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Citations for Bio-Rad :
901 - 950 of 1100 citations for Recombinant Mouse Fabp4 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were further incubated in peroxidase-conjugated anti-mouse or anti-rabbit secondary antibody (1:5000) (BioRad, Hercules, CA, USA) for 1 hr at room temperature ...
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bioRxiv - Immunology 2020Quote: ... Supernatants of lung homogenates used for cytokine analysis were stored at −80 with 1x Pierce Proteinase Inhibitor until testing using Bio-Plex Pro Mouse Cytokine Th17 Panel A with additional GM-CSF and IL-12p70 Singleplex sets on a Bio-Plex200 Platform (BioRad). Cytokine levels were normalized against total protein measured by Bradford assay.
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bioRxiv - Immunology 2021Quote: ... were added to each well and incubated with cross-absorbed anti-mouse IgG HRP polyclonal antibody (diluted 1:10,000) (STAR117P, Bio-Rad) for 2h at RT on a Multiscan FC Plate Reader (Thermo Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... The following primary antibodies were used for western-blotting at 1/1,000 dilution: anti-V5 clone SV5-Pk1 (mouse, monoclonal, BioRad, #MCA1360), anti-Rpb3 clone 1Y26 (mouse ...
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bioRxiv - Biochemistry 2022Quote: ... We utilized colorimetric detection to visualize the MBP-Vpu band(s) on the membrane after WB transfer: Primary mouse anti-histidine tag antibody (BioRad) and secondary goat anti-mouse IgG antibody conjugated to Alkaline Phosphatase (BioRad ...
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bioRxiv - Cell Biology 2022Quote: ... The following antibodies were used for immunoblotting: Talin-1 (1:5000 dilution, Mouse monoclonal, Clone 97H6; Bio-Rad, Cat# MCA4770), YAP (1:1000 dilution ...
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bioRxiv - Microbiology 2022Quote: ... Cytokines in the supernatants were then measured by using Bio-Plex Pro mouse cytokine 23-plex assay (Bio-Rad Laboratories). Likewise BALF from control and immunized mice was used to measure cytokines.
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bioRxiv - Microbiology 2022Quote: ... Membranes were rinsed 3x for 15 minutes in 1X TBST and incubated in the proper secondary antibody (Goat anti-mouse BioRad #170-6516 or Goat anti-rabbit BioRad #170-6515 ...
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bioRxiv - Neuroscience 2022Quote: ... Peripheral plasma cytokine levels were determined using Bio-Rad Pro mouse cytokine multiplex commercial kits (Bio-Rad, Montreal, QC, Canada). Also ...
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bioRxiv - Microbiology 2024Quote: ... Plates were washed three times before the addition of HRP-conjugated anti-mouse IgG (H+L; Bio-Rad; 170-6516) at 1:3,000 and anti-mouse IgG2b (ab97250 ...
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bioRxiv - Microbiology 2023Quote: ... The proteins were visualized using the secondary antibody Anti-Mouse IgG-HRP using Clarity Max™ Western ECL Substrate (BioRAD).
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bioRxiv - Cell Biology 2023Quote: ... Additional antibodies used for the infected ALI cultures include: mouse anti-RSV Fusion protein (1:100, Cat# MCA490, Bio-Rad), rabbit anti-Cleaved Caspase-3 (Asp175 ...
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bioRxiv - Microbiology 2023Quote: ... fed nymph and mouse bladder samples using the iScript Reverse Transcription supermix kit according to the manufacturer’s instructions (Bio-Rad). Parallel cDNA reactions were carried out in the absence of reverse transcriptase ...
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bioRxiv - Molecular Biology 2023Quote: The procedure was performed according to the instructions of Bio-Plex Pro Mouse Cytokine 23-plex (Bio-Rad, CA, USA). Serum was diluted 4 times with Sample Diluent ...
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bioRxiv - Genetics 2023Quote: ... Blots were washed in 1x TBST with mild agitation before incubation with horseradish peroxidase-conjugated secondary antibodies diluted in blocking buffer (α- mouse 1:10,000 (170-6516; BioRad), α-rabbit 1:10,000 (170-6515 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and ddPCR was conducted as previously described (48, 49) using the following validated mouse primers were obtained from Bio-Rad: Acta2 (dMmuCPE5117282) ...
