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Citations for Bio-Rad :
751 - 800 of 1031 citations for Hepatitis C Virus Core Antigen HCcAg since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Proteins were heated in reducing Laemmli sample buffer at 95°C and resolved in SDS-PAGE protein gel and transferred to PVDF membrane (Bio-Rad). Membranes were blocked using 5% BSA (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... After centrifugation at 14000 rpm for 20 min at 4°C the supernatant was collected and protein concentration determined by the Bradford protein assay (Bio-Rad). Fifty micrograms of total extract were then loaded in SDS-polyacrylamide gels and transferred onto nitrocellulose membranes for western blot analysis ...
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bioRxiv - Cell Biology 2022Quote: ... separated into an 8%-12% SDS-PAGE gels and transferred to a nitrocellulose Hybond-C membranes using a Trans-Blot Turbo Transfer System (Bio-Rad). Membranes were blocked with 5% milk in PBS-0.1% Tween-20 (Sigma-Aldrich ...
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bioRxiv - Genomics 2022Quote: ... and incubated at 65°C for 3 mins before running 1% agarose-Etbr gel electrophoresis to detect the cleavage efficiency by ChemiDoc system (Bio-Rad).
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bioRxiv - Molecular Biology 2022Quote: ... The libraries were eluted from the gel by freezing the gel pieces at −20°C for 30 min in Freeze ‘N Squeeze DNA gel extraction spin columns (Bio-Rad) and recovering the solution by immediate centrifugation of the columns at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Samples were boiled for 5 min at 95°C before separation on a gradient 4 to 20% sodium dodecyl sulfate (SDS)-polyacrylamide gel (Bio-Rad). Samples were then transferred to nitrocellulose membrane using standard methods for semidry transfer (Bio-Rad) ...
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bioRxiv - Immunology 2022Quote: ... denatured for 10 min at 95 °C and loaded on a 10% SDS-polyacrylamide gel (SDS-PAGE) and separated for 45 min at 200 V (Bio-Rad). Samples were transferred to a nitrocellulose membrane (TransBlot Turbo ...
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bioRxiv - Microbiology 2022Quote: ... samples were incubated at 95°C for 5 or 10 min and then resolved on TGX Stain-free gel (Bio-Rad). The proteins were transferred onto 0.2 μm nitrocellulose membranes using Trans-Blot Turbo Transfer (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... samples were incubated at 95°C for 5 or 10 min and then resolved on TGX Stain-free gel (Bio-Rad). The proteins were transferred onto 0.2 µm nitrocellulose membranes using Trans-Blot Turbo Transfer (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were heated at 95 °C for 5 min and loaded on 12% Mini-PROTEAN TGX precast protein gel (#4561043, Bio-Rad). The gel was run using 1x Tris/Glycine running buffer at 100 V for 90 min ...
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bioRxiv - Microbiology 2023Quote: ... and treated with or without heat denaturation (96°C, 8 min) and ran on a TGX stain-free precast gel (Bio-Rad) for 38 min at 200 V ...
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bioRxiv - Microbiology 2022Quote: ... Reactions were 10 μL and conducted in 384-well plates using an annealing temperature of 60°C on a CFX384 Touch Real-Time PCR Detection System (Bio-Rad).
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bioRxiv - Physiology 2022Quote: ... Lysates were diluted with 4X Laemmli buffer containing beta-mercaptoethanol and boiled for 10 minutes at 95°C and subjected to SDS-polyacrylamide gel electrophoresis 4-20% (Criterion TGX Stain-Free Protein Gel, Bio-Rad) gels ...
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bioRxiv - Microbiology 2022Quote: ... Samples were heated 10 min at 95°C before loading 10 µl aliquots onto precast mini- PROTEAN TGX gels (Bio-Rad). The polyacrylamide concentration was 7.5% for GFP-DamX constructs ...
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bioRxiv - Microbiology 2022Quote: ... Samples were heated for 10 min at 95°C before loading 10 µl onto a 10% polyacrylamide precast mini-PROTEAN TGX gel (Bio-Rad). After electrophoresis the gel was washed twice with distilled water ...
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bioRxiv - Molecular Biology 2022Quote: ... The plate was then heat sealed with pierceable foil using a PX1 PCR plate sealer 5s at 180°C (Bio-Rad), and PCR amplification was carried out in a T100 thermal cycler (Bio-Rad) ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were heated at 95°C for 10 min and loaded into a 4-15% gradient Tris-glycine gel (Biorad 4561084) immersed in Tris-glycine-SDS running buffer (Biorad 1610772) ...
