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Citations for Bio-Rad :
801 - 850 of 4442 citations for 6 CHLORO 2 METHYLIMIDAZO 1 2 A PYRIDINE 3 CARBOXYLIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... the fractions that contained RTs were pooled and loaded onto a 2-ml Macro-Prep DEAE Resin (Bio-Rad, USA) column pre-equilibrated with buffer C (50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2019Quote: ... and the supernatants were purified with IBA Strep-Tactin sepharose beads (IBA, 2-1201-002) on Poly-Prep Chromatography Columns (731-1550, BioRad) with IBA’s buffer’s W (washing ...
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bioRxiv - Cell Biology 2020Quote: ... The embryos were mounted on a 2 % agarose pad and heat-shocked at 30°C for 1h (Thermocycler Bio-Rad). After 2h recovery at 20°C ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... with modified primer lengths that allowed all six loci to be used in combination in a single PCR in a DNA Engine Tetrad 2 thermal cycler from BioRad set at the following parameters ...
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bioRxiv - Biochemistry 2021Quote: ... 2.5×106 Jurkat T cells were electroporated with 2 μg minigene constructs using 220 V and 1,000 mA (Gene pulser X,Bio-Rad). After 24 h incubation ...
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bioRxiv - Immunology 2021Quote: For performing the digital droplet-PCR (ddPCR) we prepared the reaction mixture by combining a 2× ddPCR Mastermix (Bio-Rad), 20× primer ...
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bioRxiv - Biophysics 2021Quote: ... The solution was then transferred to a tube containing ∼30- 50 mg of Bio-Beads (Bio-Beads SM-2, BioRad) that were previously equilibrated in 20 mM Tris-HCl pH 7 ...
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bioRxiv - Microbiology 2020Quote: ... The PCR products were electrophoresed in a 2% agarose gel and visualized using a Bio-Rad gel system (Bio-Rad).
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bioRxiv - Immunology 2020Quote: ... Cathepsin B activity was determined using Magic Red™ (a cell-permeable and non-cytotoxic reagent that contains a cathepsin B target sequence peptide (RR)2 linked to a red (Cresyl Violet) fluorescent probe) and lysosomes were detected with acridine orange (Biorad), according to manufactures instructions.
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bioRxiv - Developmental Biology 2020Quote: ... 5 μL of PCR1 reaction (2 ng) were combined for the qPCR reaction using CFX 96 Touch Biorad qPCR thermocycler (Biorad). Δct was calculated by subtracting the mean Cq of each sgRNA to a non-targeting control ...
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bioRxiv - Genetics 2019Quote: ... Gels were transferred in buffer containing 20% methanol and 0.03% SDS for 2 hours at 4C at 28V onto 0.2μM nitrocellulose membranes (Bio-Rad, cat #1620097). Membranes were blocked in tris-buffered saline and Tween 20 (TBST ...
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bioRxiv - Biochemistry 2022Quote: ... The other two lanes were incubated with 1xTris/glycine/SDS buffer for 15 min and then incubated with 2 x Laemmli sample buffer (BioRad) for 15 min ...
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bioRxiv - Cell Biology 2022Quote: ... and the nuclear ACTIN locus (Supplementary Table 2) were independently amplified from 12.5 ng of total genomic DNA with ssoAdvanced SYBR Green Supermix (Bio-Rad) in a total volume of 10 μl with standard cycling condition suggested by Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: ... before solubilisation in 2% SDS and subsequent OD595 measurements were obtained using an iMark microplate reader (Bio-Rad, Laboratories, Inc.). The number of adherent cells was claculated by the confluence in each concentration of CB-839/UK-5099 at 72 hours ...
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bioRxiv - Bioengineering 2022Quote: RNA was extracted as described above, followed by RT-PCR using the Allplex SARS-CoV-2 assay (SeeGene, South-Korea) on a CFX96 thermocycler (Biorad), simultaneously detecting four different target genes ...
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Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunitbioRxiv - Biochemistry 2022Quote: ... The protein extract was solubilized in 2-D lysis buffer (30 mM Tris-HCl pH 8.8, containing 7 M urea, 2 M thiourea and 4% CHAPS. Protein concentration was measured using Bio-Rad protein assay method ...
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bioRxiv - Cell Biology 2022Quote: ... Approximately 75 islets were sandwiched in a chamber between two layers of Bio-Gel P-2 (Bio-Rad, Cat#1504118) bead solution (200 mg beads/ml in KRB buffer) ...
