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Citations for Bio-Rad :
901 - 950 of 2599 citations for 5 2 Chloronicotinoyl 2 furoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... The pellets were resuspended with 2 % SDS and loaded onto a cellulose acetate membrane assembled in a slot blot apparatus (Bio-Rad). The membrane was washed with 0.2% SDS and retained SDS-insoluble mHTT aggregates were detected with mouse anti-polyQ antibody (Millipore ...
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bioRxiv - Plant Biology 2023Quote: ... tabacum) BY-2 suspension-cultured cells and biolistic bombardment using a PDS-1000/He biolistic particle delivery system (Bio-Rad Laboratories) were performed as previously described (Banjoko and Trelease ...
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bioRxiv - Cell Biology 2023Quote: ... Protein content was confirmed by mixing 2 μL of each elution step with 200 μL Bradford solution (Protein assay dye reagent (Bio-Rad) diluted 1:5 in H2O) ...
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bioRxiv - Genomics 2023Quote: ... 10ul of PCR product of each replicate for each gene was loaded in separate wells in 2% agarose gels and imaged using a ChemiDocTM MP Imaging system (BioRad, USA). All replicates for each gene were loaded on the same gel ...
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bioRxiv - Biochemistry 2022Quote: An equivalent volume of ∼150 µg FBS was first diluted 1:2 with loading buffer (consisting of 2X Laemmli Sample Buffer (#1610737, Bio-Rad) plus a 5% (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... 2 μg of RNA template was converted to cDNA using iScript™ cDNA Synthesis Kit (1708891, Bio-Rad, Hercules, CA, USA), according to the manufactures’ instructions ...
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bioRxiv - Microbiology 2023Quote: ... The reaction mixtures contained 100 ng RNA template per sample diluted 2-fold with qPCR solution (iTaq Universal SYBR Green One-Step Kit, Bio-Rad) containing the primers D2S and D2C ...
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bioRxiv - Genetics 2024Quote: ... Membranes were were in contact with the luminol-H2O2 solution for 2 minutes and then exposed for imaging using the Chemidoc imaging system (Bio-Rad).
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bioRxiv - Cell Biology 2024Quote: ... clarified at 20,000 g for 2 minutes and 15 µL was loaded onto a 4-20% tris-glycine SDS-PAGE gel (Biorad #5671094). Proteins were then transferred to nitrocellulose (Sigma #10600001 ...
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bioRxiv - Biophysics 2024Quote: ... then diluted below the CMC of C12E8 with reconstitution buffer and added to 0.5 g methanol-activated Biobeads SM-2 (Bio-Rad; 1523920). The mixture was rotated end over end for 18 hours and then subject to room temperature size exclusion chromatography on a Superose 6 10/300 GL column ...
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bioRxiv - Microbiology 2024Quote: ... 10 µg Capped mRNA transcripts were electroporated into BHK-21 cells in a 0.2 cm cuvette (25 μF, 1.5 kV, and 200 Ω) with the help of Gene Pulser Xcell Electroporation system (Bio-Rad) and resuspended in antibiotic-free DMEM supplemented with 10% FBS ...
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bioRxiv - Plant Biology 2024Quote: ... RT-qPCR was performed using 2 uL of diluted cDNA in a 20-µL reaction with SYBR Green dye polymerase mix (SsoAdvanced; Bio-Rad) in white-walled plates in a CFX96 thermocycler (Bio-Rad) ...
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bioRxiv - Plant Biology 2024Quote: ... 500 μL of protoplasts in a total volume of 600 μL electrotransfection buffer were electrotransfected with 2-6 μg plasmid DNA using the Gene Pulser XcellTM (Bio-Rad) with a pulse at 160 V for 10 ms ...
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bioRxiv - Immunology 2024Quote: ... for two hours at RT with mild rotation and proteins in FhNEJ-Som were acetone-precipitated using the ReadyPrep 2-D Cleanup kit (BioRad, #1632130) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... detergents in FhNEJ-Teg and FhNEJ-Som were removed as previously described 46: FhNEJ-Teg was incubated with SM-2 Adsorbent Bio-Beads (BioRad, #1523920) for two hours at RT with mild rotation and proteins in FhNEJ-Som were acetone-precipitated using the ReadyPrep 2-D Cleanup kit (BioRad ...
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bioRxiv - Immunology 2024Quote: ... membranes were repeatedly washed with TBST and incubated for 2 h with the appropriate HRP-conjugated secondary mouse antibody (1/1500, BioRad Laboratories) or secondary goat anti□rabbit IgG HRP (1/5,000 Cusabio Cedarlane ...