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bioRxiv - Physiology 2022Quote: ... followed by a final melting curve analysis from 70°C to 95°C with 0.2°C increment for each step using the CFX96 Real-Time PCR System and the Precision Melt Analysis software (Bio-Rad). For HMA analysis ...
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bioRxiv - Plant Biology 2022Quote: ... The dicing reactions were performed at 37 °C for 30 min and stopped by adding 1 volume TBE-Urea sample buffer (Bio-Rad), heating at 95 °C for 10 min and quickly chilling on ice for 2 min ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were grown at 30°C for 3-6 days and images captured using a Chemidoc XRS+ imager (BioRad, Hercules, CA). All images were evenly adjusted in Photoshop (Adobe Systems Incorporated ...
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β-catenin programs a tissue-specific epigenetic vulnerability in aggressive adrenocortical carcinomabioRxiv - Cancer Biology 2022Quote: Samples were prepared by boiling at 95°C for 6 minutes in lysis buffer supplemented with 4x Laemmli Sample Buffer (Bio-Rad), with 355 mM 2-mercaptoethanol freshly added according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... in denaturing conditions after incubation into XT-sample buffer (Bio Rad) for 5 min at 90 °C with 12% Bis-Tris Criterion XT precast gels (Biorad, USA) with 1x XT-MOPS buffer at fixed current of 25 mA for 1.5-2 h ...
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bioRxiv - Molecular Biology 2022Quote: ... 10% glycerol) at 30°C for 20 minutes in the dark and subsequently imaged using the ChemiDoc MP system (Bio-Rad). The gel was then incubated in assay buffer for an additional 10 minutes with the addition of 0.1% SDS and imaged again.
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bioRxiv - Microbiology 2023Quote: ... boiled for 5 minutes at 95°C and loaded onto Mini-Protean TGX Stain Free Gels 4-20% (Bio-Rad, P4568096). The gels were transferred onto 0.45 μm nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... and heated to 100 °C for 5 min and resolved on 4-20% (w/v) gradient SDS-PAGE gels (Bio-Rad) with Tris-glycine buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... Oocyte and embryo samples were boiled at 95 °C for 5 min and separated by SDS-PAGE using Any kD precast Polyacrylamide Gels (Bio-Rad). Blotting was performed onto a nitrocellulose membrane (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... in a 100 μL reaction for 2 hours at 37°C and purified using Micro-Bio P-30 spin columns (Bio-Rad). The radiolabeled probe was heated to 90°C for 2 minutes and resuspended in 10 mL of ULTRAhyb Oligo hybridization buffer (Thermo Fisher) ...
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Pushed to the edge: hundreds of Myosin 10s pack into filopodia and could cause traffic jams on actinbioRxiv - Cell Biology 2023Quote: ... All samples were boiled at 70°C for 10 min before loading onto a 4-20% Mini-PROTEAN TGX Stain-Free protein gel (BioRad, 4568095). The gel was run at 180 V for 45 min and imaged on a ChemiDoc ...
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bioRxiv - Microbiology 2023Quote: ... in 1X TAE buffer at 14 °C on a Bio-Rad clamped homogeneous electric field apparatus (CHEF-DR III, Bio-Rad). 3 V/cm were used with 96 h switch time of 600 s at 120° ...
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bioRxiv - Cell Biology 2023Quote: ... The lysates were centrifuged at 18,000 x g for 30 minutes at 4 °C and the supernatant was incubated with SurebeadsTM Protein A Magnetic Bead (Bio-rad) conjugated with rabbit monoclonal DYKDDDDK antibody at RT for two hours ...
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bioRxiv - Cell Biology 2023Quote: ... the lysates were mixed with an equal volume of 2X Laemmli sample buffer and incubated for 5 min at 95°C prior to separation by SDS PAGE on Criterion TGX stain-free 4-15% gradient gels (Bio-Rad). Following SDS-PAGE ...
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bioRxiv - Microbiology 2023Quote: ... 2% formaldehyde gel and separated at 125-150V at 4°C for 1-2 h and then transferred to a Zeta-Probe GT membrane (Bio-Rad) via capillary action overnight (Streit et al. ...
