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Citations for Bio-Rad :
8051 - 8100 of 9925 citations for Recombinant Rabbit IL17F Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2020Quote: ... and isolated protein complexes were eluted from the beads in 1X SDS sample buffer (Bio-Rad Laboratories, Hercules, CA) at 60°C for 10 min ...
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Functional characterization of a DNA-dependent AAA ATPase in SimranZ1, a F cluster mycobacteriophagebioRxiv - Biochemistry 2021Quote: ... The protein was then transferred to a PVDF membrane by using Mini-PROTEAN Tetra Cell (Bio-Rad, United States). The membrane was blocked overnight by using 5% BSA at 4 °C followed by incubation with mouse anti-His6 antiserum (Santacruz Biotech ...
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bioRxiv - Biochemistry 2021Quote: ... and the proteins were purified using a Profinia immobilized metal affinity chromatography (IMAC) column with Ni2+ beads (Bio-Rad) and a Profinia protein purification system (Bio-Rad ...
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bioRxiv - Biochemistry 2020Quote: ... His-tagged protein was detected using a mouse anti-6×His IgG conjugated to horseradish peroxidase (MCA1396P; Bio-Rad), and the blot was developed using Pierce™ ECL Western Blotting Substrate (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated using 12% polyacrylamide gels and transferred onto a PVDF membrane using Trans-Blot Turbo (Bio-Rad). The following primary antibodies were used ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were then electroblotted onto a PVDF membrane using a semidry blotter (trans-blot turbo transfer system; Bio-Rad). Membranes were blocked overnight at 4°C in TBS-T (50 mM Tris-HCl ...
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bioRxiv - Microbiology 2020Quote: ... 40 µL of supernatant containing 5 µg of proteins were mixed to 36 μL Laemmli Buffer 1x (Bio-Rad) and 4 µL β-mercaptoethanol BioUltra (Sigma ...
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bioRxiv - Plant Biology 2021Quote: ... and 30 μg of total protein was separated by 12 % SDS-PAGE and blotted onto PVDF membrane (Bio-Rad). The membranes were blocked in 5 % (w/v ...
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bioRxiv - Systems Biology 2020Quote: ... As a molecular weight marker we utilized the Precision Plus Protein™ Prestained Standard in Dual Color (Biorad, 1610374).
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bioRxiv - Neuroscience 2020Quote: ... Immunoprecipitated samples were separated by SDS-PAGE using Any kD Mini-PROTEAN TGX Precast Protein Gels (Bio-Rad, 4569034), and then blotted to a PVDF membrane (0.2 μm ...
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bioRxiv - Neuroscience 2020Quote: ... The protein concentration of each homogenate was measured using a Bradford assay according to the manufacturer’s instructions (Bio-Rad).
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bioRxiv - Microbiology 2021Quote: ... Proteins were then transferred at room temperature (RT) onto a nitrocellulose membrane using a semi-dry transfer cell (Biorad) (20 V ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein bands were then transferred from the gels onto 0.2 μm polyvinylidene fluoride (PVDF) membranes (Bio-Rad, CA, USA) using semi-dry electroblotting system (Trans-Blot Turbo Transfer System ...
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bioRxiv - Microbiology 2020Quote: ... 7.5 mg of proteins were diluted in ChIP buffer supplemented with 0.01% SDS and precleared 1 h at 4 °C with 50 μl of protein A agarose beads (BioRad) and 100 μg BSA ...
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bioRxiv - Microbiology 2020Quote: ... Equal quantities of protein (generally 20-50 μg) were resolved on 4-20% gradient SDS-Polyacrylamide gels (Bio-Rad) and then transferred onto Polyvinylidene difluoride membranes (Millipore Sigma) ...
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bioRxiv - Microbiology 2020Quote: ... The concentration of the epithelial cell proteins in the resulting supernatant was determined using the Bradford method (Bio-Rad).
