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Citations for Bio-Rad :
8101 - 8150 of 9412 citations for Recombinant Human PDCD1LG2 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... The protein extracts were subjected to appropriate concentrations of SDS–PAGE for electrophoresis and transferred to PVDF membranes (Bio-Rad). Membranes were blocked with 5% bovine serum albumin (ThermoFisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... Total of 15-30µg protein were separated on a 4-12% Bis-Tris SDS PAGE and transferred using BioRad Turbo Blot (1704156, BioRad) onto PVDF membrane ...
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bioRxiv - Genetics 2022Quote: ... Proteins were separated by SDS-PAGE with 2-mercaptoethanol as a reducing agent using criterion sized 4-15% gradient tris-glycine for broad range of molecular weights greater than 30 kDa or 16.5% tris-tricine for high resolution of smaller proteins (Bio-Rad). Proteins were transferred to nitrocellulose membranes and probed with antibodies [commercially obtained except for ATF6 [63] ...
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bioRxiv - Cancer Biology 2022Quote: Protein separations were transferred onto a nitrocellulose membrane using the Trans-blot Turbo RTA transfer kit (Bio-rad, #170-4270) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: Proteins were resolved in 6-8-10 or 12% polyacrilammide gels and transferred to PVDF (Bio-Rad, Hercules, CA,USA) or nitrocellulose membranes (GE Heath Care ...
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bioRxiv - Biochemistry 2022Quote: Proteins were resolved by SDS/PAGE and transferred to nitrocellulose membranes using the Trans-Blot Turbo transfer system (Bio-Rad). Membranes were blocked with 5% BSA in Tris-buffered saline containing Tween 20 (TBS-T ...
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bioRxiv - Neuroscience 2022Quote: ... Aliquots of the lysates containing 10 mg of protein were denatured in Laemmli buffer and β-mercaptoethanol (Bio-Rad, USA) at 95°C for 5 min ...
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bioRxiv - Neuroscience 2022Quote: ... The protein concentration for each condition was measured directly from 1 µL aliquot of the sample using NanoDrop (record A280nm measurements and use the exact same protein sample buffer as blank) and the Bradford assay (Biorad). After incubation for different amounts of time (1 hour after dialysis or dilution to allow droplet maturation ...
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bioRxiv - Physiology 2022Quote: ... Lysates were boiled and 20-60μg of protein was loaded onto Mini-PROTEAN® TGX™ Precast Gels (Bio-Rad). SDS-PAGE gels ran for 50 minutes at 125 V ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The proteins were then transferred to a nitrocellulose membrane on a Trans-Blot® Turbo (Bio-Rad; Hercules, CA, USA). The membrane was blocked in a PBS solution containing 5% non-fat milk and 0.1% Triton X-100 for 1 h with orbital agitation ...
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bioRxiv - Microbiology 2022Quote: ... 10 μg of protein was separated by SDS-PAGE using 12% acrylamide Mini-protean TGX precast gels (Bio-Rad, USA). Gels were stained with Coomassie (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... Ten micrograms of protein was run per sample on 4–15% Mini-Protean TGX stain-free precast gels (Bio-Rad) at 150 V ...
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bioRxiv - Genetics 2022Quote: ... 60 μg of protein was separated on 10% SDS/PAGE (Mini-PROTEAN TGX Gels,12%,10-well, 4561043, BIO-RAD) and transferred onto nitrocellulose membrane (#1620115 ...
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bioRxiv - Developmental Biology 2022Quote: ... Protein samples were loaded on 10%-12% polyacrylamide gels and transferred onto 0.45 µm pore-size nitrocellulose membranes (Bio-Rad) using the Turboblot (BioRad) ...
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bioRxiv - Biophysics 2022Quote: Binding studies of various mRBD mutants with ACE2-hFc neutralizing antibody were carried out using the ProteOn XPR36 Protein Interaction Array V.3.1 from Bio-Rad as described previously (35) ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... and proteins were transferred to a PVDF membrane by using semi-dry Trans-Blot Turbo Transfer System (BioRad Laboratories, USA). Then the membranes were transferred into blocking solution (10% BSA or skimmed milk powder in PBS with 0.1% Tween20) ...
