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Citations for Bio-Rad :
8751 - 8800 of 9412 citations for Recombinant Human BNIP3 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... and 30 µg of total protein extract were loaded on 10% acrylamide-bis-acrylamide gels or 4-20% Mini-PROTEAN TGX Precast Gels (Bio-Rad). Proteins were separated by migration at 135 V in 1X Tris-Glycine-SDS buffer (Euromedex) ...
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bioRxiv - Cancer Biology 2024Quote: ... The insoluble material was separated at 13,000 rpm for 30 minutes at 4 °C and total protein concentration was measured on supernatants using Bradford reagent (Bio-Rad). Protein extracts were denatured with NuPAGE®LDS (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... proteins incubated with Mal-B were electrotransferred to PVDF membranes using a Trans-blot SD semi-dry transfer cell (Bio-Rad). Biotin incorporation was assessed by incubating blots with HRP-streptavidin (1:1000 v/v ...
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bioRxiv - Microbiology 2024Quote: ... Proteins of interest (FtsZ, FtsN and MinD) were transferred to nitrocellulose membranes using a semi-dry Transfer-Blot apparatus (Bio-Rad). The Nitrocellulose membranes were then blocked in 5% (w/v ...
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bioRxiv - Immunology 2024Quote: ... Equal amounts of pre-cleared lysate samples were separated in 8 – 16% Mini protean-TGX stain free protein gel (Bio-Rad) and the gel was transferred to a Trans-Blot Turbo Mini-PVDF membrane (Bio-Rad ...
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bioRxiv - Immunology 2024Quote: ... the proteins were transferred to a polyvinylidene difluoride membrane (110V, 1h, 4°C) using a Mini Trans-Blot cell apparatus (Bio-Rad). Non-specific binding sites were blocked using PBS/Tween 20 (0.05% Tween 20 ...
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bioRxiv - Biochemistry 2023Quote: ... Approximately 20-30 μg of protein was resolved on 8% polyacrylamide gels and transferred onto nitrocellulose membranes using semi-dry Trans-Blot TurboTM (Bio-Rad). Following antibody incubation (anti-GFP ...
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bioRxiv - Biochemistry 2024Quote: ... and 5 to 20 mM imidazole (wash-buffer) until the eluate was protein-free as judged by the lack of reaction with Bradford dye reagent (Bio-Rad). The calpain-3-titin/I81-I83 complex was eluted with 300 mM imidazole in wash-buffer ...
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bioRxiv - Biochemistry 2024Quote: ... the beads were mixed with 1.5 × loading buffer and then loaded on a 4-15% pre-casted protein gels (BioRad; #456-1084) prior to transfer on PVDF membrane for western blotting using anti-Flag ...
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bioRxiv - Biophysics 2024Quote: ... Growth media was exchanged after 24 hours and cells were checked for protein expression using a fluorescent cell imager (Bio-Rad). Cells were harvested after another 24 hours in cold PBS before purification ...
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bioRxiv - Bioengineering 2024Quote: ... The samples were then loaded onto the gel and run on ice for 45 min at 150 V using 10 µL Precision Plus Protein Standards All Blue (Bio-Rad) as ladder ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins transferred from SDS-polyacrylamide gels to nitrocellulose membranes were visualized using a ChemiDoc analysis system (Bio-Rad, Hercules, CA, USA). Several exposures were analyzed to determine the linear range of the chemiluminescence signals ...
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bioRxiv - Cancer Biology 2024Quote: ... 30 µg of protein lysate were then run per lane on a 10% polyacrylamide gel (Bio-Rad Laboratories, Hercules, CA, USA) and transferred to a PVDF membrane (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2024Quote: ... Equal amounts of protein (20-30 μg) were resolved by 8 to 15% SDS PAGE MiniPROTEAN® TGX gels (Bio-Rad), from samples previously prepared with Laemmli Buffer 4X™ (Bio-Rad ...
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bioRxiv - Biophysics 2024Quote: ... The reaction was then centrifuged at 14,000rpm for 5 minutes and the Ire1-LD concentrations in supernatant and pellet (after washing three times in HMK buffer) were measured using the Bradford protein assay (Bio-Rad) with bovine serum albumin as a standard ...
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bioRxiv - Biophysics 2024Quote: ... supernatant was filtered with 0.45 µm filter (Fisher) and loaded to HiTrap Q column (Cytiva) on an NGC Fast Protein Liquid Chromatography (FPLC) station (Bio-Rad). The flow-through fraction was mixed with CM Sepharose FF resin (GE HealthCare Lifesciences ...
