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Citations for Bio-Rad :
801 - 850 of 932 citations for Water RNase Free since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... 5-10μg of total proteins were separated on bis-tris polyacrylamide gradient gels (NuPAGE Novex 4-12% Bis-Tris Gel, Life tech, #NW04120BOX) or TGX stain-free gels (BioRad, #4568126) and transferred to PVDF membranes ...
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bioRxiv - Microbiology 2023Quote: ... 10% ß-mercaptoethanol) was used as the starting material for separation on 4–20 % Mini-PROTEAN TGX Stain-Free Protein Gels (Bio-Rad). The purified virus was loaded into the sample well and was run on the gel placed in a Mini-PROTEAN Tetra Vertical Electrophoresis Cell for 30 min at 200 V ...
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bioRxiv - Cell Biology 2023Quote: ... the lysates were mixed with an equal volume of 2X Laemmli sample buffer and incubated for 5 min at 95°C prior to separation by SDS PAGE on Criterion TGX stain-free 4-15% gradient gels (Bio-Rad). Following SDS-PAGE ...
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bioRxiv - Cell Biology 2023Quote: ... Band intensities were normalized to total protein in the sample quantified within each lane using Stain-Free Dye Imaging (Bio-Rad).
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Pushed to the edge: hundreds of Myosin 10s pack into filopodia and could cause traffic jams on actinbioRxiv - Cell Biology 2023Quote: ... All samples were boiled at 70°C for 10 min before loading onto a 4-20% Mini-PROTEAN TGX Stain-Free protein gel (BioRad, 4568095). The gel was run at 180 V for 45 min and imaged on a ChemiDoc ...
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bioRxiv - Microbiology 2023Quote: Proteins were separated by SDS-PAGE on 4-15% gradient Mini-Protean TGX Stain-free gels (cat. no. 4568083, Bio-Rad), transferred onto PVDF membranes (cat ...
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bioRxiv - Biochemistry 2023Quote: Intracellular and soluble-secreted proteins were Deoxycholate/Trichloroacetic acid– precipitated and separated by SDS-PAGE on gradient 4-20% PROTEAN TGX Stain-Free Gels (Bio-rad) and proteins were transferred to polyvinylidene difluoride membranes within Trans-Blot Turbo Transfer Packs (Bio-rad ...
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bioRxiv - Bioengineering 2023Quote: ... Equal amounts of the proteins (50 µg) were electrophoresed (125 volts, 1 h) on a 4-20% Mini protean TGX stain-free protein gel (Bio-Rad) and transferred onto nitrocellulose membranes (125 volts ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein was quantified by Qubit and 15ug protein was run on a 4-20% Mini-PROTEAN(R) TGX Stain-Free Protein gel (Bio-Rad). Protein was transferred to a low background fluorescence PVDF membrane (Millipore Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... and Precision Plus Protein Unstained Standards (#1610363) was resolved by SDS-PAGE using precast 4-20% MiniProtean TGX Stain Free Gels (Bio-Rad). The blot in Fig ...
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bioRxiv - Biochemistry 2023Quote: ... the clear supernatant was loaded on a 4–20% Mini-PROTEAN TGX Stain-Free Gel (Bio-Rad Laboratories, Inc., Hercules, USA) including PageRuler™ Plus Prestained Protein Ladder (Thermo Fisher Scientific Inc. ...
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bioRxiv - Neuroscience 2023Quote: ... Equal amounts of proteins (20–100μg as optimal for each antibody) for each sample was loaded into 4-15% Mini-PROTEAN TGX Stain-Free Protein Gels (BioRad; #4568084) for electrophoresis ...
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bioRxiv - Molecular Biology 2023Quote: ... and 0.1 vol of DTT 1M was added and the mixture heated at 95°C for 5 min before loading onto Any kD Mini-PROTEAN TGX Stain-Free protein gel (4568124, Bio-Rad) and migration in 1x Tris-Glycine-SDS buffer at 200V ...
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bioRxiv - Molecular Biology 2023Quote: ... resin with immunoprecipitated proteins were resuspended in SDS-loading buffer and electrophoresed on a 4-20% TGX stain-free gel (Bio-Rad) for 10 min ...
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bioRxiv - Microbiology 2023Quote: ... Samples (24 µg) were denatured by heating 5 minutes at 95°C and separated by Stain-Free SDS-PAGE gel (4568096, Bio-Rad). The gel was activated using one-minute setting (Bio-Rad Imager ...
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bioRxiv - Biophysics 2023Quote: ... Samples were heated at 95 °C for 5 minutes to denature and reduce the proteins and were then separated by electrophoresis through 4-15% Mini-PROTEAN TGX Stain-Free gel (Bio-Rad) with Tris/Glycine/SDS running buffer ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Proteins in cytosolic or nuclear extracts from mouse livers were resolved in sodium dodecyl sulphate-polyacrylamide gel electrophoresis using the mini-PROTEAN TGX Stain-Free Precast Gels from Bio-Rad. Primary antibodies were revealed with HRP-conjugated secondary antibodies (anti-rabbit IgG #7074 ...
