Labshake search
Citations for Bio-Rad :
8301 - 8350 of 9412 citations for Recombinant Human CPB1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2024Quote: ... 0.01% bromophenol blue) and denaturated 5 min at 95°C before protein separation by SDS–PAGE on 4-15% precast gels (Biorad).
-
bioRxiv - Microbiology 2024Quote: Equal amounts of Total Protein extracts were migrated on a 12.5% SDS-PAGE gel and transferred onto Immun-Blot PVDF blotting membrane (Bio-Rad). Transfer was performed with the Bio-Rad Transfer System (30 min ...
-
bioRxiv - Neuroscience 2024Quote: ... Samples were denatured for 10 minutes at 70°C and proteins were separated (55min, 200V) on a Midi 4-12% Bis-Tris gel (BioRad) in 1x MOPS buffer (BioRad ...
-
bioRxiv - Neuroscience 2024Quote: ... The adapted protein quantity per lane was loaded into 4-20% 1.5 mm Tris-glycine 15 well mini gels (Bio-Rad) with 1X Laemmli sample buffer ...
-
bioRxiv - Neuroscience 2024Quote: ... Equal amounts of protein were resolved in SDS-polyacrylamide gels and electrophoretically transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA) at 4°C ...
-
bioRxiv - Neuroscience 2024Quote: Samples (8–25 μg protein extract) were run on 8–10% acrylamide gel at 100 Volt in Running Buffer (1X Tris–Glycine-SDS, Biorad) together with Precision Plus Protein Dual Color Standards (Biorad) ...
-
bioRxiv - Neuroscience 2024Quote: ... 10-20 μg protein per sample were loaded onto SDS-page gels for protein separation and then transferred onto nitrocellulose or methanol-activated PVDF membranes via full-wet transfer assay (BioRad) or semi-wet transfer ...
-
bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred to a PVDF membrane using the Bio-Rad Turbo Transblot System and transfer kit (Bio-Rad Laboratories) and fixed in methanol prior to blocking in 5% non-fat milk in TNT buffer (50 mM Tris pH 8.0 ...
-
bioRxiv - Microbiology 2024Quote: Protein samples were incubated at 95°C for 10 minutes before being resolved on TGX Stain-free gels (Bio-Rad). The proteins were transferred onto 0.2 μm nitrocellulose membranes using Trans-Blot Turbo Transfer (Bio-Rad) ...
-
bioRxiv - Immunology 2023Quote: ... Buffer containing eluted protein was filtered through a 0.22-μm spin filter and directly loaded on the Enrich SEC650 column (Bio-Rad) for SEC ...
-
bioRxiv - Biophysics 2023Quote: ... proteins were transferred to PVDF membranes using a Trans-Blot Turbo RTA Mini 0.2 μm PVDF Transfer Kit (Bio-Rad) according to the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2024Quote: ... Protein separations were transferred onto a nitrocellulose membrane using the Trans-blot Turbo RTA transfer kit (Bio-rad, #170-4270) according to the manufacturer’s protocol ...
-
bioRxiv - Bioengineering 2024Quote: ... Each column was calibrated using 660 kDa to 1.35 kDa molecular weight protein standards (thyroglobulin, IgG, ovalbumin, myoglobin, and vitamin B12) (Bio-Rad). Following peak analysis by SDS-PAGE ...
-
bioRxiv - Neuroscience 2024Quote: ... Stain-free protein gels were activated with UV light for 5 min and then transferred to PVDF membranes (Bio-Rad) using a turbo transfer system (Bio-Rad) ...
-
bioRxiv - Molecular Biology 2024Quote: ... and then proteins were transferred to a nitrocellulose membrane using a wet Trans-Blot system (Bio-Rad, Hercules, CA, USA). The primary antibodies used were anti-slow MyHC (Sigma ...
-
bioRxiv - Microbiology 2024Quote: ... 7.5 mg of proteins was diluted in ChIP buffer supplemented with 0.01% SDS and precleared for 1 hr at 4°C with 50 μl of SureBeads Protein A Magnetic Beads (BioRad) and 100 μg bovine serum albumin (BSA) ...
-
bioRxiv - Physiology 2023Quote: ... Protein extracts were denatured at 95 °C for 10 min and separated on 10% SDS-PAGE gels (1610156, Bio-Rad) at 80 V for ∼40 min followed by 110 V for ∼70 min ...
