Labshake search
Citations for Bio-Rad :
51 - 100 of 1316 citations for Aminomethylphosphonic Acid 13C 99%;15N 98%;Methylene D2 98% 100 Ug Ml H2O since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Physiology 2023Quote: ... . Proteins (30-50 ug) were prepared by adding laemmli sample buffer (Bio-Rad, 1610747) and heated at 950C for 5min ...
-
bioRxiv - Neuroscience 2022Quote: ... the reaction was stopped by a final methanol-chloroform-H2O precipitation and resuspended in laemmli buffer (Bio-Rad) for WB analysis.
-
bioRxiv - Biophysics 2019Quote: ... Residual Ca2+ from this solution was removed by passing 100 mL of TLK buffer over 5 g of pH-neutralized Chelex 100 resin (BioRad) in a column three times at a flow rate of ~3 mL/min ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Samples were eluted with 6 mM H2SO4 at a flow rate of 0.6 ml/min from an Aminex HPX 87H organic acid analysis column (300 by 7.8 mm) (BioRad). We recorded the areas for all noticeable peaks associated with detected metabolites (glucose ...
-
bioRxiv - Bioengineering 2024Quote: ... and subsequently destained in MilliQ H2O overnight before being imaged on a Gel Doc XR+ Molecular Imager (BIO-RAD). In-gel TAMRA fluorescence of quenchbodies following SDS-PAGE was analysed on an Amersham Typhoon Biomolecular Imager (Cytiva) ...
-
bioRxiv - Microbiology 2022Quote: ... RNA (1 ug total) was reverse transcribed using iScript cDNA Synthesis Kit (Bio-Rad, 1708891) and diluted to 100 ul in ddH2O for subsequent qPCR analyses ...
-
bioRxiv - Developmental Biology 2020Quote: ... and 20-40 ug was loaded onto 4-15% pre-cast gels (Bio-Rad 17000546) and run using the Bio-Rad Mini-PROTEAN Tetra Cell system ...
-
bioRxiv - Neuroscience 2023Quote: ... 1 ug of RNA was used for cDNA synthesis using iScript cDNA synthesis kit (Biorad), according to the manufacturer’s guidelines ...
-
bioRxiv - Biophysics 2020Quote: ... The supernatant was applied to a 100 mL DEAE-Affigel Blue (Bio-Rad) column equilibrated with buffer A (25 mM Tris-HCl (pH 7.4) ...
-
bioRxiv - Immunology 2022Quote: ... the bound fraction was suspended in 0.1 ml 10% Chelex-100 (Bio-Rad), vortexed ...
-
bioRxiv - Genomics 2021Quote: ... They were then boiled for 5 minutes at 99°C after the addition of Laemmli buffer (Cat. #1410737, Biorad). Proteins were separated by SDS-PAGE and transferred to a membrane with a Trans Blot TURBO (Biorad) ...
-
bioRxiv - Genetics 2020Quote: ... for 2 × 1 h washes at room temperature and then stored overnight in MilliQ H2O before being visualized for myosin bands on a Chemi Doc MP (Biorad). Membranes were blocked at room temperature for 2 h using LI-COR blocking buffer with TBS (LI-COR Biosciences) ...
-
bioRxiv - Microbiology 2022Quote: ... Several 10-fold dilutions of each purified DNA were prepared in nuclease-free H2O and added to a reaction mixture containing ddPCR EvaGreen Supermix (Biorad). Forward (5′-GAATACATTCACATTGACCAATCAGAA-3′ ...
-
bioRxiv - Plant Biology 2023Quote: ... diluted (1 μL in 50 μL milliQ-H2O) and deposited into sample wells onto a nitrocellulose membrane disposed into a 96-well Bio-Dot microfiltration apparatus (BioRad) previously soaked with in TBS (0.02 M Tris-HCl pH 7.5 ...
-
bioRxiv - Molecular Biology 2024Quote: ... the gel was rinsed briefly with Milli-Q H2O and scanned using the gel imaging system (Bio-Rad ChemiDoc XRS+) in the Cy5 filter channel ...
-
bioRxiv - Biochemistry 2021Quote: ... cDNA was synthesized from 1 ug of RNA using iScript cDNA Synthesis Kit from Bio-Rad. PCR reaction was carried out using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad ...
