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Citations for Bio-Rad :
901 - 950 of 2126 citations for Rabbit Anti Human IgG gamma chain Alexa Fluor 405 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... CLN5 transcripts were measured from the control and CLN5i neurons RNA by quantitative polymerase chain reaction[13] using an iTaq Universal Probes one-step kit (Bio-Rad, 1725141) on a Roche 96-well thermocycler ...
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bioRxiv - Cancer Biology 2019Quote: ... One μg of total RNA was used to perform reverse transcriptase–polymerase chain reaction (RT-PCR) using iScript supermix (Biorad, Hercules, CA). The sequence of PCR primers used are ...
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bioRxiv - Neuroscience 2020Quote: ... levels were quantified by real time polymerase chain reaction (RT-PCR) using SYBR® Green DNA intercalating dye and master mix (Bio-Rad) and the ABI 7900HT PCR machine ...
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bioRxiv - Biochemistry 2021Quote: ... For analysis of isolated RNA semi-quantitative reverse transcription–polymerase chain reaction (RT) PCR was performed with the iScript™ cDNA Synthesis Kit (BIORAD, Germany) according to the manufacturer’s instructions using 500ng of isolated RNA ...
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bioRxiv - Biochemistry 2020Quote: ... Real time polymerase chain reaction (RT-PCR) was performed with the CFX96 or CFX384 Touch(tm) Real-Time detection system (Bio-Rad Laboratories), using a SensiMix(tm ...
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bioRxiv - Neuroscience 2022Quote: ... Real-Time quantitative Polymerase Chain Reaction (RT-qPCR) was performed using a CFX96 Touch Real-Time PCR Detection System (Bio-Rad Laboratories) using 3 ng of cDNA per reaction and Sensifast SYBR No-ROX mix (Bioline Corporation ...
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bioRxiv - Neuroscience 2022Quote: ... and cDNA aliquots were analyzed in simultaneous real-time quantitative polymerase chain reactions using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, Canada) and a CFX96 Touch Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assays were performed using a C1000 TouchTM Thermal Cycler (Bio-Rad, Hercules, CA, USA). The reaction system included 5 μL SYBR® Premix Ex TaqTM (Takara ...
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bioRxiv - Immunology 2019Quote: ... One μg of total RNA was used to perform reverse transcriptase–polymerase chain reaction (RT-PCR) using iScript supermix (Biorad, Hercules, CA). The sequence of PCR primers used are ...
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bioRxiv - Microbiology 2021Quote: ... Fifty (50) μl polymerase chain reaction (PCR) mixtures were carried out in a T100™ Thermal Cycler (Bio-Rad Laboratories, Hercules, CA) using 100 ng of total DNA template along with AmpliTaq® DNA Polymerase reagents (Applied Biosystems ...
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bioRxiv - Cancer Biology 2022Quote: Droplet digital polymerase chain reaction (ddPCR) was performed using whole-slide FFPE extracted DNA on a QX200 Droplet Digital PCR System (BioRad, Hercules USA) following previously described protocols [20 ...
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bioRxiv - Bioengineering 2023Quote: The gene abundance of total bacteria and nitrifiers were determined by the quantitative polymerase chain reaction (qPCR) using the CFX real-time PCR detection system (Bio-Rad, USA). The primer sets chosen for AOB ...
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bioRxiv - Cell Biology 2024Quote: ... The cDNA obtained was analyzed by quantitative polymerase chain reaction (qPCR) using the CFX96 real-time PCR detection system (Bio-Rad Laboratories). The PCRs were performed in 20 µl reaction volumes that included 50 mM Tris–HCl (pH 8.6) ...
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bioRxiv - Microbiology 2024Quote: ... and human proinflammatory cytokines were quantified using the Bio-Plex Pro™ Human Inflammation Panel 1 (BioRad). Fluorescence was read using a MAGPIX System (Luminex ...
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bioRxiv - Immunology 2021Quote: ... Slides were incubated overnight at 4 °C with the following primary antibodies (1:100, in blocking buffer): mouse anti-human MBP (BMK-13, antihuman MBP (BMK-13, MCA5751 Bio-RAD), rabbit anti-human PD-L1 (E1L3N ...
