Labshake search
Citations for Bio-Rad :
751 - 800 of 5509 citations for 6 Chloro 5 6 dihydroimidazo 1 5 a pyrido 3 2 e pyrazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Physiology 2022Quote: ... diluted 1:2500 in PBS-Tween 5% milk was used to detect primary antibodies bound to the blot by BIO-RAD ChemiDoc imaging system ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cells were subsequently centrifuged at 1200 rpm for 5 minutes before their pellets being resuspended in 1 ml medium for cell counting using TC10 automated cell counter (BioRad USA) before re-plating ...
-
bioRxiv - Neuroscience 2023Quote: ... First strand cDNA samples were diluted 1:10 and 5 μL was used as template with ddPCR Supermix for Probes (No dUTP) (Bio-Rad) and TaqMan Gene Expression Assays (Life Technologies ...
-
bioRxiv - Microbiology 2024Quote: ... GFP-tagged proteins were detected using anti-GFP (Wako, mFX75, 1:5 000 dilution) antibodies combined with Goat Anti-Mouse IgG (H+L) HRP Conjugate (Bio-Rad). Images were acquired using Amersham Imager 600 (GE Healthcare ...
-
bioRxiv - Microbiology 2023Quote: ... for real-time PCR analysis of SARS-CoV-2 E genes using the iTaq Universal Probes One-Step RT-PCR Kit (Bio-Rad, USA) and the QuantStudio™ 5 Real-Time PCR System (Applied Biosystems™ ...
-
bioRxiv - Cell Biology 2019Quote: ... Proteins were resuspended in a small amount of buffer (6 M urea, 50 mM Tris–Cl pH 7.5) and quantified by the BCA quantification method (Bio-Rad, Hercules, CA). Urea concentration was reduced to 4 M and the pH was adjusted to 8.8 with 25 mM Tris–HCl ...
-
bioRxiv - Developmental Biology 2021Quote: ... Additional pre-absorption controls were performed in which the anti-IL-6 antibody was incubated overnight at 4 °C with a 10 fold molar excess of recombinant chicken IL-6 (1.67 μM; Bio-Rad Laboratories, Inc.) before immunolabeling fixed sections of chick ocular tissues ...
-
bioRxiv - Neuroscience 2019Quote: ... 5uL of the sample was loaded into 4-6% polyacrylamide gel along with Precision Plus Protein™ Dual Color Standard (#1610374S, Bio-Rad) and was run at 120 V ...
-
bioRxiv - Neuroscience 2021Quote: ... Membrane homogenates (50 μg) were separated on 6% SDS-PAGE gels and transferred onto presoaked PVDF membranes (Bio-Rad, Pleasanton, CA, USA). Blots were then blocked with 10% non-fat milk in PBST (100 nM phosphate buffer ...
-
bioRxiv - Neuroscience 2020Quote: ... Equal amounts of protein were run on 6% SDS-PAGE gels and blotted onto nitrocellulose using a semi-dry Trans-Blot (Bio-Rad, 1703940). Blots were incubated overnight at 4°C with a rabbit anti-myosin 18A polyclonal antibody that recognizes the C-terminal 18 residues of both myosin 18Aα and 18Aβ (Bruun et al. ...
-
bioRxiv - Microbiology 2020Quote: ... pH 7.4) in D2O using Micro Bio-Spin® Columns with Bio-Gel® P-6 (Bio-Rad Laboratories, Hercules, CA, USA). To avoid aggregation ...
-
bioRxiv - Bioengineering 2021Quote: ... were desalted and buffer exchanged into 10 mM ammonium acetate using a spin column (Micro Bio-Spin™ P-6 Gel Columns, Bio-Rad). Octylamine was added to aliquots of NCB solution at a concentration of 0.1% (v/v) ...
-
bioRxiv - Biochemistry 2022Quote: ... pH 7.6 with HCl) for one hour and then divided for blotting on the basis of the prestained protein ladder (Bio-Rad, 1610373). Primary antibodies (see antibody table for vendor and dilutions ...