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bioRxiv - Neuroscience 2024Quote: ... Viability of magnetically-captured cells (eluted into a new tube away from the magnet) was assessed by trypan blue staining (1:2 dilution sample in trypan blue; BioRad 1450013). Cell viability was quantified as the proportion of cells that did not take up the dye ...
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bioRxiv - Physiology 2024Quote: ... The cells were centrifuged at 1500 rpm for 10 mins at 4°C and the pellet was resuspended in 5 ml single-cell suspension buffer (2 mM EDTA, 0.5 BSA, 1% FBS in DPBS) and the cells were counted (Bio-Rad TC20) to prepare 1×106 aliquots.
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bioRxiv - Microbiology 2024Quote: ... Samples were mixed in nanodisc buffer (25 mM Napi, 100 mM NaCl, pH 7.0) with Bio-beads™ SM-2 Adsorbent Media (Bio-RAD) for cholate and detergent (DDM ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were washed in PBS-T and developed for 2 min with ClarityTM Western ECL Substrate (Bio-Rad, CA, United States). Imaging was performed using a ChemiDocTM MP Imaging System with Image LabTM software (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA was synthesized starting from 1-2 μg of total RNA using the iScript cDNA Synthesis Kit (Bio-Rad, catalog #1708891). RT-qPCRs were carried out with the Luna® Universal qPCR Master Mix (New England Biolabs ...
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bioRxiv - Biophysics 2024Quote: ... This solution was diluted 4-fold then placed in 3 mL Slide-a-lyzer 10 kDa MWCO cassette (Pierce) and dialyzed against 250 mL of SUV buffer in the presence of 2 g SM2-biobeads (Bio-Rad) overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... and 70 mM 2-mercaptoethanol) and set on the glucose oxidase enzymatic system (GLOX) for STORM using a frame-sealed incubation chamber (Bio-Rad). 2D-STORM image acquisitions were performed using a Nikon N-STORM with Eclipse Ti-E inverted microscope and laser TIRF illuminator (Nikon Instruments Inc.) ...
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bioRxiv - Cancer Biology 2024Quote: ... Relative mRNA expression was quantified using the 2-ΔΔCT method [95] after obtaining CT values from the CFX Manager software (Bio-Rad). Primer sequences are provided in Supplementary Table 6.
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bioRxiv - Biochemistry 2024Quote: ... The gels were dried at 56 °C for 2 hours in a BioRad Model 583 gel dryer (BioRad Cat # 165-1745), rehydrated and subjected to southern blotting using 5’-32P labeled oligonucleotide probes targeting either the extrusion containing strand (Fwd 1947 ...
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bioRxiv - Bioengineering 2024Quote: ... qPCR was performed with TaqMan Fast Advance Mastermix and primer pairs listed in Supplementary Table 2 and analysed using a CFX96TM Real-Time PCR System (Bio-RAD). Gene expression was normalised to glyceraldehyde 3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Biochemistry 2024Quote: ... Total RNA (2 μg) was subjected to cDNA synthesis using the iScript cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA, USA). Real-time PCR was performed on an Applied Biosystems 7300 Sequence Detection System (Applied Biosystems ...
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bioRxiv - Cell Biology 2024Quote: ... transferred to a nitrocellulose membrane (Cytiva) for 2 h (at 30V) and detected using infrared light on a Chemidoc MP (Bio-Rad).
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bioRxiv - Genetics 2024Quote: ... 2 µL of the diluted cDNA samples are supplemented with 10 µL of Supermix 2X ddPCR (without dUTP, Bio-Rad, #1863024), 1 μl of target probe (ZEN™ FAM)/primers mix (final concentration of 750 nM of each primer and 250 nM of probe ...
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bioRxiv - Cell Biology 2024Quote: ... The cDNA was amplified using the primers listed in Extended Table 2 and the SsoAdvanced universal SYBR Green supermix (Bio-Rad). Amplimers for vacA ...
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bioRxiv - Biophysics 2024Quote: ... 5% acrylamide (BIORAD), 0.1% SDS ...
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bioRxiv - Molecular Biology 2021Quote: Amplification was carried out using PCR mixture-2 blue (AmpliSens, Russia) containing Taq-polymerase on a T100 Thermal Cycler (Bio-Rad, USA). Next ...
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bioRxiv - Molecular Biology 2021Quote: ... and 2% β-mercaptoethanol) and separated on a 10% gel by SDS-PAGE (TGX Stain-Free™ FastCast™ Acrylamide Solutions; BIORAD) or 4-15% gel (Mini-PROTEAN® TGX Stain-Free™ ...