-
bioRxiv - Cell Biology 2024Quote: ... 30µg of total proteins were loaded onto each well of Mini-PROTEAN TGX Stain-Free Precast Gels (10%, Biorad, # 4568031), and migrated for 45 min at 120 Volts ...
-
bioRxiv - Cell Biology 2024Quote: ... proteins were resolved by electrophoresis through 12% agarose polyacrylamide gels and then transferred to PVDF membranes (Bio-Rad Cat #1620175) by electroblotting ...
-
bioRxiv - Microbiology 2024Quote: ... Proteins were then transferred to a nitrocellulose membrane (Cytiva) using the semi-dry Trans-Blot Turbo Transfer system (Bio-Rad). The membrane was blocked in 5% milk in TBS-T (tris-buffered saline ...
-
bioRxiv - Immunology 2024Quote: ... protein was transferred to a 0.45-μM nitrocellulose membrane (Cytiva) with a Transblot Turbo semi-dry transfer system (Bio-Rad). Membranes were blocked with 5% BSA and 0.1% Tween 20 (IB blocking buffer ...
-
bioRxiv - Molecular Biology 2023Quote: ... The column was washed with 50 mL of wash buffer and then transferred onto a Fast protein liquid chromatography system (BioRad). The column was further washed until the UV trace was flat ...
-
bioRxiv - Biochemistry 2024Quote: ... 20μg of total protein per fraction were collected and diluted with their respective solvents and 4X Laemmli buffer (Biorad, 1610747) to obtain aliquots of equal volume ...
-
bioRxiv - Bioengineering 2024Quote: ... The proteins were incubated for one hour at 65 and 70°C in a thermocycler with heated lid (T100, BioRad) Binding to D25 and MPE8 antibodies was measured afterwards following the protocol described above.
-
bioRxiv - Cancer Biology 2023Quote: ... Western blots were loaded at 2.5 to 50 ug total protein per lane in 4-20% gradient pre-cast polyacrylamide gels (#3450033, Bio-Rad). Gels were also loaded with the Dual pre-stained protein standards (#1610374 ...
-
bioRxiv - Bioengineering 2023Quote: ... Protein transfer was performed on methanol-activated polyvinylidene fluoride (PVDF) membranes by Trans-Blot Turbo Transfer system (1704150; Bio-Rad). After transfer the membranes were blocked in 5% BSA in TBS with 0.1% Tween-20 for 1 hour at room temperature ...
-
A synthetic elastic protein as molecular prosthetic candidate to strengthen vascular wall elasticitybioRxiv - Cell Biology 2023Quote: ... Proteins were transferred onto poly(vinylidene fluoride) membrane using Trans-Blot Turbo Transfer System (Bio-Rad, Marnes-la-Coquette, France). After transfer ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Total protein (mg/mL) was determined for each sample using Bradford assay reagent (Catalogue # 5000002, Bio-Rad, Hercules, California, USA). Liver mitochondria (0.175 mg/mL ...
-
bioRxiv - Bioengineering 2024Quote: ... protein from gel was transferred to a PVDF membrane using the Trans-Blot Turbo Transfer System (Bio-Rad, cat.: 1704150).
-
bioRxiv - Molecular Biology 2023Quote: ... Separated proteins were transferred into a nitrocellulose membrane using the Trans-Blot Turbo transfer system (Bio-Rad, Hercules, CA, USA). The membrane was incubated with mouse polyclonal anti-GFP antibody (Roche ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Proteins of the parasite pellets were extracted using lysis buffer (1 mM DTT, 2X Laemmli buffer (Bio-rad, 161-0737) and protease inhibitor cocktail (Roche ...
-
bioRxiv - Physiology 2022Quote: ... Protein lysates were combined with Laemmli buffer and separated on a 4-15% Mini-PROTEAN TGC precast gel (Bio-Rad) by electrophoresis ...
-
bioRxiv - Neuroscience 2023Quote: ... Proteins were separated by SDS-PAGE then transferred to nitrocellulose membrane at 4°C (0.45 μm pore size, Bio-Rad). Membranes were blocked overnight at 4° in Intercept tris-buffered saline (TBS ...
-
bioRxiv - Microbiology 2023Quote: ... The protein concentration of crude cell extracts was measured using the Bradford method with BSA as a standard (Bio-Rad). The reaction mixture contains 8 μg total proteins and 4 μg nitrocefin (Calbiochem ...
