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Citations for Bio-Rad :
9001 - 9050 of 9625 citations for Recombinant Human EGFR protein Fc tagged R PE labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... and light units were normalized by the total protein concentrations of the corresponding WCEs determined using the Bradford reagent (BioRad, 5000006).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... before being loaded on a SDS-PAGE gel (4–20% Criterion™ TGX Stain-Free™ Protein Gel, Bio-Rad #5678095). Western blot was then performed as described in the previous section.
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bioRxiv - Plant Biology 2023Quote: ... The proteins were then transferred to polyvinylidene difluoride membranes using NuPAGE Transfer Buffer using a Trans-Blot Turbo Transfer System (Bio-Rad) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were denatured by boiling for 10 minutes in the DTT containing sample buffer and separated by 4-20% Mini-PROTEIN TGX gels (BIO-RAD) and then transferred to the 0.2 μm PVDF membrane (BIO-RAD) ...
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bioRxiv - Biochemistry 2023Quote: ... Cyt-b5 and other protein band intensities were determined using the volume contour tool Image Lab (Bio-Rad; Hercules, CA, USA) or ImageJ software ...
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bioRxiv - Cell Biology 2023Quote: ... Equal amounts of total protein samples (40-50 µg) were resolved by polyacrylamide gels using self-made or pre-cast gels (Bio-Rad). Proteins were electroblotted on nitrocellulose membranes (Cytiva ...
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bioRxiv - Physiology 2023Quote: ... The subsequent protein pellet was solubilized in 50 µL Laemmli sample buffer and 20 µg protein was run in a 12.5% SDS-Page gel (BioRad Criterion system). The gels were fixed and stained with Coomassie blue (GelCode blue ...
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bioRxiv - Neuroscience 2023Quote: ... 10 µg of proteins in Laemmli buffer and protein standard were loaded on 4%-15% Criterion TGX Stain-Free protein gel in running buffer TGS 1XL(all from Bio-Rad) and transferred to a 0.2Lµm PVDF membrane with the Trans-Blot Turbo RTA Midi Transfer Kit (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: ... proteins (20 μg per lane) were separated by SDS–polyacrylamide gel electrophoresis and transferred to a polyvinylidene difluoride membrane (Bio-Rad). Membranes were blocked in 4% milk in TBST [0.01 M tris (pH7.4) ...
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bioRxiv - Cell Biology 2023Quote: All the protein samples were separated by sodium dodecyl sulfate polyacrylamide (SDS)-PAGE and were transferred to nitrocellulose membrane (Bio-Rad laboratories ...
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bioRxiv - Cell Biology 2023Quote: ... Aliquots of cell lysates (40-60 μg of protein) were subjected to SDS/PAGE on an 8 to 15% (vol/vol) acrylamide gel (MiniProtean; Bio-Rad) including PageRuler Prestained Protein Ladder (Thermo) ...
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bioRxiv - Immunology 2023Quote: ... Even amounts of each protein sample were separated on a 8–10% polyacrylamide gel using Mini-PROTEAN Tetra Cell electrophoresis system (Bio-Rad). Upon completion of electrophoresis ...
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bioRxiv - Cell Biology 2023Quote: ... Band intensities were normalized to total protein in the sample quantified within each lane using Stain-Free Dye Imaging (Bio-Rad).
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bioRxiv - Bioengineering 2023Quote: Purified protein samples were visualized to evaluate purity by SDS-PAGE using the Bio-Rad mini-protean system (Bio-Rad laboratories). Samples were mixed in a 1:1 ratio with 2x Laemmli sample buffer containing 50 mM 1,4-dithiothreitol (DTT ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated by SDS-PAGE and transferred onto PVDF membranes applying the Trans-Blot Turbo Transfer system (Bio-Rad Laboratories). Membranes were blocked with 5% BSA or 5% NFDM in 1X TBS-T buffer and incubated with specific primary and secondary antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysate samples with the same amount of total protein (40-50 μg) were mixed with 4x Laemmli Sample Buffer (Bio-Rad #161-0737 ...
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bioRxiv - Cancer Biology 2023Quote: ... Sodium-dodecyl-sulfate polyacrylamide gel electrophoresis and transfers of proteins to .45uM nitrocellulose membranes were performed using the Protean 2 or 3 systems (Bio-Rad) and protocols provided by the manufacturer ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated by isoelectric focusing on immobilized linear pH gradient strips (pH 5 to 8, Bio-Rad, Hercules, CA, USA) before separation by SDS-PAGE ...
