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Citations for Bio-Rad :
751 - 800 of 1190 citations for Monocyclohexyl Phthalate Unlabeled 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... an equal number of cells were lysed by resuspension in an equal volume (100 μL per 1x106 cells) of hot Laemmli buffer (Bio-Rad). If cells could not be counted upon collection ...
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bioRxiv - Biochemistry 2023Quote: ... and heated to 100 °C for 5 min and resolved on 4-20% (w/v) gradient SDS-PAGE gels (Bio-Rad) with Tris-glycine buffer ...
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bioRxiv - Neuroscience 2023Quote: ... The cDNA was amplified using KAPA HiFi Hotstart ReadyMix PCR kit (Nippon Genetics) with T-100 Thermal Cycler (Biorad, Hercules, CA). The temperature profile of the reaction was 95 °C for 2 min ...
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bioRxiv - Biochemistry 2023Quote: ... in a 100 μL reaction for 2 hours at 37°C and purified using Micro-Bio P-30 spin columns (Bio-Rad). The radiolabeled probe was heated to 90°C for 2 minutes and resuspended in 10 mL of ULTRAhyb Oligo hybridization buffer (Thermo Fisher) ...
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bioRxiv - Microbiology 2023Quote: ... Lactose and salts were removed by gel filtration chromatography over a BioRad P2 column (100 cm x 316 mm, Bio-Rad) using a semi-automated fast protein liquid chromatography (FPLC ...
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bioRxiv - Cell Biology 2022Quote: ... 5% sorbitol synchronized ring stage Pf NF54 parasites at 5-8% parasitemia were electroporated with 100 µg of pFC-LINUP plasmid at 0.31 kV and 950 µF using a BioRad Gene Pulser (BioRad, Hercules, CA). Positive selection with 8 nM WR99210 (WR ...
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bioRxiv - Immunology 2023Quote: ... and 100 μL of the secondary antibody solution - Goat Anti-Mouse IgG (H+L)-HRP Conjugate (Bio-Rad, Hercules, CA, USA) diluted in a 1:2,000 ratio in the antibody diluent - was then added and the plates were incubated for 1 hour in the dark at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was then diluted 1:100 and 1.5 µL was use per 15 µL reaction with iTaq Universal SYBR Green Supermix (Bio-Rad # 1725122) and 250 nM primers (final ...
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bioRxiv - Physiology 2023Quote: ... and the cells were resuspended in permeabilization buffer consisting of intracellular-like medium (120mM KCl; 10mM NaCl; 1mM KH2PO4; 20mM HEPES; pH 7.2) that had been cleared with Chelex 100 (Bio-Rad # 1422822) to remove trace Ca2+ ...
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bioRxiv - Microbiology 2023Quote: ... flash frozen in liquid nitrogen and treated with a lysis buffer (150mM NaCl, 50mM Tris pH7.5, 1% Triton X-100 (Bio-Rad #1610407), 1% SDS ...
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bioRxiv - Microbiology 2023Quote: ... The reaction mixtures contained 100 ng RNA template per sample diluted 2-fold with qPCR solution (iTaq Universal SYBR Green One-Step Kit, Bio-Rad) containing the primers D2S and D2C ...
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bioRxiv - Physiology 2023Quote: ... and the pellet resuspended in 37.5µL of Na+-free intracellular-like medium (120mM KCl; 1mM KH2PO4; 20mM HEPES; pH 7.2) that had been cleared with Chelex 100 (Bio-Rad # 1422822) to remove trace Ca2+ ...
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bioRxiv - Physiology 2023Quote: ... and the pelleted cells resuspended in permeabilization buffer consisting of intracellular-like medium (120mM KCl; 10mM NaCl; 1mM KH2PO4; 20mM HEPES; pH 7.2) that had been cleared with Chelex 100 (Bio-Rad # 1422822) to remove trace Ca2+ ...
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bioRxiv - Microbiology 2023Quote: ... brucei PFs were prepared in 1% Triton X-100 in PBS with 1X protease inhibitor cocktail (Bio-Vision) and mixed in 4X Laemmli buffer (Bio-Rad) with 710 mM β-mercaptoethanol and heated for 5 min at 95°C ...