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bioRxiv - Plant Biology 2022Quote: ... Then 2 μl of 10 times diluted cDNA was used for CFX96 Touch Real-Time PCR Detection System (Bio-Rad) for Syber green based quantitative PCR analysis ...
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bioRxiv - Zoology 2022Quote: ... and folded by heating to 95°C for 2 min and slowly cooling down at 0.1 °C per second using a thermocycler (Bio-Rad). DsRNA concentrations were adjusted to 3 or 14 μg / μl using SpeedVac Concentrator (Thermo Scientific ...
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bioRxiv - Microbiology 2022Quote: ... The resulting cDNA was diluted 1:10 and 2 µL was used for qPCR amplification of SARS-CoV-2 spike using iTaq Universal SYBR Green Supermix according to the manufacturer’s protocol (Bio-Rad). Copy number was calculated in reference to a standard curve of known copy number (102 – 107 copies spike) ...
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bioRxiv - Synthetic Biology 2022Quote: ... the mixture was transferred to ice-cold electroporation cuvettes with a 2 mm gap (Bio-Rad Laboratories GmbH, Germany, #1652086). Electroporation was performed using the MicroPulser Electroporator (Bio-Rad Laboratories GmbH ...
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bioRxiv - Synthetic Biology 2023Quote: ... and incubated for 24 h in a thermal gradient using a DNA Engine Tetrad 2 Peltier Thermal Cycler (Bio-Rad) with the lid set to 50°C ...
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bioRxiv - Microbiology 2022Quote: ... competent cells were mixed with 500 ng of DNA in electroporation cuvettes (2 mm gap) and the reactions were electroporated (2.5 kV) using a MicroPulser electroporator (Bio-Rad). Electroporated cells were recovered in 1 mL LB (unless otherwise stated ...
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bioRxiv - Developmental Biology 2022Quote: ... Protein lysates in Laemmli buffer were separated by electrophoresis on 12% SDS-PAGE gels and transferred for 2 hours at 4°C on to a PVDF membrane (BioRad). After blocking for an hour in 5% dry milk ...
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bioRxiv - Genomics 2023Quote: ... Gels were run in 1X tris-acetate SDS running buffer for 2 hr and transferred to a 0.45 μm PVDF membrane using the Turbo Trans-Blot transfer system (Bio-Rad) for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... 1.0 µg purified plasmid DNA was added to 0.2 ml aliquot of the above-prepared fusobacterial cells in a pre-cooled corvette (0.1 cm electrode gap, Bio-Rad) and left on ice for 10 minutes ...
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bioRxiv - Microbiology 2023Quote: ... The was filtered through a 0.45 μm filter and His-MSI-2 was purified with HisTrapTM FF column (Cytiva) using the Econo Gradient Pump (BIORAD) according to the manufacturer’s protocol and overnight dialysis ...
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bioRxiv - Biophysics 2022Quote: Detergent removal and generation of proteoliposomes was achieved using polystyrene beads (Bio-Beads in SM-2 Adsorbent Media, BioRad, Inc.). The protocol was adapted and refined from 20 ...
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bioRxiv - Neuroscience 2023Quote: ... membranes were subsequently blocked for 2 h at room temperature with 5% non-fat milk (Bio-Rad, Hercules, CA, USA) or 5% bovine serum albumin (BSA ...
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bioRxiv - Systems Biology 2023Quote: ... and total protein abundance was normalized to 2 μg using the Detergentcompatible Protein Assay (Biorad, Hercules, CA, USA, Cat. # 5000111). 2 μg α-RelA antibody (Cell Signaling ...
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bioRxiv - Microbiology 2023Quote: ... 1.0 mg purified plasmid DNA was added to 0.2 ml aliquot of the above-prepared fusobacterial cells in a pre-cooled corvette (0.1 cm electrode gap, Bio-Rad) and left on ice for 10 minutes ...
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bioRxiv - Genomics 2022Quote: ... Proteins were transferred to a 0.2 µm or a low-fluorescence 0.45 µm polyvinylidene difluoride (PVDF) transfer membrane using a Trans-Blot Turbo (Bio-Rad). Following antibody incubation ...
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bioRxiv - Genomics 2023Quote: ... Gels were run in 1X tris-acetate SDS running buffer for 2 hour and transferred to a 0.45 μm PVDF membrane using the Turbo Trans-Blot transfer system (Bio-Rad) for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA was generated from ∼2 ng of total RNA from each sample using iScript Reverse Transcription Supermix for qRT-PCR (170-8891; BioRad) according to manufacturer’s protocols ...
