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Citations for Bio-Rad :
751 - 800 of 1370 citations for 5 NITROFURFURYL ALCOHOL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Nuclear (5 µg total protein) and cytoplasmic (10 µg total protein) fractions were boiled in Laemmli buffer (Bio-Rad), run on Criterion 4-20% Gels (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: ... 40 µL of supernatant containing 5 µg of proteins were mixed to 36 μL Laemmli Buffer 1x (Bio-Rad) and 4 µL β-mercaptoethanol BioUltra (Sigma ...
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bioRxiv - Synthetic Biology 2021Quote: ... the membrane was placed in Pierce chemiluminescence substrates for 5 minutes and imaged on film using ChemiDoc (Bio-Rad).
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed in a solution of TBS+0.01 % Tween-20 (TBS-T) and incubated in 5 % non-fat milk (Biorad) blocking solution for 2 h ...
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bioRxiv - Cell Biology 2021Quote: ... 5 to 50 µg of DNA was electroporated (375 V, 1,000 µF, 750 Ω; GenePulser Xcell; BioRad, Hercules, CA) into trophozoites ...
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bioRxiv - Cell Biology 2020Quote: ... Standard qPCR reactions with 5 ng cDNA per reaction were performed with iTaq Universal SYBR Green Supermix (Bio-Rad) in the CFX Connect Real-Time PCR Detection System ...
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bioRxiv - Cancer Biology 2022Quote: ... samples were heated for 5’ at 95°C in Lämmli buffer and protein lysates were resolved on polyacrylamide Mini-PROTEAN TGX gels (BioRad) and transferred onto nitrocellulose membrane by wet-transfer ...
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bioRxiv - Cancer Biology 2022Quote: ... Real-time PCR was performed on 5 ng/μL cDNA using iTaq Universal SYBR Green Supermix (Bio-rad, 64361172) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... Onion epidermal cells were bombarded with 5 μg of each DNA construct using a helium biolistic gun (BIO-RAD). Treated epidermal cells were kept in the dark at room temperature for 16 h before analysis by confocal microscopy (Confocal Olympus FV 1000) ...
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bioRxiv - Microbiology 2020Quote: ... purified on Ni-NTA resin (G-Biosciences, #786-940 in 5 ml Bio-Scale™ Mini Cartridges, BioRad #7324661), then dialyzed ...
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bioRxiv - Neuroscience 2020Quote: ... and blocked with 5% (w/v) non-fat milk tris-buffered saline (TBS) with 0.1% Tween-20 (Bio-Rad) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA was diluted 1/5 and analyzed by RT-qPCR using 2X iTaq Universal Sybr Green Supermix (Bio-Rad) and the oligonucleotides indicated in Table S2 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... pH 8.3) running buffer by loading 5 μg sample onto 4-20% Mini-PROTEAN® TGXTM gels (Bio-Rad) with iBrightTM Prestained Protein Ladder (#LC5615 ...
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bioRxiv - Neuroscience 2019Quote: ... Neurons were biolistically transfected after 5-6 days in vitro using a Helios Gene Gun (120 psi; Bio-Rad) with pLenti-hSyn-eNpHR3.0-EYFP (eNpHR3.0 fused to EYFP and driven by the human synapsin I promoter ...
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bioRxiv - Microbiology 2019Quote: ... the infected cell lysates were incubated for 5 min at 55°C in a S1000 thermal cycler (Bio-Rad). The total volume of each reaction was 30 μl ...
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bioRxiv - Synthetic Biology 2020Quote: ... Genomic DNA was obtained from single adults via incubation in 100µL of 5% w/v Chelex beads (BioRad Inc.) at 55°C ...
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bioRxiv - Microbiology 2020Quote: ... 20 mL blocking buffer supplemented with 5 μL of horseradish peroxidase (HRP) conjugated rabbit anti-mouse secondary antibody (BioRad) was added and incubated for 2 hours at room temperature with agitation ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and 15μg protein in 5x Laemmli buffer with 5% b-mercaptoethanol was loaded on Mini-PROTEAN TGX Precast Gels (BioRad). Gel lanes were cut into three sections for peptide extraction ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were washed 3 times with PBS and then blocked for 1 hour at room temperature with DPBS with 0.4% Triton X-100 and 5% non-fat dry milk (BioRad). Cells were stained with primary and secondary antibodies (Table S3 ...
