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Citations for Bio-Rad :
51 - 100 of 1641 citations for Neuronal acetylcholine receptor subunit alpha 5 NACHRA5 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2019Quote: ... 96-well Alpha™Unit cycling block and low-profile PCR stripes (Bio-Rad Laboratories GmbH, Muenchen, Germany) were utilized ...
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bioRxiv - Immunology 2020Quote: Human plasma was analysed using the human 27-plex cytokine panel (Bio-Rad) according to manufacturer’s instructions and contained the following targets ...
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bioRxiv - Cell Biology 2019Quote: ... Immunostaining for the vitronectin receptor (VNR) was with anti-CD51/61 (clone 23C6, 1:400; Bio-Rad, Oxford, UK) and for cathepsin K with a rabbit polyclonal antibody (3368-100 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Expressions optical density were determined and presented as ratio against GAPDH with the help of alpha imager (Bio-Rad). All the expressions were checked in triplicate (Table 1).
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bioRxiv - Immunology 2020Quote: ... We used FITC-conjugated mouse anti-pig CD4 alpha (clone MIL17, Bio-Rad AbD Serotec, Puchheim, Germany, 1:20) and Alexa Fluor 647-conjugated mouse anti-pig CD8α (clone 76-2-11 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were blocked for 1hr in PBS with 1mg/ml and 0.1% triton and then stained overnight with anti-alpha Tubulin antibody (1:100, MCA78G, BioRad). Cells were then washed ...
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bioRxiv - Plant Biology 2020Quote: ... The His-tagged fusion protein was purified using a Ni-NTA column (Bio-Rad, USA). Protein was eluted in elution buffer [500 mMNaCl ...
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bioRxiv - Plant Biology 2023Quote: ... and then evaluated by immune-decoration analysis using a monoclonal His-antibody (AbHis, Bio-rad). Pichia transformants expressing FHS-CELLOX was already available (Scortica et al. ...
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bioRxiv - Immunology 2024Quote: ... The membrane was then incubated with mouse anti-His antibody (catalog number #6200203, Bio-Rad), diluted 1:1,000 in the blocking solution and incubated overnight at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... Detection of His-tagged proteins was performed with the ClarityTM Western ECL substrate (Biorad, USA) and using a Versadoc (Biorad ...
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bioRxiv - Molecular Biology 2022Quote: ... human TGFβ1 (PHP143B, Bio-Rad).
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bioRxiv - Immunology 2022Quote: ... human TGFβ1 (PHP143B, Bio-Rad), anti-IL11 antibody (X203 ...
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bioRxiv - Cell Biology 2024Quote: ... Human (BioRad-assay ID qHsaCID0017132) and ACTB - PrimePCR™ SYBR® Green Assay ...
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bioRxiv - Cell Biology 2024Quote: ... Human (BioRad-assay ID qHsaCED0036269). The cycles to threshold was measured for each well ...
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bioRxiv - Cell Biology 2024Quote: ... Human (BioRad-assay ID qHsaCED0044963) INSR - PrimePCR™ SYBR® Green Assay ...
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bioRxiv - Cell Biology 2023Quote: ... The reaction mixture was separated on SDS-PAGE and the phosphorylated receptor was autoradiographed for image analysis with the Quantity One program (BioRad) on phosphor screens.
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bioRxiv - Systems Biology 2021Quote: ... Quantitative analyses of blotted hCDKL5 and His-Neuropinilin were carried out with Image Lab software (BioRad) and the volumetric yield was derived considering the final biomass concentration (OD600 ...
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bioRxiv - Microbiology 2020Quote: ... The plates were washed and incubated with mouse anti-His (Bio-Rad MCA-1396; 1:1000) followed by horseradish peroxidase (HRP)-conjugated goat anti-mouse secondary antibody (MerckMillipore AP124P ...
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bioRxiv - Microbiology 2023Quote: ... Binding of VHHs was detected using a mouse anti-HIS-tag antibody (Biorad, MCA1396, 1/2000) and an AF647 conjugated donkey anti mouse IgG antibody (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... Colorimetric (standards) and chemiluminescent detection (His-tagged proteins) were performed using the ChemiDoc Imager (Bio-Rad).
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bioRxiv - Cancer Biology 2020Quote: ... spliced IL6 receptor and gp130 expression was assessed by PCR using the MJ Research Peltier Thermal Cycler Tetrad (Bio-Rad, Germany) with the following primers ...
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bioRxiv - Immunology 2021Quote: ... rat anti-human CD8 (Bio-Rad), rat anti-human Granzyme B (eBioscience) ...
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bioRxiv - Genetics 2023Quote: ... Human (Bio-Rad, 10031243, ID: dHsaCP1000002). Reference assay for 3.1 kb deletion and 3.1 kb inversion assays ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit anti-human CX3CR1 (Biorad, AHP1589), mouse anti-human CD68 (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... anti-human CX3CR1 (Bio-Rad, AHP566). Mouse TNFα ELISA Kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... 20μL reactions were prepared in triplicate in 96-well white plates (Alpha Laboratories, UK) consisting of 10μL iTaq™ Universal SYBR® Green Supermix (BioRad), 1μL 20x TaqMan gene expression assay ...
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bioRxiv - Cell Biology 2022Quote: ... and imaged either with MultiImage Light Cabinet using FluorChem 8900 software (Alpha Innotech Corporation, San Leandro, CA, USA) or Gel Doc XR+ System with Image Lab Software (Bio-Rad). Coomassie staining with Colloidal Blue staining kit (Thermo Fisher Scientific Cat# LC6025 ...