-
bioRxiv - Molecular Biology 2022Quote: ... The bombardment was performed (a distance of 6 cm using 1350 psi rupture disks) with a PDS-1000/He Biolistic Particle Delivery system (Bio-Rad, USA). The antibiotic concentration was determined after a sensitivity test ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... was homogenized in 10 mM sodium acetate buffer (500 μL, pH 6.5) and filtered through Bio-spin 6 column (Bio-Rad, Hercules, CA) prewashed with 10 mM sodium acetate buffer (pH 6.5 ...
-
bioRxiv - Biochemistry 2020Quote: ... Purification of the desalted bacteriocin protein was performed using an Uno S-6 prepacked monolith cation exchange column (12×55 mm, Bio-Rad, USA) on a FPLC system (BioLogic DuoFlow System ...
-
bioRxiv - Biochemistry 2020Quote: ... the desalted protein fraction was purified using the Uno S6 prepacked monolith cation exchange column (12 × 53 mm, 6 mL, Bio-Rad, USA). The eluted fraction containing purified bacteriocin was concentrated to 4.6 mg/mL in an Amicon centrifugal filter concentrator with a 3 kDa cutoff membrane (Millipore ...
-
bioRxiv - Biochemistry 2022Quote: ... SDS-PAGE and size exclusion chromatography (SEC) using a Superose® 6 Increase 10/300 GL column (Cytiva) and an NGC Quest 10 Plus System (Bio-Rad) showed that both wild-type and variant proteins were pure and monodisperse (Figure 2-figure supplement 1).
-
bioRxiv - Evolutionary Biology 2023Quote: ... The qPCR was performed with a QuantStudio 6 Flex system equipped with a 384-well block and the SspAdvanced Unversal SYBR® Green Supermix (#1725272, BioRad, Switzerland) under the following conditions ...
-
bioRxiv - Plant Biology 2023Quote: ... and proteins were separated in SDS/urea–PAGE (15% polyacrylamide, 6 M urea) (Laemmli, 1970) using mini-protean apparatus (Bio-Rad USA). These separated proteins were transferred to a nitrocellulose membrane using trans-blot apparatus (Bio-Rad USA) ...
-
bioRxiv - Microbiology 2024Quote: The tRNA modified product from MenT3 in vitro reactions underwent the following steps: it was initially purified using Bio-Spin® 6 Columns (Bio-Rad), followed by incubation with a putative exoribonuclease (10 µM ...
-
bioRxiv - Genomics 2023Quote: ... The nuclei were isolated from cell homogenate by centrifugation and counted on an automated cell counter (TC20 BioRad, range 4-6 um). Transposition of 100k (weeks 1 ...
-
bioRxiv - Immunology 2022Quote: ... then blocked for 1 to 3 hr at RT with 50 μL/well of blocking buffer: 2% Blotting Grade Blocker (Bio-Rad cat. # 1706404) and 2% heat-inactivated goat serum (Gibco ...
-
bioRxiv - Bioengineering 2022Quote: ... 4 ul of diluted cDNA (1:40 dilution rate) were used for each reaction with 5 ul SsoAdvanced Universal SYBR® Green Supermix (BIO-RAD) and 1 ul of primer mix (5uM) ...
-
bioRxiv - Microbiology 2020Quote: Two hundred µL of overnight bacterial cultures were transferred 5 mL of a 1:10 dilution of TSB medium (Bio-Rad, USA) supplemented with 1 mg mL-1 L-tryptophan and incubated at 30°C overnight (Gordon & Weber 1951) ...
-
bioRxiv - Immunology 2021Quote: ... Livers were paraffin-embedded and immunohistochemistry was performed on 5-micrometer sections using F4/80 antibody clone Cl:A3-1 (BioRad Cat. No. MCA497) and the VECTASTAIN Elite ABS HRP kit (Vector Laboratories ...
-
bioRxiv - Immunology 2020Quote: ... Conjugate DNA was measured by qPCR amplification from 1 μL of lysate in a 10 μL reaction containing 5 μL of iTaq Universal SYBR Green Supermix (Bio-Rad, 1725125) and 5 pmol of each primer (Supplementary Table 1) ...
-
bioRxiv - Microbiology 2023Quote: ... Membranes were blocked in 5% BSA for 60min at RT and incubated with the primary antibody (anti-NP, Bio-Rad, 1/5000) for 45-60min shaking ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The blocking buffer was removed and 10 ml 5% BSA-PBST with 1 μl RABBIT anti-DYKDDDDK Tag antibody (Bio-Rad, # AHP1074), 1 μl anti-MBP Monoclonal Antibody (New England Biolabs ...