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bioRxiv - Cell Biology 2023Quote: ... 10µg and 5 µg /sample) were resolved by SDS-PAGE on Mini-PROTEAN TGX Stain-Free Gels (Cat. # 4568086; Bio-Rad) and were run alongside a Page Ruler prestained protein ladder (Cat ...
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bioRxiv - Neuroscience 2023Quote: ... 10 µg of proteins in Laemmli buffer and protein standard were loaded on 4%-15% Criterion TGX Stain-Free protein gel in running buffer TGS 1XL(all from Bio-Rad) and transferred to a 0.2Lµm PVDF membrane with the Trans-Blot Turbo RTA Midi Transfer Kit (Bio-Rad) ...
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bioRxiv - Immunology 2024Quote: ... Equal amounts of pre-cleared lysate samples were separated in 8 – 16% Mini protean-TGX stain free protein gel (Bio-Rad) and the gel was transferred to a Trans-Blot Turbo Mini-PVDF membrane (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... Supernatants were mixed with reducing SDS sample buffer and incubated for 5 min at 95°C prior to fractionation by SDS-PAGE analysis on 4–15 % Mini-PROTEAN TGX Stain-Free Protein Gels (Bio-Rad). Western Blot analysis was performed as described previously (15).
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bioRxiv - Molecular Biology 2024Quote: ... Total protein content of cell free BALF was determined using the DC Protein Assay Kit (Cat # 5000111; BioRAD, Inc, Hercules CA) with bovine serum albumin as a standard according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: ... Membranes transferred from TGX Stain-Free™ gels were imaged and quantified for posterior normalization with a ChemiDoc XRS+ (Bio-Rad) using the Image Lab software (Bio-Rad) ...
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bioRxiv - Molecular Biology 2024Quote: ... a total of 20 µg whole cell lysate was resolved in a 10 % precast Mini-PROTEAN TGX Stain-Free gel (Bio-Rad). At the end of the run ...
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bioRxiv - Biochemistry 2024Quote: ... and 5 to 20 mM imidazole (wash-buffer) until the eluate was protein-free as judged by the lack of reaction with Bradford dye reagent (Bio-Rad). The calpain-3-titin/I81-I83 complex was eluted with 300 mM imidazole in wash-buffer ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were boiled for 5 min in Laemmli sample buffer before being separated at 200 V for 40 min using TGX stain-free precast gels (Bio-Rad). Stain-free protein gels were activated with UV light for 5 min and then transferred to PVDF membranes (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... and 2% β-mercaptoethanol) and separated on a 10% gel by SDS-PAGE (TGX Stain-Free™ FastCast™ Acrylamide Solutions; BIORAD) or 4-15% gel (Mini-PROTEAN® TGX Stain-Free™ ...
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bioRxiv - Biochemistry 2019Quote: ... Cells were lysed with 100 μL Laemmli buffer 2x and 20 μL of each sample (including -CL) was loaded in Criterion™ TGX Stain-Free™ Gels (4-20%, BioRad). SDS-PAGE and Western blots were used to reveal the proteins HuR ...
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bioRxiv - Immunology 2021Quote: ... Cytokine concentrations in cell free culture medium or serum samples were measured using the Bio-Plex Pro Assay System (Bio-Rad Laboratories) [23] ...
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bioRxiv - Molecular Biology 2020Quote: ... LC3B-I and LC3B-II protein levels were measured by densitometric analysis of Western blots and normalized to the amount of total protein as determined by densitometric analysis of stain-free gels using ImageLab™ software (Bio-Rad). Using the normalized values as determined previously by densitometric analysis ...
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bioRxiv - Neuroscience 2021Quote: A 30 μL aliquot of either medium or lysate for each sample was heated at 95 °C for 10 min and loaded on two separate SDS-PAGE gels (Any kD™ Mini-PROTEAN® TGX Stain-Free Protein Gels; BioRad). Proteins were electrophoretically transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples (n = 80 embryos / lane) were run on a precast SDS-PAGE gel (4-20% MP TGX Stain-Free Gel, Bio-Rad 4568093), with a Precision Plus Protein WesternC Stds ladder (Bio-Rad 1610376) ...
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bioRxiv - Cell Biology 2020Quote: ... a part of each sample was run on a 4-15% Criterion TGX Stain-Free Protein Gel (26 wells, Bio-Rad, #5678085) in 1x SDS running buffer at 200 V for 32 min ...
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bioRxiv - Neuroscience 2020Quote: ... 10 μg of protein per sample were loaded and separated using SDS-PAGE on 4-15% gradient stain-free tris-glycine gels (Mini Protean TGX, BioRad, Hercules, CA), transferred to low fluorescence PVDF membranes (45 μm ...