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bioRxiv - Microbiology 2021Quote: ... and then incubated for at least 16 hours at 4°C with either mouse monoclonal anti-His (1:2,000; AD1.1.10, Biorad) or mouse anti-RNA pol (1:5,000 ...
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bioRxiv - Biochemistry 2022Quote: ... and the protein was passed through Hi-Q anion exchange column (Bio-Rad Laboratories, Hercules, CA, USA) to remove minor nucleic acid contamination ...
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bioRxiv - Plant Biology 2021Quote: Purified His and GST fusion proteins or GST alone (500 ng) were blotted onto nitrocellulose membrane (BioRad). The nitrocellulose membrane was rinsed with TBS-T buffer (10 mM Tris-HCl at pH 7.4 ...
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bioRxiv - Microbiology 2020Quote: ... Eluents were collected and incubated with 50μL of packed Ni-NTA resin (Bio-Rad His-Pur 88222) at room temperature with nutating for 30 min ...
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bioRxiv - Molecular Biology 2022Quote: ... and cultures were induced with IPTG and His-MBP-ORC2 was purified on Ni-NTA beads (BioRad). Purified protein was injected into rabbits for serum generation and collection (Cocalico Biologicals) ...
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bioRxiv - Biophysics 2022Quote: ... His-tagged σs was added to phosphorylated/unphosphorylated RssB in Micro Bio-Spin Chromatography columns (Bio-Rad) before removing 6μL of the input for SDS-PAGE analysis and adding 40μL of prepared HisPur Ni-NTA resin (Thermo Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... and human proinflammatory cytokines were quantified using the Bio-Plex Pro™ Human Inflammation Panel 1 (BioRad). Fluorescence was read using a MAGPIX System (Luminex ...
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bioRxiv - Plant Biology 2020Quote: ... The reactions were carried out in a thermal cycler (Single Block Alpha Unit, DNA Engine®, Bio-Rad Laboratories, Berkeley, CA, USA) with an annealing temperature of 55 °C ...
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bioRxiv - Microbiology 2020Quote: ... was used to purify the C-terminal 6X-His EfgA with 1 mL Ni-NTA columns (Bio-Rad). Columns were equilibrated with 10 mL of Buffer A at 1 mL/min prior to loading lysates ...
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bioRxiv - Biochemistry 2022Quote: ... and eluted from the C4 reverse-phase column (Hi-Pore reversed-phase column, 4.6 × 250 mm, Bio-Rad) by the acidic acetonitrile gradient.
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bioRxiv - Microbiology 2023Quote: ... His-tagged proteins in the cleared lysate were purified using Profinity™ IMAC Nickel Charged Resin (Bio-Rad) and eluted in 50 mM sodium phosphate buffer with 300 mM NaCl pH 8 and 300 mM imidazole (Fischer Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... monoclonal rat anti-human CD3 (Bio-Rad), rat anti-mouse B220/CD45R (clone RA3-6B2 ...
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bioRxiv - Immunology 2023Quote: ... goat-anti-human 1:3000 (Biorad /1721050); goat-anti-rabbit 1:2000 (CST / 7074) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... 3 μL of the resuspended membrane pellets containing the receptors were run alongside membrane pellet of uninjected oocyte on 4–15% precast gradient TGX Stain-Free™ gels (Bio-Rad Laboratories) for 34 min at 200 V ...
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bioRxiv - Neuroscience 2020Quote: ... The amount of surface expressed receptors was detected by adding 60 µL wash buffer and 20 µL HRP substrate (Bio-Rad, 170-5060) per well ...
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bioRxiv - Cell Biology 2023Quote: ... mouse monoclonal anti-MF20 (DSHB, Cat# MF 20, dilution 1:20) rabbit polyclonal anti-Calcitonin Receptor (Bio-RAD, Cat# AHP635, dilution 1:2000), chick anti-GFP (abcam ...
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bioRxiv - Biophysics 2024Quote: ... 5% acrylamide (BIORAD), 0.1% SDS ...
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bioRxiv - Bioengineering 2021Quote: ... Membranes were stained overnight at 4°C with a 1:1000 diluted mouse anti-his primary antibody (MCA1396, RRID:AB_322084, Bio-Rad) and then for 1 hour at room temperature with a 1:4000 diluted rabbit anti-mouse HRP secondary antibody (SouthernBiotech Cat# 6170-05 ...
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bioRxiv - Molecular Biology 2023Quote: ... His-tagged recombinant protein was purified using Ni-NTA purification with the help of Ni-charged resins (Bio-Rad). Purified protein was further used for kinase assay ...
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bioRxiv - Immunology 2021Quote: ... sections were stained with 0.4 µg/mL rabbit -anti-human CD20 polyclonal antibodies (Neomarkers) and 2 µg/mL rat-anti-human CD3 antibodies (clone MCA1477, BioRad). Then ...
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bioRxiv - Microbiology 2020Quote: ELISA plates were coated overnight at 4°C with 0.1 µg/mL of mouse anti-human IgG (human CH2 domain with no cross-reactivity to rhesus macaque IgG; clone R10Z8E9; BioRad) and then blocked for 2 hours ...
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bioRxiv - Immunology 2023Quote: ... IL-17A titres in human serum samples were quantified using a multiplex cytokine panel (Bio-Plex Pro Human Cytokine Assay, BioRad) and a LuminexCorp Luminex 100 machine as per the manufacturer’s instructions.
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Tracing production instability in a clonally-derived CHO cell line using single cell transcriptomicsbioRxiv - Cell Biology 2021Quote: ... The recombinant Human IgG1 Kappa (Biorad, cat.no. HCA192) was used as a positive control ...