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Citations for Bio-Rad :
801 - 850 of 860 citations for Tryptic Soy Agar TSA CRITERION Dehydrated Culture Media since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... culture supernatants samples from three biological replicates were injected on a 7.8 × 300 mm Aminex HPX-87H column (Biorad) combined with a 4.6 × 30 mm Cation H guard column (Biorad) ...
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bioRxiv - Neuroscience 2023Quote: ... the gold particles were brought to room temperature and delivered to slice cultures via a Helios Gene Gun (BioRad). Construct expression was confirmed by YFP epifluorescence.
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bioRxiv - Molecular Biology 2022Quote: ... The protein concentrations of culture medium and cell lysate were determined by the Bradford assay (Bio-Rad, Hercules, CA). Culture medium or cell lysate samples were resuspended in SDS sample buffer containing β-mercaptoethanol and incubated at 95°C for 5 min ...
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bioRxiv - Microbiology 2024Quote: ... spots or liquid cultures was performed using the ChemiDocTM MP Imaging System and the Image Lab software version 6.1.0 (Bio-Rad). Bright light acquisition was performed by using the “White Epi Illumination” setting combined with the “Standard Filter” ...
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bioRxiv - Cell Biology 2024Quote: ... we established co-cultures by plating specific numbers of different cell types measured by a cell counter (TC10, BioRad). After 3 hours ...
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bioRxiv - Microbiology 2020Quote: ... the remaining resin and media were mixed well and poured into a gravity flow column (Bio-rad, Hercules, CA, USA). The column was treated with 3 column volumes (CVs ...
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bioRxiv - Biophysics 2022Quote: Detergent removal and generation of proteoliposomes was achieved using polystyrene beads (Bio-Beads in SM-2 Adsorbent Media, BioRad, Inc.). The protocol was adapted and refined from 20 ...
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bioRxiv - Bioengineering 2023Quote: ... cell-seeded scaffolds were transferred into a new well and 200μL of fresh media containing 10% Alamar blue (cat. BUF012A Bio-Rad) was added to each scaffold ...
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bioRxiv - Microbiology 2022Quote: ... the base media was prepared without any of the metal components (magnesium, calcium, iron, and manganese) and treated with Chelex (BioRad) to remove contaminating metals ...
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bioRxiv - Cell Biology 2023Quote: ... Protein concentration in the conditioned media and lysates were determined by DC protein assay (Bio-Rad, Hercules CA, 500-011).
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bioRxiv - Microbiology 2021Quote: Strains were grown to mid-log cultures and RNA isolated as described above and reverse-transcribed into cDNA using iScript (Biorad). qRT-PCR was performed on a QuantStudio 3 PCR machine (Applied Biosystems ...
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bioRxiv - Genetics 2020Quote: ... LSK were plated at day 8 of culture and monitored for growth by counting live cells by Trypan blue exclusion using a TC20TM Automated Cell Couter (BIORAD) at the indicated time ...
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bioRxiv - Immunology 2021Quote: Concentration of 27 inflammation-related human proteins were measured in cell culture supernatants by using Human 27-plex BIORAD cytokine panel (Cat. No.171-A1112, BIORAD). Standard curves were generated for all 27 cytokine standards with eight 2-fold serial dilutions starting from 32 ng/ml ...
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bioRxiv - Microbiology 2023Quote: ... Supernatant fractions were normalized according to the OD600 of the bacterial culture at the time of harvest and samples were loaded on a 4-15% polyacrylamide gradient gel (BioRad), subjected to SDS-PAGE ...
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bioRxiv - Cell Biology 2023Quote: ... Additional antibodies used for the infected ALI cultures include: mouse anti-RSV Fusion protein (1:100, Cat# MCA490, Bio-Rad), rabbit anti-Cleaved Caspase-3 (Asp175 ...
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bioRxiv - Cancer Biology 2022Quote: The isolated sEVs and m/lEVs from 1.5 × 106 cells following 72 h of cell culture were lysed with 4X sample buffer (Bio-Rad) with β-mercaptoethanol (for detecting TSG101 and flotillin-1 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... were then generated using the piggyBac transposon system as described.12 Protein expression was induced with doxycycline and secreted fusion protein was separated from expression media using IgG Sepharose 6 Fast Flow resin (GE-Healthcare) in a 10 mL Poly-Prep chromatography column (Bio-Rad). Resin was washed with 50 column volumes of wash buffer (10 mM Tris pH 7.5 ...
