Labshake search
Citations for Bio-Rad :
8201 - 8250 of 10000+ citations for Hepatitis E ORF2 protein mouse Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Physiology 2023Quote: ... HRP-conjugated secondary antibodies were applied (Immun-Star Goat Anti-Mouse, 1:20,000, 1705047 and Immun-Star Goat Anti-Rabbit, 1:20,000, 1705046, Biorad), and then activated with Clarity Max western ECL (Bio-Rad) ...
-
bioRxiv - Microbiology 2023Quote: ... and then transferred to TBST supplemented with rabbit anti-mouse IgG antibody conjugated to horseradish peroxidase (Bio-Rad) at a 1:15000 dilution for 1h at 25°C ...
-
bioRxiv - Immunology 2023Quote: ... blots were incubated with horseradish peroxidase-conjugated secondary antibodies (goat anti-mouse or goat anti-rabbit) (Bio-Rad) at a 1:2,000 dilution in Tris-buffered saline with Tween 20 (TBST ...
-
bioRxiv - Microbiology 2023Quote: ... Membranes were incubated in 1:5,000 goat anti-mouse IgG (H+L)-HRP (Bio-Rad, catalog number 1706516) in 6% milk in PBS-T ...
-
bioRxiv - Immunology 2023Quote: ... Homogenates were filtered (0.22 µm) twice and analyzed by BioPlex-Pro Mouse Cytokine 23-Plex Immunoassay (Bio-Rad). Lung homogenates were quantified for IL-1α ...
-
bioRxiv - Neuroscience 2024Quote: ... Membranes were incubated overnight with the following primary antibodies: αWIPI2 1:3000 (mouse, Bio-Rad, Hercules, CA, MCA5780GA), αWIPI4/WDR45 1:2000 (rabbit ...
-
bioRxiv - Biochemistry 2024Quote: Quantum™ Simply Cellular® Mouse IgG beads were obtained from Bio-Rad (#FCSC815; lot #15515; Hercules, CA). The initial evaluation utilized final Ab35-AF647 concentrations of 5 or 10 µg/mL exactly following the vendor product information with all procedures done on ice or at 4°C ...
-
bioRxiv - Cancer Biology 2021Quote: ... The proteins were then transferred to a nitrocellulose membrane using the Trans-Blot Turbo Transfer System (Bio-Rad Laboratories). To enhance detection of HIF1α ...
-
bioRxiv - Cancer Biology 2021Quote: ... 20 μg of protein for each cell lysate was run on a 4-15% SDS gel (Bio-Rad – 4561083DC) and electrotransferred onto a PVDF membrane ...
-
bioRxiv - Cell Biology 2020Quote: ... the same samples were run in parallel in a Mini-PROTEAN® TGX 4-15% Precast protein gels (BioRad). For mass spectrometry assay ...
-
bioRxiv - Cell Biology 2020Quote: 10-40 µg of total protein lysate were separated by SDS-PAGE on 8% or 10% polyacrylamide minigels (BioRad) and transferred onto a nitrocellulose membrane (GE healthcare ...
-
bioRxiv - Cell Biology 2020Quote: ... supernatant fractions were subjected to immunoprecipitation with anti-Pex14 antibody immobilized on SureBeads Protein G magnetic beads (Bio-Rad) for 3 h at 4°C with rotation ...
-
bioRxiv - Cell Biology 2020Quote: ... Proteins were transferred to nitrocellulose membranes using the Trans-Blot® Turbo™ system (Bio-Rad Laboratories, Hercules, CA) and blocked with Western Blocking Reagent (Roche ...
-
bioRxiv - Molecular Biology 2021Quote: ... 25 μg of protein was then fractionated on Tris–glycine SDS-PAGE gradient (4–15% acrylamide) gels (BioRad; #3450123), transferred onto nitrocellulose membranes (Millipore) ...
-
bioRxiv - Molecular Biology 2021Quote: Crude extracts of the given strains were separated on 4-20% Criterion TGX precast midi protein gels (BioRad #5671094). The gel was washed in fixing solution (40% Ethanol 10% Acetic Acid ...
-
bioRxiv - Molecular Biology 2021Quote: ... The crude extracts were separate by SDS PAGE on 4-20% Criterion TGX precast midi protein gels (BioRad #5671094) and transferred to a 0.2 μm nitrocellulose membrane (BioRad #1620112 ...
