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Citations for Bio-Rad :
51 - 100 of 1820 citations for PEGSSDA 2 x 0.5 mL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... 2.5×106 Jurkat T cells were electroporated with 2 μg minigene constructs using 220 V and 1,000 mA (Gene pulser X,Bio-Rad). After 24 h incubation ...
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bioRxiv - Biochemistry 2022Quote: ... The other two lanes were incubated with 1xTris/glycine/SDS buffer for 15 min and then incubated with 2 x Laemmli sample buffer (BioRad) for 15 min ...
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bioRxiv - Microbiology 2023Quote: ... 10 min), centrifuged (1,000 x g, 2 min) and loaded on 10% or 4–20% SDS-PAGE gel (Bio-Rad). Following electrophoresis (60 min ...
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bioRxiv - Plant Biology 2023Quote: ... Reverse transcription reaction was conducted using 0.5 µg of total RNA and 2 µl of iScript™ Reverse Transcription Supermix (Bio-Rad) in 10 µl reaction volume ...
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bioRxiv - Synthetic Biology 2024Quote: ... was added into (Ribosome purification: 5 mL; PURE proteins purification: 2-3 mL) Econo-Pac chromatography columns (Biorad). The column was washed with 50 mL deionized Millipore water and charged with 15 mL 0.1 N Nickel sulphate solution ...
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bioRxiv - Developmental Biology 2022Quote: ... and 0.5% TEMED (Bio-Rad) for 5 min on ice and 1h at 37°C ...
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bioRxiv - Physiology 2021Quote: ... with 2 mL freshly prepared TST buffer (0.03% Tween 20 [Bio-Rad] ...
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bioRxiv - Neuroscience 2023Quote: ... 6-diamidino- 2-phenylindole (DAPI) (1 ug/mL; Bio-Rad, Cat.# 1351303) to counterstain for confocal microscopy ...
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bioRxiv - Developmental Biology 2024Quote: ... with 2 mL freshly prepared TST buffer (0.03% Tween 20 (Bio-Rad), 0.01% Molecular Grade BSA (New England Biolabs) ...
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bioRxiv - Molecular Biology 2019Quote: ... Subsequent gel exclusion chromatography of loaded liposomes over a 10 mL BioGel A-0.5 M agarose resin (BioRad 151-0140) revealed no detectable AmB in the salt volume and essentially all of the AmB was retained by liposomes ...
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bioRxiv - Molecular Biology 2020Quote: ... gels were submerged into 0.5 µg/ml ethidium bromide in water for 15 minutes and imaged with Gel Doc XR+ system (Bio-Rad). Banding pattern of Mlh1+/− samples were compared to the controls to identify the allele with and without Mlh1 promoter methylation.
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bioRxiv - Physiology 2020Quote: ... pre-stained with Ethidium bromide (0.5 μg/ml) and gel images were documented using a Bio Rad gel documentation system (BIORAD, USA). Next ...
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bioRxiv - Bioengineering 2022Quote: ... and the nanocluster size was derived by eluting 20.0 μL of the sample with PBS buffer at a flow rate of 0.5 mL/min and calibrating the chromatograph with that of the protein standards (Bio-Rad). The hydrodynamic size and zeta potential of supraclusters were determined using a Zetasizer NANO ZS-90 instrument (Malvern) ...
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bioRxiv - Biochemistry 2023Quote: ... Approximately 3 mg of concentrated protein were loaded into a Superdex200 increase 10/300 GL column using a 0.5 mL loop on a FPLC BioLogic DuoFlow (Bio-Rad) at a rate of 0.5mL/min ...
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bioRxiv - Immunology 2024Quote: 96-well flat-bottom NUNC Maxisorp plates were coated overnight at 4 °C with 0.5 mg/mL recombinant HBsAg (BIO-RAD). Plates were washed with PBS/T ...
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bioRxiv - Plant Biology 2022Quote: ... by reverse phase chromatography using a Bio-scale MT column (2 mL, BioRad). The column was packed with Macro-Prep® t-Butyl and Methyl Hydrophobic Interaction Chromatography Media ...
