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Citations for Bio-Rad :
8601 - 8650 of 9412 citations for Recombinant Human PDCD1LG2 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... with a molecular weight cut off of 10 kDa and the final concentration was estimated using the Bradford protein assay (5000006, Bio-Rad). The protein was also subjected to mass spectrometry to assess its purity and molecular weight ...
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bioRxiv - Biochemistry 2019Quote: ... Production and purification of the isopeptide linked Ubc13~Ub conjugate was analysed by SDS-PAGE and the FRET labelled protein was imaged using ChemiDoc MP (Bio-rad) with Cy3 and AlexaFluor 647 filter settings ...
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bioRxiv - Physiology 2020Quote: ... Transfer of the proteins to the PVDF membranes was performed using the Trans-Blot TurboTM Transfer System (BioRad, Hercules, CA, USA). Membrane was blocked for 1h at RT (5% milk in TBS-T ...
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bioRxiv - Plant Biology 2019Quote: Equal amounts of the solubilized protein fractions were separated on 10% SDS-PAGE gels and blotted onto PVDF membranes (Bio-Rad). RFP-fused proteins were detected using a rat anti-RFP antibody (1:5,000 ...
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bioRxiv - Plant Biology 2019Quote: ... Equal amounts of the solubilized protein fractions were separated on 10% SDS-PAGE gels and blotted onto PVDF membranes (Bio-Rad). Protochlorophyllide oxidoreductase (POR ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 7 μg of nuclear protein extract was separated by SDS-PAGE and transferred to PVDF membrane (Trans-Blot Turbo System, Bio-Rad). We followed standard immunoblot procedures using primary antibodies raised against the Xpress tag (ThermoFisher R910-25 ...
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bioRxiv - Bioengineering 2019Quote: ... The fractions were analyzed by SDS-Page and fractions containing the protein of interest were pooled and loaded onto a 5 mL Mini-profinity IMAC column (Bio-Rad). Metal affinity chromatography was performed as previously described for the TEV protease ...
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bioRxiv - Molecular Biology 2019Quote: ... Comparative ΔCt normalized to gamma actin and 60S ribosomal protein L35 was used to calculate transcription levels by using the geNorm algorithm automated in CFX manager software (Bio-Rad).
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bioRxiv - Pathology 2020Quote: ... The correct size of the purified RBD-scFv protein (52.4 kDa → ∼57 kDa with glycosylation) was confirmed by protein gel electrophoresis using Mini-PROTEAN TGX Precast Gels 4-20% (Bio-RAD).
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bioRxiv - Cancer Biology 2019Quote: ... on an orbital shaker after blocking with milk and after each probing step. Finally, the membrane was treated with chemiluminescent substrate (ThermoScientific, cat. no. 34080) and protein bands were detected by Bio-Rad Chemidoc imaging system.
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bioRxiv - Plant Biology 2020Quote: ... heated for 30 min at 37°C and loaded into a polyacrylamide gel (any kD™ precast protein gel, Biorad #4569034). To avoid cross-contamination ...
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bioRxiv - Cell Biology 2019Quote: Tissue or cell extracts equivalent to 20 μg total protein were resolved in 10% SDS-PAGE gels followed by electrophoretic transfer onto a nitrocellulose membrane (Bio-Rad) in Tris-glycine buffer ...
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bioRxiv - Synthetic Biology 2020Quote: ... 10 to 25 μg for experiments that were imaged digitally) was loaded onto a 4–15% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad) and run either at 50 V for 10 min followed by 100 V for at least 1 h ...
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bioRxiv - Microbiology 2020Quote: ... The proteins were transferred to a Polyvinylidene difluoride (PVDF) membrane using the Trans-Blot® Turbo™ Transfer System (Bio-Rad). Detection of GFP was carried out with a rabbit polyclonal anti-GFP antiserum (Bio-Rad ...
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bioRxiv - Developmental Biology 2021Quote: ... Blots were then washed 3x 10 min in TBS followed by imaging of Stain Free total protein signal with a ChemiDoc MP System (Bio-Rad). Following stain-free imaging ...
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bioRxiv - Molecular Biology 2021Quote: ... samples were subjected to SDS-polyacrylamide gel electrophoresis and proteins were blotted onto nitrocellulose membranes using the Mini Trans-Blot Cell system (Bio-Rad).