-
bioRxiv - Biochemistry 2022Quote: ... 1:10 000 dilution for N-terminal Parkin Ab) was incubated one hour at RT and protein visualized using ClarityTM Western ECL Substrate (Bio-Rad).
-
bioRxiv - Cell Biology 2022Quote: ... The samples containing 20 µg of protein were mixed with 4× Laemmli loading buffer (Bio-Rad, Hercules, CA, USA). Subsequently ...
-
bioRxiv - Immunology 2022Quote: ... 50 µg of protein per sample were separated on a 4-15% MP TGX precast cassette (Bio-Rad, 4561083) at 100V for 1 hour 25 minutes before being transferred to a PVDF membrane ...
-
bioRxiv - Cell Biology 2022Quote: ... 30 μg of proteins were loaded and resolved by SDS-PAGE electrophoresis and transferred to nitrocellulose membranes (Bio-Rad). Membranes were blocked with TBS Tween / Milk 5% for 1 hour and incubated at 4°C with primary antibodies overnight ...
-
bioRxiv - Biochemistry 2022Quote: ... Before the protein transfer was carried out 45 min at 25 V using a Semi Dry Blotter (Bio-Rad), the transfer membrane was pre-incubated 5 min in methanol ...
-
bioRxiv - Biochemistry 2022Quote: ... The protein bands were treated by ECL western blotting detection reagents and visualized by ChemiDoc imaging system (Bio-Rad). Densitometry of the band intensity was further determined via Image J software.
-
bioRxiv - Physiology 2020Quote: ... Overall protein concentration (mg/ml) was measured according to the Bradford method (Bradford, 1976) using BioRad stock (BioRad, Finland) diluted with dH2O (1:5 ...
-
bioRxiv - Neuroscience 2020Quote: ... Equal amounts of proteins were loaded and separated by electrophoresis in a 4-15% gradient polyacrylamide gel (BioRad #1653320) or NuPAGE 4-12% Bis-Tris gel (Invitrogen #NP0322) ...
-
bioRxiv - Molecular Biology 2021Quote: Proteins were separated by SDS-PAGE using a Mini-PROTEAN TGX Gel (4-15% or 8-16%, Bio-Rad Laboratories ...
-
bioRxiv - Molecular Biology 2020Quote: ... the separated proteins were transferred onto PVDF membranes for 30 min using BioRad Trans-Blot Turbo system (Bio-Rad) at 110V ...
-
bioRxiv - Neuroscience 2021Quote: ... for high-sensitivity chemiluminescent protein detection with UVItec gel analysis systems or using enhanced chemiluminescence (ECL) reagents (Bio-Rad) with autoradiography film (Kodak Scientific Imaging Film ...
-
bioRxiv - Molecular Biology 2021Quote: ... Using a Bio- Rad Trans-Blot semidry transfer system proteins were transferred to a nitrocellulose membrane (#1704159, Bio-Rad) and blocked for 1 hour (PBS ...
-
bioRxiv - Cell Biology 2022Quote: ... 80 μg of total protein was separated by SDS-PAGE on a 4-15% Criterion TGX Gel (Bio-Rad) and transferred to a nitrocellulose membrane using the Trans-Blot Turbo system (Bio-Rad) ...
-
bioRxiv - Cell Biology 2022Quote: ... 30–50 μg of protein was resolved in 4-20% TGX gel (Bio-Rad Laboratories, Inc., Hercules, CA, USA) under reducing conditions and transferred onto a nitrocellulose membrane (Bio-Rad Laboratories) ...
-
bioRxiv - Cell Biology 2022Quote: ... sonicated and protein content was analysed by electrophoresis on TGX Stain-free pre-cast SDS-polyacrylamide gel (Bio-rad). Proteins were then transferred on PVDF membranes (GE Healthcare ...
-
bioRxiv - Molecular Biology 2021Quote: ... The proteins were transferred onto a nitrocellulose membrane using Trans-Blot Turbo Transfer System (Bio-Rad, cat. No. 1704158). StrepII-tagged proteins (RBBP6 ...
-
bioRxiv - Molecular Biology 2021Quote: ... The same amounts of protein extracts were separated on the 4%-20% Mini-Protean TGX gel system (Bio-Rad) and transferred to PVDF (0.45 μm pore size ...
-
bioRxiv - Physiology 2019Quote: ... 8 µg of total protein was separated by electrophoresis on a precast 4-15% TGX gradient gel (Bio-Rad) and transferred to a 0.45 µm nitrocellulose membrane ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... The detection of the proteins on the membrane was performed using the Clarity Western ECL Substrate kit (Bio-rad). The signals were captured using the Alliance Mini (UVITEC Cambridge ...