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bioRxiv - Biochemistry 2022Quote: ... Detergent was removed by addition of 200 mg/ml SM-2 BioBeads (BioRad) followed by incubation under slow rotation for 16 h at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... and loaded onto 2 mL Nuvia IMAC Ni-charged resin (Bio-Rad Laboratories). The resin was washed with 10 column volumes of buffer containing 5 mM imidazole ...
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bioRxiv - Molecular Biology 2022Quote: ... at a 1:5 w/w ratio for 2 hours before addition of 100 mg mL-1 Bio-Beads SM-2 resin (Bio-Rad) and then incubated overnight at 4 °C with gentle agitation to remove detergent ...
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bioRxiv - Immunology 2021Quote: ... sections were stained with 0.4 µg/mL rabbit -anti-human CD20 polyclonal antibodies (Neomarkers) and 2 µg/mL rat-anti-human CD3 antibodies (clone MCA1477, BioRad). Then ...
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bioRxiv - Cell Biology 2022Quote: ... Triton X-100 (Bio-Rad) used at 1% for 10 min at RT allowed cell permeabilization and a solution of bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2020Quote: Brain tissues were homogenized in lysis buffer and then combined with 2 x Laemelli buffer (Bio-Rad Laboratories, Inc., Hercules, CA, USA). Protein samples were then separated on 7.5 to 12.5% v/v sodium dodecyl sulfate (SDS ...
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bioRxiv - Neuroscience 2020Quote: ... brain tissues were homogenized in lysis buffer and then combined with 2 x Laemelli buffer (Bio-Rad Laboratories, Inc., Hercules, CA, USA). Protein samples were then separated on 7.5 to 12.5% v/v sodium dodecyl sulfate (SDS ...
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bioRxiv - Biophysics 2022Quote: ... Samples were centrifuged for 2 min at 12,000 x g before being separated on 8–16% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad) at 160 V for 60 min ...
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bioRxiv - Plant Biology 2020Quote: ... Reactions were performed in duplicate using the iCycler iQ Thermal Cycler with a 96 x 0.2 ml reaction module and iCycler iQ software (Bio-Rad).
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bioRxiv - Molecular Biology 2022Quote: ... Sample was injected and run on ENrich SEC 650 10 x 300 24 mL size exclusion column (Bio-Rad 7801650) using Tn5 SEC Buffer ...
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bioRxiv - Synthetic Biology 2022Quote: ... the hybrid RNA/DNA origami was run on 1.5% agarose gel in TAE buffer (75 mL were poured into a 15 cm x 10 cm gel tray, Biorad) and analysed by in-gel imaging using the specific dye DFHBI-1T as described above with few modifications due to the different gel composition (agarose instead of polyacrylamide ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was incubated for 1 h at 4°C with 0.5 mL Profinity IMAC resin (Bio-Rad Laboratories, Hercules, CA) in a buffer containing 20 mM MOPS-Na (pH 7.4) ...
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bioRxiv - Microbiology 2023Quote: ... filtered HEK293F supernatants containing the expressed proteins were flowed (0.5 -1.0 mL/min) over Econo-Column chromatography columns (Bio-Rad, CA, USA) containing 1-2 mL of agarose bound GNL (Vector Laboratories) ...
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bioRxiv - Molecular Biology 2023Quote: ... The HURP antibody was then purified by first conjugating 10 mg of HURP(462-576) antigen to 0.5 mL of Affi-gel 15 (Bio-Rad: 1536051) resin (brought up to 10 mL with PBS buffer) ...
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bioRxiv - Immunology 2024Quote: Samples were prepared in 1 x Laemmli buffer with or without 1 x dithiothreitol (Biorad). Samples were then heated for 10 min at 95 °C and loaded onto a precast NuPAGE™ 4-12 % Bis-Tris polyacrylamide gel in NuPAGE™ MES SDS running buffer (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... The clarified supernatant was filtered through a 0.4 μM filter to remove the insoluble fraction and supplemented with 30 mM Imidazole pH 7.0 immediately before loading at a flow rate of 2 mL/min onto a 5 mL Ni-NTA immobilized metal affinity chromatography (IMAC) column (Bio-Rad) equilibrated in Buffer A (50 mM Tris-Cl ...