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bioRxiv - Neuroscience 2020Quote: ... Bovine Serum Albumin (BSA) with concentrations 0.125 to 2.0mg/mL from a standard set served as protein standards (BioRad, Cat. Number 5000207). Then ...
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bioRxiv - Synthetic Biology 2019Quote: ... The purity and size of the proteins were validated on SDS-PAGE gel (Mini-PROTEAN TGX and Mini-TETRA cell, Bio-Rad). Purified proteins were stored at –20° C ...
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bioRxiv - Genomics 2019Quote: All protein isolation steps were performed at 4 °C or on ice and protein concentrations were assessed with the Bradford assay (Bio-Rad). Protein from total pancreas lysates was isolated from a small fragment of mouse pancreas and minced in lysis buffer (20 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2020Quote: ... The lysates were loaded onto 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (Cat# 4561086, Bio-Rad, Hercules, CA), and proteins were subsequently transferred onto a polyvinylidene fluoride (PVDF ...
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bioRxiv - Synthetic Biology 2021Quote: ... Total proteins were separated in SDS-PAGE and transferred to a PVDF membrane using Transblot turbo (Bio-Rad Laboratories, CA, USA). After blocking the membrane with 0.3% skim milk in TBS-T ...
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bioRxiv - Synthetic Biology 2021Quote: ... The purity and size of the proteins were validated on a SDS-PAGE gel (Mini-PROTEAN TGX and Mini-TETRA cell, Bio-Rad). Purified proteins were stored at –20° C.
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bioRxiv - Microbiology 2020Quote: ... Samples were checked for purity and presence of protein using 15% SDS-PAGE (Polyacrylamide gel electrophoresis, Mini Protean 3 System, Bio-Rad) and Coomassie Brilliant Blue (Merck ...
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bioRxiv - Microbiology 2020Quote: ... proteins were blotted onto nitrocellulose membranes (Whatman, Maidstone, United Kingdom) using a Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad) under conditions suggested by the manufacturer ...
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bioRxiv - Microbiology 2020Quote: ... Protein bands for both of the gels were transferred to polyvinylidene fluoride (PVDF) membranes (Immun-Blot® 0.2 μm, Bio-Rad) using semi-dry electroblotting system (Trans-Blot® TurboTM Transfer System ...
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bioRxiv - Microbiology 2020Quote: ... The protein concentration of the supernatant was determined by Bradford measurement according to the manufacturer’s instructions (Bio-Rad, cat n° 5000006).
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bioRxiv - Microbiology 2020Quote: ... The protein samples were separated by either 15% SDS-glycine-PAGE or SDS-tricine-PAGE including the Precision Plus Protein Dual Xtra molecular weight marker (Bio-Rad). To run samples under nonreducing conditions ...
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bioRxiv - Biophysics 2020Quote: ... 15 μg of proteins were loaded onto gels prior to conducting SDS-PAGE and transferring onto polyvinylidene difluoride membranes (Bio-Rad). Membranes were then incubated with monoclonal antibodies against Cyclin B1 (PC133 ...
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bioRxiv - Neuroscience 2019Quote: ... Dynabead-antibody complexes were washed 3x with 1 ml IP buffer and 3x with 1X PBS and proteins were eluted at 70°C for 10 min in 2X Laemmli sample buffer (50 μl; Bio-Rad) containing 5% 2-mercaptoethanol ...
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bioRxiv - Neuroscience 2020Quote: ... following the manufacturer’s instructions and 12 µg of protein were loaded into 4-15% gradient gels (Biorad Miniprotean TGX, Biorad, Munich, Germany) and transferred to nitrocellulose membranes ...
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bioRxiv - Microbiology 2021Quote: ... 5 µL of samples were loaded into a precast 4-20% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) gels (Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad) alongside size standards (Precision Protein Plus ...
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bioRxiv - Immunology 2021Quote: 50 ug of protein was prepared in Protein Sample Loading Buffer (Li-Cor Biosciences) and loaded on Mini-PROTEAN 4-20% TGX Stain-Free Precast Gels (Bio-Rad). Immunoblotting was performed as detailed in the Supplementary Materials and Methods ...
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bioRxiv - Immunology 2020Quote: ... Samples were boiled for 3 min and sonicated for 30 s prior to loading on 4-20% Mini-PROTEAN TGX precast protein gels (Bio-Rad) and electrophoresis in Tris-glycine buffer ...
