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Citations for Bio-Rad :
8751 - 8800 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... cells were lysed in 300ul 1X sample buffer (BioRad) and boiled for 5 min at 95 C and loaded onto an or 4-20% TGX stain-free polyacrylamide gels (Biorad) ...
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bioRxiv - Cell Biology 2023Quote: ... Individual oxidized proteins were resolved by SDS-PAGE on 4-20% polyacrylamide gel gradient gel (BioRad, 5671093). Resolved proteins were transferred to PVDF membrane at 75 V for 2h ...
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bioRxiv - Cell Biology 2023Quote: ... JFX650 was measured on ChemiDoc imaging system (BioRad) using the Cy5 filter set following by the stainfree loading control ...
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bioRxiv - Cell Biology 2023Quote: ... Gels were transferred using the Trans-Blot Turbo system (with Turbo transfer buffer, BioRad) and blocked for 30 minutes in 5% milk ...
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bioRxiv - Cell Biology 2023Quote: ... Protein amount was determined by Bradford (Bio-Rad) assay at 595 nm absorbance ...
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bioRxiv - Cell Biology 2023Quote: ... and transferred to a nitrocellulose membrane (Maine Manufacturing, LLC) using a Trans-Blot SD Semi-Dry Transfer Cell apparatus (Bio-Rad). Primary antibodies were a rabbit monoclonal anti-HA (Cell Signaling ...
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bioRxiv - Cell Biology 2023Quote: ... with two individual parent sets and two technical replicates on the CFX96TM Real Time PCR Detection System (Bio-Rad). RpL32 was used as a reference gene ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative PCRs were performed with specific oligonucleotide pairs as indicated in Supplemental Table S1 using the iTaq Universal SYBR Green Supermix (BioRad) and a Rotor-Gene 6000 instrument (Corbett) ...
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bioRxiv - Cell Biology 2023Quote: ... Bands were observed using a ChemiDoc XRS+ (Bio-Rad).
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bioRxiv - Cell Biology 2023Quote: ... Membranes were then exposed to the ECL substrate kit (Pierce 34850 and 34095) for 3 minutes before imaging with the ChemiDoc Touch Imaging System (Bio-Rad).
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bioRxiv - Cell Biology 2023Quote: ... Quantitative analysis was carried out on a CFX384 system (Bio-Rad) using the iQ SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Signal detection was performed by an enhanced chemiluminescence detection reagent (Bio-Rad). Approximate molecular masses were determined by comparison with the migration of prestained protein standards (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... using the iQ SYBR Green Supermix (Bio-Rad) as directed by the manufacturer.
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bioRxiv - Cell Biology 2023Quote: Quantitative PCR (qPCR) reactions were carried out in triplicate on specific genomic regions using SYBR Green Supermix (Bio-Rad). The resulting signals were normalized for primer efficiency by carrying out qPCR for each primer pair using Input DNA.
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bioRxiv - Cell Biology 2023Quote: ... after which they were transferred onto PVDF membranes (Bio-Rad 1704156).The membranes were blocked using 5% BSA (Rockland BSA-50 ...
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bioRxiv - Cell Biology 2023Quote: ... and goat anti-mouse IgG (H+L)-HRP conjugate (170-6516, Biorad, 1:10000). Antibody binding was detected by the enhanced chemiluminescence system (Thermo Fischer Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... The quantification of mRNA expression was performed using the SYBR Green dye (Bio-Rad SsoAdvanced - Universal SYBR Green Supermix ...
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bioRxiv - Cell Biology 2023Quote: ... and then transferred to the 0.2 µm polyvinylidene difluoride (PVDF) membrane (Bio-Rad). PVDF membrane was further blocked by TBST (TBS buffer with 0.1% Tween-20 ...
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bioRxiv - Cell Biology 2023Quote: ... Triton X-100 is from Bio-Rad. Rabbit anti-phospho PLB (Ser16/Thr17 ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were run through a 28 gauge needle and were electrophoresed on Criterion gels (Bio-Rad) and transferred to nitrocellulose using a Trans-Blot Turbo (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: cDNA was synthesized using an iScript cDNA synthesis kit (BioRad). Real-time PCR was carried out on a ViiA 7 Real-Time PCR System (Thermofisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... 1:5000 anti-Mouse HRP antibody (Bio-rad,5178-2504) and 1:5000 anti-Rabbit HRP antibody (Bio-rad,5196-2504) ...
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bioRxiv - Cell Biology 2023Quote: ... and extracted in 500 µL 1X Laemmli Sample Buffer (Bio-Rad, 1610737). The samples were then boiled at 90◦C for 5 min ...
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bioRxiv - Cell Biology 2023Quote: ... Blots were imaged using a ChemiDoc Touch (Bio-Rad). Densitometry was performed using ImageJ.
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bioRxiv - Cell Biology 2023Quote: ... Quantitative mRNA expression was determined by real-time RT-PCR using iTaq Universal SYBR Green Supermix (Bio-Rad, 172-5124). Primers used were ...
