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Citations for Bio-Rad :
8201 - 8250 of 10000+ citations for Cow Rho Associated Protein Kinase 1 ROCK1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... The protein-dye conjugate was purified by 3 rounds of size exclusion chromatography (Bio-Spin 6 column, BioRad, CA) and then aliquoted in 5 μL portions and stored at -80C until required.
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bioRxiv - Molecular Biology 2022Quote: ... under reducing conditions and proteins transferred to nitrocellulose membranes using a semidry Trans-Blot Turbo Transfer System (Bio-Rad). Antibody reactions were carried out in 1% skimmed milk in PBS with 0.1% Tween-20 and washed in PBS with 0.1% Tween-20 ...
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bioRxiv - Neuroscience 2022Quote: ... Lysates were then centrifuged at 12,000 rpm at 4°C for 20min and total protein extracts were boiled in Laemmli sample buffer (BioRad), separated by SDS-PAGE under reducing conditions and transferred to PVDF membranes (Millipore) ...
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bioRxiv - Plant Biology 2022Quote: ... proteins were electrophoretically transferred onto a polyvinylidene fluoride (PVDF) membrane using Semidry Trans-Blot apparatus (Bio-Rad, Hercules, CA) at 20 V for 20 min ...
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bioRxiv - Microbiology 2022Quote: ... in equal volume and then subjected to electrophoresis using the 7.5% Mini-Protean TGX Precast Protein Gels (Bio-RAD) in a non-denaturing buffer ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were separated using a gradient SDS PAGE gel (8-12%) and a Mini-Protean 3 Cell (Bio-Rad) at 85 V ...
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bioRxiv - Microbiology 2022Quote: ... total protein extracts were subjected to SDS page electrophoresis using 4-20% polyacrylamide gradient gels (Bio-Rad, Watford, UK) and transferred to nitrocellulose membranes using a Trans-blot Turbo transfer system (Bio-Rad) ...
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bioRxiv - Biophysics 2022Quote: ... 10 µL of 12x and 120x diluted protein prep was then loaded into a 15% SDS-PAGE gel (BioRad) with BioRad Precision Plus unstained standard for comparison ...
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bioRxiv - Biochemistry 2022Quote: ... 40 μg of total protein was separated by SDS-PAGE and transferred to a PVDF membrane (Bio-Rad 1620177). Membranes were blocked for 1 h in 5% milk in 1X PBST (or 5% BSA in 1X PBST for western blots of immunoprecipitations ...
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bioRxiv - Microbiology 2022Quote: ... and 4 µg of protein was separated by SDS-PAGE using pre-cast TGX 4-15% gradient gels (BioRad). Protein was transferred to a 0.45 µm nitrocellulose membrane ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were transferred from the remainder of the gel to nitrocellulose membrane using the Trans-Blot Turbo (Bio-Rad). Membranes were incubated in a 5% milk in 1X Tris-buffered saline ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins where eluted from the beads by directly adding 20 µL of Laemli Sample Buffer buffer 2X (Biorad, #1610747). Samples where then processed for SDS-PAGE and Western blotting.
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bioRxiv - Biochemistry 2020Quote: ... Protein bands were quantified on a BioRad Chemidoc Touch Imaging System using Image Lab software version 6.0.1 (Bio-Rad).
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bioRxiv - Cell Biology 2019Quote: ... Data were normalized to GAPDH expression or total protein determined via UV treatment of TGX Stain-Free gels (Biorad) followed by detection on the transfer membrane.
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bioRxiv - Developmental Biology 2020Quote: ... Proteins were separated on a SDS-PAGE Mini-PROTEAN TGX 4-15% gradient gel (Bio-Rad, catalog #456-1086) along a 1:1 mix of Amersham ECL Rainbow Molecular Weight Markers (Amersham # 95040-114 ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were denatured and proteins separated in SDS-PAGE in Criterion™ TGX Stain-Free™ Gels (Bio-Rad), and transferred onto nitrocellulose membranes with the Trans-Blot® Turbo™ Blotting System (Bio-Rad).
