Labshake search
Citations for Bio-Rad :
751 - 800 of 2206 citations for Mouse Anti Human Ig kappa chain Alexa Fluor 647 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2020Quote: SARS-COV-2 RBD-SD1 gBlock (IDT) was amplified by polymerase chain reaction (PCR) with iProof High-Fidelity PCR system (Bio-Rad, Cat # 1725310) following the manufacturer’s recommendations ...
-
bioRxiv - Cell Biology 2021Quote: ... Reverse Transcription – Quantitative Polymerase Chain Reaction (RT-qPCR) reactions were performed using SsoAdvanced™ universal SYBR® green (Bio-Rad, Hercules, CA, USA) and run on the CFX Connect (Bio-Rad ...
-
bioRxiv - Molecular Biology 2020Quote: ... Quantitative real time polymerase chain reaction for all targets was performed on the CFX-96 Real Time System using iQ SYBR Green Supermix (Bio-Rad, Hercules, CA). Products were confirmed by gel electrophoresis ...
-
bioRxiv - Neuroscience 2021Quote: ... was used from each sample for One-step reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) using the iTaq™ Universal SYBR® Green One-Step kit (Bio-Rad) in the StepOnePlus™ Real Time PCR system (Applied Biosystems) ...
-
bioRxiv - Genetics 2022Quote: ... Quantitative RT-polymerase chain reaction (qRT-PCR) experiments were performed using the CFX96 Touch Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules) with PowerUP SYBR Green Master Mix (Applied Biosystems ...
-
bioRxiv - Genetics 2022Quote: ... Quantitative RT-polymerase chain reaction (qRT-PCR) experiments were performed using the CFX96 Touch Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules) with PowerUP SYBR Green Master Mix (Applied Biosystems ...
-
bioRxiv - Physiology 2022Quote: For the separation of myosin heavy chain isoforms a large format SDS-PAGE setup with integrated cooling core was used (Protean II xi, Bio-Rad Laboratories, Inc.). A 5% stacking gel ...
-
bioRxiv - Physiology 2022Quote: ... Primers flanking the target site were used to amplify the target region of the gene in a 10 µL polymerase chain reaction (PCR) containing: 5 µL 2 × SuperMix (Bio-Rad, Hercules, CA), 1 µL genomic DNA ...
-
bioRxiv - Molecular Biology 2023Quote: Relative genetic expression was determined using Real Time Quantitative Polymerase Chain Reaction (RT-qPCR) (CFX96™ Real-Time System, Bio-Rad, CA, USA) using commercially available TaqMan primers (Tables 1-3 ...
-
bioRxiv - Immunology 2023Quote: ... Viral RNA genome was detected and quantified by reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) on a BioRad real-time CFX96 detection system (BioRad, Hercules, United States). The target sequence for amplification was the viral RNA-dependent RNA polymerase [26].
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Quantitative real time polymerase chain reaction (qPCR) was performed using the iQ™ SYBR® Green Supermix (170888, Bio-Rad, Hercules, CA, USA) according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2024Quote: ... We optimized the recommended polymerase chain reactions (PCR) cycling conditions and performed a touchdown PCR in a T100 thermocycler (Bio-Rad, Hercules, CA). The total reaction volume was 50 µl which contained 2 µl of genomic DNA at 50 ± 25 ng/µl ...
-
bioRxiv - Genetics 2021Quote: ... or horseradish peroxidase-conjugated goat anti-mouse secondary antibody conjugate (1:2000 dilution, Bio-Rad laboratories, #1706516). Membranes were incubated in ClarityTM western ECL substrate (Bio-Rad laboratories ...
-
bioRxiv - Developmental Biology 2020Quote: ... blots were incubated with either horseradish peroxidase (HRP)–conjugated goat anti-mouse antibody (Bio-Rad 170-6516) or HRP–conjugated goat anti-rabbit antibody at a concentration of 1:5000 ...
-
bioRxiv - Microbiology 2021Quote: ... followed by secondary HRP- conjugated goat anti-mouse IgG(H+L) (1:10,000 dilution; Bio-Rad, USA), and then developed using the enhanced chemiluminescence procedure (Pierce ...
-
bioRxiv - Microbiology 2021Quote: ... and then incubated for at least 16 hours at 4°C with either mouse monoclonal anti-His (1:2,000; AD1.1.10, Biorad) or mouse anti-RNA pol (1:5,000 ...
