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Citations for Bio-Rad :
51 - 100 of 108 citations for N Acetyl Tizanidine d4 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: ... Relative NKA and NHE protein abundance (n = 5 per treatment) was quantified using the Image Lab software (version 6.0.1; BioRad) and normalized by the protein content in each lane.
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bioRxiv - Molecular Biology 2021Quote: ... PCR product was purified by preparative agarose gel electrophoresis (Quantum Prep Freeze ‘N Squeeze spin column, Bio-Rad) and magnetic beads (Agencourt AMPure XP ...
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bioRxiv - Biophysics 2020Quote: ... we purified the DNA FluoroCubes by extraction and centrifugation in Freeze ’N Squeeze columns (BioRad Sciences, 732-6165). Prior to extraction ...
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bioRxiv - Biochemistry 2021Quote: ... PCR product was purified by preparative agarose gel electrophoresis (Quantum Prep Freeze ‘N Squeeze spin column, Bio-Rad) and magnetic beads (Agencourt AMPure XP ...
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bioRxiv - Molecular Biology 2024Quote: ... the entire samples were applied to Hybond-N using a Bio-Dot Microfiltration Apparatus (Bio-Rad Laboratories, Inc.). After UV crosslinking ...
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bioRxiv - Biochemistry 2023Quote: ... reduced N- and O-glycans samples were desalted using a cation exchange resin (AG 50W-X8; Bio-Rad). N-glycomics of BLCA cell lines was performed similarly after immobilization of membrane protein extracts in a hydrophobic PVDF membrane (MAIPS4510 ...
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bioRxiv - Molecular Biology 2021Quote: ... the entire samples were applied to Hybond-N using a Bio-Dot® Microfiltration Apparatus (Bio-Rad Laboratories, Inc.). After UV crosslinking ...
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bioRxiv - Cell Biology 2021Quote: ... The N-ter (pI 5.5) and C-ter (pI 9.9) peptides were conjugated to Affi-Gel-10 (Bio-rad) agarose beads for affinity-purification ...
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bioRxiv - Bioengineering 2024Quote: ... Structures were also evaluated by TEM to confirm structural stability following gel purifification using Freeze ’N Squeeze (Bio-Rad) gel extraction columns.
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bioRxiv - Neuroscience 2024Quote: ... Protein levels were visualized using ECL-based autoradiography film system (Super RX-N, Fujifilm) or ChemiDocTM Imaging system (BioRad) and analyzed using Gel Analyzer plugin from ImageJ (Fiji) ...
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bioRxiv - Biophysics 2024Quote: ... the excised band was placed in a Freeze ‘N Squeeze™ DNA Gel Extraction Spin Column (BioRad, 732-6165) and centrifuged for 3 min at 12,000 rcf at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... Ten micrograms of total RNA were separated on a 1% agarose gel containing 1X 3-(N-morpholino)propanesulfonic acid (MOPS) buffer and 2.2 M formaldehyde and transferred to a Zeta-probe membrane (Bio-Rad) by capillary transfer in 20X SSC buffer (3 M NaCl ...
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bioRxiv - Molecular Biology 2022Quote: ... Ten micrograms of total RNA were separated on a 1% agarose gel containing 1X 3-(N-morpholino)propanesulfonic acid (MOPS) buffer and 2.2 M formaldehyde and transferred to a Zeta-probe membrane (Bio-Rad) by capillary transfer in 20X SSC buffer (3 M NaCl ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA samples were fragmented for 10 minutes on ice in 0.2 N NaOH and purified on Bio-Spin P30 columns (Bio-Rad). Biotin-labelled RNA was then purified using MyOne Streptavidin C1 Dynabeads (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... The RNA was transferred to a Hybond-N+ Nylon transfer membrane (Cytiva) with a Trans-Blot SD blotting apparatus (BioRad) followed by crosslinking at 0.12 J/cm2 in a CX-2000 UV crosslinker (Analytik Jena) ...
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bioRxiv - Biochemistry 2023Quote: ... 2mM DTT and 0.02% n-dodecyl-β-D-maltopyranoside [DDM]) buffer after transferring into Econo columns (Biorad, Cat. no. 7372512). Thrombin at concentration 1unit µL-1 was added to the resin slurry at 1:1 (dry resin:cleavage buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Heads (N = 15 for each genotype) were decapitated and homogenized in 100 µl 2X Laemmli Sample Buffer (BIO-RAD 1610737) containing 5% of 2-Mercaptoethanol (Sigma-Aldrich M3148) ...
