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Citations for Bio-Rad :
8451 - 8500 of 9925 citations for Recombinant Rabbit IL17F Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Gels were either imaged directly (for fluorescent proteins) or stained with Coomassie blue and then imaged using a ChemiDoc gel imager (BioRad). Gel images were analyzed using Fiji ...
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bioRxiv - Biochemistry 2020Quote: ... The concentration was then measured by DC assay and 20 μg of protein was resolved on an SDS page gel (gradient 4%-20%, BioRad) and transferred to a nitrocellulose membrane ...
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bioRxiv - Neuroscience 2020Quote: ... Protein was transferred to a nitrocellulose membrane (Hybond ECL; Amersham Biosciences) using a semi-dry transfer system (Bio-Rad Laboratories) at 25mV for 45 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... the supernatant of the bacterial lysate was purified by affinity chromatography using an automated Profinia Protein Purification system (Bio-Rad), followed by size-exclusion chromatography using a Superdex 200 Increase 10/300 GL column (GE Healthcare ...
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bioRxiv - Cancer Biology 2021Quote: ... Scientific until approximately 3 cm of separation was obtained between the 25 and 37 kDa protein standards (Bio-Rad; 1610375). Using electrophoresis ...
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bioRxiv - Biophysics 2020Quote: ... Fractions containing protein were pooled and desalted to remove imidazole by gel filtration on a 50 mL BioScale P-6 column (BioRad) using resuspension buffer as the eluant.
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bioRxiv - Biophysics 2021Quote: ... 5 µL of purified (mutant) protein sample was mixed with 1.67 µL of 4X Laemmli loading buffer (Bio-Rad 1610747), then 5 µL was loaded onto an SDS-PAGE gel (Either 12% isocratic (Bio-Rad 4561046 ...
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bioRxiv - Cell Biology 2021Quote: ... 25 µg of protein lysate was heat-denatured at 95°C for 5 minutes in Laemmli sample buffer (Bio-Rad), and then separated using a 4 to 12% Bis-Tris polyacrylamide gel (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: Protein lysates (see “RAS activity assays”) were mixed with 4×Laemmli buffer (Cat.#161-0747; Bio-Rad Laboratories, Hercules, US) containing fresh 200 mM DL-Dithiothreitol (Cat.#3483-12-3 ...
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bioRxiv - Cell Biology 2021Quote: ... The input (15 µg) and eluted proteins (∼2% of IPT) were fractionated in 4−15 % SDS-PAGE gels (Bio-rad) and analyzed by immunoblotting ...
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bioRxiv - Neuroscience 2020Quote: ... PAGE separated proteins were blotted onto a PVDF membrane in a semi-dry Trans-Blot Turbo transfer system (Bio-Rad). The PVDF membrane was blocked with 5 % bovine serum albumin (BSA ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Mini-PROTEAN TDX Precast Protein Gels and Trans-Blot Turbo RTA Mini PVDF Transfer Kit were purchased from Bio-Rad Laboratories (Hercules ...
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bioRxiv - Neuroscience 2020Quote: ... Approximately 12 μg of total protein were loaded onto a 4-20% mini Protean TGX gel (Bio-Rad, Hercules, CA) and blots were evaluated as described previously
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was loaded in a 10-well 4-20% Mini-PROTEAN TGX Precast protein gel (BIO-RAD, Catalog #4561094). The gel was run at 150V for 5 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... The concentrations of the extracts were determined using the Bio-Rad Protein assay kit (Bio-Rad Laboratories Inc. Hercules, CA). The extracts were then aliquoted into individual tubes ...
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bioRxiv - Plant Biology 2020Quote: ... Relative protein levels of three to four biological replicates were quantified using Image Lab™ Software (Bio-Rad, www.bio-rad.com).
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bioRxiv - Microbiology 2020Quote: ... 5 μl of the supernatant was used to normalize the level of total protein in each sample via BCA assay (BioRad) and the supernatant was then flash frozen on dry ice ...
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bioRxiv - Plant Biology 2020Quote: ... separated proteins were transferred onto a membrane by using Trans-Blot® Turbo™ Midi Nitrocellulose Transfer Packs (Bio-Rad) and the TransBlot Turbo device (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... SDS-PAGE gels were run on a Protein III system and transfers were performed with the Trans-Blot Turbo Transfer System (BioRad). Protein bands were detected using a primary antibody followed by an HRP-conjugated secondary antibody (rabbit ...