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bioRxiv - Cell Biology 2020Quote: Immunoprecipitated proteins were resuspended in SDS-PAGE sample buffer and heated 5 minutes at 95°C before loading onto a 7.5% acrylamide mini-Protean TGX gel (BioRad). After 7 minutes of electrophoresis at 100 V the gel was stained with Imperial Protein stain (Thermo ...
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bioRxiv - Cell Biology 2019Quote: ... Protein samples were heated at 95°C for 5 minutes and 10µL were loaded on SDS-PAGE gels (4-20%Mini-PROTEAN TGX Stain-Free, BioRad). After electrophoresis ...
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bioRxiv - Systems Biology 2020Quote: ... A total of 800 µl of cell suspension mixed with 10 µg of pDM304_Red-FL2 in a 4-mm-width cuvette was subjected to electroporation (two 5 sec separated pulses with 1.0 kV and a 1.0 msec time constant) using a MicroPulser (Bio-Rad). These cells were plated on 4–6 pieces of 90-mm plastic dishes with HL5 medium and incubated at 22°C for 18 hours under the non-selective conditions and then cultured in the presence of 10 µg/ml G418 (Wako) ...
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bioRxiv - Genomics 2021Quote: ... 1 μL diluted cDNA in a total volume of 5 μL using a CFX384 Real-Time System (Bio-Rad). For each sample we conducted three technical replicates and used their mean as the data point ...
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bioRxiv - Genomics 2021Quote: ... They were then boiled for 5 minutes at 99°C after the addition of Laemmli buffer (Cat. #1410737, Biorad). Proteins were separated by SDS-PAGE and transferred to a membrane with a Trans Blot TURBO (Biorad) ...
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bioRxiv - Biochemistry 2021Quote: ... 5 μM of proteins and 3x of SYPRO™ Orange were pipetted into a 96-well PCR plate (BioRad, MicroAmp Fast 96-Well Reaction plate ...
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bioRxiv - Biochemistry 2021Quote: Nucleosome quality was analyzed by running the nucleosome on native PAGE on a 5% TBE gel in 0.5X TBE buffer (BioRad) that was run for 60 min at 150 volts ...
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bioRxiv - Biochemistry 2021Quote: ... was added to a final concentration of 1X to the quenched reaction and samples were loaded onto a 5% TBE gel and run for 60 min at 150 volts in 0.5X TBE Buffer (BioRad). Gels were stained with ethidium bromide and imaged on a BioRad ChemiDoc ...
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bioRxiv - Immunology 2022Quote: ... Internal loading control was performed by stain free visualization after 5 min activation on a ChemiDoc imaging system (BioRad). Protein transfer was done either on nitrocellulose membrane or polyvinylidene fluoride (PVDF ...
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bioRxiv - Neuroscience 2022Quote: ... 5% β-mercaptoethanol) to be run on SDS-PAGE gels using the Mini Protean system (Bio-Rad, no. 1658004). Samples were heated at 95°C for 5 minutes and loaded on 4-15% TGX gels (Bio-Rad ...
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bioRxiv - Neuroscience 2022Quote: ... for 1 minute and then imaging every 10 seconds for 5 minutes on the ChemiDoc XRS+ machine (Bio-Rad). ImageLab (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... the membrane was blocked using 5% non-fat milk in 0.1% TBS-T (1X Tris-Buffered Saline, 0.1% Tween 20, Bio-Rad) for 1 hr at RT ...
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bioRxiv - Cell Biology 2022Quote: ... and then boiled for 5 minutes before being loaded into either a precast 10% polyacrylamide gel (4561034, Bio-Rad) or manually made gel for electrophoresis with Tris-glycine-SDS running buffer (24.8 mM Tris ...
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bioRxiv - Microbiology 2022Quote: ... samples were loaded onto Mini-PROTEAN TGX Pre-cast Stain-Free gels (5-15%, BioRad Laboratories, Hercules, CA, USA) and total protein was visualised post-transfer to PVDF membranes on ChemiDoc Touch Imaging System ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 µL of the supernatant was loaded into a 4−20 % Mini-PROTEAN-TGX gel (BioRad, Hercules, CA, USA), run at 165 V for 50 minutes ...