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bioRxiv - Biochemistry 2024Quote: ... The clarified supernatant was filtered through a 0.4 μM filter to remove the insoluble fraction and supplemented with 30 mM Imidazole pH 7.0 immediately before loading at a flow rate of 2 mL/min onto a 5 mL Ni-NTA immobilized metal affinity chromatography (IMAC) column (Bio-Rad) equilibrated in Buffer A (50 mM Tris-Cl ...
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bioRxiv - Biochemistry 2021Quote: ... pH 7.5 and loaded onto a 2 ml CHT2-I hydroxyapatite column (Bio-Rad) equilibrated in 20 mM HEPES ...
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bioRxiv - Microbiology 2021Quote: ... Gels were stained with ethidium bromide (0.5 mg mL-1) and photographed using the Quantity One electrophoresis analysis system (Bio-Rad, CA, USA). Gel images were digitally normalized to a single DNA marker to reduce gel-to-gel banding pattern variability ...
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bioRxiv - Neuroscience 2022Quote: ... The embryoid media was aspirated and 15 to 20 embryoid bodies were plated per well in 6-well plates and cultured in 3 mL hematopoetic medium (2 mM GlutaMax, 1x Anti-Anti, 55 mM 2-mercaptoethanol (BioRad; Cat. No. 1610710), 100 ng/mL M-CSF ...
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bioRxiv - Biochemistry 2020Quote: ... 0.5% non-fat dry milk (Bio-Rad).
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bioRxiv - Biochemistry 2020Quote: ... 0.5% non-fat dry milk (Bio-Rad). Loaded sensors were equilibrated for 30 s in buffer ...
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bioRxiv - Microbiology 2020Quote: ... 0.5% (v/v) Bio-Lyte Ampholyte (BioRad) and trace amounts of bromophenol blue ...
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bioRxiv - Neuroscience 2022Quote: ... 0.1% Triton X-100 (Bio-Rad), in PBS] for 2h ...
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bioRxiv - Physiology 2023Quote: ... 1% Triton X-100 (Bio-Rad), and 0.5% Igepal (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2023Quote: ... and 0.1% Triton X (Biorad, USA); (2 mg propidium iodide and 2 mg RNaseA/100 mL 1X PBS) ...
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bioRxiv - Cell Biology 2022Quote: ... Triton X-100 (Bio-Rad, USA) at 0.1% ...
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bioRxiv - Cell Biology 2023Quote: ... 0.1% Triton X-100 (Bio-Rad) in PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... 60 of High Capacity NeutrAvidin slurry (Thermo PI29204) were washed three times with 1 mL 2X RIPA/1mM EDTA buffer in 2-mL chromatography columns (Bio-Rad 7326008) attached to a vacuum manifold (Promega A7231) ...
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bioRxiv - Biochemistry 2023Quote: ... Clarified lysates were added to a 2 mL bed of Ni-NTA agarose resin in a 20 mL column (Bio-Rad 7321010) equilibrated with wash buffer (50 mM Tris-HCl (pH 8) ...
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bioRxiv - Molecular Biology 2022Quote: ... Reactions were performed in total 24ul volume using 12ul of 2 x ddPCR Supermix for Probes (No dUTP) (Bio-Rad Laboratories, catalog no. 1863024), 900nM of target primer pairs ...
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bioRxiv - Biochemistry 2019Quote: ... with or without CLEC-2 antibody (3 μg/ml final concentration; Bio-Rad, Oxford, UK). The plate was incubated in the dark for the indicated time and the reaction was stopped by addition of 200 μl 1% ice-cold formalin ...
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bioRxiv - Biochemistry 2022Quote: ... Mass calibration was done by using 2 mg/ml BSA standard (Bio-Rad, cat.5000206). PBS pH 7.4 was used as isocratic buffer ...
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bioRxiv - Microbiology 2021Quote: ... Detergent was removed by incubation with 0.6 g/ml Bio-Beads SM-2 (Bio-Rad) at 4 °C for 2 hours ...