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bioRxiv - Neuroscience 2020Quote: ... Fifteen μg of protein per sample were separated in 4-20% SDS-polyacrylamide gel (BIO-RAD mini-protean-TGX) before electrophoretic transfer onto a nitrocellulose blotting membrane (Amersham ...
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bioRxiv - Microbiology 2021Quote: ... the remaining interphase was dried completely before resuspending in Tris-buffered saline (50mM Tris-Cl, 150mM NaCl, pH 7.6) for protein quantification using a Bradford Assay (BioRad). Aqueous phase was evaporated under nitrogen flow and used for LC-MS ...
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Transcriptome analyses reveal tau isoform-driven changes in transposable element and gene expressionbioRxiv - Neuroscience 2021Quote: ... 12.1 ug of protein from each sample was loaded onto a 10% Tris-Glycine gel (Biorad, cat no. 4561034) and then transferred onto a PVDF membrane and blocked for one hour with 5% BSA in 0.1% Tween/1xTBS ...
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bioRxiv - Neuroscience 2021Quote: ... Both the soluble (40 µg of proteins) and the insoluble (4 µl) fractions were spotted on nitrocellulose membrane (BioRad). The near infrared fluorescence signal was acquired by scanning the membrane at the LiCor Odissey using the 800 nm filter and images were analyzed using the Fiji software.
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bioRxiv - Microbiology 2020Quote: ... and the images were analyzed and the signals quantitated after total cell protein normalization using the Image Lab 6.0.1 software (Bio-Rad).
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bioRxiv - Microbiology 2020Quote: ... and 150 ml Protein A Sepharose (GE, 17-5138-01) in a Poly-Prep Chromatography Column (BioRad, 731-1550). Fabs were generated by Papain cleavage and purified by the use of the Pierce Fab Preparation Kit according to the manufacturers’ instructions (Thermo Scientific - #44985) ...
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bioRxiv - Cell Biology 2020Quote: ... SDS-PAGE was made with 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels 10-well (BioRad, 4561094) with 30 μg of protein lysate for well and running was 70 V for 2 hours ...
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bioRxiv - Microbiology 2020Quote: ... and 30μg of total protein per sample was mixed with Laemmli buffer supplemented with 2-mercaptoethanol (10%) (Bio-rad) and boiled at 99°C for 10 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were electrophoresed on SDS-PAGE using 4-15% Mini-PROTEAN® TGX™ precast gel (Biorad#456-1085) and transferred onto a nitrocellulose membrane using Trans-Blot® Turbo™ Transfer Pack (Biorad#170-4159) ...
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bioRxiv - Molecular Biology 2020Quote: ... The immunoprecipitated proteins were eluted by heating at 100°C during 15 minutes in 4x Laemmli Sample Buffer (BIORAD). Immunoprecipitated protein complexes were subjected to Western blot analysis ...
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bioRxiv - Immunology 2020Quote: ... Protein (15–100 μg per lane) was separated in polyacrylamide gels containing SDS and transferred to Nitrocellulose membrane (Biorad) and probed with different primary antibodies according to the manufacturer’s protocols ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins (50 μg) were resolved on 12.5 % SDS-PAGE gels and via electrophoretic transfer system (Bio-Rad, München, Germany) bonds moved to polyvinylidene fluoride (PVDF ...
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bioRxiv - Biophysics 2021Quote: ... coli HU protein was monitored using 20% SDS-PAGE and the concentration was determined using the Lorry assay (Biorad). The purified E ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were separated by SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis) and transferred onto nitrocellulose membrane (Bio-Rad) using TransBlot Turbo (Bio-Rad ...
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bioRxiv - Genetics 2022Quote: ... Eight micrograms of total protein lysate were resolved on 4–15% Mini-PROTEAN TGX Stain-free gels (Bio-Rad) and transferred onto Immuno-Blot PVDF membranes (Bio-Rad) ...
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bioRxiv - Genetics 2022Quote: ... The separated protein samples were transferred to the PVDF membranes using a Mini Trans-Blot Electrophoretic Transfer Cell (Biorad) at 100V for 1h ...