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bioRxiv - Neuroscience 2021Quote: ... self-cast 10% or 12% SDS-gels or Any kD™ Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad). After electrophoretic separation ...
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bioRxiv - Microbiology 2021Quote: ... Gels were stained with Coomassie Brilliant Blue or proteins were transferred into nitrocellulose membranes in Trans-Blot-Turbo Transfer Buffer (BioRad) for 7 min in a Trans-Blot Turbo Transfer System (BioRad) ...
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bioRxiv - Microbiology 2021Quote: ... The equivalent volume to 10 μg of protein was run in a Stain-Free FastCast 12.5% polyacrylamide gel (Bio-Rad) and transferred to a nitrocellulose membrane using the Trans-Blot Turbo semi-dry system (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: ... Cell lysates were separated by protein electrophoresis at 150 V for 1h using 4-20% Mini-Protean TGX pre-cast gels (BioRad) and transferred by semi-dry technic onto Amersham Hybond-P PVDF membranes (GE Healthcare) ...
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bioRxiv - Neuroscience 2020Quote: ... Separated proteins were then electroblotted using a TransBlot Turbo system to TransBlot Turbo Mini-size PVDF membranes (Bio-Rad, 1704272). Membranes were subsequently blocked with 1x TBS containing 0.1% Tween 20 (1x TBST ...
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bioRxiv - Neuroscience 2019Quote: The amount of protein was prepared from homogenized tissues or tells and determined using the Bradford quantification assay (Bio-Rad). Protein extracts were separated by SDS-PAGE and electroblotted to PVDF membrane (Millipore ...
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bioRxiv - Physiology 2021Quote: ... About 30 μg of protein from the sample extracts were electrophoresed in 12% Mini-PROTEAN TGXTM precast gels (Bio-Rad) using the electrophoresis system mini-PROTEAN Tetra Cell (Bio-Rad) ...
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bioRxiv - Plant Biology 2021Quote: ... 20 µL of each sample was loaded and the proteins were resolved using 10% SDS-PAGE resolving gel followed by transfer to a polyvinylidene difluoride membrane (BioRad) at 100 mA for 12 h ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were transferred onto nitrocellulose membranes using either a Trans-Blot® Turbo™ Transfer System (7 minutes, 25V, BioRad) or an iBlot™ 2 Dry Blotting System (P0 method ...
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bioRxiv - Microbiology 2019Quote: ... proteins were transferred to a polyvinylidene fluoride (PVDF) membrane using the Mini Trans-Blot Electrophoretic Transfer Cell system (Bio-Rad) per the manufacturer’s instructions and blocked with 100 mg/mL skim milk in 1x TBS/Tween 20 ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis using the Mini-PROTEAN® electrophoresis unit (BioRad, Hercules, CA) and transferred to a PVDF membrane (BioRad) ...
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bioRxiv - Neuroscience 2020Quote: ... 15 min at +4°C) and the supernatant was used to measure the protein concentrations using the Lowry method (Biorad DC protein assay ...
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bioRxiv - Microbiology 2021Quote: ... NC-membrane (pore size 0.45 mm) was used for protein transfer using Bio-Rad Mini PROTEAN blotting system (Bio-Rad). Proteins were transferred for 1 h at 300 mA in transfer buffer (25 mM Tris ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein was quantified and the same amount of protein was loaded to the wells in a 4-15% SDS-PAGE gel (BioRad). Protein was transferred to a PVDF membrane (BioRad) ...
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bioRxiv - Cell Biology 2021Quote: ... depending on the proteins analysed) and transferred on 0.2 μm nitrocellulose membranes (Trans-Blot® TurboTM Transfert Pack, Bio-Rad). Equal total proteins loads were confirmed by Ponceau red (0.1% Ponceau ...
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bioRxiv - Cell Biology 2020Quote: Purified ORB proteins were concentrated in a stacking gel using SDS-PAGE stained with Coomassie Brilliant Blue R-250 (Biorad). Proteins in the excised gel band were subjected to in-gel digestion as follows ...