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bioRxiv - Microbiology 2022Quote: ... Each reaction consisted of 5 μl of extracted DNA and 15 μL of iTaq™ Universal Probes Supermix (BioRad), containing 0.4 mM of each primer ...
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bioRxiv - Microbiology 2023Quote: ... both proteins were supplemented with 5 mM MgCl2 and protein concentration was determined using a colorimetric assay (Bio-Rad). Proteins were then mixed at a 2.5:1 mass ratio of KtrC to N-terminal Strep-tagged KtrA (11 mg of KtrC and 4.5 mg of N-terminal Strep-tagged KtrA in 10 mL total volume of 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2023Quote: ... pH 7.5] with 0.1% (v/v) Tween 20) containing 5% Blotting-Grade Blocker (Bio-Rad Laboratories, cat. #170-6404) for 30 mins ...
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bioRxiv - Genetics 2023Quote: ... The membrane was blocked 1 hr with 5% Blotting Grade Blocker Non-Fat Dry Milk (Bio-Rad, Cat# 1706404XTU) and incubated overnight with the primary antibodies [TTC3 Polyclonal Antibody (Abcam ...
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bioRxiv - Microbiology 2023Quote: ... the membrane was washed four times in TBS-T for 5 min each before incubation for 1 h with secondary antibody (1:2,000 dilution of BioRad affinity purified goat α-rabbit IgG conjugated to alkaline phosphatase ...
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bioRxiv - Plant Biology 2023Quote: ... Leaf lysates were cleared by centrifugation at 10,000×g for 5 min and total protein normalized via Bradford Assay (Biorad). Extracts were prepared for SDS-PAGE with a 5x loading buffer containing 10% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... To visualize the protein bands, membranes were incubated (5 min, RT) in Clarity™ western ECL substrate (Bio-Rad) and imaged using the ChemiDoc™ MP imaging system (Bio-Rad) ...
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bioRxiv - Cell Biology 2022Quote: ... The transferred membrane was briefly washed with TBST (0.1% Tween-20 in TBS) and blocked with Blocking Buffer (5% Blotting-Grade Blocker [1706404, BioRad] or 5% BSA [A9647-50G ...
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bioRxiv - Immunology 2023Quote: ... cDNA was amplified in a 10 μL reaction mixture containing 5 μL of iTaq SYBR Green Supermix (Bio-Rad) and 0.5 μM each of gene-specific forward and reverse primer ...
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bioRxiv - Molecular Biology 2023Quote: ... and 5 μg/sample was loaded onto 4-15% gradient polyacrylamide gels (Mini-PROTEAN TGX Gels, Bio-Rad, 4561083), transferred (Bio-Rad ...
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bioRxiv - Plant Biology 2023Quote: ... and 20 μl were mixed with 5 μl 5x Lämmli buffer for loading on a 10% SDS-PAGE gel (BioRad). After blotting was done on nitrocellulose using the semi-dry system (BioRad) ...
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bioRxiv - Bioengineering 2023Quote: ... cDNA was diluted 1:5 and RT-qPCR was done using SsoAdvanced™ Universal SYBR Green Supermix (Bio-Rad) with PrimePCR Assays (CCDC40 ...
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bioRxiv - Plant Biology 2023Quote: ... at 1:10,000 dilution in 5% milk PBST and ECL prime chemiluminescent reagent (Amersham: RPN2236) on a ChemiDoc gel imager (Biorad).
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bioRxiv - Physiology 2023Quote: ... Total protein (5∼15 µg) was loaded and separated by 4–20% pre-made SDS-PAGE gels (#4568095, BioRad, Mini-PROTEAN TGX Stain-Free Precast Gels ...
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bioRxiv - Cell Biology 2023Quote: ... Equal amounts of protein samples (5-20µg) were diluted in RIPA buffer and βME-Laemmli buffer (Bio-Rad Laboratories) to equal volumes and boiled for 7 minutes at 95°C ...
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bioRxiv - Biochemistry 2023Quote: ... The soluble fraction was loaded onto a 5 mL Bio-Scale Mini Nuvia IMAC Ni-charged Cartridges (Bio-Rad) which was pre-equilibrated with 5 column volumes (CV ...