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bioRxiv - Physiology 2019Quote: ... The total protein (about 50 μg) was separated in an SDS-PAGE (10 %) and transferred onto nitrocellulose membrane (Bio-Rad, America). The membranes were blocked with TBST (137 mM NaCl ...
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bioRxiv - Developmental Biology 2019Quote: ... were used to standardize amount of total protein loaded on a gel 12% polyacrylamide gel was then transferred to polyvenyidene fluoride (PVDF membrane, Bio-Rad). 5% nonfat dry milk in PBST (Phosphate buffered saline with Tween 20 ...
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bioRxiv - Genetics 2019Quote: ... Separated proteins were transferred to PVDF membrane at 350 mA constant for 90 minutes using the Mini-TransBlot system (Bio-Rad). Transfer was validated by ponceau stain (Sigma) ...
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bioRxiv - Genomics 2019Quote: The volume for approximately the same number of cells for each sample across the mitotic release time course was loaded in each lane of a 4-12% bis-tris protein gel (Biorad 3450125) and separated in 1X MES running buffer (Biorad 1610789) ...
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bioRxiv - Cell Biology 2021Quote: ... 5 μg of plasmid DNA corresponding to each protein of interest was thoroughly mixed with 500 μL of the resuspended cells in an electroporation cuvette (Bio-Rad). This cuvette was subject to an electroporation pulse (280 V ...
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bioRxiv - Cell Biology 2020Quote: Transfected cells were lysed in RIPA buffer on day 4 and proteins extracted were separated by 10% - 15% SDS polyacrylamide gel and transferred to Nitrocellulose membranes (Bio-Rad) at 100V for 1 hour and blocked for 1 hour with 5% non-fat milk (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... Fractions containing IADs and OAD were determined by SDS-PAGE (4-20% Mini-PROTEAN TGX precast protein gels run in SDS buffer (Bio-Rad)) ...
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bioRxiv - Physiology 2021Quote: ... and 30 μg of protein per sample was electrophoresed by SDS PAGE on 4–20% Mini-PROTEAN TGX Stain-Free Precast Gels (Bio-Rad) and transferred to polyvinylidene fluoride membranes (Millipore) ...
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bioRxiv - Biochemistry 2020Quote: ... Protein content was determined by BCA assay (Pierce) and proteins were separated by SDS-PAGE and transferred onto nitrocellulose membrane (Bio-Rad). Membranes were blocked ...
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bioRxiv - Cell Biology 2021Quote: ... Protein content was measured by Bradford assay and 30 mg of protein per sample were loaded into 4-20 % precast SDS-PAGE gels (Bio-Rad) under denaturing conditions ...
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bioRxiv - Physiology 2020Quote: ... Twelve micrograms of protein were subjected to SDS-PAGE on 4-20% Criterion TGX stain-free gels (Bio-Rad, Hercules, CA) and transferred to polyvinylidene diflouride membranes (PVDF ...
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bioRxiv - Developmental Biology 2021Quote: Proteins extracted from Padi4CTL and Padi4CKO were subjected to SDS–PAGE (4–20% Mini-PROTEAN® TGX™ Precast gel, Biorad) and then transferred onto a nitrocellulose membrane ...
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bioRxiv - Microbiology 2021Quote: ... the relative amount of each protein was calculated using Quantity One software (densitometer GS-800; Bio-Rad Laboratories, Hercules, CA, USA), and was presented as a percentage of total protein [25].
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bioRxiv - Bioengineering 2021Quote: ... proteins were transferred to a polyvinylidene fluoride (PVDF) membrane (Cytiva) using a Trans-Blot SD Semi-Dry Electrophoretic Cell (Bio-Rad) with 1.5 mA / cm2 membrane for 1 h ...
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bioRxiv - Biochemistry 2021Quote: Equal amount of protein was loaded and resolved on 10% polyacrylamide gel and transferred onto the PVDF membrane supplied by Bio-Rad, USA ...
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bioRxiv - Biochemistry 2021Quote: ... 80 μg of total protein for each sample was resolved by SDS-PAGE prior to transfer to nitrocellulose membranes (Bio-Rad). Membranes were blocked in Tris-buffered saline + 0.1% (v/v ...
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bioRxiv - Biochemistry 2021Quote: ... The recovery of tag-less MlaC was quantified using the ratio standard curve method (41) while that of His-tagged nanodisc-embedded complexes were quantified using the Lowry assay (DC Protein Assay, Bio-Rad). For retrograde assay ...