-
bioRxiv - Cell Biology 2023Quote: ... Then the proteins were transferred to a PVDF membrane using Trans-Blot Turbo Mini 0.2 µm PVDF Transfer Pack (BioRad, 1704156). The membranes were blocked in 5% non-fat milk in TBST for 1 h at room temperature ...
-
bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were analyzed by SDS-PAGE and purities determined by densitometry of Coomassie Blue-stained gels (Image Lab Software, BioRad). Protein batches used in this work were ≥ 90% pure.
-
bioRxiv - Cancer Biology 2023Quote: ... Protein samples were allowed to migrated on 4-20% Mini-PROTEAN® TGX™ Precast Gels (Bio-Rad Cat. 4561094) and then transferred on nitrocellulose membranes (Bio-Rad Cat ...
-
bioRxiv - Neuroscience 2023Quote: ... Samples containing 20 µg of protein were separated on 4-20% Tris-Glycine SDS-PAGE gel (Bio-Rad, CA, USA) and transferred to PVDF membrane using Trans-Blot Turbo transfer system and transfer packs (Bio-Rad ...
-
bioRxiv - Neuroscience 2023Quote: ... The protein sample was denatured and run on 4-15% Mini-PROTEAN TGX Precast Protein Gels as per the manufacturer’s instructions (BIO-RAD). Blots were probed overnight at 4°C with antibodies recognizing GAD67 (1:1000 ...
-
bioRxiv - Plant Biology 2023Quote: ... Proteins were visualized by Coomassie blue staining and ATG3 monomer and dimer were quantified using the ImageLab software (Bio-Rad) and the sample treated with 20 mM DTTred and 0 mM DTTox (Eh= -∞ ...
-
bioRxiv - Plant Biology 2023Quote: ... or the proteins were transferred onto a nitrocellulose membrane using Mini Trans-Blot® cell (Bio-Rad, Hercules, CA, USA). The transfer was performed in 1x transfer buffer at 250 mA for 1 h at 4 °C under constant stirring ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The cleaved proteins were incubated with Ni-NTA beads for revers his-purification and cleaved proteins were collected in the flow through and visualized on SDS-PAGE (Mini Protean Tris-Tricine Precast gels, BioRad).
-
bioRxiv - Plant Biology 2023Quote: ... proteins separated in SDS-PAGE gels were transferred to a PVDF membrane using Trans-Blot Turbo Transfer System (Bio-Rad). For anti-His and anti-GFP blots ...
-
bioRxiv - Neuroscience 2022Quote: ... membranes were again washed three times with TTBS buffer and protein bands were visualized using the ChemiDoc Imaging System (BioRad) and quantified with Image Lab software (BioRad).
-
bioRxiv - Cell Biology 2023Quote: ... proteins were separated by SDS-PAGE and transferred onto nitrocellulose membrane using a Trans-blot Turbo system (Bio-Rad, 1704159). All Western blots were revealed using ECL (GE Healthcare ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... and protein concentrations were equalized prior to boiling in the recommended sample buffers supplemented with 2.5% beta-mercaptoethanol (Bio-Rad). SDS-PAGE electrophoresis was performed using 4–12% gradient bis-tris gels (Invitrogen) ...
-
bioRxiv - Cell Biology 2023Quote: ... 5uL of protein sample was loaded onto a 4-15% Mini-PROTEAN TGX Precast polyacrylamide gel from Bio-Rad (4561086). Protein samples were resolved and transferred to a PVDF membrane using the Trans-Blot STurbo Transfer System ...
-
bioRxiv - Cell Biology 2023Quote: ... The cells were spun down and the supernatant was used to measure protein concentration by using a Bradford Assay (BioRad). Equal protein concentrations were loaded onto a NuPAGE 4-12% Bis-Tris gels (Invitrogen) ...
-
bioRxiv - Cell Biology 2023Quote: ... membranes were stained with Ponceau S and total proteins were visualized using the ChemiDoc™ MP imaging system (Bio-Rad). To detect proteins of interest ...
-
bioRxiv - Molecular Biology 2023Quote: ... Obtained samples were denatured in a 1xLaemmli buffer at 95°C for 10 min and proteins separated using electrophoresis on a 4-15% mini-protean-TGX-precast-gel (BioRad). Proteins were then transferred on nitrocellulose membranes by a semi-dry method using transblot turbo transfer system (BioRad) ...