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Citations for Bio-Rad :
51 - 100 of 1110 citations for Octreotide acetate impurity C since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: APRT2-Ntag and APRT2-Ctag were buffer exchanged into a volatile buffer (200 mM ammonium acetate, pH 7.4) using a Micro Bio-Spin 6 device (Bio-Rad). Samples were loaded into pulled glass capillaries prepared in house ...
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bioRxiv - Immunology 2021Quote: ... before buffer exchange into 500 mM ammonium acetate solution (pH 7.5) using a Micro Bio-Spin 6 column (Bio-Rad).
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bioRxiv - Biochemistry 2022Quote: ... were exchanged from size exclusion buffer into 2 M ammonium acetate pH 7.4 via a single-pass through a Bio-Spin P-6 column (Bio-Rad). Buffer-exchanged protein was loaded into gold-coated glass capillaries (1.0 mm OD/0.75 mm inner diameter with filament) ...
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bioRxiv - Neuroscience 2022Quote: ... equal amounts of protein (2.5 or 10 µg) were separated by SDS-PAGE on 4-12% Bis-Tris or 3-8% Tris acetate gels (Criterion XT, BioRad) and transferred to nitrocellulose using TransBlot Turbo (BioRad) ...
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bioRxiv - Molecular Biology 2024Quote: All proteins except for the full-length HSPB7 were buffer-exchanged into 200 mM ammonium acetate (pH 6.9) using biospin (Bio-Rad); full-length HSPB7 was buffer-exchanged into 1 M ammonium acetate (pH 6.9) ...
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bioRxiv - Biochemistry 2024Quote: AcrAS was buffer exchanged into a volatile solution (100 mM ammonium acetate, pH 6.0) using a centrifugal exchange device (Micro Bio-Spin 6, Bio-Rad) according to manufacturer’s instructions native MS experiments were performed on a Synapt G2-Si mass spectrometer (Waters) ...
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bioRxiv - Molecular Biology 2024Quote: ... 200 mM ammonium acetate at the desired pH using a centrifugal buffer exchange device (Micro Bio-Spin 6, Bio-Rad). Stock solutions of lipids were prepared from chloroform/methanol solution by evaporating aliquots of known volume in a SpeedVac ...
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bioRxiv - Cancer Biology 2024Quote: ... the plate was held at 4 °C until the lid temperature cooled to 37 ºC and could be stored at 2 °C to 8 °C overnight before analysis on the QX600 Droplet Reader (Bio-Rad Laboratories, California, USA).
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bioRxiv - Neuroscience 2020Quote: ... containing the crude synaptosomes was resuspended and processed for western blotting in NP-40 lysis buffer as above with the following modifications: equal amounts of protein per sample (unboiled) were separated on 3-8% Tris-Acetate XT gel (Bio-Rad) in XT Tricine running buffer (Bio-Rad ...
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bioRxiv - Biochemistry 2020Quote: ... the buffer of the purified DNAJB6 was exchanged into 10 mM ammonium acetate pH 7 solution using Micro Biospin P6 centrifuge columns (Bio-Rad) and protein concentration determined using a NanoDrop spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... acetate concentrations were measured using a high-performance liquid chromatograph (HPLC) equipped with an Aminex HPX-87H column (BioRad, United States) maintained at 59°C and coupled to an ultraviolet detector at 210 nm (Waters United States) ...
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bioRxiv - Neuroscience 2023Quote: ... The pellets were resuspended with 2 % SDS and loaded onto a cellulose acetate membrane assembled in a slot blot apparatus (Bio-Rad). The membrane was washed with 0.2% SDS and retained SDS-insoluble mHTT aggregates were detected with mouse anti-polyQ antibody (Millipore ...
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bioRxiv - Bioengineering 2024Quote: ... PCR products were mixed 1:6 with a loading dye and run on an agarose gel (1%) in Tris-acetate-EDTA buffer supplemented with 0.2 µG/mL of Ethidium Bromide (Biorad, Hercules, California). DNA was visualized under a short-wavelength UV light to confirm the lack of amplification for Heat-Killed Microbiome PMA-treated in comparison to the Heat-Killed Microbiome control (Supplementary Figure 2).
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bioRxiv - Evolutionary Biology 2024Quote: Protein samples were buffer exchanged into 200mM ammonium acetate using either a centrifugal buffer exchange device (Micro Bio-Spin P-6 Gel, Bio-Rad) or a dialysis device (Slide-A-Lyzer MINI Dialysis Unit ...
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bioRxiv - Developmental Biology 2021Quote: ... denaturation at 95°C by 0.5°C in a Real-Time PCR apparatus (BioRad, USA). The genes of interest in this study are listed in supplementary Table 3 ...
