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Citations for Bio-Rad :
51 - 100 of 1534 citations for Caspase 9 CASP9 Antibody Pair since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... the primer pairs (Table 2; exception: S. umbla) were used to create EvaGreen-based droplet dPCR assays using the AutoDG (Bio-Rad) for droplet generation ...
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bioRxiv - Immunology 2020Quote: Densitometric analysis of cleaved caspase 3 immunoblots from three independent experiments were performed using the VersaDoc Imaging System (Bio-Rad) and analyzed with ImageJ 1.52p Fiji package software (https://imagej.net/Fiji) ...
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bioRxiv - Physiology 2020Quote: ... Gene expression was assessed using commercially available Taqman primer/probe sets (Thermo Fischer) or specific oligonucleotide pairs (Table 1) and SYBR® Green supermix (Bio-Rad). Relative expression of the target genes was calculated with the comparative Ct method [43] using glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Neuroscience 2021Quote: ... Master mixes containing primer pairs against FIBCD1 or B2M (Beta-2-microglobulin, housekeeping gene) were prepared with iTaq Universal SYBR Green Supermix (Bio-Rad #1725122). The mastermixes were dispensed into each well containing cDNA and incubated on ice for 15 minutes to allow the cDNA to dissolve ...
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bioRxiv - Developmental Biology 2021Quote: ... following by 6 cycles of pre-amplification with pooled 47 pairs of MTE primers designed for NanoString CodeSet (SsoAdvanced™ PreAmp Supermix, Cat# 1725160, Bio-Rad); amplified dsDNA was analysed using the NanoString expression protocol.
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bioRxiv - Immunology 2024Quote: ... RT-qPCR was conducted using HiScript II One Step qRT-PCR SYBR Green Kit (Q221-01, Vazyme) with gene-specific primer pairs on a CFX Connect Real-Time PCR Detection System (Bio-Rad Laboratories). The thermal cycling protocol was as follow ...
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bioRxiv - Microbiology 2021Quote: ... PCR conditions followed the general Bio-Rad recommendations.9 Data was analyzed with QuantaSoft_v1.7 software (Bio-Rad) including automatic Poisson correction.9,11
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bioRxiv - Developmental Biology 2019Quote: ... and gene-specific primers (Supplementary Table 9) on a CFX384 Touch Real-Time PCR Detection System (BioRad). TBP (TATA-binding protein ...
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bioRxiv - Systems Biology 2023Quote: ... then cells stained for analysis by flow cytometry 9 or 10 days after dCas9 infection (BioRad ZE5). In some cases ...
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bioRxiv - Physiology 2019Quote: ... PCR was performed with 20ng of cDNA and the corresponding primer pairs using an iCycler Real-Time Detection System (BioRad Laboratories, Hercules, CA). The relative quantity of mRNA was determined using the comparative Ct method and expression levels of P2Y2 receptor mRNA were normalized to ribosomal 18S expression.
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bioRxiv - Immunology 2022Quote: ... and 1 µl of respective primer pairs (200 nM final concentration) were mixed and distributed into 96-well qPCR plate (Bio-Rad; cat. #12001925). Droplets were generated in an Automated Droplet Generator (Bio-Rad) ...
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bioRxiv - Biochemistry 2021Quote: ... The conjugated beads were then packed into a 9 mL glass column (Bio-Rad laboratories, Hercules, CA, USA). The dimeric APPI (15 mL ...
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bioRxiv - Molecular Biology 2021Quote: ... 300nM final volume of each forward and reverse primer pair, 5 μL SYBR Green Supermix (50% total volume of reaction, rxn) (Bio-Rad Inc. Hercules, CA), and nuclease-free water (to make a final reaction volume of 10 μL) ...
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bioRxiv - Immunology 2021Quote: ... Equal amounts of protein samples were separated by 9% SDS-PAGE gel and transferred onto nitrocellulose membranes (Bio-Rad). The following primary antibodies were used ...
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bioRxiv - Plant Biology 2021Quote: ... The following components were added to a reaction tube: 9 μL of iQ SYBR Green Supermix solution (Bio-Rad), 1 μL of 5 mM specific primers ...
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bioRxiv - Bioengineering 2020Quote: ... EG depletion was monitored using on an Aminex HPX-87H ion exclusion column (300 mm x 7.8 mm, particle size 9 μm; Bio-rad). The column was maintained at 40°C and samples were isocratically eluted using 0.014 N H2SO4 at a flow rate of 0.55 ml min-1 and read on a refractive index detector (RID) ...