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bioRxiv - Immunology 2023Quote: ... Bio-Plex SARS-CoV-2 Wuhan-1 strain RBD and N coupled beads (Bio-Rad, Cat Nos. 12015406 and 12014773), and Bio-Plex Pro Human IgA detection antibody (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: The DNA was denatured using 0.5 M NaOH and 1.5 M NaCl and equal amounts were loaded onto a Hybond N + nitrocellulose membrane (GE Biosciences) using the Bio-Dot apparatus (Bio-Rad). Membranes were washed once with denaturing buffer and wash buffer (3× SSC) ...
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bioRxiv - Developmental Biology 2022Quote: ... GEM-RT was performed in a C1000 Touch Thermal cycler with 96-Deep Well Reaction Module (Bio-Rad; P/N 1851197): 53°C for 45 min ...
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bioRxiv - Bioengineering 2019Quote: Proliferation of MSCs was evaluated by determining the total cell count per well (n=3 wells/treatment/time-point) using a TC20 Automated Cell Counter (Bio-Rad).
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bioRxiv - Cancer Biology 2022Quote: ... The reaction was stopped using glacial acetic acid and the N-glycans desalted sequentially using strong cation exchange (AG 50W X8, Bio-Rad), C18 and porous graphitised carbon (PGC ...
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bioRxiv - Microbiology 2022Quote: ... This panel had been tested negative for SARS-CoV-2 nucleocapsid protein expression using Bio-Plex Pro Human IgG SARS-CoV-2 N/RBD/S1/S2 4-Plex Panel (Bio-rad). The second serum panel (n=29 ...
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bioRxiv - Microbiology 2020Quote: ... The protein concentration of the supernatant was determined by Bradford measurement according to the manufacturer’s instructions (Bio-Rad, cat n° 5000006).
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bioRxiv - Genomics 2021Quote: ... RNAs were then transferred to a nitrocellulose membrane (GE Amersham HybondTM-N+) using a wet transfer system (Trans-Blot cell, Bio-Rad) at 100 V for 2 h at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... The resolved genomic DNA was then transferred to the positively charged nylon membranes (Hybond-N+, Amersham Pharmacia Biotech) using a model 785 vacuum blotter system (Bio-Rad). The Bar amplified fragment (Table 1 ...
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bioRxiv - Molecular Biology 2022Quote: ... The libraries were eluted from the gel by freezing the gel pieces at −20°C for 30 min in Freeze ‘N Squeeze DNA gel extraction spin columns (Bio-Rad) and recovering the solution by immediate centrifugation of the columns at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... This panel had been tested negative for SARS-CoV-2 nucleocapsid protein expression using Bio-Plex Pro Human IgG SARS-CoV-2 N/RBD/S1/S2 4-Plex Panel (Bio-rad). The second panel consisted of 29 sera collected from individuals 1 month after BA.5-bivalent-booster of Pfizer or Moderna vaccine ...
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bioRxiv - Microbiology 2022Quote: ... DNA/protein complexes were transferred to a Hybond-N+ positively charged nylon membrane (Amersham Pharmacia Biotech) using an electroblot transfer system (Bio-Rad) and cross-linked with short-wave UV light in a GS gene linker UV chamber (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: ... with 10 μg of plasmid DNA expressing LpAsp-GFP or LpCht-GFP as well as pTrex-n-eGFP using a Gene Pulser X cell electroporator (Bio-Rad) and cuvette (2 mm gap) ...
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bioRxiv - Genomics 2024Quote: ... Samples were then run on a 2% agarose gel with 1x SYBR Safe for 2 hours at 120 V before gel extraction with a Freeze ‘N Squeeze column (Bio-Rad). After extraction ...
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bioRxiv - Microbiology 2024Quote: Total RNA was loaded onto 8 to 12% PAGE–7 M urea gels and transferred to Hybond-N+ (GE) membranes using a Trans-Blot Turbo Transfer system (Bio-Rad) in 1X TBE ...
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bioRxiv - Biochemistry 2023Quote: ... to saturate the N-terminal metal binding site,10 prior to buffer exchange using a centrifugal buffere exchange device (Bio-Spin, Bio-Rad) into 200 mM ammonium acetate supplemented with 2 x CMC of C10E5 ...
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bioRxiv - Molecular Biology 2023Quote: ... and A6p (Table S1) were run on agarose gels and purified using Freeze ‘N Squeeze DNA gel extraction spin columns (Bio-Rad). For manual sequencing ...
