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Citations for Bio-Rad :
901 - 950 of 1282 citations for Oxychlordane Unlabeled 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Samples were then centrifuged (2500 × g for 5 min) and pellets were resuspended in 100 μL of PAGE Laemmli Sample Buffer (Bio-Rad, 1610747) mixed with 50 μL TE 1X pH 8.0 ...
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bioRxiv - Bioengineering 2022Quote: ... pH 8.3 at 100 V for 2 h at 2-8 °C using PowerPac Basic setup (Bio-Rad Laboratories Inc., Hercules, CA). Post-electrophoresis ...
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bioRxiv - Bioengineering 2022Quote: ... the cells were blocked with Bovine Serum Albumin (BSA) in DPBS for 30 min and then incubated with anti-His6 antibody (1:100, Bio-Rad, MCA1396) for 2 h at 25°C ...
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bioRxiv - Cell Biology 2022Quote: ... for 20 minutes treated with PBS containing 1 % w/v BSA and 0.1 % v/v Triton X-100 (Bio-Rad, München, Germany) for 30 minutes ...
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bioRxiv - Microbiology 2022Quote: ... 100 ng plasmid DNA and 100 μL of electrocompetent mycobacteria were mixed and transferred to a 2 mm electroporation cuvette (Bio-Rad #1652082). Where necessary ...
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bioRxiv - Genetics 2023Quote: ... final concentration 100 ng of cDNA was used for expression analysis with iTaq Universal SYBR Green Supermix (Bio-Rad, Cat. No. 1725121). qPCR was performed in a CFX96 Bio Rad reader with following cycles ...
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bioRxiv - Cell Biology 2023Quote: 5 μg DNA/106 cells or 100 nM siRNA /106 cells were transfected by electroporation (200 mV, 950 μF and 480 Ω; Bio-Rad). Expression was measured 24-48 h post-transfection ...
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bioRxiv - Cell Biology 2023Quote: ... RNA was extracted using TRIzol and 100 ng of RNA were reverse transcribed using Quantitect followed by qPCR using iTaq SYBRgreen (Bio-rad, 1725122).
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bioRxiv - Cancer Biology 2023Quote: ... were loaded on 10 % or 4–20 % Mini-PROTEANR TGX™ Precast Protein Gels and separated at 100 V in Tris/Glycine/SDS buffer in Mini-Protean Tetra System (Bio-Rad). The proteins were transferred to a polyvinylidene difluoride membrane (PVDF ...
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bioRxiv - Evolutionary Biology 2023Quote: Protein was separated by electrophoresis on 12% SDSCPAGE and transferred to a PVDF (polyvinylidene difluoride) membrane at 100 mA for 2 h using a Mini-PROTEAN Tetra system (Bio-Rad, USA). For detection of PpSerpin1 isoform proteins or PrPAP1 ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 μg of cell lysates were separated on a 4-20% resolving gel (Bio-Rad Mini-PROTEAN TGX Precast Gel #456-1094), transferred to a PVDF membrane for analysis using anti-FLAG (Sigma Aldrich #F3165 ...
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bioRxiv - Zoology 2024Quote: ... and 100 ng of RNA were used for cDNA synthesis with the iScriptTM gDNA Clear cDNA Synthesis Kit (Bio-Rad Laboratories, USA), resulting in a final concentration of 5 ng/μl for each sample.
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bioRxiv - Pharmacology and Toxicology 2024Quote: A total protein amount of 100 μg for each sample was separated on 12% Criterion gels (Bio-Rad Laboratories, Hercules, Ca, USA), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... 20 µL reaction mixes containing 100 ng template RNA were added to a 96-well PCR plate (Bio-Rad Cat. No. MLL9601) and sealed with adhesive Microseal ‘B’ PCR Plate Sealing Film (Bio-Rad Cat ...
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bioRxiv - Neuroscience 2024Quote: ... proteins were eluted from sepharose beads by boiling at 95 - 100 °C for 5 min with 40 µl sample buffer diluted from 4x sample buffer (Bio-Rad, 1610747) with Lysis Buffer ...