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bioRxiv - Biochemistry 2022Quote: ... Precast stain-free gradient gels for tryptophan fluorescence (4568084) as well as Precision Plus Protein Standards (1610393) were purchased from Biorad. Pyruvate kinase (P7768) ...
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bioRxiv - Biophysics 2022Quote: ... The protein sample was incubated at 40°C and 80 °C respectively for 1 h in a PCR cycler (BioRad) with a heated lid to prevent evaporation ...
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bioRxiv - Molecular Biology 2022Quote: ... The total protein were separated via 8% SDS-PAGE and transferred to polyvinylidene difluoride (PVDF, Bio-Rad, Cat no. #1620177) membrane using transfer buffer (25 mM Tris ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were eluted by heating the beads at 95°C for 5 min in Laemmli Sample Buffer (1610747, Bio-Rad) with 50 mM DTT followed by magnetic separation ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein samples were resolved by SDS-PAGE according to standard protocol and transferred onto 0.45μm nitrocellulose membranes (Bio-Rad 1620115) and blotted with the primary and secondary antibodies as described.
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bioRxiv - Cell Biology 2022Quote: ... 15-35 µg protein were loaded and separated by SDS-PAGE in 4–20% Mini-Protean TGX Precast gels (BioRad). Gels were transferred to PVDF membranes using the iBlot system (Thermo) ...
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bioRxiv - Cancer Biology 2022Quote: ... The protein pellet was resuspended in 50 μL 8 M urea in 50 mM ammonium bicarbonate and the protein concentration determined using a Bradford assay (BioRad). Proteins were reduced using 10 mM dithiothreitol for 30 min at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... 750 ng of wtXLF and each tandem dimer construct were loaded and run on a 4-15% Mini-PROTEAN TGX Precast Protein Gel (BioRad). tdXLF constructs where the KBM of subunit 1 was replaced with additional linker sequence (lane 4 tdXLFΔKBM/WT and lane 6 tdXLFΔKBM/ΔKBM ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were transferred onto a nitrocellulose membrane using Trans-Blot® Turbo™ RTA Midi Nitrocellulose Transfer Kit (Bio-Rad) in the Trans-Blot® Turbo™ Transfer System (Bio-Rad) ...
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bioRxiv - Neuroscience 2021Quote: ... Between 3 and 10 μg of total protein were loaded on to a 4-15% gradient TGX gel (Bio-Rad) and transferred on to a PVDF membrane (Bio-Rad) ...
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bioRxiv - Neuroscience 2021Quote: ... Equal amounts of whole protein from each sample was subjected to SDS-PAGE using the TGX Stain-Free FastCast Acrylamide kit (BioRad). Protein bands were transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Plant Biology 2021Quote: ... Protein extracted from tobacco or kiwifruit were separated on 12% SDS-PAGE gels and transferred to PVDF membranes (Bio-Rad) using a highly efficient wet protein transfer system (eBlot L1 ...
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bioRxiv - Neuroscience 2020Quote: ... 0.02% bromphenol blue) for 15min at 50°C and 5μg of protein were separated on a 4-20% SDS PAGE (Biorad, #4561096) and blotted onto nitrocellulose membranes by wet transfer ...
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bioRxiv - Microbiology 2020Quote: ... After electrophoresis the separated proteins were electroblotted onto a PVDF membrane using a Trans-Blot Turbo Transfer System (Bio-Rad) with a standard protocol for seven minutes ...
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bioRxiv - Developmental Biology 2021Quote: Protein samples were loaded on SDS-PAGE gel and then were transferred to Nitrocellulose membrane (Bio-Rad, Cat. Nr. 1620112). After blocking for 1 h at RT ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were separated on a 10% SDS-polyacrylamide gel and then transferred to a nitrocellulose membrane (BioRad, Boston, MA, USA). The membrane was incubated with the appropriate primary antibody and then incubated with a species-appropriate secondary antibody ...
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bioRxiv - Molecular Biology 2020Quote: ... and adjusted before isolation of protein aggregates as well as verified by running reduced samples on Criterion XT Precast Gel 4-12% Bis-Tris (BioRad) and staining with Coomassie Brilliant Blue R-250 staining solution (BioRad) ...