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bioRxiv - Neuroscience 2023Quote: Cell cultures and animal tissues: 15 μg of protein extracts were loaded on 10% pre-cast gels (Bio-Rad Cat.# 4568034) and run for 1hr at 100V ...
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bioRxiv - Biochemistry 2024Quote: ... the absorbance of standards and protein samples was measured at 655 nm using a microplate absorbance reader (Bio-Rad Model 680). See Table S4 for raw Lowry assay data.
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bioRxiv - Biochemistry 2024Quote: ... the absorbance of standards and protein samples was measured at 550 nm using a microplate absorbance reader (Bio-Rad Model 680). See Table S5 for raw BCA assay data.
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bioRxiv - Molecular Biology 2023Quote: ... 40 μg of protein extract was loaded into a 10% TGX Stain-Free FastCast Acrylamide Gel (Bio-Rad, Hercules, CA, USA) or SDS polyacrylamide 15% running gel in the case of histone analysis ...
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bioRxiv - Molecular Biology 2023Quote: ... and light units were normalized by the total protein concentrations of the corresponding WCEs determined using the Bradford reagent (BioRad, 5000006).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... before being loaded on a SDS-PAGE gel (4–20% Criterion™ TGX Stain-Free™ Protein Gel, Bio-Rad #5678095). Western blot was then performed as described in the previous section.
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bioRxiv - Plant Biology 2023Quote: ... The proteins were then transferred to polyvinylidene difluoride membranes using NuPAGE Transfer Buffer using a Trans-Blot Turbo Transfer System (Bio-Rad) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were denatured by boiling for 10 minutes in the DTT containing sample buffer and separated by 4-20% Mini-PROTEIN TGX gels (BIO-RAD) and then transferred to the 0.2 μm PVDF membrane (BIO-RAD) ...
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bioRxiv - Biochemistry 2023Quote: ... Cyt-b5 and other protein band intensities were determined using the volume contour tool Image Lab (Bio-Rad; Hercules, CA, USA) or ImageJ software ...
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bioRxiv - Cell Biology 2023Quote: ... Equal amounts of total protein samples (40-50 µg) were resolved by polyacrylamide gels using self-made or pre-cast gels (Bio-Rad). Proteins were electroblotted on nitrocellulose membranes (Cytiva ...
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bioRxiv - Synthetic Biology 2023Quote: ... The purity and size of the proteins were validated on an SDS-PAGE gel (Mini-PROTEAN TGX and Mini-TETRA cell, Bio-Rad). Purified proteins were stored at 4° C.
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bioRxiv - Molecular Biology 2023Quote: ... proteins were transferred from the unstained gel to polyvinylidene fluoride membranes using the Trans-Blot turbo transfer system (Bio-Rad Laboratories). An iBind™ Flex system (Invitrogen/ThermoFischer ...
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bioRxiv - Synthetic Biology 2023Quote: ... 10 μL of each sample was separated on an Any kD™ Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad). Proteins were electrophoretically transferred to a PVDF membrane using a Trans-Blot® Turbo™ Transfer System (BioRad ...
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bioRxiv - Cell Biology 2023Quote: ... Approximately equal loads of extracted protein were subjected to SDS-PAGE and transferred to a 0.2 µm nitrocellulose membrane (Bio-Rad 1620112). Transferred proteins were then stained with Ponceau S Solution (Sigma-Aldrich P3504) ...
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bioRxiv - Microbiology 2023Quote: ... 12 % SDS-PA gels were fixed with fixation solution (40 % ethanol, 10 % acetic acid) o/n and stained in Flamingo fluorescent protein dye (Bio-Rad) for up to 6 h and imaged with the ChemiDoc (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: ... The blots were washed and the labelled protein bands were visualized by using Pierce ECL reagent and a ChemiDoc XRS+ system (Bio-Rad).
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bioRxiv - Cancer Biology 2022Quote: 40 µg of proteins were separated using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) on a 10% acrylamide gel (BIO-RAD) and transferred to nitrocellulose membrane by semi-dry blotting using the Trans-Blot Semi-Dry Transfer Cell (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... containing β-mercaptoethanol (1/10 in 4X buffer) and transfer of protein onto PVDF or nitrocellulose using the Trans-Blot Turbo System (Bio-Rad). Secondary HRP-conjugates were visualized after addition of ECL Prime Western Blotting Substrate and chemiluminescent detection in an Amersham Imager 680 or fluorescence detection using a LICOR Odyssey CLx ...