-
bioRxiv - Molecular Biology 2019Quote: Proteins were separated by SDS–polyacrylamide gel electrophoresis (PAGE) and transferred to a 0.2 μm nitrocellulose membrane (Bio-Rad). The membrane was blocked with 5% milk in PBX (0.05 % Triton X-100 in PBS ...
-
bioRxiv - Neuroscience 2019Quote: ... “any kD” (to quantify GDNF protein) or 7% (to quantify CBP) precast polyacrylamide gel (Bio-rad, Hercules, Cal, USA) and transferred to a polyvinylidene difluoride membrane (PVDF ...
-
bioRxiv - Immunology 2019Quote: ... total proteins were quantified in paw extracts using the Bicinchoninic acid technique (BCA) and 75-100µg of proteins were loaded on 12% polyacrylamide gels (Biorad). The migration step was followed by a liquid transfer on PVDF membranes (100V 40min ...
-
bioRxiv - Neuroscience 2019Quote: ... Lysates were cleared by centrifugation at 20.000g for 10min and quantified with the DC Protein assay Kit (Bio-Rad). 25 µg of the lysates were then separated on NuPAGE 4-12% Bis-Tris gels (Invitrogen ...
-
bioRxiv - Neuroscience 2019Quote: ... ~5 μg of protein per lane was separated on 4-15% TGX gels (Bio-Rad Laboratories, Hercules, CA, USA) at 200V for 40 minutes in tris-glycine running buffer (Bio-Rad) ...
-
bioRxiv - Biophysics 2019Quote: ... The retention time of both oligomer types was compared with a set of molecular weight protein standards (Bio-Rad) consisting of bovine serum albumin (66.5 kDa) ...
-
bioRxiv - Microbiology 2019Quote: ... Separated proteins were transferred onto a nitrocellulose membrane using the Trans-Blot® Turbo™ transfer system (Bio-Rad). The membrane was incubated with mouse polyclonal anti-GFP antibody (Roche ...
-
bioRxiv - Systems Biology 2020Quote: Whole cell fractions were isolated using RIPA buffer and protein concentrations were determined using a Bradford Assay (Bio-Rad). 20µg whole cell lysate were separated on 8-12% SDS-PAGE gels (depending on the molecular weight of the target proteins) ...
-
bioRxiv - Molecular Biology 2019Quote: ... Protein for immunoblotting was eluted from ten percent of beads by boiling in 2X Laemmli sample buffer (Bio-Rad). RNA was extracted from ninety percent of beads using TRIzol reagent (Thermo Fisher) ...
-
bioRxiv - Plant Biology 2019Quote: ... Total proteins (40 µg) were separated using SDS-PAGE (10 % acrylamide) and then blotted onto nitrocellulose membranes (1620112, Biorad). Jas9-VENUS and ACTIN were detected using mouse monoclonal antibodies anti-GFP 1:1,000 (11814460001 ...
-
bioRxiv - Animal Behavior and Cognition 2019Quote: ... A total of 10 µg protein/well was separated by electrophoresis on 12% Mini-PROTEAN TGX gels (Bio-Rad). Proteins were transferred to Immobilon-FL PVDF membranes (Millipore ...
-
bioRxiv - Immunology 2019Quote: ... For blotting the SDS-PAGE separated proteins were transferred to a PVDF membrane (Amersham Hybond P0.2 PVDF, 55 GE) by semi-wet transfer (BioRad) and blocked for 1 h with 2% milk ...
-
bioRxiv - Plant Biology 2019Quote: ... SDS-PAGE and protein transfer to nitrocellulose membranes were performed with a Trans-Blot Turbo Transfer System (BioRad, USA) according to the manufacturer’s protocol ...
-
bioRxiv - Synthetic Biology 2020Quote: ... protein samples were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to nitrocellulose membranes (Bio-Rad, 1620115). The membranes were blocked in Tris-buffered saline-Tween 20 containing 5% nonfat milk at room temperature for 1 h ...
-
bioRxiv - Bioengineering 2019Quote: ... and analysed for protein content using 12% SDS-PAGE gels (Mini-PROTEAN TGX Stain-Free Precast Gels, Bio-Rad). Protein gels were imaged using a BioRad Gel Doc EZ Imager and relative protein band intensity was determined using the BioRad ImageLab software.