-
bioRxiv - Cell Biology 2020Quote: ... Horseradish peroxidase (HRP)-conjugated goat anti-mouse (#176516; 1:10,000 for Western blot) was from Bio-Rad. Alexa Fluor-conjugated goat antibodies against mouse IgG (1:500 for IF ...
-
bioRxiv - Cell Biology 2020Quote: ... HRP secondary antibodies were used as follows: 1:500 to 1:1000 anti-mouse (BioRad, 170–6516) and 1:500 to 1:1000 anti-rabbit (BioRad ...
-
bioRxiv - Microbiology 2022Quote: ... Membranes were washed before incubation with 1:2,500 goat anti-mouse StarBright™ Blue 700 (Bio-Rad).
-
bioRxiv - Neuroscience 2021Quote: Membranes were subsequently probed with horseradish peroxidase (HRP)-conjugated goat anti-mouse or -rabbit IgG (Bio-Rad), diluted 1:5000 in 5% (w/v ...
-
bioRxiv - Immunology 2022Quote: ... The washed membranes were incubated with secondary antibody StarBright Blue 700 Goat anti-mouse IgG (Bio-rad). The proteins were visualized by fluorescence using ChemiDoc MP Imaging Systems (Bio-Rad).
-
bioRxiv - Bioengineering 2019Quote: ... Blots were incubated the following day with 0,33μg/ml Goat-anti-rabbit or -mouse HRP (Bio-Rad) in blocking buffer for 1h at room temperature ...
-
bioRxiv - Synthetic Biology 2019Quote: ... was used as the primary antibody and Immun-Star Goat Anti-Mouse (GAM)-HRP conjugate (Bio-Rad) was used as the secondary antibody ...
-
bioRxiv - Cell Biology 2019Quote: ... Secondary antibodies used for westerns were: goat anti-mouse IgG horseradish peroxidase (HRP) conjugate (Bio-rad, 1705047) at 1:2000 and goat anti-rabbit IgG HRP conjugate (Bio-rad ...
-
bioRxiv - Cell Biology 2021Quote: Mouse monoclonal anti-Viral V5-TAG antibody (1:5000; clone SV5-Pk1) was purchased from Bio-Rad/AbD Serotec ...
-
bioRxiv - Immunology 2021Quote: ... the membrane was incubated with secondary antibodies Goat Anti-Mouse IgG (H+L)-HRP Conjugate (Bio-Rad) diluted 1:5000 in TBST with 1% BSA for 1 h at RT ...
-
bioRxiv - Cell Biology 2022Quote: ... as a primary antibody and HRP conjugated goat anti-mouse IgG secondary antibody (BIO-RAD, Catalog # #1706516). Chemiluminescence was detected using film for figure S1 and an Azure c400 imaging system (Azure Biosystems ...
-
bioRxiv - Molecular Biology 2022Quote: ... Horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (H+L) secondary antibody was purchased from Bio-Rad (catalog number ...
-
bioRxiv - Immunology 2022Quote: ... The following antibodies were used: mouse anti-guinea pig CD8 (clone MCA752F, Bio-Rad Laboratories, Hercules, CA), mouse anti-guinea pig CD4 (clone MCA749PE ...
-
bioRxiv - Neuroscience 2023Quote: ... and mouse anti-MHC Class I RT1A (#MCA51G, 1:100, clone: OX-18, 1:100, Bio-Rad). Sequential imaging was performed on an Olympus FluoView confocal laser-scanning microscope (FV1200 ...
-
bioRxiv - Genetics 2023Quote: HRP secondary antibodies were used as follows: 1:500 to 1:1000 anti-mouse (BioRad, 170-6516), 1:500 to 1:1000 anti-rabbit (BioRad ...
-
bioRxiv - Microbiology 2024Quote: ... Secondary antibodies used for immunoblots were StarBright Blue 520 goat anti-mouse (IgG; 1:10,000; Bio-Rad) and Horseradish peroxidase conjugated-goat anti-mouse (IgG ...
-
bioRxiv - Molecular Biology 2024Quote: ... the membrane was incubated with an HRP conjugated goat anti-mouse IgG secondary antibody (Bio-Rad # 1706516) for 1 hour at room temperature ...
-
bioRxiv - Cancer Biology 2024Quote: ... Secondary antibodies used were goat anti-mouse horse radish peroxidase (HRP)-conjugated (1706516, Bio-Rad, RRID: AB_11125547) at 1:10,000 and goat anti-rabbit HRP-conjugated (1721019 ...