-
bioRxiv - Biophysics 2021Quote: ... Detergents were removed by adding 2-3 batches of Bio-beads SM2 (Bio-Rad) with constant rotation for overnight ...
-
bioRxiv - Cell Biology 2020Quote: ... 5%β-mercaptoethanol) and migrated on 4-15% Tris-glycine gradient gels (BioRad).
-
bioRxiv - Molecular Biology 2021Quote: ... and blocked in PBS/0.1%Tween20/5% Blotting-Grade Blocker (BioRad; Cat #: 1706404) for 30 minutes ...
-
bioRxiv - Immunology 2022Quote: ... which were subsequently blocked with 5% blotting grade blocker (cat# 170-6404, BioRad) powder in PBS ...
-
bioRxiv - Molecular Biology 2020Quote: ... vortexing vigorously for 5 s before pipetting in plug mould (Bio-Rad 1703713) and solidifying 15-30 min at 4°C ...
-
bioRxiv - Molecular Biology 2020Quote: ... vortexing vigorously for 5 s before pipetting in plug mould (Bio-Rad 1703713) and solidifying 15-30 min at 4°C ...
-
bioRxiv - Genomics 2020Quote: ... 5 µL of iTaqTM Universal SYBR® Green Supermix (Bio-Rad, Hercules, California) and a primer pair with a concentration of 0.22 µM each ...
-
bioRxiv - Developmental Biology 2019Quote: ... The blot was blocked with 5% Blotting Grade Non Fat Dry Milk (BioRad) and developed with 1:20,000 anti-PDI (Sigma P7496 ...
-
bioRxiv - Neuroscience 2019Quote: ... 5 μL of cDNA was mixed with SsoAdvanced SYBR Green Supermix (Bio-Rad) and indicated primers (Integrated DNA Technologies ...
-
bioRxiv - Neuroscience 2020Quote: ... Membranes were blocked for 5 minutes using undiluted EveryBlot Blocking Buffer (Bio-Rad) and then incubated with primary antibodies (specified below ...
-
bioRxiv - Physiology 2021Quote: ... then 85°C for 5 min on a C1000 thermal cycler (Bio-Rad), then stored at -20°C.
-
bioRxiv - Cell Biology 2021Quote: ... Membranes were blocked using 5% w/v non-fat dried milk (Bio-Rad) in Tris-buffered saline (TBS ...
-
bioRxiv - Microbiology 2021Quote: ... All samples were run and analyzed using the iQ™5 device (BioRad).
-
bioRxiv - Cancer Biology 2020Quote: ... for 5 min protected from light and developed on a ChemiDoc (Bio-rad). For Coomassie staining ...
-
bioRxiv - Genomics 2021Quote: ... lysates and 5 μl of Precision Plus Protein Kaleidoscope Standards (Bio-Rad, 1610375) were run on Bio-Rad 7.5% Mini-PROTEAN TGX precast gels with Tris/Glycine/SDS (Bio-Rad ...
-
bioRxiv - Biophysics 2020Quote: ... 5% glycerol (w/w) connected to an UV-Vis detector (BioRad UV 1806) and a MALS detector (Wyatt DAWN DSP ...
-
bioRxiv - Plant Biology 2021Quote: ... The membrane was blocked with 5% (w/v) Blotting-Grade Blocker (Bio-Rad) in Tris-buffered saline (TBS ...
-
bioRxiv - Neuroscience 2021Quote: ... 5-10µg protein were loaded on SDS-PAGE gels (4-15% acrylamide, Biorad). After 1h of electrophoresis at 150V ...
-
bioRxiv - Physiology 2022Quote: ... Membranes were blocked in 5% blocking-grade nonfat dry milk (Bio-Rad Laboratories) in PBS-Tween20 and incubated with primary antibodies overnight at 4°C ...
-
bioRxiv - Cancer Biology 2019Quote: ... Membranes were blocked in 5% milk in Tris-buffered saline (Bio-Rad, 1706435) with 0.1% Tween-20 (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2019Quote: ... Membranes were blocked with 5% (w/v) blotting-grade blocker (Bio-Rad Laboratories) in PBST ...