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bioRxiv - Neuroscience 2019Quote: ... The membrane was incubated using appropriate HRP secondary antibodies (donkey anti-rabbit 711-035-152, donkey anti-mouse 715-035-150, Jackson Immunoresearch Laboratory and Biorad ChemiDoc XRS System) or fluorescent secondary antibodies (IRDye 800 CW ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were then incubated for 1 h with either anti-mouse or anti-rabbit secondary antibodies conjugated to horseradish peroxidase (Bio-Rad Laboratories, Hercules, CA). Bands were visualized and subjected to densitometric analysis using an Odyssey Fc system (LI-COR Biotechnology ...
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bioRxiv - Molecular Biology 2020Quote: ... and rat IgG and visualized using chemiluminescence (Biorad) and an ImageQuant LAS 4000 scanner (GE Healthcare) ...
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bioRxiv - Biochemistry 2022Quote: ... Mouse IgG antibody (PMP01X) was from Bio-Rad. Rabbit IgG antibody (P120-101 ...
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bioRxiv - Cell Biology 2023Quote: ... goat α-mouse IgG HRP (Bio-Rad, 1721011) or goat α-rabbit IgG HRP (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... or IgG (1 µg, Bio-Rad, PRABP01, RRID:AB_321631) as negative control ...
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bioRxiv - Cancer Biology 2023Quote: ... On-bead ELISA readouts were measured using the standard PE and APC detection filter parameters on a ZE5 flow cytometer (Bio-Rad; 405, 488, 561, and 640 lasers) in the Scripps Research Flow Cytometry Core Facility ...
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bioRxiv - Cell Biology 2020Quote: ... The secondary antibodies used for western blot and IF include: goat anti-rabbit HRP (1:10,000, BioRad), goat anti-mouse HRP (1:10,000 ...
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bioRxiv - Molecular Biology 2020Quote: ... resolved by SDS-PAGE and observed by immunoblot using rabbit anti-Ube2N (Bio-Rad, AHP974, 1:1000).
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bioRxiv - Pathology 2019Quote: ... the cells were incubated with anti- MNV VP1 rabbit polyclonal antibody labeled by Dylight 488 (LNK221D488, BioRad) for 30 min at 4°C ...
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bioRxiv - Bioengineering 2019Quote: ... Blots were incubated the following day with 0,33μg/ml Goat-anti-rabbit or -mouse HRP (Bio-Rad) in blocking buffer for 1h at room temperature ...
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bioRxiv - Biophysics 2020Quote: ... Primary antibodies were detected using Immun-Star horseradish peroxidase-conjugated goat anti-rabbit antibodies (Bio-Rad 1705046) and Western Lightning Plus ECL chemiluminescence reagents (Perkin Elmer NEL1034001 ...
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bioRxiv - Immunology 2023Quote: ... Detection was carried out with a goat anti-rabbit horse radish peroxidase (HRP) conjugated secondary antibody (BioRad), visualized with the enhanced chemiluminescent SuperSignal reagent (Pierce) ...
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bioRxiv - Microbiology 2023Quote: ... Secondary antibodies used are goat anti-rabbit HRP conjugated antibodies (dilution 1:10,000) (BioRad Cat. No. 1706515) and goat anti-rabbit HRP (BioRad Cat ...
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bioRxiv - Neuroscience 2023Quote: ... Bound antibody was detected by horseradish peroxidase-conjugated anti-rabbit secondary antibody (1/20000: 1705046, Bio-Rad) and developed using Clarity™ Western ECL Substrate (1705060 ...
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bioRxiv - Physiology 2019Quote: ... Real time polymerase chain reaction (RT-PCR) was performed with the CFX96 or CFX384 Touch™ Real-Time detection system (Bio-Rad Laboratories), using a SensiMix™ (BioLine ...
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bioRxiv - Physiology 2019Quote: ... Transcript levels were analyzed by real-time polymerase chain reaction (RT-PCR) (fluorescence detection of SYBR green) in an iCycler iQ apparatus (Bio-Rad, Hercules, CA), with normalization against 18S as an endogenous control ...