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bioRxiv - Immunology 2022Quote: ... Lysates containing 40 μg of proteins were analyzed by SDS-PAGE on 9-13% gels and proteins were transferred onto nitrocellulose membranes (BioRad). The blots were blocked in non-fat milk diluted in tris-buffered saline (TBS ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was isolated from 6-week-old rosette leaves and bolting flower buds of Col-0 and one of the RPF2-atp1 (RPF2-atp1-9) transformed lines (T3) with PureZol reagent (BioRad). Three independent libraries for each genotype were made from total RNA treated with 250 ng of Turbo DNase (Ambion ...
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bioRxiv - Synthetic Biology 2022Quote: ... All reagents except the double-stranded beacon were first assembled in a total volume of 9 μL and run at 37 °C in a CFX96 real-time PCR detection system (Biorad). Both WT SpCas9 and SpCas9-NG are highly active at this temperature ...
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bioRxiv - Genomics 2020Quote: ... were seeded per well in 6-well plates (2 ml per well). 24 hrs later cells were transfected with siRNA (Supp. Table 9) using SilentFect (Bio-Rad) in biological triplicates ...
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bioRxiv - Biochemistry 2021Quote: ... The cells were replenished with compounds every 24 h and counted on days 1-9 using automated cell counter (Bio-Rad). KYSE-520 cells were plated onto 96-well plates (500 cells per well ...
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bioRxiv - Biochemistry 2021Quote: ... RQN > 9) were prepared using the NEBNext Ultra II Directional RNA Library Kit for Illumina with NEBNext rRNA depletion (Bio-Rad). Protocols were followed as per the manufacturer’s guidelines except that 40X adaptor dilutions were utilized ...
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bioRxiv - Microbiology 2021Quote: ... Sugar consumption and the metabolite production were analyzed using HPLC Prominence (Shimadzu) with ion exclusion column Aminex ®HPX-87H (300×7.8mm×9 μm) (Bio-RAD), isocratically eluted at 60 °C with 5 mM sulfuric acid flow rate of 0.6 mL.min-1 ...
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bioRxiv - Cancer Biology 2022Quote: ... The immunoprecipitated proteins from three treatments were mixed and either denatured by 9 M Urea/20 mM HEPES buffer or mixed with 2x Laemmli Sample Buffer (1610737, Bio-Rad) and separated by SDS-PAGE to analyze individual fractions ...
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bioRxiv - Physiology 2023Quote: ... Samples were run 16 h at 9 °C at 4 V with a 1–10 running ratio in TBE buffer using CHEF-DRII system (Bio-Rad). After the run ...
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bioRxiv - Genomics 2023Quote: Pooled blood cells from 9 adults were counted and their viability was assessed using an automatic cell counter (Bio-Rad TC20) with Trypan Blue staining ...
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bioRxiv - Genetics 2023Quote: ... China) using TBE (65 mM Tris-HCl, 27 mM boric acid, 1 mM EDTA, pH 9; Bio-Rad, Hercules, CA, USA) as the buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNA was generated by reverse transcription of up to 9 ng RNA from each sample using iScript Reverse Transcription Supermix for RT-qPCR (BioRad, 1708841). mRNA expression was determined on a StepOnePlusTM Real-Time PCR System (Thermo Fisher ...
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bioRxiv - Molecular Biology 2019Quote: ... Denatured protein extracts were resolved using 4-9% SDS-PAGE (4–15% Mini-PROTEAN® TGX™ Precast Protein Gelscat# 4561083, Biorad) and then transferred to a nitrocellulose membrane.
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bioRxiv - Cancer Biology 2020Quote: ... a ddPCR reaction was prepared (13 μl mastermix and 9 μl cfDNA) and subsequently ran on a QX200 ddPCR system according to protocol (Bio-Rad Laboratories). Data analysis was performed using QuantaSoft v1.7.4.0917 (Bio-Rad Laboratories) ...
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bioRxiv - Molecular Biology 2019Quote: ... Denatured proteins (20-40 µg) were electrophoresed in 9% SDS-PAGE gels or MiniPROTEAN® TGX™ 4-15% Precast gels (BIORAD), transferred onto a nitrocellulose membrane and probed with specific antibodies ...
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bioRxiv - Plant Biology 2019Quote: ... A Bio-Rad Aminex HPX-87H (300 x 7.8 mm, 9 μm particle size) column with a Micro-Guard Cation H guard column (Bio-Rad, Watford, UK) was used to achieve separation with an isocratic gradient of 5 mM sulfuric acid ...
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bioRxiv - Cancer Biology 2021Quote: ... Ribonucleoprotein (RNP) complexes were made at a 9:1 ratio of sgRNA:Cas9 (90pmol:10pmol) in Gene Pulser® Electroporation Buffer (Bio-Rad, 1652677) and incubated for 10 min at RT ...