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bioRxiv - Biophysics 2020Quote: ACE2-hFc and CR3022 neutralizing antibody binding studies with the various RBD derivatives purified from different expression platforms were carried out using the ProteOn XPR36 Protein Interaction Array V.3.1 from Bio-Rad. Activation of the GLM sensor chip was performed by reaction with EDC and sulfo-NHS (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... The protein from the 5% of beads was eluted by boiling the beads in Laemmli sample buffer supplemented with BME (BioRad) and 2 mM biotin at 90°C for 10 min ...
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bioRxiv - Plant Biology 2021Quote: ... proteins from SDS-PAGE gels were transferred onto nitrocellulose membranes using the Transblot Turbo RTA Midi Transfer kit (BioRad, USA). Mouse anti-α-tubulin antibody (Sigma-Aldrich ...
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Peripheral neuropathy linked mRNA export factor GANP reshapes gene regulation in human motor neuronsbioRxiv - Neuroscience 2021Quote: ... 5 µg of protein was loaded per well on a 10% TGX 10-well 30µl/well gel (456-1033, Bio-Rad) for 1 hr ...
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bioRxiv - Biochemistry 2021Quote: ... the gels were either stained with One-Protein Blue gel stain (#21003-1L, Bioticum) or transferred onto PVDF membranes for immunodetection using TurboBlot system (Biorad).
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bioRxiv - Plant Biology 2021Quote: ... 20 μg total protein was separated on 10% SDS-PAGE gels and blotted onto Immun-Blot PVDF membrane (Bio-Rad). LSD1-GFP ...
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bioRxiv - Physiology 2021Quote: ... Proteins (50 μg) were loaded onto 12–15% SDS-PAGE gels using a Mini-Protean apparatus (Bio-Rad, CA, USA) and transferred onto nitrocellulose membranes (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: ... Lämmli buffer was added and identical protein amounts of the different samples were loaded on TGX Stain-free Precast gels purchased from Biorad. After the run the gels were activated by a 45 sec UV exposure and electroblotted onto nitrocellulose membranes ...
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bioRxiv - Physiology 2021Quote: ... Approximately 10 µg of protein was loaded into a 4% - 20% Criterion pre-cast gel (Bio-Rad, Hercules, CA, USA) and resolved at 120 V for 120 min ...
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bioRxiv - Neuroscience 2021Quote: ... We ran 40 µg of protein on 10% gels using the Mini-PROTEAN Tetra Cell western blotting system (Bio-Rad). Anti-NaV1.1 (Ab5204a ...
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bioRxiv - Microbiology 2021Quote: ... Twice the amount of lysate was loaded onto gels for use in Western blotting and proteins were transferred to pre-equilibrated polyvinylidene difluoride (PVDF) membranes (BioRad) at 20 volts for 60 minutes using the BOLT transfer system (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2021Quote: ... proteins were transferred to 0.45 μM nitrocellulose membranes using Trans-Blot® Turbo™ Transfer System Cell system (Bio-Rad). The membrane was then blocked in 5% nonfat dry milk TBS 0.05% Tween 20 and incubated with primary antibody overnight at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... An equal amount of total protein (30μg) were resolved on precast 4-12% SDS-PAGE gels (Biorad, Hercules, CA, USA), transferred onto nitrocellulose membranes ...
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bioRxiv - Neuroscience 2022Quote: ... the membrane was then treated for 45 min at room temperature with the appropriated HRP-conjugated secondary antibody: Proteins were visualized using an ECL+ detection kit (Amersham) and imaged using a Chemidoc Touch Imaging system (Biorad). Densitometry was analyzed using the ImageLab software.
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Sparcl1/Hevin drives inflammatory and neuropathic pain through astrocyte and NMDA receptor signalingbioRxiv - Neuroscience 2022Quote: ... and 30 μg of proteins were loaded for each lane and separated on SDS-PAGE gel (4–15%; Bio-Rad). After the transfer ...
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bioRxiv - Physiology 2022Quote: ... Total protein (30 μg) per lane was separated by SDS-PAGE using 4–15% Tris-glycine gels (Bio-Rad Laboratories) and transferred to PVDF membrane (Millipore Sigma) ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were normalized for protein concentration and subjected to SDS-PAGE separation on stain-free tris-glycine gels (Bio-Rad), cross-linked and imaged with the Chemidoc system (Bio-Rad) ...