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bioRxiv - Cancer Biology 2020Quote: ... Twenty μg of each protein sample was separated using SDS-gel electrophoresis and transferred onto a 0.45-μm PVDF membrane (BioRad). Membranes were incubated with blocking buffer (5% milk powder dissolved in 1x TBST ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were electrophoresed at 150V for one hour through 7.5% or 4-20% mini-PROTEAN TGX gels (Bio-Rad) and blotted at 300 mA for one hour onto PVDF membranes ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were resolved in SDS-PAGE gel (4-20% gradient polyacrylamide gel electrophoresis, Mini-PROTEAN® Precast Gels, Biorad). Blots were blocked for 1 hour with 5% non-fat dry milk in PBS/0.1% tween-20 (Sigma Aldrich) ...
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bioRxiv - Cancer Biology 2021Quote: Protein lysates from SARS-CoV-2 infected Vero E6 cells were harvested in 2x Laemmli sample buffer (Bio-Rad) containing 2-Mercaptoethanol (Fisher Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell lysates and lipid raft protein isolates were prepared by sonication in 4x Laemmli sample buffer (Bio-Rad, 1610747) and then loaded into 10% SDS-polyacrylamide gels for electrophoresis ...
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bioRxiv - Cell Biology 2021Quote: ... the lysate that contains 10 μg of protein per sample was mixed with 2x Laemmli Sample Buffer (Bio-Rad) supplemented with 2 M 2-Mercaptoethanol (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: ... Total protein levels were quantified to normalize between each well using Bradford reagent (Bio-Rad, Marnes-la-Coquette, France).
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bioRxiv - Systems Biology 2022Quote: ... The supernantant was then added to a new Eppendorf and protein concentration was assessed using the Bradford assay (Biorad).
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bioRxiv - Cancer Biology 2022Quote: ... samples were heated for 5’ at 95°C in Lämmli buffer and protein lysates were resolved on polyacrylamide Mini-PROTEAN TGX gels (BioRad) and transferred onto nitrocellulose membrane by wet-transfer ...
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bioRxiv - Cancer Biology 2022Quote: ... Purified proteins were visualized on a 4-15% gradient SDS-PAGE TGX Stain-Free gel (Bio-Rad 456-8086) and then stained with Coomassie blue (Bio-Rad 1610786) ...
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bioRxiv - Biophysics 2022Quote: ... The protein-dye conjugate was purified by 3 rounds of size exclusion chromatography (Bio-Spin 6 column, BioRad, CA) and then aliquoted in 5 μL portions and stored at -80C until required.
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bioRxiv - Molecular Biology 2022Quote: ... under reducing conditions and proteins transferred to nitrocellulose membranes using a semidry Trans-Blot Turbo Transfer System (Bio-Rad). Antibody reactions were carried out in 1% skimmed milk in PBS with 0.1% Tween-20 and washed in PBS with 0.1% Tween-20 ...
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bioRxiv - Neuroscience 2022Quote: ... Lysates were then centrifuged at 12,000 rpm at 4°C for 20min and total protein extracts were boiled in Laemmli sample buffer (BioRad), separated by SDS-PAGE under reducing conditions and transferred to PVDF membranes (Millipore) ...
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bioRxiv - Plant Biology 2022Quote: ... proteins were electrophoretically transferred onto a polyvinylidene fluoride (PVDF) membrane using Semidry Trans-Blot apparatus (Bio-Rad, Hercules, CA) at 20 V for 20 min ...
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bioRxiv - Microbiology 2022Quote: ... in equal volume and then subjected to electrophoresis using the 7.5% Mini-Protean TGX Precast Protein Gels (Bio-RAD) in a non-denaturing buffer ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were separated using a gradient SDS PAGE gel (8-12%) and a Mini-Protean 3 Cell (Bio-Rad) at 85 V ...
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bioRxiv - Microbiology 2022Quote: ... total protein extracts were subjected to SDS page electrophoresis using 4-20% polyacrylamide gradient gels (Bio-Rad, Watford, UK) and transferred to nitrocellulose membranes using a Trans-blot Turbo transfer system (Bio-Rad) ...
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bioRxiv - Biophysics 2022Quote: ... 10 µL of 12x and 120x diluted protein prep was then loaded into a 15% SDS-PAGE gel (BioRad) with BioRad Precision Plus unstained standard for comparison ...