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bioRxiv - Molecular Biology 2023Quote: 10-20% of the samples containing immunoprecipitated ECTs for mass-spectrometry were subjected to SDS-PAGE in 4-20% CriterionTM TGXTM Precast Midi Protein gels (Bio-Rad) for visual assessment ...
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bioRxiv - Immunology 2023Quote: ... 8 µg protein per condition were separated by SDS-PAGE and blotted onto a 0.2 µm nitrocellulose membrane (Bio-Rad, #1704158) using a Trans-Blot® Turbo™ Transfer System (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: Proteins were separated by SDS-PAGE on 4-15% gradient Mini-Protean TGX Stain-free gels (cat. no. 4568083, Bio-Rad), transferred onto PVDF membranes (cat ...
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bioRxiv - Microbiology 2023Quote: Total protein extracts (5x106 cells) were separated by SDS-PAGE (10%) and immunoblotted on TransBlot Turbo Midi-size PVDF Membranes (Bio-Rad). Immunodetection was performed using the primary antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... 20 µg of the protein lysate were mixed with 7.5 µl of 4X Laemmli sample buffer (Bio-Rad Laboratories, Cat #: 1610747) containing 10% β-mercaptoethanol (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... The lysates were centrifuged at 18,000 x g for 30 minutes at 4 °C and the supernatant was incubated with SurebeadsTM Protein A Magnetic Bead (Bio-rad) conjugated with rabbit monoclonal DYKDDDDK antibody at RT for two hours ...
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bioRxiv - Pathology 2023Quote: ... total proteins were extracted and examined as previously described.59 The blotting was performed on the PVDF membrane (Bio-Rad, USA) and imaged with the Western Blotting Detection Kit (Advansta ...
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bioRxiv - Microbiology 2023Quote: ... filtered HEK293F supernatants containing the expressed proteins were flowed (0.5 -1.0 mL/min) over Econo-Column chromatography columns (Bio-Rad, CA, USA) containing 1-2 mL of agarose bound GNL (Vector Laboratories) ...
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bioRxiv - Cancer Biology 2023Quote: ... The supernatant was collected and quantified using the Bio-Rad Protein Assay Kit and the Quick Start Bovine Serum Albumin Standard Set (Bio-Rad) and stored at −80°C before use.
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bioRxiv - Molecular Biology 2023Quote: ... containing β-mercaptoethanol (1/10 in 4X buffer) and transfer of protein onto PVDF or nitrocellulose using the Trans-Blot Turbo System (Bio-Rad). Secondary HRP-conjugates were visualized after addition of ECL Prime Western Blotting Substrate and chemiluminescent detection in an Amersham Imager 680 or fluorescence detection using a LICOR Odyssey CLx ...
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bioRxiv - Molecular Biology 2023Quote: ... Five micrograms of total protein were resolved by SDS-PAGE on a 10% gel under reducing conditions and transferred to PVDF membranes (Bio-Rad). The blots were blocked in TBS containing 5% non-fat milk for 30 mins and incubated with SARS-CoV-2 nucleocapsid protein antibody (1:1000 ...
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bioRxiv - Physiology 2023Quote: ... The protein extract (40μg) was separated on 8 or 10% polyacrylamide mini gel and transferred to a PVDF membrane (Bio-Rad, USA). Blotted membranes were incubated overnight at 4°C in SuperBlock(PBS ...
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bioRxiv - Microbiology 2023Quote: ... 10% ß-mercaptoethanol) was used as the starting material for separation on 4–20 % Mini-PROTEAN TGX Stain-Free Protein Gels (Bio-Rad). The purified virus was loaded into the sample well and was run on the gel placed in a Mini-PROTEAN Tetra Vertical Electrophoresis Cell for 30 min at 200 V ...
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bioRxiv - Genetics 2023Quote: ... The gels were run until proper band separation was achieved and the protein was transferred onto a 0.2 µm nitrocellulose membrane using the wet-transfer method (Bio-Rad Laboratories). The membranes were blocked with Odyssey Blocking Buffer (LiCOR Biosciences ...