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bioRxiv - Microbiology 2023Quote: ... 12 % SDS-PA gels were fixed with fixation solution (40 % ethanol, 10 % acetic acid) o/n and stained in Flamingo fluorescent protein dye (Bio-Rad) for up to 6 h and imaged with the ChemiDoc (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... The purified RNA was separate on a 1.2% agarose-formaldehyde denaturing gel and was then transferred to a nylon membrane (Hybond-N+, Cytvia) in 10X SSC buffer using a model 785 vacuum blotter (Bio-Rad). RNA was cross-linked to the membrane using an ultraviolet (UV ...
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bioRxiv - Microbiology 2023Quote: ... targeting SARS-CoV-2 gRNA and N protein sgRNA were used for the quantification by RT-qPCR using iTaq Universal SYBER Green Supermix (Bio-Rad) and normalised to β-actin using 2-ΔΔCT.
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bioRxiv - Microbiology 2023Quote: ... This panel had been tested negative for SARS-CoV-2 nucleocapsid protein expression using Bio-Plex Pro Human IgG SARS-CoV-2 N/RBD/S1/S2 4-Plex Panel (Bio-rad). The second panel consisted of 20 sera from individuals who were previously infected by SARS-CoV-2 vaccinated with 2-4 doses of parental mRNA vaccine ...
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bioRxiv - Microbiology 2024Quote: Total RNA was loaded onto 8 to 12% PAGE–7 M urea gels and transferred to Hybond-N+ (GE) membranes using a Trans-Blot Turbo Transfer system (Bio-Rad) in 1X TBE ...
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bioRxiv - Molecular Biology 2024Quote: ... 500ng of RNA has been diluted in 250 μl of TE low EDTA buffer and applied to pre-equilibrated positively charged nylon membrane (Amersham Hybond-N+ # RPN203B) on Slot blot apparatus (Biorad # 1706542) by gentle vacuum ...
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bioRxiv - Immunology 2024Quote: ... from colonic samples of DR3.IL17A-/- (n = 5) and DR3 mice (n = 5) were reverse transcribed to cDNA using the High-Capacity iScript cDNA synthesis kit (Bio-Rad). qPCR reactions were then carried out in triplicate using 50 ng of cDNA and the Applied Biosystems Power SYBR Green PCR Master Mix (Applied Biosystems) ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples (n = 80 embryos / lane) were run on a precast SDS-PAGE gel (4-20% MP TGX Stain-Free Gel, Bio-Rad 4568093), with a Precision Plus Protein WesternC Stds ladder (Bio-Rad 1610376) ...
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bioRxiv - Neuroscience 2021Quote: ... 20 μL 5 N NaOH solution was added to and the absorbance was read at 405 nm with a reference filter of 620 nm by iMark Microplate Reader S/N 20255 (Bio-Rad, USA).
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bioRxiv - Synthetic Biology 2019Quote: ... at which point waste acrylamide was separated from the aqueous slats solution with Freeze N’ Squeeze spin columns (Bio-Rad, 732-6166). The DNA slats were further purified by precipitation in isopropanol ...
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bioRxiv - Cell Biology 2019Quote: Total RNA was extracted from cardiac cultures after 4 days on either soft or stiff PDMS (n=4) using the AurumTM Total RNA Mini Kit (Bio-Rad Laboratories). Libraries were constructed by the Purdue Genomics Core Facilities according to standard protocols using TruSeq Stranded mRNA Library Prep Kit (Illumina) ...
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bioRxiv - Synthetic Biology 2020Quote: ... Target bands were excised on a blue light transilluminator and purified with a Quantum Prep® Freeze ‘N Squeeze spin column (Bio-Rad). The eluate was further purified with a magnetic bead clean-up (Agencourt® AMPure® XP ...
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bioRxiv - Genomics 2020Quote: ... Purified DNA was slot blotted onto a Hybond N+ membrane with the help of a dot-blot apparatus (Bio-Rad, 170-6545) and subsequently hybridized with a biotin-labeled (TTAGGG)3 probe synthesized by Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... The protein concentration of the supernatant was determined by Bradford measurement (64) according to the manufacturer’s instructions (Bio-Rad, cat n° 5000006).
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bioRxiv - Physiology 2023Quote: ... in duplicates in 384-qPCR plates (n = 5 plates) and qPCR were performed with a Biorad instrument (CFX-384, Biorad, Hercules, USA). We used Recombination Activating Gene 1 (RAG1 ...
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bioRxiv - Immunology 2023Quote: Serum binding antibody assays were performed to evaluate IgG responses to SARS-CoV-2 nucleocapsid and ancestral spike S2 domains and RBD using the Bio-Plex Pro Human SARS-CoV-2 IgG (N, S2, RBD) 4-Plex Panel serology assay (Bio-Rad 12014634) as previously described (8).