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bioRxiv - Systems Biology 2022Quote: Samples with ∼30 μg of soluble total proteins were loaded on 4%-20% TGX stain-free precast gels (Biorad). These proteins were then separated by electrophoresis and transferred on PVDF membrane using TurboBlot (Biorad) ...
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bioRxiv - Immunology 2022Quote: ... 30 ug of Protein extracts were separated by 4-12% Bold NuPage (ThermoScientific) and transferred onto PVDF membranes (BioRad). After blocking with 3% albumin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... Equal amounts of protein were separated by SDS-PAGE and transferred to 0.2 μm nitrocellulose membranes (BioRad, Cat# 1620112). After blocking the membranes in 2% BSA in Tris buffered saline (TBS)-Tween for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Twenty (HA blot) or forty (SREBP1 blot) micrograms of protein were mixed with Laemmli sample loading buffer (Bio-Rad), heated to 37 °C for 10 min ...
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bioRxiv - Cancer Biology 2022Quote: ... Approximately 10μg of protein was loaded per sample on a 4-15% SDS-PAGE Stain-Free TGX precast gel (BioRad). Gels were run at 150V for approximately 60 minutes ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein samples were separated on the 4-15% Mini-PROTEAN® TGX™ precast SDS-PAGE gels (Bio-Rad) and then transferred to polyvinyl difluoride (PVDF ...
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bioRxiv - Microbiology 2019Quote: ... Protein lysates were separated in 10% SDS-PAGE gel (Transgen, Beijing, China) by Mini-PROTEAN tetra Cell system (BioRad) and transferred to PVDF membrane ...
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bioRxiv - Genetics 2020Quote: ... Whole cell lysate protein samples (20-50 µg) were resolved on Criterion 4-20% gradient denaturing gels (Bio-Rad), transferred to nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Plant Biology 2020Quote: ... This activity was then standardized to the quantity of total proteins present in the sample (Bradford assay, Bio-Rad). For inhibitor assays ...
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bioRxiv - Physiology 2020Quote: ... Twenty micrograms of protein were separated by 4-15% Mini-PROTEAN TGX Precast Gels (PAGE) (Bio-Rad, Hertfordshire, UK) and transferred to a nitrocellulose membrane ...
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bioRxiv - Microbiology 2019Quote: ... β-galactosidase activity units were normalized to total proteins per ml as determined using Bradford assay reagents (Bio-Rad). Assays were performed in biological triplicates.
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bioRxiv - Biochemistry 2019Quote: ... Proteins in whole-cell lysates were separated on 4-15 % Mini-Protean TGX Precast gels (456-1085, Bio-Rad) and transferred to 0.2 μm Nitrocellulose membranes (1704158 ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates containing 30 μg protein were subjected to SDS-PAGE using 10% Criterion TGX Stain-Free gels (BioRad, UK) on a mini-Protean Tetra system (Bio-Rad ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and 15μg protein in 5x Laemmli buffer with 5% b-mercaptoethanol was loaded on Mini-PROTEAN TGX Precast Gels (BioRad). Gel lanes were cut into three sections for peptide extraction ...
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bioRxiv - Molecular Biology 2020Quote: ... equal amounts of total protein were loaded onto 4–20% SDS-PAGE gels (Mini-PROTEAN TGX Gel, Bio-Rad). After SDS-PAGE ...
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bioRxiv - Neuroscience 2021Quote: ... Equivalent amounts of protein were subjected to electrophoresis on a 4-12% Mini-PROTEAN® TGXTM Gel (Bio-Rad) and transferred to a Hybond-ECL nitrocellulose membrane (Amersham - GE Healthcare Life Sciences) ...
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bioRxiv - Plant Biology 2020Quote: ... Protein concentrations were determined using the Bio-Rad Assay according to the manufacturer’s instructions (Bio-Rad Laboratories, Munich, Germany) based on the method of (48).
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bioRxiv - Biochemistry 2020Quote: ... Protein samples were then separated on 12 % polyacrylamide gels (Mini-Protean II electrophoresis cell, BioRad, Marnes-la-Coquette, France) and transferred to 0.2 μm nitrocellulose blotting membrane (GE Healthcare ...