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bioRxiv - Cell Biology 2023Quote: ... Chemiluminescent (Amersham ECL Prime, #RPN2232) detection was performed according to the manufacturers guidelines (Bio-Rad, Chemidoc Imaging System). Blots were images using the Bio-Rad ChemiDoc imaging System.
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bioRxiv - Cell Biology 2023Quote: ... Proteins were then transferred using a Trans-Blot Turbo Transfer System and the Trans-Blot Turbo Mini Nitrocellulose Transfer Packs (1704158, Bio-Rad), which were blocked with 5% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA was generated using the iScript cDNA synthesis kit (Bio-Rad) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... primer and cDNA reactions were executed using iTaq Mastermix (Bio-Rad, #172-5171). Ct values (mean cycle threshold ...
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bioRxiv - Cell Biology 2023Quote: ... Protein concentration was measured using Bradford reagent (BioRad 5000006).
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bioRxiv - Cell Biology 2023Quote: ... DC assay (#5000116) was purchased from BioRad. Cells were routinely tested for mycoplasma and were never positive ...
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bioRxiv - Cell Biology 2023Quote: ... Immune complexes were detected with appropriate HRP-conjugated secondary antibodies from Cell Signaling Technologies and enhanced chemiluminescence reagent (Clarity ECL, BioRad). Protein band intensities were quantified using ImageJ Software.
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies used for Western blotting were goat anti-mouse- and anti-rabbit-HRP (#172-1011 and #170-6515, Bio-Rad, USA).
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bioRxiv - Cell Biology 2023Quote: ... RNA was reverse transcribed using the iScript Reverse Transcription Supermix (Bio-Rad), and cDNA was stored at −20 until use ...
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bioRxiv - Cell Biology 2023Quote: ... Analysis of Western blotting was conducted using ImageLab (Bio-Rad). The area of segmented focal adhesions was measured using Analyze Particles in ImageJ.
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bioRxiv - Cell Biology 2023Quote: ... and the specific proteins were detected and visualized by ChemiDoc Touch (Bio-Rad).
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bioRxiv - Cell Biology 2023Quote: ... Denatured samples were then loaded and run on Criterion TGX (Tris-Glycine eXtended) precast gels (Bio-Rad) for ∼15 minutes at 80V ...
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bioRxiv - Cell Biology 2023Quote: ... PCR products were run at 135V on a 1% agarose gel containing ethidium bromide and bands were observed using a ChemiDoc XRS+ (Bio-Rad). Sequencing of PCR products was performed by Psomagen and resulting sequencing files were analyzed with Geneious software using NCBI gene data.
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bioRxiv - Cell Biology 2023Quote: ... Gels were then separated from walls and bottom of the well and imaged every 30 mins for 1.5 hrs using a chemi-doc imaging platform (Bio-Rad). This first 1.5 hrs was considered the spontaneous contraction phase (SCP) ...
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bioRxiv - Cell Biology 2023Quote: ... The samples were run on Any kD™ Mini-PROTEAN® TGX™ gel (Bio-Rad Laboratories) and then dry transferred onto a nitrocellulose membrane using the iBlot system (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... The percent knockdown for each gene in question was derived from two individual parent sets and each of these biological replicates was run with two technical replicates on the CFX96 Real Time PCR Detection System (Bio-Rad) for a total of four samples per gene ...
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bioRxiv - Cell Biology 2023Quote: ... with two individual parent sets and two technical replicates on the CFX96TM Real Time PCR Detection System (Bio-Rad). RpL32 was used as a reference gene ...
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bioRxiv - Cell Biology 2023Quote: ... 93.75 µL of 40% acrylamide (Bio-Rad), 25 µL of 2% bis-acrylamide (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... RT-PCR analysis was performed using the iTaq™ Universal SYBR® Green Supermix (Bio-Rad) with two individual parent sets and two technical replicates on the CFX96TM Real Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... P4 and final supernatant were separated on a 15% Criterion Tris-glycine gel (BioRad) followed by transfer to PVDF membrane as previously described (4) ...
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bioRxiv - Cell Biology 2023Quote: ... RT-PCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad) and primers obtained from the Fly Primer Bank listed in Table S1 ...
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bioRxiv - Cell Biology 2023Quote: ... 1 µg of total RNA was used for reverse transcription with the iScript™ gDNA Clear cDNA Synthesis Kit (Bio-Rad). RT-PCR analysis was performed using the iTaq™ Universal SYBR® Green Supermix (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Equal amounts of protein lysates (20 µg) were separated in 10% SDS-PAGE gels and transferred to PVDF membranes (Bio-Rad). The membranes were incubated with specific primary antibodies (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... Bradford assay (Biorad) was performed on the clarified lysates to determine the protein concentrations and all samples were diluted in lysis buffer and SDS loading buffer (final concentrations ...
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bioRxiv - Cell Biology 2023Quote: ... Concentrated conditioned medium was fractionated by size exclusion chromatography (SEC) by application to Sepharose CL-2B resin (17014001, Cytiva) stacked in a disposable Econo-Pac column (7321010, Bio-Rad). The column was eluted with PBS and 0.5 ml fractions were collected ...