as a reducing agent and loaded onto a 12% or “AnyKD” precast polyacrylamide gel (Bio-Rad 4569033) for western blot analysis ...
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bioRxiv - Neuroscience 2023Quote: ... 20µg samples were boiled in gel loading buffer containing β-mercaptoethanol and sodium lauryl sulfate as a reducing agent and loaded onto AnyKD precast polyacrylamide gels (Bio-Rad 4569033) for protein separation ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the total lysates (20-30 µg/lane) were added with a solution to reduce the disulfide bridges (Reducing Agent 20X; Bio-Rad, Milan, Italy) and Laemmli Sample Buffer 4X (Bio-Rad ...
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bioRxiv - Immunology 2024Quote: ... protein bands were visualized using Western Lightning ECL Pro detection agent (Revvity, Cat# NEL120001EA) and capturing chemiluminescence on a ChemiDoc™ Imaging System (BioRad, Cat# 12003153).
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bioRxiv - Biochemistry 2020Quote: 300-mesh in-house carbon-coated copper grids were glow discharged using BioRad E5100 SEM coating system modified in-house for glow discharging grids (BioRad Microscience Ltd, Hertfordshire, UK) in low vacuum (<200 Pa ...
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bioRxiv - Biochemistry 2021Quote: ... All final samples were boiled in 30 µL NuPAGE LDS sample buffer and sample reducing agent for 5 min and loaded on Mini-PROTEAN® TGX Stain-Free™ Gels (Bio-Rad laboratories, Hercules, CA, USA). Electrophoresis was performed at 300 V for 3 min using the BIO-RAD Mini-PROTEAN® Tetra System ...