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bioRxiv - Molecular Biology 2023Quote: ... Five micrograms of total protein were resolved by SDS-PAGE on a 10% gel under reducing conditions and transferred to PVDF membranes (Bio-Rad). The blots were blocked in TBS containing 5% non-fat milk for 30 mins and incubated with SARS-CoV-2 nucleocapsid protein antibody (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... 20 µg of the protein lysate were mixed with 7.5 µl of 4X Laemmli sample buffer (Bio-Rad Laboratories, Cat #: 1610747) containing 10% β-mercaptoethanol (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... cleared supernatant was transferred to the new clean Eppendorf tubes and total protein concentration was measured using Bradford assay by Bio-Rad Protein Assay kit (Bio-Rad) ...
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bioRxiv - Cancer Biology 2023Quote: ... The supernatant was collected and quantified using the Bio-Rad Protein Assay Kit and the Quick Start Bovine Serum Albumin Standard Set (Bio-Rad) and stored at −80°C before use.
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bioRxiv - Cell Biology 2023Quote: ... The lysates were centrifuged at 18,000 x g for 30 minutes at 4 °C and the supernatant was incubated with SurebeadsTM Protein A Magnetic Bead (Bio-rad) conjugated with rabbit monoclonal DYKDDDDK antibody at RT for two hours ...
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bioRxiv - Neuroscience 2023Quote: ... 10-30 μg of protein extracts were run at 100V for 1 hr on 10% pre-cast SDS-PAGE gels (Bio-Rad), then electro-transferred to 0.2 μm nitrocellulose membranes using the Trans-Blot Turbo Transfer system (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... A total of 20 μg protein was denatured at 95 °C for 5 min and loaded on a 4-20% precast polyacrylamide gel (BioRad, 4568094) for gel electrophoresis ...
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bioRxiv - Immunology 2023Quote: ... 1min activation of the gel and imaging of total protein on the blot was performed by a Gel Doc EZ Imager (Bio-Rad). Blots were blocked in Intercept blocking buffer (PBS ...
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bioRxiv - Genetics 2023Quote: ... The gels were run until proper band separation was achieved and the protein was transferred onto a 0.2 µm nitrocellulose membrane using the wet-transfer method (Bio-Rad Laboratories). The membranes were blocked with Odyssey Blocking Buffer (LiCOR Biosciences ...
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bioRxiv - Microbiology 2023Quote: ... 10% ß-mercaptoethanol) was used as the starting material for separation on 4–20 % Mini-PROTEAN TGX Stain-Free Protein Gels (Bio-Rad). The purified virus was loaded into the sample well and was run on the gel placed in a Mini-PROTEAN Tetra Vertical Electrophoresis Cell for 30 min at 200 V ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated by isoelectric focusing on immobilized linear pH gradient strips (pH 5 to 8, Bio-Rad, Hercules, CA, USA) before separation by SDS-PAGE ...
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bioRxiv - Microbiology 2023Quote: ... filtered HEK293F supernatants containing the expressed proteins were flowed (0.5 -1.0 mL/min) over Econo-Column chromatography columns (Bio-Rad, CA, USA) containing 1-2 mL of agarose bound GNL (Vector Laboratories) ...
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bioRxiv - Pathology 2023Quote: ... total proteins were extracted and examined as previously described.59 The blotting was performed on the PVDF membrane (Bio-Rad, USA) and imaged with the Western Blotting Detection Kit (Advansta ...
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bioRxiv - Cell Biology 2023Quote: ... Band intensities were normalized to total protein in the sample quantified within each lane using Stain-Free Dye Imaging (Bio-Rad).
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bioRxiv - Cancer Biology 2023Quote: ... Sodium-dodecyl-sulfate polyacrylamide gel electrophoresis and transfers of proteins to .45uM nitrocellulose membranes were performed using the Protean 2 or 3 systems (Bio-Rad) and protocols provided by the manufacturer ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated by SDS-PAGE and transferred onto PVDF membranes applying the Trans-Blot Turbo Transfer system (Bio-Rad Laboratories). Membranes were blocked with 5% BSA or 5% NFDM in 1X TBS-T buffer and incubated with specific primary and secondary antibodies ...