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bioRxiv - Pathology 2021Quote: ... 0.10 M) at 50°C overnight followed by cleanup on 150 μL cation exchange media (AG50WX8, Bio-Rad, Hercules, CA, US) on top of C18 SPE columns (Strata C18-E ...
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bioRxiv - Neuroscience 2021Quote: ... cell lysates or cell media were subjected to SDS-PAGE using 4-20% gradient Tris-glycine gels (Bio-Rad; Hercules, CA) followed by transfer to Immobilon P membranes (EMD Millipore) ...
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bioRxiv - Biophysics 2024Quote: ... Growth media was exchanged after 24 hours and cells were checked for protein expression using a fluorescent cell imager (Bio-Rad). Cells were harvested after another 24 hours in cold PBS before purification ...
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bioRxiv - Cancer Biology 2024Quote: ... equipped with a 5-mL Foresight Nuvia HPQ anion-exchange column prepacked with CHT ceramic hydroxyapatite XT mixed-mode chromatography media (Bio-Rad) or 25-mL column in-house packed with the same CHT media ...
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bioRxiv - Cell Biology 2024Quote: Cell growth in liquid media was monitored in a 96-well flat-bottomed plate using a microplate reader (iMark, Bio-Rad). Cells were grown to the mid-log phase and then diluted to OD600 = 0.1 ...
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bioRxiv - Microbiology 2024Quote: ... Samples were mixed in nanodisc buffer (25 mM Napi, 100 mM NaCl, pH 7.0) with Bio-beads™ SM-2 Adsorbent Media (Bio-RAD) for cholate and detergent (DDM ...
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bioRxiv - Synthetic Biology 2021Quote: ... The culture supernatant was obtained by centrifugation and used with the Bio-Plex Pro Human Cytokine 8-plex Assay (Bio-rad) according to manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... when the bacterial culture was within the logarithmic phase of growth measured by SmartSpec 3000 spectrophotometer (Bio-Rad, Hercules, CA, USA), the suspension was centrifuged at 1500 g for 10 min to harvest the pellet ...
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bioRxiv - Cell Biology 2022Quote: Images of THP-1 wt and THP-1 RTN1A+ cells in cultures were taken with the ZOE fluorescence cell imager (Bio-Rad). and the numbers of cellular aggregates per 0.7 mm2 from 4 FOV have been quantified using ImageJ Fiji (Fig 5G) ...
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bioRxiv - Neuroscience 2023Quote: ... Immunoblotting of rodent cultures was detected by chemiluminescence with clarity Western ECL on ChemiDoc Touch system with Image Lab software (Bio-Rad) using a rabbit anti-GluK2/GluK3 (Merck-Millipore 04-921 ...
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bioRxiv - Microbiology 2022Quote: ... conditioned culture medium was collected after 24 h and assessed by Luminex assay (Bio-techne) and a Bioplex-200 machine (Bio-Rad). Bioplex manager 6.1 software was used to determine analyte concentrations.
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bioRxiv - Neuroscience 2023Quote: Seven DIV slice cultures were transfected with enhanced yellow fluorescent protein (eYFP) by particle-mediated gene transfer using a Helios Gene Gun (Bio-Rad), as described (Alonso et al. ...
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bioRxiv - Neuroscience 2023Quote: Cell cultures and animal tissues: 15 μg of protein extracts were loaded on 10% pre-cast gels (Bio-Rad Cat.# 4568034) and run for 1hr at 100V ...
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bioRxiv - Cell Biology 2022Quote: ... droplets were viewed on glass slides or in glass-bottom cell culture dishes for droplet and fluorescence imaging using ZOETM Fluorescent Cell Imager (BIO-RAD).
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bioRxiv - Microbiology 2022Quote: Cell pellets from 2 mL overnight cultures were used to create gel plugs using the CHEF Bacterial Genomic DNA Plug Kit (Bio-rad). DNA was digested with I-SceI ...
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bioRxiv - Microbiology 2023Quote: ... Anaerobic cultures were quickly aliquoted into a clear 96-well plate and then sealed thoroughly with an optical seal (Bio-Rad), Aerobic cultures were similarly added to the other half of the plate ...
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bioRxiv - Cancer Biology 2024Quote: Cells were lysed after culture and the RNA is extracted using the AurumTM Total RNA mini kit (BIO-RAD, 732-6820) following the manufacturer’s protocol and was resuspended in 40ul of nuclease-free water and stored at -80°C ...