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Pseudohypoxic HIF pathway activation dysregulates collagen structure-function in human lung fibrosisbioRxiv - Cell Biology 2021Quote: ... Immunodetected proteins were identified using the enhanced chemiluminescence system (Clarity Western Blotting ECL Substrate, Bio-Rad Laboratories Ltd, Watford, UK) or Odyssey imaging system (LI-COR) ...
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bioRxiv - Cell Biology 2022Quote: ... as well as respective inputs (20 µl of extracts corresponding to 50 µg of total proteins) were separated using SDS PAGE electrophoresis and transferred onto nitrocellulose membrane by electro-blotting (SemiDry, BioRad). Western blots were probed with rabbit anti-GFP (1:5000 ...
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bioRxiv - Cancer Biology 2022Quote: ... and 2 µl of the lysates were subject to 1 ml of 0.2x Bradford protein dye reagent (Bio-Rad #5000006) for protein quantification ...
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bioRxiv - Microbiology 2022Quote: ... 7.5 mg of proteins was diluted in ChIP buffer supplemented with 0.01% SDS and precleared for 1 h at 4 °C with 50 μl of SureBeads Protein A Magnetic Beads (BioRad) and 100 μg bovine serum albumin (BSA) ...
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bioRxiv - Immunology 2022Quote: ... The protein extracts were subjected to appropriate concentrations of SDS–PAGE for electrophoresis and transferred to PVDF membranes (Bio-Rad). Membranes were blocked with 5% bovine serum albumin (ThermoFisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... Total of 15-30µg protein were separated on a 4-12% Bis-Tris SDS PAGE and transferred using BioRad Turbo Blot (1704156, BioRad) onto PVDF membrane ...
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bioRxiv - Genetics 2022Quote: ... Proteins were separated by SDS-PAGE with 2-mercaptoethanol as a reducing agent using criterion sized 4-15% gradient tris-glycine for broad range of molecular weights greater than 30 kDa or 16.5% tris-tricine for high resolution of smaller proteins (Bio-Rad). Proteins were transferred to nitrocellulose membranes and probed with antibodies [commercially obtained except for ATF6 [63] ...
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bioRxiv - Cancer Biology 2022Quote: Protein separations were transferred onto a nitrocellulose membrane using the Trans-blot Turbo RTA transfer kit (Bio-rad, #170-4270) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: Proteins were resolved in 6-8-10 or 12% polyacrilammide gels and transferred to PVDF (Bio-Rad, Hercules, CA,USA) or nitrocellulose membranes (GE Heath Care ...
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bioRxiv - Biochemistry 2022Quote: Proteins were resolved by SDS/PAGE and transferred to nitrocellulose membranes using the Trans-Blot Turbo transfer system (Bio-Rad). Membranes were blocked with 5% BSA in Tris-buffered saline containing Tween 20 (TBS-T ...
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bioRxiv - Neuroscience 2022Quote: ... Aliquots of the lysates containing 10 mg of protein were denatured in Laemmli buffer and β-mercaptoethanol (Bio-Rad, USA) at 95°C for 5 min ...
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bioRxiv - Neuroscience 2022Quote: ... The protein concentration for each condition was measured directly from 1 µL aliquot of the sample using NanoDrop (record A280nm measurements and use the exact same protein sample buffer as blank) and the Bradford assay (Biorad). After incubation for different amounts of time (1 hour after dialysis or dilution to allow droplet maturation ...
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bioRxiv - Physiology 2022Quote: ... Lysates were boiled and 20-60μg of protein was loaded onto Mini-PROTEAN® TGX™ Precast Gels (Bio-Rad). SDS-PAGE gels ran for 50 minutes at 125 V ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The proteins were then transferred to a nitrocellulose membrane on a Trans-Blot® Turbo (Bio-Rad; Hercules, CA, USA). The membrane was blocked in a PBS solution containing 5% non-fat milk and 0.1% Triton X-100 for 1 h with orbital agitation ...
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bioRxiv - Microbiology 2022Quote: ... 10 μg of protein was separated by SDS-PAGE using 12% acrylamide Mini-protean TGX precast gels (Bio-Rad, USA). Gels were stained with Coomassie (Sigma-Aldrich ...