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bioRxiv - Neuroscience 2023Quote: ... The pellet was resuspended in 8M urea buffer (8 M urea, 100 mm NaHPO4, pH 8.5) and boiled in Laemmli sample buffer (BioRad, 161-0737) at 98°C for 5 min ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were boiled at 100°C for 5 min after addition of 50 µl of 4x Laemmli sample buffer (BioRad, 1610747) containing 2-Mercaptoethanol (BME) ...
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bioRxiv - Cell Biology 2024Quote: Proteins were wet-transferred to polyvinylidene difluoride (PVDF) membranes at 100 V for 1 h using a Mini Trans-Blot Cell (Bio-Rad). Membranes were blocked with either 5% milk (CD63 detection ...
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bioRxiv - Microbiology 2024Quote: ... 80µl of ice-cold bacterial cells were mixed with 1µl plasmid (pBAD28 == pEMPTY or pHND10 == pAPY, 100-200ng, key resources table) in chilled 0.1cm cuvettes (Bio-Rad, cat# 1652089) and immediately electroporated (Voltage = 1.8kV ...
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bioRxiv - Plant Biology 2023Quote: ... Protein extractions containing 5 µg of Chl with 1 x SDS loading buffer were boiled at 100℃ for 5 min and loaded onto a 4 - 20% polyacrylamide gel (Mini Protean TGX, Biorad Laboratories). Proteins were transferred to a PVDF-FL membrane on a Biorad semidry blotting system ...
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bioRxiv - Systems Biology 2020Quote: ... equipped with a Hitrap™ column of 5 mL (Bio-Rad, Hercules, CA, USA) was used to purify produced proteins ...
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bioRxiv - Immunology 2021Quote: ... Protein 1ug/ml were immobilized onto NC membranes (0.22-m pore size; Bio-Rad). The membrane kept for drying for 5-10 min ...
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bioRxiv - Biochemistry 2019Quote: ... the beads were transferred to a gravity-flow Econo column (49 ml volume; BioRad), washed with five column volumes (CV ...
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bioRxiv - Microbiology 2019Quote: ... washed with column buffer and then transferred to an empty column (BioRad 15 ml). Unbound protein was washed using until background OD280 reached ~0.06 ...
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bioRxiv - Biochemistry 2020Quote: ... and loaded onto with a volume of 10 mL column (Bio-Rad, Lab., Inc.) and buffer exchange done manually ...
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bioRxiv - Biochemistry 2020Quote: ... RNAP was then loaded on a 50 ml Bio-Rex 70 column (Bio-Rad) equilibrated with Bio-Rex buffer (10 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2021Quote: ... protein-bound beads were transferred to a 10-mL chromatography column (Bio-Rad 7311550) and washed with the following buffers ...
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bioRxiv - Genomics 2022Quote: ... and suspended to 106 cells/mL in PBS + 0.05% BSA (Bio-Rad cell counter) (Figure 1).
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bioRxiv - Microbiology 2022Quote: ... Recombinant His-tagged proteins were purified using a 5 mL IMAC column (Bio-Rad Laboratories ...
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bioRxiv - Biochemistry 2021Quote: ... pH 7.5 and loaded onto a 2 ml CHT2-I hydroxyapatite column (Bio-Rad) equilibrated in 20 mM HEPES ...
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bioRxiv - Molecular Biology 2020Quote: ... 15525-017)] and 900 mL ddH2O] and transferred to Polyvinylidene fluoride membranes (Bio-Rad, 162-0177) at 85 V for 1 hour in 1 X SDS-PAGE Transfer Buffer [100 mL of 10 × 1L Stock [30.275 g Tris Base (Fisher Scientific ...
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bioRxiv - Biochemistry 2019Quote: ... the matrix was transferred to a gravity-flow Econo column (49 ml volume; BioRad) and washed with buffer D [50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... The beads were transferred to 10 ml columns (Poly-Prep Chromatography Column, Bio-Rad) and eluted with LFB1/50 supplemented with 250 mM imidazole.
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bioRxiv - Plant Biology 2021Quote: ... and 1 ml Luminol/enhancer solution (Clarity™ Western ECL, BIORAD, Les-Ulis, France) and observed using ChemiDoc (ChemiDoc™ Imaging Systems ...