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bioRxiv - Bioengineering 2022Quote: ... and biotinylated hFLO media were loaded into 16% SDS-PAGE gel and transferred onto 0.2 μm nitrocellulose membranes (1620150, Bio-Rad, CA, USA) through electro blotting ...
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bioRxiv - Microbiology 2020Quote: ... containing 2 g l-1 glucose and 1.32 mM K2HPO4 by treating prepared media with a universal chelator (Chelex® 100 Chelating Resin, Bio-Rad 1421253), filtering sterilizing away the Chelex resin ...
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bioRxiv - Microbiology 2023Quote: ... Tissues were rinsed in DBPS and moved to semi-solid media (6:4 ratio of 1% agarose (BIO-RAD, Hercules, CA, USA) in DPBS:DMEM with 10% FBS) ...
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bioRxiv - Cell Biology 2024Quote: ... We measured the cytokine and chemokine levels in the media using a Bio-Plex 200 as per the manufacturer’s instructions (Bio-Rad, Cat# M60000007A). Cytokines and chemokines measured included IL-2 ...
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Development of ketobenzothiazole-based peptidomimetic TMPRSS13 inhibitors with low nanomolar potencybioRxiv - Pharmacology and Toxicology 2024Quote: ... Equal quantities of proteins from lysates and 30 µl of concentrated media were diluted in Laemmli sample buffer (Bio-Rad Laboratories, 1610747) with 2.5 % β-mercaptoethanol and separated on 4-15 % SDS-page precast gels (Bio-Rad Laboratories ...
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bioRxiv - Immunology 2021Quote: ... Cytokine concentrations in cell free culture medium or serum samples were measured using the Bio-Plex Pro Assay System (Bio-Rad Laboratories) [23] ...
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bioRxiv - Microbiology 2020Quote: Two hundred µL of overnight bacterial cultures were transferred 5 mL of a 1:10 dilution of TSB medium (Bio-Rad, USA) supplemented with 1 mg mL-1 L-tryptophan and incubated at 30°C overnight (Gordon & Weber 1951) ...
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bioRxiv - Immunology 2020Quote: ... Multiplexed cytokine measurements on macrophage cell culture supernatants were performed using a custom Bio-Plex Express Human Cytokine kit (Bio-Rad 17004073) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Pro-inflammatory and pro-fibrotic mediators were measured in 50µl of cell-free tissue culture supernatants using a 37-Plex Bio-Plex Pro™ Human Inflammation Panel 1 (Bio-Rad #171AL001M) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: Whole-cell lysates of gonococci were harvested by pelleting cultures at a standardized optical density and resuspending cells in 2x Laemmli solubilizing buffer (BioRad, cat number) before storage at −20°C ...
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bioRxiv - Microbiology 2024Quote: ... trachomatis was detected by direct immunofluorescence staining of the coverslips with a Pathfinder Chlamydia Culture Confirmation System (Bio-Rad, Hercules, CA, USA). The infected cells from wells with no coverslips were collected in sucrose–phosphate–glutamate ...
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bioRxiv - Neuroscience 2022Quote: After 9-13 days in culture, organotypic hippocampal slices were transfected biolistically with gene gun (McAllister, 2000) using gold beads (Bio-Rad, 1.6 µm) coated with plasmids ...
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bioRxiv - Microbiology 2020Quote: Protein digestion was confirmed by running treated and untreated culture filtrate on precast polyacrylamide gel 4-20 % (Mini-PROTEAN® TGX Stain-Free™ Precast Gels, BIO-RAD) at 200V for 40 mins ...
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bioRxiv - Microbiology 2020Quote: ... and OD600 were measured from bacterial cultures diluted 1:10 using a Synergy H1 plate reader (Bio-Tek, Winooski, WI) and a Bio-Rad spectrophotometer (Bio-Rad, Hercules, CA), respectively ...
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bioRxiv - Cancer Biology 2023Quote: PDEC cytokine secretion was analyzed from cleared PDEC culture supernatants using Bio-Plex Pro Human Cytokine 27-plex assay kit (Bio-Rad, cat. M500KCAF0Y) and Bio-Plex 200 System (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: ... Both anti-IL-9 and the isotype antibody were purified from the culture supernatant of hybridoma cells using the Proteus protein purification spin kit (Bio-Rad, Hercules, CA). Anti-IL-13 is an IgG1 antibody (55 ...