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bioRxiv - Cell Biology 2023Quote: Whole-cell lysates for immunoblotting were prepared by dissolving cells in Laemmli Sample Buffer containing 5% 2-mercaptoethanol (Bio-Rad). Protein samples were loaded on 4-15% polyacrylamide gels (BioRad ...
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bioRxiv - Microbiology 2023Quote: ... 390 μl of cells were mixed with 10 μg of in vitro transcribed RNA and added to a 2 mm electroporation cuvette (BioRad). BHK-21 cells were electroporated with 1 pulse at 1.2 kV ...
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bioRxiv - Microbiology 2023Quote: ... 0.2 μM of each primer and 2 μl of template cDNA on a CFX Connect Real-Time System (Bio-Rad). Duplicates of samples and plasmid standards were amplified by using the following protocol ...
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bioRxiv - Microbiology 2023Quote: ... 10 min), centrifuged (1,000 x g, 2 min) and loaded on 10% or 4–20% SDS-PAGE gel (Bio-Rad). Following electrophoresis (60 min ...
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bioRxiv - Cell Biology 2023Quote: ... then the pellet was resuspended in 212 µL of Laemmli sample buffer with 2-mercaptoethanol added (Bio-Rad, Hercules, CA). To neutralize the residual TCA ...
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bioRxiv - Microbiology 2023Quote: ... Relative expression was determined using the 2−ΔΔCT method129 as part of the CFX Maestro for Mac 1.0 software package (Bio-Rad).
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bioRxiv - Microbiology 2023Quote: ... The mixture was allowed to stand at room temperature in the dark for 30 min before the detergent was removed by successive addition of BioBeads SM-2 (BioRad). Nanodiscs were concentrated using Vivaspin® 500 centrifugal filters (10 kDa MWCO PES membrane).
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bioRxiv - Microbiology 2023Quote: ... DD-HA-BFD2Δeif1.2 were subjected to electrophoresis using 10% polyacrylamide SDS-PAGE gels and transferred onto 0.45-µm nitrocellulose membranes (Bio-Rad) with wet/tank transfer system (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Cell pellets were resuspended by addition of ice-cold PBS supplemented with 2% FBS and counted using a TC-20 cell counter (BioRad). 2.5-5x105 cells were harvested ...
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bioRxiv - Neuroscience 2024Quote: ... The samples (2 μg) were loaded onto a 15% SDS-PAGE gel and transferred to PVDF membranes (0.2 μm, Bio-Rad). After blocking with 5% BSA in TBST at room temperature for 1 hour ...
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bioRxiv - Immunology 2024Quote: ... mixed with 25 μl 1.5 mM CaCl2 and 42 μl ddH2O using 2 mm gap cuvettes with 3.5 msec/500 volt in BIORAD Gene Pulser Xcell Electroporation System (BioRad, USA). Transfected cells were immediately transferred into 5 mL prewarmed medium in 25 cm2 flasks and left to recover overnight at 26 °C ...
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bioRxiv - Microbiology 2023Quote: ... cells and plasmids in 10% glycerol were first heat-shocked at 56°C for 2 min followed by immediate electroporation in 0.1-cm cuvette (Bio-Rad) using electroporation program of 2.5 kV and time constant of 2.3-2.5 ms on the Bio-Rad Micropulser ...
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bioRxiv - Microbiology 2024Quote: ... 0.2 μM of each primer and 2 μl of template cDNA on a CFX Connect Real-Time System (Bio-Rad). Samples and plasmid standards were amplified using the following protocol ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were run on 2% TBE agarose gel and post-stained with ViSafe Red Gel Stain (Vivantis Technologies) and visualised using GelDoc (BioRad). The relative intensity of the bands was quantified using ImageJ ...
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bioRxiv - Biophysics 2023Quote: ... and yeast cell growth was monitored by measuring the OD600 at 2 h increments in a microplate reader (Benchmark Plus, BioRad). The remainder of the 10 ml cultures was used to assess Pgp expression by Western blot analysis of microsomal membrane preparations using the monoclonal C219 anti-Pgp antibody.
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bioRxiv - Genetics 2023Quote: ... China) using TBE (65 mM Tris-HCl, 27 mM boric acid, 1 mM EDTA, pH 9; Bio-Rad, Hercules, CA, USA) as the buffer ...