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bioRxiv - Biophysics 2024Quote: ... the solution was heated and slowly cooled from 80°C to 4°C (ramp rate of -1°C per 5 s) in a standard thermocycler (Bio-Rad CFX96 Real-Time System) prior to each experiment.
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bioRxiv - Biochemistry 2022Quote: ... from 20°C to 95 °C in C1000 Thermal Cycler CFX96 Real-time System (Bio-Rad). Tm was determined as a local minimum of the first derivative of the raw fluorescence as function of temperature with software Bio-Rad CFX Manager 3.1.
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bioRxiv - Physiology 2023Quote: ... 120 min at 37 °C and 5 min at 85 °C in a Thermocycler (T100, BioRad). For real-time amplification ...
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bioRxiv - Cell Biology 2021Quote: ... 3–8% Tris-acetate gels (Life Technologies. Proteins were transferred to nitrocellulose membrane by semi-dry transfer (Trans-Blot Turbo transfer system; Bio-Rad) or ...
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bioRxiv - Genomics 2021Quote: ... PCR products were resolved using a 2% agarose tris-acetate-EDTA gel and a 177 bp band was visualized using the ChemiDoc™ imaging system (Bio-Rad).
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bioRxiv - Plant Biology 2022Quote: ... Blots were washed with TBST buffer three times for 10 min each time and inserted between acetate transparency sheets and imaged on a ChemiDoc Touch Imaging System (Bio-Rad Laboratories) using Immobilon western chemiluminescent HRP substrate (MilliporeSigma ...
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bioRxiv - Bioengineering 2021Quote: ... were desalted and buffer exchanged into 10 mM ammonium acetate using a spin column (Micro Bio-Spin™ P-6 Gel Columns, Bio-Rad). Octylamine was added to aliquots of NCB solution at a concentration of 0.1% (v/v) ...
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bioRxiv - Cell Biology 2022Quote: ... To verify the generation of single chain antibodies we prepared samples of whole and reduced antibodies with NuPAGE LDS Sample Buffer (4x) and run them in NuPAGE™ 7% Tris-Acetate Protein Gels (Bio-Rad) in denaturing ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... was homogenized in 10 mM sodium acetate buffer (500 μL, pH 6.5) and filtered through Bio-spin 6 column (Bio-Rad, Hercules, CA) prewashed with 10 mM sodium acetate buffer (pH 6.5 ...
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bioRxiv - Plant Biology 2020Quote: ... and stopped at each of the above-mentioned time points by loading an 150 µl aliquot of the reaction mixture to an anion exchange resin column (acetate form, 100-200 mesh, Dowex AG1-X8 Resin, Bio-Rad, UAS). The resin column was previously equilibrated five times using 150 mM sodium acetate (pH=7.5) ...
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bioRxiv - Molecular Biology 2024Quote: ... at 4°C (Bioruptor Pico, BioRad). Then ...
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bioRxiv - Cell Biology 2023Quote: ... 62 cycles (10′′ 65 °C + 0.5 °C) in the SYBR-Green-Cycler IQ5 detection protocol (Biorad CFX384), performed in 384-well plates (Merck) ...
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bioRxiv - Biochemistry 2021Quote: ... and their respective complexes with Hap40 protein were buffer-exchanged into 150 mM aqueous ammonium acetate (pH=7.5) by using P-6 Bio-Spin gel filtration columns (Bio-rad, Veenendaal, the Netherlands). The protein’s resulting concentration was estimated to be ~2-5 μM before native MS analysis ...
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bioRxiv - Biophysics 2023Quote: Proteins samples were kept on ice and buffer exchanged into 200 mM ammonium acetate buffer at pH 7 using Micro Bio-spin columns with either a 6 or 30 kDa cutoff (Bio-Rad, Hercules, CA). Buffer exchanged samples were then diluted to a working concentration of 10 μM before analysis ...
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bioRxiv - Genetics 2023Quote: ... Bromophenol blue buffer was loaded on 1.0 mm thick Criterion XT Tris acetate (poly-acrylamide) gels with a linear resolving gel gradient of 4-12% (Biorad; Lunteren, The Netherlands). Gels were run at 75 V (0.07 A ...
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bioRxiv - Biochemistry 2024Quote: Protein samples (S100A9ec and S100A9in) were buffer exchanged into 200 mM ammonium acetate at pH 7 using Micro Bio-spin P6 columns (Bio-Rad, Hercules, CA). Buffer exchanged samples were then diluted to a working concentration of 10 μM ...
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bioRxiv - Cancer Biology 2022Quote: ... and 4 minutes at 72°C and then final extension at 72°C for 10minutes (MyCycler, Bio-rad). After the reaction ...