-
bioRxiv - Genetics 2022Quote: ... 1 ug of RNA was reverse-transcribed using iScript RT Supermix (Bio-Rad 1708841, Hercules, CA). qPCR was performed using SsoAdvanced SYBR Green Super Mix (Bio-Rad 1725270 ...
-
bioRxiv - Genetics 2020Quote: Protein homogenates (10-20 ug) were separated on gradient TGX gels (Bio-Rad, #4568084 or #4568095) and blotted onto PVDF membranes ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... Approximately 2 ug of biotinylated HA was heated in non-reducing Laemmli buffer (Bio-Rad, #1610737) at 95°C for 5 minutes ...
-
bioRxiv - Plant Biology 2023Quote: ... 1 ug of RNA were used for cDNA synthesis with iScript cDNA Synthesis Kit (Bio-Rad). qPCR was performed with iQ SYBR Green Supermix (Bio-Rad ...
-
bioRxiv - Cell Biology 2022Quote: ... A total of 50 ug of WCL was combined with 4X Laemelli Sample Buffer (BioRad, 1610747) to a final concentration of 1X sample buffer ...
-
bioRxiv - Cancer Biology 2023Quote: ... cDNA was obtained from 1 ug RNA using iScript cDNA synthesis kit (Bio-Rad, Hercules, CA). CBX7 and GAPDH qPCR was performed using SYBR Green Supermix (Bio-Rad ...
-
bioRxiv - Cancer Biology 2024Quote: ... Approximately 20 ug protein was analyzed by 4%–15% gradient TGX Precast polyacrylamide gel (Bio-Rad), transferred onto a PVDF membrane (Biorad Transblot Turbo system ...
-
bioRxiv - Developmental Biology 2020Quote: ... boiled at 99 °C for 10 min and supernatants were loaded on 4–12% Criterion XT Bis–Tris precast gel (Biorad). The gel was fixed with 40% Methanol and 10% acetic acid and then stained for 1 hour using colloidal coomassie dye G-250 (Gel Code Blue Stain ...
-
bioRxiv - Developmental Biology 2021Quote: ... boiled at 99 °C for 10 min and supernatants were loaded on 4–12% Criterion XT Bis–Tris precast gel (Biorad). The gel was fixed with 40% methanol and 10% acetic acid and then stained for 1 hour using colloidal coomassie dye G-250 (Gel Code Blue Stain ...
-
bioRxiv - Immunology 2022Quote: ... One ml of sterile Bio-Gel P-100 polyacrylamide beads (Bio-Rad, 150-4174) in PBS (2% w/v ...
-
bioRxiv - Cell Biology 2023Quote: ... loaded with a 100 mg/ml Biogel P-10 Gel (Bio-Rad, Hercules, CA) solution ...
-
bioRxiv - Microbiology 2020Quote: ... Each cDNA sample was diluted 1:15 in nuclease free H2O prior to qPCR analysis using specific primers and the iTaq Universal SYBR green mastermix (Bio-Rad). Primers for qPCR were designed using Primer3 software and include ...
-
bioRxiv - Cancer Biology 2021Quote: ... Bound SRB dye was dissolved by adding 10 mM Tris-H2O to wells and OD was measured at 510nM with an iMARK microplate reader (Bio-Rad).
-
bioRxiv - Cell Biology 2023Quote: ... cDNA was diluted 1:20 in RNase-free H2O and 3 µl dilution were used for qRT-PCR analysis using iTaq Universal SYBR Green (Bio-Rad) according to manufacturer’s recommendation ...
-
bioRxiv - Bioengineering 2020Quote: ... quantification was performed using a Bio-Rad Fast Acid Analysis HPLC Column (100 x 7.8 mm, 9 μm particle size; CAT#: #1250100, Bio-Rad Laboratories, Inc., Hercules, CA) at 65 °C ...
-
bioRxiv - Bioengineering 2020Quote: ... Chromatographic separation was performed using a Rezex Fast Acid Analysis HPLC Column (100 x 7.8 mm, 9 μm particle size; CAT#: #1250100, Bio-Rad Laboratories, Inc., Hercules, CA) at 55 °C ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Chromatographic separation was performed using a Bio-Rad Fast Acid Analysis HPLC Column (100 x 7.8 mm, 9 μm particle size; CAT#: #1250100, Bio-Rad Laboratories, Inc., Hercules, CA) at 65 °C ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Chromatographic separation was performed using a Bio-Rad Fast Acid Analysis HPLC Column (100 x 7.8 mm, 9 μm particle size; CAT#: #1250100, Bio-Rad Laboratories, Inc., Hercules, CA) at 65 ℃ ...