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rpoB, a promising marker for analyzing the diversity of bacterial communities by amplicon sequencingbioRxiv - Microbiology 2019Quote: ... The 16S rRNA gene was amplified (the primer sequences are indicated in Additional file 9) in a Bio-Rad thermocycler (Bio-Rad, USA). PCR products were analyzed by agarose gel electrophoresis ...
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bioRxiv - Developmental Biology 2019Quote: ... using 9% SDS polyacrylamide gels and then transferred to a 0.2µM polyvinylidene fluoride (PVDF) membrane over 10 minutes (Bio-Rad Trans-Blot Turbo). Membranes were blocked with 5% skim milk in 0.05% TBS-T for 1 hour ...
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bioRxiv - Physiology 2023Quote: ... 0.03% bromophenol blue) containing 9% beta-mercaptoethanol and separated by Mini-Protean TGX gel 4 - 15% (BioRad, stain-free gels #4568084, 4561035) and blotted onto Amersham Hybond-P PVDF membrane ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Protein lysates were boiled in loading buffer [1:9 ratio of 2-mercaptoethanol:4x Laemmli Sample Buffer (Cat.#1610747; Bio-Rad Laboratories)] for 10 min ...
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bioRxiv - Neuroscience 2022Quote: After 9-13 days in culture, organotypic hippocampal slices were transfected biolistically with gene gun (McAllister, 2000) using gold beads (Bio-Rad, 1.6 µm) coated with plasmids ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Sixty μg of each protein sample was run on 9% polyacrylamide gels and transferred to a nitrocellulose membrane with the Turbo Blotdry blot system (Biorad, Hercules, CA, USA). After transfer ...
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bioRxiv - Bioengineering 2020Quote: ... quantification was performed using a Bio-Rad Fast Acid Analysis HPLC Column (100 x 7.8 mm, 9 μm particle size; CAT#: #1250100, Bio-Rad Laboratories, Inc., Hercules, CA) at 65 °C ...
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bioRxiv - Bioengineering 2020Quote: ... Chromatographic separation was performed using a Rezex Fast Acid Analysis HPLC Column (100 x 7.8 mm, 9 μm particle size; CAT#: #1250100, Bio-Rad Laboratories, Inc., Hercules, CA) at 55 °C ...
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bioRxiv - Immunology 2022Quote: ... cell debris was removed by centrifugation and clear supernatants were diluted 10-fold and subjected to anion exchange chromatography on gravity fed columns using AG-1 9 8 resin (formate form, 100–200 mesh size, Bio-Rad Laboratories, Hercules, CA). Fractions containing inositol mono ...
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bioRxiv - Synthetic Biology 2020Quote: ... Chromatographic separation was performed using a Bio-Rad Fast Acid Analysis HPLC Column (100 x 7.8 mm, 9 μm particle size; CAT#: #1250100, Bio-Rad Laboratories, Inc., Hercules, CA) at 65 °C ...
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bioRxiv - Synthetic Biology 2020Quote: ... Chromatographic separation was performed using a Bio-Rad Fast Acid Analysis HPLC Column (100 x 7.8 mm, 9 μm particle size; CAT#: #1250100, Bio-Rad Laboratories, Inc., Hercules, CA) at 65 ℃ ...
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bioRxiv - Physiology 2020Quote: ... The same blood samples were used to analyze the fluctuations in cytokine profiles using Bio-plex (GI 23-Plex and GII 9-Plex: Bio-Rad, Hercules, CA, USA), in accordance with the manufacturers’ instructions.
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bioRxiv - Cell Biology 2021Quote: ... coli antibodies (1:100, Bio-Rad Antibodies, 4329-4906), followed by DyLight 650-conjugated donkey anti-rabbit IgG antibodies (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... secondary antibodies (1:10000; 1:50000 for anti-H3 antibody, BioRad) in 5% milk/PBST at RT ...
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bioRxiv - Microbiology 2020Quote: ... The following antibodies were used: mouse anti-rat CD43 antibody (BioRad), rabbit polyclonal Surfactant C (Proteintech) ...
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bioRxiv - Neuroscience 2023Quote: ... secondary antibodies (1:10000; 1:50000 for anti-Gapdh antibody, BioRad) in 5% milk/PBST at RT ...
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bioRxiv - Neuroscience 2023Quote: ... secondary antibodies (1:10000; 1:50000 for anti-H3 antibody, BioRad) in 0.1% Tween-20 in 1x PBS (PBS-T ...