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bioRxiv - Cell Biology 2023Quote: ... A total of 20 μg protein was denatured at 95 °C for 5 min and loaded on a 4-20% precast polyacrylamide gel (BioRad, 4568094) for gel electrophoresis ...
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bioRxiv - Cell Biology 2023Quote: ... cleared supernatant was transferred to the new clean Eppendorf tubes and total protein concentration was measured using Bradford assay by Bio-Rad Protein Assay kit (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... About 15 μg of protein samples from lysates were boiled at 95°C for 5 min in Laemmli sample buffer (Bio-Rad) and resolved in 4-15% gradient SDS-PAGE gels (Mini-Protean TGX ...
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bioRxiv - Immunology 2023Quote: ... 1min activation of the gel and imaging of total protein on the blot was performed by a Gel Doc EZ Imager (Bio-Rad). Blots were blocked in Intercept blocking buffer (PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein lysates were run on 10% sodium dodecyl sulfate-polyacrylamide gels (SDS-PAGE) and transferred to a nitrocellulose membrane (Bio-Rad) using a wet tank blotting system ...
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bioRxiv - Neuroscience 2023Quote: ... Equal amounts of proteins (20–100μg as optimal for each antibody) for each sample was loaded into 4-15% Mini-PROTEAN TGX Stain-Free Protein Gels (BioRad; #4568084) for electrophoresis ...
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bioRxiv - Neuroscience 2023Quote: ... Protein (10 ug) from each sample was treated with ß-mercaptoethanol and subsequently electrophoresed on precast 4%–10% gradient gels (Biorad), as previously described (Alcantara et al. ...
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bioRxiv - Biochemistry 2023Quote: ... Thermal stability of the protein samples was measured in a CFX96 Dx Real-Time qPCR instrument (Bio-Rad, Hercules, CA, USA); program ...
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bioRxiv - Bioengineering 2023Quote: ... Equal amounts of the proteins (50 µg) were electrophoresed (125 volts, 1 h) on a 4-20% Mini protean TGX stain-free protein gel (Bio-Rad) and transferred onto nitrocellulose membranes (125 volts ...
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bioRxiv - Cell Biology 2023Quote: ... 20-40 μg of total protein was resolved on 10% SDS-PAGE gels followed by immunoblotting on PVDF membranes (Bio-rad). Membranes were developed by chemiluminescence (SuperSignal West Pico ...
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bioRxiv - Biochemistry 2022Quote: NKX2-5 homeodomain purity was evaluated through SDS-PAGE using 15 well Mini-PROTEAN TGX Precast Protein Gels® (Bio-Rad). Samples were prepared with 4X loading buffer containing β-mercaptoethanol (BME ...
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bioRxiv - Cell Biology 2023Quote: ... for protein extracted from cultured cells or brain sections and micro bicinchoninic acid (microBCA) for protein extracted from EVs according to the manufacturer’s instructions (Bio-Rad Laboratories).
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bioRxiv - Cancer Biology 2023Quote: ... and the samples were boiled for 5 minutes before being loaded on the gel (4-15% Criterion TGX Precast Midi Protein Gel, Bio-Rad). Proteins were separated by SDS-PAGE and blotted into a polyvinylidene fluoride (PVDF ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein samples were subjected to 10–12% SDS-PAGE and transferred to a polyvinylidene fluoride membrane (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Cell Biology 2023Quote: ... Protein content was confirmed by mixing 2 μL of each elution step with 200 μL Bradford solution (Protein assay dye reagent (Bio-Rad) diluted 1:5 in H2O) ...
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bioRxiv - Molecular Biology 2023Quote: ... and Precision Plus Protein Unstained Standards (#1610363) was resolved by SDS-PAGE using precast 4-20% MiniProtean TGX Stain Free Gels (Bio-Rad). The blot in Fig ...
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bioRxiv - Microbiology 2022Quote: ... and proteins were analysed either by western blot or by silver staining after electrophoretic separation on polyacrylamide SDS-PAGE gel (Bio-Rad 4–20% Criterion™ TGX Stain-Free™ Protein Gel ...
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bioRxiv - Bioengineering 2023Quote: ... Approximately 5 x 108 vesicles and purified HaloTag standard curve were loaded and run on a 12% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad) at 150 V for 90 min or 50V for 10 min followed by 100V for approximately 70 min ...