-
bioRxiv - Microbiology 2024Quote: ... The samples were then treated according to the instruction of the beads and the protein complexes were then eluted by incubating at 99°C for 5 minutes with Laemmli Sample Buffer (1610747, Bio-Rad).
-
bioRxiv - Microbiology 2024Quote: ... 30-50 μg of total protein was boiled at 99°C for 5 minutes with the addition of Laemmli Sample Buffer (1610747, Bio-Rad) containing 1% 2-Mercaptoethanol in a total volume of 60-80 μl ...
-
bioRxiv - Cell Biology 2021Quote: ... 15 ug of protein were separated by 4-20% Mini Protean TGX SDS-polyacrylamide gels (Bio-Rad) at 120 V for 1 h and transferred onto 0.2 um PVDF membranes (Bio-Rad ...
-
bioRxiv - Cell Biology 2022Quote: ... cDNA was prepared from 1 ug of purified RNA using the iScript cDNA Synthesis Kit (Bio-Rad) as stated in the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2021Quote: ... 10 ug of total protein was loaded per lane onto a 12% SDS-PAGE gel (Bio-Rad), which was run at 100 V for 2 hours ...
-
bioRxiv - Immunology 2022Quote: ... 10-30 ug total protein were resolved by SDS-PAGE and transferred to PVDF membranes (Bio-Rad). Primary antibody incubations were done overnight with antibodies diluted in 3% BSA in TBS-T (Tris-buffered saline/Tween 20) ...
-
bioRxiv - Molecular Biology 2022Quote: ... cDNA was synthesized from 1 ug of RNA using the iScript cDNA Synthesis Kit (BioRad #170-8891). Reverse Transcriptase Quantitative PCR (RT-qPCR ...
-
bioRxiv - Bioengineering 2024Quote: Purified antibodies (0.5 ug each) were denatured by adding 4X LaemmLi sample buffer (Bio-Rad, cat.: 1610747) and 2-mercaptoethanol and heating at 100°C for 5 minutes ...
-
bioRxiv - Cell Biology 2023Quote: 600 μL of DMEM GlutaMax™ supplemented with 10% FBS, 20 μg/mL gentamycin, and either 100 ng/mL recombinant mouse Monocyte chemoattractant protein-1 (MCP-1, CCL2) (Bio-Rad, PMP35) or vehicle control (ddH2O) ...
-
bioRxiv - Biochemistry 2020Quote: A 10% Tris/Glycine/SDS Buffer solution was prepared with 100 mL buffer (Bio-Rad) in 900 mL tissue culture grade water ...
-
bioRxiv - Microbiology 2021Quote: ... 0.5 mL of 100 μM protein samples was loaded on ENrich SEC 70 column (BioRad) in a buffer containing 50 mM phosphates (pH 7.4) ...
-
bioRxiv - Biophysics 2022Quote: ... Detergents were removed by incubation with 100 mg/ml Bio-Beads SM-2 (Bio-Rad) for 1h at 23°C ...
-
bioRxiv - Microbiology 2023Quote: Overnight cultures in TSB were diluted 100-fold into 1 mL of Chelex (Bio-Rad)- treated TSB with the addition of 25 μM zinc acetate ...
-
bioRxiv - Cell Biology 2019Quote: ... The bicinchoninic acid assay (Bio-Rad) was used to measure protein concentration from the cell lysates and 20 μg of protein was loaded onto 8% SDS gels and electrophoresed ...
-
bioRxiv - Molecular Biology 2022Quote: ... Protein extract (4 ug per lane) was separated on a 10% TGX Stain-Free polyacrylamide gel (Bio-Rad) and transferred to a nitrocellulose membrane ...
-
bioRxiv - Systems Biology 2022Quote: Cell extracts (15 ug) were resolved by SDS-PAGE using 4-15% TGX precast gels (Bio-Rad #4561086) with tris/glycine/SDS buffer and transferred onto PVDF membranes using the Bio-Rad Mini-PROTEAN Electrophoresis System ...