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bioRxiv - Plant Biology 2020Quote: ... and the quantitative real-time polymerase chain reaction (qRT-PCR) was performed using the iScript™ One-Step RT-PCR Kit (Bio-Rad®), marking with SYBR® Green (Bio-Rad®) ...
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bioRxiv - Cancer Biology 2020Quote: ... Quantitative polymerase chain reaction (qPCR) was performed with specific primers (Supplementary Table 2) using the iTaq™ Universal SYBR® Green Supermix (Bio-Rad) and CFX ConnectTM Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Immunology 2020Quote: SARS-COV-2 RBD-SD1 gBlock (IDT) was amplified by polymerase chain reaction (PCR) with iProof High-Fidelity PCR system (Bio-Rad, Cat # 1725310) following the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2021Quote: ... Reverse Transcription – Quantitative Polymerase Chain Reaction (RT-qPCR) reactions were performed using SsoAdvanced™ universal SYBR® green (Bio-Rad, Hercules, CA, USA) and run on the CFX Connect (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... Quantitative real time polymerase chain reaction for all targets was performed on the CFX-96 Real Time System using iQ SYBR Green Supermix (Bio-Rad, Hercules, CA). Products were confirmed by gel electrophoresis ...
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bioRxiv - Neuroscience 2021Quote: ... was used from each sample for One-step reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) using the iTaq™ Universal SYBR® Green One-Step kit (Bio-Rad) in the StepOnePlus™ Real Time PCR system (Applied Biosystems) ...
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bioRxiv - Genetics 2022Quote: ... Quantitative RT-polymerase chain reaction (qRT-PCR) experiments were performed using the CFX96 Touch Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules) with PowerUP SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Genetics 2022Quote: ... Quantitative RT-polymerase chain reaction (qRT-PCR) experiments were performed using the CFX96 Touch Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules) with PowerUP SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Physiology 2022Quote: For the separation of myosin heavy chain isoforms a large format SDS-PAGE setup with integrated cooling core was used (Protean II xi, Bio-Rad Laboratories, Inc.). A 5% stacking gel ...
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bioRxiv - Physiology 2022Quote: ... Primers flanking the target site were used to amplify the target region of the gene in a 10 µL polymerase chain reaction (PCR) containing: 5 µL 2 × SuperMix (Bio-Rad, Hercules, CA), 1 µL genomic DNA ...
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bioRxiv - Molecular Biology 2023Quote: Relative genetic expression was determined using Real Time Quantitative Polymerase Chain Reaction (RT-qPCR) (CFX96™ Real-Time System, Bio-Rad, CA, USA) using commercially available TaqMan primers (Tables 1-3 ...
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bioRxiv - Immunology 2023Quote: ... Viral RNA genome was detected and quantified by reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) on a BioRad real-time CFX96 detection system (BioRad, Hercules, United States). The target sequence for amplification was the viral RNA-dependent RNA polymerase [26].
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Quantitative real time polymerase chain reaction (qPCR) was performed using the iQ™ SYBR® Green Supermix (170888, Bio-Rad, Hercules, CA, USA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... We optimized the recommended polymerase chain reactions (PCR) cycling conditions and performed a touchdown PCR in a T100 thermocycler (Bio-Rad, Hercules, CA). The total reaction volume was 50 µl which contained 2 µl of genomic DNA at 50 ± 25 ng/µl ...
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bioRxiv - Neuroscience 2021Quote: Autaptic murine striatal neurons (DIV 15-18) were incubated with 1 μg/ml human GABAAR mAb or control antibody (anti-CD52, Bio-Rad, #HCA175) at 37°C for 24 hours ...
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bioRxiv - Biochemistry 2022Quote: ... rabbit (BIO-RAD, product number ...
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bioRxiv - Immunology 2023Quote: ... Rabbit complement (BioRad) was added and incubated for 60 min ...
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bioRxiv - Genomics 2020Quote: ... Secondary antibody used were an HRP-conjugated goat anti-mouse or rabbit at 1:25,000 (BioRad, Hercules, CA).