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bioRxiv - Cell Biology 2022Quote: ... The stain-free gel was UV-activated for 1 minute and imaged for total protein (BioRad Chemi Doc XR+, Hercules, CA, USA) with ImageLab software (BioRad Image Lab v6) ...
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bioRxiv - Immunology 2020Quote: ... Proteins were denaturated at 95°C for 5 minutes before separation on SDS-PAGE TGX 12% Stain-Free FastCast Acrylamide (Bio-Rad Laboratories). Proteins were then electro-transferred onto Trans Blot Turbo polyvinylidene fluoride membranes (Bio-Rad Laboratories) ...
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bioRxiv - Neuroscience 2021Quote: ... 10 μg of protein per sample were loaded and separated by SDS-PAGE on 4-15 % gradient stain-free tris-glycine gels (Mini Protean TGX, BioRad, Hercules, CA), transferred to low fluorescence PVDF membranes (45 μm ...
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bioRxiv - Immunology 2020Quote: ... Immunoblots were conducted using Mini-Protean TGX stain-free gels and Transfer-Blot Turbo RTA Transfer Kit reagents(Bio-Rad, Mississauga, Ontario). ABCF1 protein was probed with primary anti-ABCF1 antibody (HPA017578 ...
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bioRxiv - Bioengineering 2021Quote: ... 10 µg/lane of S was run on 4–20% Mini- PROTEAN® TGX Stain-Free™ Protein Gels (BioRad, Cat#4568081), transferred to nitrocellulose (Sigma ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes transferred from TGX Stain-Free™ gels were imaged and quantified for posterior normalization steps prior to blocking with a ChemiDoc XRS+ (Bio-Rad) using the Image Lab software (Bio-Rad).
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bioRxiv - Microbiology 2021Quote: Cell lysates were run under reducing conditions on an Any kD™ Mini-PROTEAN® TGX Stain-Free™ Protein Gel (BioRad) and imaged using a ChemiDoc imaging system (Biorad) ...
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bioRxiv - Microbiology 2021Quote: ... Protein extracts were subjected to SDS-PAGE for 90 min at 130 V at room temperature on 12% Mini-PROTEAN TGX Stain-Free gels (Bio-Rad #4568046). Samples were transferred to nitrocellulose membranes using a BioRad Trans-Blot Turbo system (“standard sd” protocol) ...
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bioRxiv - Physiology 2019Quote: ... 25 µg of protein extract per sample was separated on 12% acrylamide gels (#5678044, Criterion™ TGX Stain-Free™ Protein Gel, Biorad) and blotted on PVDF membranes (Immobilon-FL IPFL00010 ...
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bioRxiv - Immunology 2019Quote: ... The protein pellet was washed twice with methanol before boiling in 1X non-reducing loading buffer for 5 min and loading onto a 4-15% TGX stain-free gel (Bio-Rad 4568085). Following SDS-PAGE ...
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bioRxiv - Physiology 2019Quote: ... 1 cOmplete™ protease inhibitor cocktail EDTA free tablet) and protein concentrations determined using the DC protein assay (Bio-Rad, Hercules, CA). Oxygen Consumption Rate (OCR ...
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bioRxiv - Developmental Biology 2021Quote: ... Proteins were separated by SDS-PGE on a 4-15% Mini-PROTEAN® TGX Stain-Free™ gel (Bio-Rad #456-8084) and transferred with the Trans-Blot® Turbo™ RTA Transfer Kit (Bio-Rad #170-4275 ...
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bioRxiv - Molecular Biology 2021Quote: ... Equal volumes of leaf protein were separated by SDS-PAGE (Mini-PROTEAN® TGX stain-free™ precast gels; Bio-Rad system) and cys-OLE was detected by immunoblotting as described in Winichayakul et al. ...
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bioRxiv - Neuroscience 2020Quote: ... 10 μg of protein per sample were loaded and separated by SDS-PAGE on 4-15 % gradient stain-free tris-glycine gels (Mini Protean TGX, BioRad, Hercules, CA), transferred to low fluorescence PVDF membranes (45 μm ...
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bioRxiv - Immunology 2021Quote: ... For the three MuSK-specific (mAb MuSK1A, MuSK1B and MuSK3-28) and AChR-specific mAb 637 Mini-PROTEAN® TGX Stain-Free™ Precast Gels (Biorad) and Laemmli Sample Buffer (Biorad ...
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bioRxiv - Molecular Biology 2022Quote: ... The thawed samples were separated by SDS-PAGE using Mini-PROTEAN® TGX Stain-Free™ gels (usually 4-20%, Bio-Rad), and subsequently transferred to nitrocellulose membranes by a semi-dry blotting procedure as per manufacturer guidelines ...