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bioRxiv - Immunology 2023Quote: ... and secondary antibodies (Anti-Rabbit or Anti-Mouse, CST, 1:5000 and 1:2000) and revealed using Clarity Western ECL (BioRad). Image J was used to quantify the intensity of western blot bands ...
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bioRxiv - Immunology 2023Quote: ... and IFNγ) by the Bio-Plex Pro Mouse Cytokine Assay kit with the Bio-Plex 200 systems from Bio-Rad Laboratories ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: ... The viral genome concentration (including in mouse tissue) was determined by ddPCR in a QX200 Droplet Digital PCR System (BioRad), using Taqman primers/probe against the CMV enhancer (Sup ...
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bioRxiv - Microbiology 2023Quote: ... This step was followed immediately by an hour incubation with 1:5000 dilution of Goat α-mouse-HRP (Bio-Rad) in TBST ...
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bioRxiv - Biochemistry 2023Quote: ... and Mouse IgG HRP-linked whole Ab (Cytiva), then imaged using (ECL Select, Cytiva) with a ChemiDoc MP (Bio-Rad). For gel quantification ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation with primary antibodies (mouse anti-human Aβ 82E1 [1:500, IBL 27725], rat anti-CD68 [1:250, BioRad MCA1957] ...
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Effects of menopause and high fat diet on metabolic outcomes in a mouse model of Alzheimer’s diseasebioRxiv - Neuroscience 2023Quote: ... Diabetes-associated markers were assessed using the Bio-Plex Pro Mouse Diabetes 8-Plex Assay (171F7001M; Bio-Rad, Carlsbad, California) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... HRP-conjugated secondary antibodies used were anti-rabbit and anti-mouse at a dilution of 1:2000 and immunoblots were developed using Chemidoc imaging system (ChemiDoc, BioRad).
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A synthetic elastic protein as molecular prosthetic candidate to strengthen vascular wall elasticitybioRxiv - Cell Biology 2023Quote: ... The membrane was rinsed three times with TBS-T and then incubated with goat anti-mouse IgG coupled to horse radish peroxidase (1/10,000, 1706516, Bio-Rad) in TBS-T for 1 h at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... We utilized colorimetric detection to visualize the apoAI-EfpA band(s) on the membrane after WB transfer: The primary mouse antihistidine tag antibody (BioRad) and secondary goat antimouse IgG antibody conjugated to Alkaline Phosphatase (BioRad ...
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bioRxiv - Physiology 2024Quote: ... The secondary antibodies were goat anti-mouse HRP-linked and goat anti-rabbit HRP-linked (Bio-Rad, Hercules, CA, USA) for immunoblotting ...
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bioRxiv - Neuroscience 2024Quote: ... The blot was washed 3 times with TBST for 5 minutes and was incubated with 10 mL of 3% BSA/TBST (w/v) with 1 µL anti-mouse-HRP secondary antibody (1:10,000; 170-6516, Bio-Rad) for 30 minutes at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... the membrane was incubated with secondary antibody diluted 1:7500 in blocking buffer (anti-rabbit, anti-mouse HRP, Bio-Rad). Signal was detected using Pierce ECL Western Blotting Substrate (Fisher 32106) ...
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bioRxiv - Neuroscience 2024Quote: ... Brain sections were then incubated in primary antibodies at 4°C overnight (1:500, rat anti-mouse CD68, Bio-rad Cat ...
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bioRxiv - Immunology 2024Quote: ... Lysates were then cleared by centrifugation for 15 minutes at 14,000 rpm at 4°C and subjected to immunoprecipitation with anti-V5 mouse monoclonal antibody (#MCA1360, Bio-Rad) overnight at 4°C with rotation in DNA/RNA LoBind tubes ...
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bioRxiv - Microbiology 2020Quote: ... Lysates were analyzed by Luminex profiling (Luminex Corporation) using the Bio-Plex Pro Mouse Chemokine 31-plex panel (Bio-Rad; 12009159) at a 1:2 dilution following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2019Quote: ... and horseradish peroxidase (HRP)-conjugated goat anti-mouse immunoglobulin G (IgG) antibody (catalog no. 1721011, batch no. 64109318) were all purchased from Bio-Rad Laboratories (Hercules ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were incubated with StarBright Blue 700 Goat Anti-Mouse IgG secondary antibody (1:2500, Bio-Rad, Cat# 12004159, RRID: AB_2884948), StarBright Blue 520 Goat Anti-Rabbit IgG secondary antibody (1:2500 ...