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bioRxiv - Microbiology 2023Quote: ... 100 µL of the electrocompetent cells were aliquoted into pre-chilled 0.2 cm gap Gene Pulser Electroporation Cuvettes (Bio-rad Laboratories #1652086) and kept on ice ...
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bioRxiv - Genetics 2024Quote: ... at 75 V for 15 min and 100 V for 1 hr 15 min using 1X Tris/Glycine/SDS running buffer (BioRad Cat. # 1610772). Following electrophoresis ...
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bioRxiv - Molecular Biology 2024Quote: ... The remaining 30 μL sample was run on a 10% acrylamide SDS-PAGE gel at 100 V for 15 minutes and stained with Coomassie (Bio-Rad #P1610786) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... The eluted protein was subjected to an additional purification step using fast protein liquid chromatography (FPLC) and a Superdex 200 Increase 10/300 GL column (Cytiva, 28990944) in Chelex 100-treated (Bio-Rad, 1422832) dialysis buffer (25 mM HEPES pH 7.4 ...
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bioRxiv - Molecular Biology 2020Quote: ... the total protein concentration was adjusted to 1.8 mg/ml using Bradford reagent (Bio-Rad) and 1 ml aliquots were separated over a Superdex 200 16/60 equilibrated with 20 mM Tris-HCl pH 7.5 and 50 mM NaCl ...
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bioRxiv - Biochemistry 2019Quote: ... with or without CLEC-2 antibody (3 μg/ml final concentration; Bio-Rad, Oxford, UK). The plate was incubated in the dark for the indicated time and the reaction was stopped by addition of 200 μl 1% ice-cold formalin ...
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bioRxiv - Molecular Biology 2019Quote: ... at a 1 ml flow rate using a NGC Quest 10 chromatography system (Bio-Rad). Buffer A was 5% acetonitrile 0.01M ammonium bicarbonate ...
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bioRxiv - Synthetic Biology 2019Quote: ... The nickel sepharose-lysate suspension was poured onto a 10 mL disposable column (Bio-Rad), and the settled resin washed with 20 column volumes of a first wash solution (50 mM KH2PO4 ...
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bioRxiv - Biochemistry 2020Quote: ... The collected beads were poured into a 25 mL Econo-Column Chromatography Column (Bio-Rad) and washed with four column volumes of wash buffer (20 mM Tris–HCl pH 8.0 ...
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bioRxiv - Cell Biology 2021Quote: ... The last wash was conducted in a disposable 10 ml Poly-prep column (Bio-RAD) and the protein was eluted from the beads with elution buffer (25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... Mass calibration was done by using 2 mg/ml BSA standard (Bio-Rad, cat.5000206). PBS pH 7.4 was used as isocratic buffer ...
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bioRxiv - Biophysics 2022Quote: ... Detergent was removed from the reaction by addition of 0.1 g/mL Biobeads (Bio-Rad) and incubation at 4 °C for 1 h ...
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bioRxiv - Biochemistry 2020Quote: ... The 3 mg/mL protein sample was injected onto NGC Liquid Chromotography System (Bio-Rad) equipped with a HiPrep Sephacryl S-500 HR column equilibrated in FPLC buffer and separated into 156 ...
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bioRxiv - Microbiology 2021Quote: ... Detergent was removed by incubation with 0.6 g/ml Bio-Beads SM-2 (Bio-Rad) at 4 °C for 2 hours ...
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Gatekeeper helix activates Golgi SM protein Sly1 and directly mediates close-range vesicle tetheringbioRxiv - Cell Biology 2020Quote: ... Sly1-bound resin was collected in a 25 mL disposable Econo-Pac column (Bio-Rad) by gravity and washed with 25 mL of SLY1 buffer supplemented with 50 mM imidazole at pH 7 ...
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bioRxiv - Synthetic Biology 2020Quote: ... The slurry was applied to a 10-mL Poly-Prep chromatography column (Bio-Rad, USA), which was then washed with 10 mL of purification buffer B (purification buffer A with 50 mM imidazole) ...