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bioRxiv - Immunology 2022Quote: ... 42 °C for 50 min and 85 °C for 5 min in a thermal cycler (Bio-Rad, USA). Synthesized cDNA was kept at −80 °C till further use ...
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bioRxiv - Cell Biology 2023Quote: ... Precision Plus Protein Western C ladder (BioRad) and Precision protein Streptactin-HRP conjugate (BioRad ...
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Biallelic loss-of-function OBSCN variants predispose individuals to severe, recurrent rhabdomyolysisbioRxiv - Genetics 2021Quote: Frozen muscle biopsies were homogenised in modified Laemmli sample buffer as described previously.37 Samples were run in Bio-Rad Criterion 3–8 % tris-acetate gradient gels (Bio-Rad Laboratories, CA, USA) at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... The temperature was varied 25 - 95 °C in 0.5 °C increments by using an iCycle IQ5 real-time PCR system (BioRad). Relative fluorescence was measured using excitation and emission wavelengths of 580 nm and 623 nm ...
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bioRxiv - Biochemistry 2021Quote: ... MDH2 aliquots were heated to 42 °C for 10 min then returned to 37 °C in a thermocycler (BioRad), while native samples were maintained at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... The fluorescence was measured at 512 nm along a temperature gradient from 20 to 100 °C (0.5°C increase every 10 s) in the CFX ConnectTM real-time thermocycler (BioRad) with the Cfx MaestroTM software ...
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bioRxiv - Molecular Biology 2024Quote: ... Melt curve between 65°C and 70°C was analysed using Bio-Rad Precision melt software (Bio-Rad, CA) to identify sex genotypes ...
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bioRxiv - Synthetic Biology 2024Quote: ... Subsequently the samples were heated to 75 ◦C for 1 min and then cooled down to 25 ◦C with a temperature gradient of −0.5 ◦C per min in a thermocycler (BioRad). For bundling ...
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bioRxiv - Plant Biology 2024Quote: ... 10 s at 60 °C and 30s at 72 °C with a CFX Connect Real-Time system (Bio-rad). ΔΔCt was used to analyze the gene expression data.
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bioRxiv - Biochemistry 2022Quote: 5 µL aliquots of the CRISPR complex solution were buffer exchanged into 100 mM ammonium acetate using Biospin P-6 gel columns (Bio-Rad Laboratories Inc., Hercules, CA) prior to native mass spectrometry ...
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bioRxiv - Biochemistry 2022Quote: LiRecT protein was buffer exchanged into 100 mM ammonium acetate pH 7 (unadjusted) using Micro BioSpin P6 spin columns (Bio-Rad Laboratories, Hercules, CA, USA). All ssDNAs were dialyzed into 100 mM ammonium acetate with Pierce 96-well microdialysis devices with 3.5K MWCO (Thermo Fisher Scientific) ...
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bioRxiv - Systems Biology 2021Quote: ... followed by 15 s at 95 °C and 60 s at 58 °C for 40 cycles using Biorad CFX384 thermocycler (Biorad). The mRNA levels of genes encoding cytokine expression were normalized relative to the mean levels of the housekeeping gene and compared using the 2−ΔΔCt method as described previously2 ...
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bioRxiv - Systems Biology 2020Quote: ... reverse transcription for 20 mins at 46 °C and inactivation for 1 min at 95 °C) in a thermal cycler (C1000 Touch, Biorad). TaqMan Fast Advanced Master Mix (Applied Biosystems ...
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bioRxiv - Systems Biology 2020Quote: ... 40 cycles of PCR denaturing for 5 secs at 95 °C and annealing/extending for 30 secs at 55 °C) in real-time PCR system (CFX384 Touch, Biorad).
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bioRxiv - Microbiology 2020Quote: ... 73105b and 73106f pseudotyped viruses were incubated at temperatures from 37°C to 50°C for 60 minutes using temperature gradient on PCR thermal cycler (BioRad). Pseudoviruses were then aliquoted in a 96-well culture plate ...
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bioRxiv - Biophysics 2022Quote: ... The protein sample was incubated at 40°C and 80 °C respectively for 1 h in a PCR cycler (BioRad) with a heated lid to prevent evaporation ...
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bioRxiv - Neuroscience 2022Quote: ... then denatured for 5 min at 95°C and annealed by gradual cooling down at −0.1 °C/sec using a PCR thermocycler (BioRad T100). Annealed oligos were subsequently inserted into BbsI-digested vector pX330-U6-Chimeric_BB-CBh-hSpCas9 (Addgene #42230) ...
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bioRxiv - Biophysics 2022Quote: ... The mixture was then heated up to 95°C for 5 minutes and cooled down to 30°C at the rate of 1°C per minute using a thermal cycler (BioRad).