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bioRxiv - Cancer Biology 2023Quote: ... fresh 1ng/ml recombinant human fibroblast growth factor (FGF) basic (BioRad, Veenendaal, The Netherlands) and 0.1mM L-ascorbic acid (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... fresh 1ng/ml recombinant human fibroblast growth factor (FGF) basic (BioRad, Veenendaal, The Netherlands) and 0.1mM L-ascorbic acid (Sigma Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... blocked in 10% normal donkey serum, and then stained in antibodies overnight (rabbit anti-Iba1, 1:500, Wako and CD68 (Biorad 1:100)) ...
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bioRxiv - Biochemistry 2021Quote: ... The treated lysates were mixed with the 2 × SDS-PAGE sample buffer with or without 100 mM β-mercaptoethanol and run on 12% Mini-PROTEAN TGX Precast Gel (Bio-Rad) without a boiling step ...
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bioRxiv - Biochemistry 2021Quote: ... the reactant was mixed with the 2 × SDS-PAGE sample buffer containing 100 mM β-mercaptoethanol and run on 12% Mini-PROTEAN TGX Precast Gel (Bio-Rad) without a boiling step ...
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bioRxiv - Bioengineering 2021Quote: ... The resulting gene fragment was ligated with the plasmid backbone using a ligase-free ligation method developed in the lab that includes incubation with 10 mM MgCl2 in a thermocycling step in a PCR thermocycler (Biorad, T-100). The cycling programme was as follows ...
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bioRxiv - Cell Biology 2021Quote: ... except that blocking was done with phosphate-buffered saline plus 0.1% Triton X-100 (PBS-T) containing 5% nonfat dry milk (Biorad Blotting grade Blocker) and 10 mM NaN3 ...
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bioRxiv - Physiology 2020Quote: ... Blood amino acids were extracted from 500 uL of supernatant by cation exchange chromatography (Dowex AG 50W-8X, 100–200 mesh H+ form; Bio-Rad Laboratories). Phenylalanine enrichments were determined by gas chromatography–mass spectrometry (GC-MS ...
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bioRxiv - Microbiology 2021Quote: ... 100 ng plasmid DNA and 100 μL of electrocompetent mycobacteria were mixed and transferred to a 2 mm electroporation cuvette (Bio-Rad #1652082). Where necessary ...
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bioRxiv - Plant Biology 2021Quote: ... The resulting plasmids were coated on tungsten beads and used for biolistic transformation of the cells with the PDS-100/He system (Bio-Rad, 1672257). DNA from zeocin (100 µg/mL final ...
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bioRxiv - Neuroscience 2020Quote: ... and 25-100 μg of total protein was loaded onto 8 to 15 % SDS-PAGE mini gels (Bio-Rad Laboratories, Hercules, CA) prior transfer onto PVDF membranes ...
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bioRxiv - Developmental Biology 2021Quote: ... using the whole SingleShot™ Cell Lysis Kit (SingleShot™ Cell Lysis Kit, 100 x 50 µl reactions Cat# 1725080, Bio-Rad) as follows ...
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bioRxiv - Neuroscience 2019Quote: ... and samples were then transferred at 100 V at 4°C onto an activated 2 um polyvinylidene difluoride (PVDF) membrane (Bio-Rad 1620177), blocked with 3% BSA in 0.2% Tween-20 (Sigma P9614 ...
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bioRxiv - Pathology 2019Quote: ... Vertical electrophoretic separation utilized 12.5% polyacrylamide gels and the following current parameters: 600 V/30 mA/100 W in an electrophoretic chamber (PROTEAN® II xi, Bio-Rad). The obtained gels were subjected to a standard colouring procedure with silver in the presence of formaldehyde as a regulator ...
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bioRxiv - Cancer Biology 2019Quote: ... Isolated RNA (100 ng) was subjected to qRT-PCR analysis using iTaq™ Universal SYBR® Green One-Step Kit (Bio-Rad) performed on the 7500 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Plant Biology 2020Quote: ... and stopped at each of the above-mentioned time points by loading an 150 µl aliquot of the reaction mixture to an anion exchange resin column (acetate form, 100-200 mesh, Dowex AG1-X8 Resin, Bio-Rad, UAS). The resin column was previously equilibrated five times using 150 mM sodium acetate (pH=7.5) ...
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bioRxiv - Microbiology 2020Quote: ... containing 2 g l-1 glucose and 1.32 mM K2HPO4 by treating prepared media with a universal chelator (Chelex® 100 Chelating Resin, Bio-Rad 1421253), filtering sterilizing away the Chelex resin ...