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bioRxiv - Biochemistry 2020Quote: ... the beads was transferred to a gravity-flow Econo column (49 ml volume; Bio-Rad) and washed with 2 column volumes (CV ...
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bioRxiv - Bioengineering 2019Quote: ... generated droplets or Gelbeads were extracted into PCR tubes (0.2 mL individual PCR tubes, BioRad) and incubated in a thermal cycler (T100 ...
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bioRxiv - Biophysics 2022Quote: ... lysate-resin suspension was loaded onto a 20 ml polypropylene gravity-flow column (Bio-Rad). After collecting the flow through ...
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bioRxiv - Biophysics 2022Quote: ... lysate-resin suspension was loaded onto a 20 ml polypropylene gravity-flow column (Bio-Rad). After collecting the flow through ...
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bioRxiv - Biochemistry 2022Quote: ... Detergents were removed by the sequential addition of 60 mg/mL Biobeads SM2 (Bio-Rad) for three times within 3 h ...
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bioRxiv - Microbiology 2022Quote: ... SasG-His6 was then purified using a pre-packed 5 ml IMAC cartridge (Bio-rad) on a Bio-rad FPLC ...
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bioRxiv - Microbiology 2022Quote: ... Membrane pellets were resuspended at 11 mg/ml (as determined by Bradford assay, Bio-Rad) in 20 mM Tris-HCl pH 7.4 ...
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bioRxiv - Microbiology 2022Quote: ... Detergents were removed by incubation with 0.8 mg ml-1 Bio-Beads SM2 (Bio-Rad) overnight at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... Detergents were removed by incubation with 0.8 mg ml-1 Bio-Beads SM2 (Bio-Rad) overnight at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... then incubated with PBST containing 1% BSA and 22.5 mg/mL glycine (Bio-Rad 1610718) for 30 minutes at 23°C ...
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bioRxiv - Cell Biology 2023Quote: ... and cell density (cells/ml) was measured by an automated cell counter (TC20, Bio-Rad). The culture was diluted by medium lacking nitrogen and carbon sources to a density of 1×106 cells/ml ...
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bioRxiv - Biophysics 2022Quote: ... UCP1 was detected using a mouse anti-pentahistidine tag:HRP 0.2 μL/mL (MCA5995P – Bio-RAD). VDAC1 Porin was used as a mitochondrial loading control and detected by a mouse anti-VDAC1 0.1 μL/mL (16G9E6BC4 - Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... Quantification of cell number per ml was measured on a TC20 Cell Counter (Bio-Rad).
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bioRxiv - Cell Biology 2023Quote: ... qPCR reactions were prepared using 5 ml SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, USA) and 4 ml 1:6 diluted cDNA template along with 200 nM forward and reverse primers in a total volume of 10 ml per reaction ...
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bioRxiv - Synthetic Biology 2023Quote: ... with 0.2 ml Flat PCR Tube 8-Cap Strips (Bio-Rad™, optical, ultraclear, #TCS0803). Optimum amplification conditions were determined for each by amplifying six concentrations of a 5× dilution series of MG1655 pJK14-Tn4rev pZA31-mCherry-tnpB using two-step amplification with a thermal gradient of 55–75°C ...
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bioRxiv - Physiology 2023Quote: ... Matching slides per animal were immunofluorescence (IF) stained for cd68 (5 µg/mL, MCA1957, BioRad) followed by donkey anti-rat CF750 (2 µg/mL ...
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bioRxiv - Microbiology 2023Quote: ... Protein-bound resin was then applied to a 10-mL chromatography column (BioRad, Hercules, CA) and washed twice with 10 mL wash buffer (50 mM NaH2PO4 ...
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bioRxiv - Biochemistry 2023Quote: ... before rinsing quickly with 2x10 mL TBS before treating with clarity ECL substrate (Bio-Rad). Signals were detected using a Bio-Rad ChemiDoc Imaging System ...
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bioRxiv - Biochemistry 2023Quote: ... To a solution of Affi-Gel 10 (Bio-Rad, 2 ml, 0.03 mmol, 1.0 eq) in a solid phase synthesis tube with frit was added a solution of compound 11 (27.7 mg ...