-
bioRxiv - Neuroscience 2021Quote: Autaptic murine striatal neurons (DIV 15-18) were incubated with 1 μg/ml human GABAAR mAb or control antibody (anti-CD52, Bio-Rad, #HCA175) at 37°C for 24 hours ...
-
bioRxiv - Developmental Biology 2024Quote: ... Horseradish peroxidase (HRP)-conjugated secondary antibodies used were goat anti-rabbit IgG or anti-mouse IgG (Bio-Rad, 170-6515 or 170-6516, 1:3,000). To prevent signal overlap from IgG heavy chain with eRF1 ...
-
bioRxiv - Cell Biology 2023Quote: ... were removed from the eluted samples post-purification and quantified using quantitative Real Time polymerase chain reaction using the Bio-Rad CFX 96™ Real-Time PCR Detection System (Bio-Rad, Hercules, CA) and Promega Plexor®HY Quantitation System (Promega Corporation ...
-
bioRxiv - Microbiology 2024Quote: ... The AMR genotypes of transconjugant colonies were determined by polymerase chain reaction (PCR) assays on a T100™ Thermal Cycler (Bio-Rad, Hercules, CA). Reaction volumes were 25 µL and consisted of 12.5 µL of Accustart II PCR Toughmix (Quantabio ...
-
bioRxiv - Immunology 2023Quote: IgE receptor cross-linking (IgECL) on HSMCs was accomplished through sensitization with 1 μg/mL chimeric human IgE anti-NP antibody (MCA333S, BIO-RAD, Hercules, CA) for 24 hours ...
-
bioRxiv - Cell Biology 2019Quote: ... blots were incubated with 1: 5000 dilution of HRP-conjugated anti-mouse/ rabbit IgG secondary antibodies (Bio-Rad) in 1% milk-TBST for another hour ...
-
bioRxiv - Neuroscience 2021Quote: ... membranes were rinsed and incubated with the fluorescent secondary antibodies StarBright Blue 700 anti-mouse (1:5.000, BioRad) and StarBright Blue 700 anti-rabbit (1:5.000 ...
-
bioRxiv - Genomics 2020Quote: ... Secondary antibody used were an HRP-conjugated goat anti-mouse or rabbit at 1:25,000 (BioRad, Hercules, CA).
-
bioRxiv - Genetics 2019Quote: ... followed by an anti-mouse (Abliance; 1/1,000) secondary antibody and then the Clarity Western ECL reagent (BioRad). Densitometric analysis was performed on non-saturated signals using the Image Lab™ software (BioRad).
-
bioRxiv - Molecular Biology 2020Quote: ... The membranes were washed extensively with PBS-T before incubated with goat anti-mouse-HRP (Bio-Rad Laboratories) or rabbit anti-sheep-HRP (Bio-Rad Laboratories ...
-
bioRxiv - Cell Biology 2021Quote: ... Fixed cells were labeled with a mouse monoclonal anti-Chlamydia LPS antibody (BioRad CF 6J12, Hercules, CA, USA), rinsed with 1x PBS ...
-
bioRxiv - Microbiology 2021Quote: ... blots were re-probed with rabbit anti-mouse IgG peroxidase-conjugated antibodies (Bio-Rad / AbD Serotec; Hercules, CA) diluted 1:40,000 in PBS-T.3 ...
-
bioRxiv - Microbiology 2021Quote: ... Fixed cells were labeled with a mouse monoclonal anti-Chlamydia LPS antibody (BioRad CF 6J12, Hercules, CA, USA), rinsed with 1x PBS ...
-
bioRxiv - Microbiology 2020Quote: ... the membranes were incubated with horseradish peroxidase (HRP) conjugated goat anti-rabbit or mouse secondary antibody (Bio-Rad) for 1 h at room temperature ...
-
bioRxiv - Bioengineering 2019Quote: ... Blots were subsequently incubated with 0.33 µg ml-1 goat-anti-rabbit or mouse horseradish peroxidase (Bio-Rad) in blocking buffer for 1 h at room temperature ...
-
bioRxiv - Bioengineering 2021Quote: ... Blots were subsequently incubated with 1:2000 dilution of goat-anti-rabbit or mouse horseradish peroxidase (Bio-Rad) in blocking buffer for 1 h at RT ...