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bioRxiv - Microbiology 2020Quote: ... Excess antibody was washed away with 3 x TBST rinses before the membrane was incubated with anti-mouse secondary antibody (Bio-rad) conjugated to horseradish peroxidase at a 1:15000 dilution in TBST at 25°C for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... for the rapamycin blot or with the fluorescent StarBright Blue 700 anti-mouse (1:5000) and StarBright Blue 700 anti-rabbit (1:5000) secondary antibodies (Bio-Rad) for the MRT68921 blots in TBS-T containing 5% powder milk ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes incubated with HRP (horseradish peroxidase)–conjugated antibodies (HRP rabbit, 111 035 144; HRP mouse, 115 035 146) were developed using Clarity western ECL substrate solutions (Bio-Rad) with a ChemiDoc XRS+ imaging system (Bio-Rad).
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bioRxiv - Immunology 2021Quote: ... Slides were incubated overnight at 4 °C with the following primary antibodies (1:100, in blocking buffer): mouse anti-human MBP (BMK-13, antihuman MBP (BMK-13, MCA5751 Bio-RAD), rabbit anti-human PD-L1 (E1L3N ...
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bioRxiv - Developmental Biology 2021Quote: ... horseradish peroxidase-conjugated goat anti-rabbit IgG (1706565, 1:500) and goat anti-mouse IgG (1706516, 1:500) from Bio-Rad. DAPI were purchased from Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... CD11b+ cells were used for flow cytometric analysis after cells were stained with Alexa647-conjugated rat anti-mouse CD204 antibody (clone 2F8; Bio-Rad Laboratories GmbH ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were washed thrice with TBS-T before incubation for one hour at room temperature with secondary antibodies (horse radish peroxidase goat anti-mouse or anti-rabbit; Bio-Rad), all at 1:5000 dilution ...
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bioRxiv - Molecular Biology 2020Quote: ... A gate was established for each factor using negative control samples stained with mouse IgG1 negative control antibodies (BIO-RAD, MCA928) or polyclonal rabbit IgG (R&D Systems ...
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bioRxiv - Physiology 2020Quote: ... Bradford protein assay kit as well as anti-mouse and anti-rabbit secondary antibodies were obtained from Bio-Rad (Hercules, CA). Antibodies against ERα (F-10 mouse – for WB) ...
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bioRxiv - Biochemistry 2021Quote: ... The membranes were then incubated with secondary antibodies (1:10000, goat anti-rabbit or goat anti-mouse IgG HRP conjugate (Bio-Rad) for 1 hour and detection was performed using the Immobilon-luminol reagent assay (EMP Millipore).
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bioRxiv - Microbiology 2020Quote: ... or anti-PfADF1 (1:2000, (Wong et al, 2011)) and HRP-coupled goat anti-mouse or -rabbit secondary antibody (1:5000, STAR120P/STAR121P, Bio-Rad). sBlots were washed in PBST and detected using ECL reagent (Amersham ...
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bioRxiv - Microbiology 2021Quote: ... and revelation with a IRDye 800CW Goat anti-mouse IgG secondary antibody (dilution 1/10000, Li-Cor Biosciences) using a ChemiDoc− Touch Imaging System (Bio-Rad).
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bioRxiv - Microbiology 2021Quote: ... qRT-PCR was carried out with the gene-specific mouse primers using SsoAdvanced SYBR Green supermix (Bio-Rad, Hercules, CA, USA) and CFX96 thermal cycler (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... the membranes were further incubated in secondary antibody solutions (TBST with 5% Non-Fat Dry Skinned Milk and Horseradish Peroxidase conjugated secondary antibodies Goat Anti-Rabbit/Mouse, 1:10000, Bio-Rad) for 2 hours at room temperature ...
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bioRxiv - Plant Biology 2021Quote: ... The blot was washed 3 times with TBST and incubated for 30 min in 1% milk prepared in TBST with anti-mouse HRP conjugate (Bio-Rad). Chemiluminescent detection was performed using SuperSignal West Dura Extended Duration (Thermo ...
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bioRxiv - Microbiology 2020Quote: ... Plates were washed thrice before the addition of horseradish peroxidase (HRP)-conjugated anti-mouse IgG (H+L) (Bio-rad, 170-6516) at 1:3,000 ...