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bioRxiv - Biochemistry 2022Quote: ... 40 μg of total protein was separated by SDS-PAGE and transferred to a PVDF membrane (Bio-Rad 1620177). Membranes were blocked for 1 h in 5% milk in 1X PBST (or 5% BSA in 1X PBST for western blots of immunoprecipitations ...
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bioRxiv - Microbiology 2022Quote: ... and 4 µg of protein was separated by SDS-PAGE using pre-cast TGX 4-15% gradient gels (BioRad). Protein was transferred to a 0.45 µm nitrocellulose membrane ...
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bioRxiv - Biochemistry 2022Quote: ... 1 μL protein was injected onto either a self-packed buffer exchange column (P6 polyacrylamide gel, Bio-Rad Laboratories) or a prototype desalting column from Thermo Fisher Scientific and online buffer exchanged to 200 mM ammonium acetate ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were transferred from the remainder of the gel to nitrocellulose membrane using the Trans-Blot Turbo (Bio-Rad). Membranes were incubated in a 5% milk in 1X Tris-buffered saline ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins where eluted from the beads by directly adding 20 µL of Laemli Sample Buffer buffer 2X (Biorad, #1610747). Samples where then processed for SDS-PAGE and Western blotting.
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bioRxiv - Biochemistry 2020Quote: ... Protein bands were quantified on a BioRad Chemidoc Touch Imaging System using Image Lab software version 6.0.1 (Bio-Rad).
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bioRxiv - Cell Biology 2019Quote: ... Data were normalized to GAPDH expression or total protein determined via UV treatment of TGX Stain-Free gels (Biorad) followed by detection on the transfer membrane.
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bioRxiv - Developmental Biology 2020Quote: ... Proteins were separated on a SDS-PAGE Mini-PROTEAN TGX 4-15% gradient gel (Bio-Rad, catalog #456-1086) along a 1:1 mix of Amersham ECL Rainbow Molecular Weight Markers (Amersham # 95040-114 ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were denatured and proteins separated in SDS-PAGE in Criterion™ TGX Stain-Free™ Gels (Bio-Rad), and transferred onto nitrocellulose membranes with the Trans-Blot® Turbo™ Blotting System (Bio-Rad).
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bioRxiv - Biochemistry 2021Quote: ... The protein was incubated at 4°C for 1 h with Bio-Beads™ SM-2 resin (Bio-Rad) equilibrated with 100 mM Tris-HCl ...
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bioRxiv - Neuroscience 2020Quote: ... Fifteen μg of protein per sample were separated in 4-20% SDS-polyacrylamide gel (BIO-RAD mini-protean-TGX) before electrophoretic transfer onto a nitrocellulose blotting membrane (Amersham ...
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bioRxiv - Microbiology 2021Quote: ... the remaining interphase was dried completely before resuspending in Tris-buffered saline (50mM Tris-Cl, 150mM NaCl, pH 7.6) for protein quantification using a Bradford Assay (BioRad). Aqueous phase was evaporated under nitrogen flow and used for LC-MS ...
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Transcriptome analyses reveal tau isoform-driven changes in transposable element and gene expressionbioRxiv - Neuroscience 2021Quote: ... 12.1 ug of protein from each sample was loaded onto a 10% Tris-Glycine gel (Biorad, cat no. 4561034) and then transferred onto a PVDF membrane and blocked for one hour with 5% BSA in 0.1% Tween/1xTBS ...
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bioRxiv - Neuroscience 2021Quote: ... Both the soluble (40 µg of proteins) and the insoluble (4 µl) fractions were spotted on nitrocellulose membrane (BioRad). The near infrared fluorescence signal was acquired by scanning the membrane at the LiCor Odissey using the 800 nm filter and images were analyzed using the Fiji software.
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bioRxiv - Microbiology 2020Quote: ... and the images were analyzed and the signals quantitated after total cell protein normalization using the Image Lab 6.0.1 software (Bio-Rad).
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bioRxiv - Microbiology 2020Quote: ... and 150 ml Protein A Sepharose (GE, 17-5138-01) in a Poly-Prep Chromatography Column (BioRad, 731-1550). Fabs were generated by Papain cleavage and purified by the use of the Pierce Fab Preparation Kit according to the manufacturers’ instructions (Thermo Scientific - #44985) ...