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bioRxiv - Cell Biology 2023Quote: ... A total of 20 μg protein was denatured at 95 °C for 5 min and loaded on a 4-20% precast polyacrylamide gel (BioRad, 4568094) for gel electrophoresis ...
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bioRxiv - Molecular Biology 2023Quote: ... About 15 μg of protein samples from lysates were boiled at 95°C for 5 min in Laemmli sample buffer (Bio-Rad) and resolved in 4-15% gradient SDS-PAGE gels (Mini-Protean TGX ...
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bioRxiv - Bioengineering 2023Quote: ... The mixture was then heated to 95 °C for 5 min and loaded into pre-cast polyacrylamide gels (Mini-PROTEAN TGX, Bio-Rad) for electrophoresis in TGS buffer (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... pre-run for 1 hour at 4 ° C and run for 1 hour at 120 volts on Mini-Protean 3 gels (Bio-Rad).
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bioRxiv - Microbiology 2022Quote: ... 20 μg of proteins of each sample or both input and eluates from the IPs were heated in Laemmli loading buffer at 95°C for 5 min and loaded on a SDS-polyacrylamide electrophoresis gel (Bio-Rad 4–20% Mini-PROTEAN® TGX Stain-Free™ Protein Gels ...
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bioRxiv - Cell Biology 2022Quote: ... The OD595 for each strain was recorded at the beginning and end of an 11-h incubation period at 30°C using an iMark Microplate Absorbance Reader (Bio-Rad). A detailed outline of the SGA procedure is included in Table S4.
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bioRxiv - Cell Biology 2023Quote: ... plates were incubated at 25 °C for 30 h before imaging on a scanner and transilluminator (Bio-Rad Gel Doc XR+).
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bioRxiv - Physiology 2023Quote: ... Samples were run 16 h at 9 °C at 4 V with a 1–10 running ratio in TBE buffer using CHEF-DRII system (Bio-Rad). After the run ...
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bioRxiv - Neuroscience 2023Quote: ... The samples were boiled for 8–10 min at 95°C and loaded onto precast 12% Bis-Tris gels (Bio-Rad) with MES SDS running buffer (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 μg protein per sample was heated to 95 °C for 5 min in sample loading buffer and separated by SDS-PAGE using the Mini-PROTEAN electrophoresis system (Bio-Rad). Proteins were transferred to a nitrocellulose membrane using the Trans-Blot Turbo transfer system (Bio-Rad) ...
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bioRxiv - Plant Biology 2023Quote: ... extracted proteins were diluted in SDS loading dye and incubated at 72°C for 10 min and were loaded to 4-20% Mini-PROTEAN TGX gels (Bio-Rad). Immunoblotting was performed with antibodies ...
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bioRxiv - Microbiology 2023Quote: ... Plates were incubated at 30°C and the growth was monitored by image scan using the device ChemiDoc MP (Bio-Rad) every 24 hours ...
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bioRxiv - Biochemistry 2023Quote: ... boiled for 5 min at 98 °C in the presence or absence of β-mercaptoethanol and loaded onto a TGX mini protean precast gel (Biorad) for 50 min at 200V.
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bioRxiv - Biophysics 2023Quote: ... This culture was used to inoculate 1 L of medium containing 25 µg/ml kanamycin in a 2 L baffled Erlenmeyer flask and grown at 37 °C in a C76 shaking water bath until the optical density at 600 nm (OD600, SmartSpec Plus spectrophotometer, Bio-Rad) reached ∼0.5 – 0.6 ...
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bioRxiv - Biochemistry 2023Quote: ... This mixture was then incubated at 4°C for 2 h with gentle agitation before reconstitution was initiated by removing detergent by incubating with Bio-beads (Bio-Rad) at 4°C overnight with constant rotation ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were then clarified through centrifugation (14′000g, 20 min, 4°C) and then quantified using Bradford Reagent (Bio-Rad Laboratories) according to manufacturer’s instructions.
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bioRxiv - Bioengineering 2023Quote: ... The samples were then boiled at 95°C for 5 minutes and 20 μL of the samples were loaded into a pre-made polyacrylamide gel (Bio-Rad) immersed in 1x Tris-Glycine-SDS Buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were boiled for 10 min at 94°C before loading on polyacrylamide gels (4-15% Bis-Tris Gel, Bio-Rad). Proteins were separated by gel electrophoreses at 80 V and transferred at 90 V for 2 h on nitrocellulose membranes (Precast Protein Gels ...