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bioRxiv - Genomics 2022Quote: ... Each sample was tested by PCR systems (threshold – 0,050) to the presence of SARS-CoV-2 using a CFX Connect Real-Time PCR System (BioRad, Hercules, California, USA). RT-PCR were implemented in 40 μl of final volumes ...
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bioRxiv - Biochemistry 2021Quote: ... the reactant was mixed with the 2 × SDS-PAGE sample buffer containing 100 mM β-mercaptoethanol and run on 12% Mini-PROTEAN TGX Precast Gel (Bio-Rad) without a boiling step ...
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bioRxiv - Microbiology 2021Quote: ... The amplified genomic DNA from the PPA strains was resolved using 2% agarose gel and visualized in the Gel DocTM XR+ documentation system (Bio-Rad, USA).
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bioRxiv - Microbiology 2021Quote: ... aeruginosa isolates were separated in 2% agarose gel and visualized their banding patterns in the Gel DocTM XR+ documentation system (Bio-Rad, USA).
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bioRxiv - Neuroscience 2021Quote: ... Samples were then loaded onto 4-15% Criterion™ TGX™ Precast Midi Protein Gels (12 + 2 wells, 45 μl) (Bio-Rad) and subjected to SDS-PAGE at 200 V for ~45 min in 10× Tris/Glycine/SDS running buffer (Bio-Rad) ...
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bioRxiv - Neuroscience 2021Quote: ... Master mixes containing primer pairs against FIBCD1 or B2M (Beta-2-microglobulin, housekeeping gene) were prepared with iTaq Universal SYBR Green Supermix (Bio-Rad #1725122). The mastermixes were dispensed into each well containing cDNA and incubated on ice for 15 minutes to allow the cDNA to dissolve ...
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bioRxiv - Plant Biology 2020Quote: ... Reactions were performed with 2 × Taq Master Mix (Vazyme, Nanjing, China) on a Bio-Rad Thermal Cycler (Bio-Rad, Hercules, CA, USA) using the following procedure ...
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bioRxiv - Genomics 2021Quote: ... PCR products were resolved using a 2% agarose tris-acetate-EDTA gel and a 177 bp band was visualized using the ChemiDoc™ imaging system (Bio-Rad).
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bioRxiv - Microbiology 2021Quote: ... 100 ng plasmid DNA and 100 μL of electrocompetent mycobacteria were mixed and transferred to a 2 mm electroporation cuvette (Bio-Rad #1652082). Where necessary ...
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bioRxiv - Neuroscience 2020Quote: ... and followed by incubation with HRP-conjugated secondary antibodies for 2 hours at room temperature: goat anti-rabbit IgG (BioRad, #170-6515) or goat antimouse IgG (BioRad ...
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bioRxiv - Plant Biology 2020Quote: ... or 1 μl (for the rest) of a 1/2 dilution of the cDNA was added to a reaction containing 1X of SsoFast EvaGreen (Bio-Rad, USA), 0.5 μM Forward and Reverse primers (Table 2) ...
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bioRxiv - Microbiology 2020Quote: ... which subsequently performed 2-DE using a 7 cm immobilized pH gradient (IPG) strips (pH range of 3—10, Ready strip, Bio-Rad, USA).
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bioRxiv - Cancer Biology 2021Quote: ... 60 of High Capacity NeutrAvidin slurry (Thermo PI29204) were washed three times with 1 mL 2X RIPA/1mM EDTA buffer in 2-mL chromatography columns (Bio-Rad 7326008) attached to a vacuum manifold (Promega A7231) ...
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bioRxiv - Cell Biology 2021Quote: ... Selected siRNAs were spotted onto a gridded MatTek dish (P35G-2-14-C-GRID) using a contact spotter (ChipWriter Pro-Bio-Rad Laboratories) resulting in a layout of 4 × 8 spots 40 ...
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bioRxiv - Microbiology 2022Quote: SARS-CoV-2 viral RNA loads were determined using Droplet Digital PCR (ddPCR) supermix for probes without dUTP (Bio-Rad Laboratories Ltd) and the QX200 Droplet Digital PCR System Workflow (Bio-Rad Laboratories Ltd) ...
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bioRxiv - Neuroscience 2020Quote: Brain tissues were homogenized in lysis buffer and then combined with 2 x Laemelli buffer (Bio-Rad Laboratories, Inc., Hercules, CA, USA). Protein samples were then separated on 7.5 to 12.5% v/v sodium dodecyl sulfate (SDS ...
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bioRxiv - Developmental Biology 2021Quote: ... following by 25 cycles of preamplification using mix of 47 pairs for MTE primers, each at final 50nM concentration (Supplementary information, Supplementary Table 2) and SsoAdvanced™ PreAmp Supermix (Cat# 1725160, Bio-Rad). After amplification ...